神经生长因子基因转染联合强化铁营养防治豚鼠爆震性聋的实验研究

Objective To study the protective effects of adenovirus-mediated human beta-nerve growth factor gene (hNGFβ) transfer combined with iron fortified nutrition on blast hearing damage in guinea pigs. Methods Deafness was induced by blast (172dB SPL) in 35 healthy guinea pigs. Seven days after noise exposure, 10 guinea pigs were inoculated with adenovirus-mediated hNGFβ (Ad-hNGFβ) into the perilymphatic space (the gene group), another 10 guinea pigs were given hNGFβ combined with iron fortified nutritional diet (the combination group), still another 10 guinea pigs were inoculated with artificial perilymphatic fluid (APF), and 5 guinea pigs served as control, given neither medicine nor noise exposure. Auditory brainstem response (ABR) threshold shifts were monitored in the guinea pigs before blast exposure and after gene transfer. Changes in cochlear morphology were examined with immunohistochemical and HE staining. Results One week after successful inoculation of hNGFβ, it was noted that Ad-hNGFβ protein was expressed in each turn of the cochlea, and its intensity was almost equal. After gene transfer in the combination group and the gene group, ABR threshold shifts at week 1 and 4 were significantly smaller than those of the APF group. HE staining showed that the number of the spiral ganglion cells in the combination group and the Ad-hNGFβ group was significantly higher than that of the control group after 4 weeks (P<0.01). Conclusions Ad-hNGFβ combined with iron fortified nutritional diet was effective in the prevention and treatment of blast hearing damage of cochlear hair cells.     

大鼠听性脑干反应和耳蜗电图记录法

我科对大鼠听性脑干反应(ABR)和耳蜗电图(ECoG)记录进行了长期探索,成功地描记了大鼠ECoG和ABR曲线。经过6年多实际应用,证明此记录法简便、稳定可靠。现报告如下:     

人血浆高敏感性C反应蛋白与代谢综合征相关性分析

目的:了解我国人群血浆高敏感性C反应蛋白(hs-CRP)水平与代谢综合征(MS)关系及其它影响hs-CRP水平的相关因素。方法:1198例血浆标本来自2006年3月~2006年11月在浙江省桐乡市第三人民医院体检人群。采用Beckm an LX20血生化全自动生化仪及其配套试剂,测定上述空腹血浆标本中血糖(FPG)、甘油三脂(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-c)、低密度脂蛋白胆固醇(LDL-c)和hs-CRP水平。MS诊断标准参照中华医学会推荐标准。采用SPSS13.0统计学软件,分析hs-CRP水平与MS关系及其它影响hs-CRP水平的相关因素。结果:男性hs-CRP水平(x±s=1.55±0.53)高于女性(x±s=1.32±0.56)(P<0.001)。15.3%被检者(183/1198)患有MS,其hs-CRP水平明显高于非MS者(P<0.001)。各MS判断指标中,分别有肥胖、高血压病、糖尿病、高TG、低HDL-c时,其hs-CRP水平也明显升高(P<0.001)。排除年龄、性别影响后,hs-CRP水平与MS显著相关(OR值2.18,P<0.001)。结论:hs-CRP水平与MS密切相关,hs-CRP水平增高可作为独立反映MS发生的标志性检测指标。     

循环障碍对耳蜗的影响

为阐明基底动脉系统循环障碍对耳蜗功能的影响,本研究对阻断动脉时的耳蜗动作电位(AP)和耳蜗血流变化进行了实验观察。用猫33只,戊巴比妥钠腹膜内注射麻醉、切除喉、下咽、长肌和枕骨的一部分,暴露脑干腹侧,阻断基底动脉、前小脑动脉和迷路动脉,激光多普勒血流计测定阻断动脉同侧的耳蜗血流,耳蜗电图记录对侧AP变化作为对照。结果发现,阻断基底动脉、前小脑动脉和迷路动脉时     

人巨细胞病毒感染与上颌窦鳞癌

人类巨细胞病毒 (Ｈｕｍａｎｃｙｔｏｍｅｇａｌｏｖｉｒｕｓ ,ＨＣＭＶ )与人类肿瘤的关系已成为肿瘤病毒学中一个重要的研究领域。大量的分子生物学和流行病学研究结果表明 ,ＨＣＭＶ与生殖道肿瘤及口腔肿瘤关系密切[1,2 ] 。有关人乳头状瘤病毒 (Ｈｕｍａｎｐａｐｉｌｌｏｍ     

法莫替丁雷尼替丁治疗56例十二指肠溃疡近期疗效对照观察

法莫替丁、雷尼替丁是H_2 受体阻滞剂,对胃酸分泌具有明显的抑制作用.近年来广泛应用于消化性溃疡的治疗.笔者从1991年以来应用法莫替丁、雷尼备丁治疗十二指肠球部溃疡100多例,将其中资料完整的56例分为两组,进行对照观察,现将近期疗效结果报告如下:     

Adenovirus-mediated human β-nerve growth factor gene transfer has a protective effect on cochlear spiral ganglion after blast exposure

Objective：To study whether adenovirus-mediated human β-nerve growth factor （Ad-hNGFβ） gene has any protective effect on blast hearing impairment. Methods：Deafness was induced by blast exposure （172.0 dB） in 30 healthy guinea pigs. On day 7 of blast exposure, Ad-hNGFβ was infused into the perilymphatic space of 20 animals as the study group （hNGFβ group）, and artificial perilymph fluid （APF） was infused into the perilymphatic space of the other 10 animals as the control group. At weeks 1, 4 and 8 after blast exposure, the animals were sacrificed and the cochleae were removed for immunohistochemical and HE stainings. Results： Expression of Ad-hNGFβ protein was detected in each turn of the cochlea at the 1st week, with almost equal intensity in all turns. At the 4th week, the reactive intensity of the expression of Ad-hNGFβ protein decreased. At the 8th week, no expression was detectable. The results of HE staining showed that the amount of spiral ganglions in hNGFβ group was significantly greater than that of the control group at week 4 （P〈0.01）. Conclusion： Ad-hNGFβ can be expressed at a high level and for a relatively long period in the blast impaired cochlea, suggesting that Ad-hNGFβ has a protective effect on cochlear spiral ganglion cells after blast exposure and the efficient gene transfer into cochlea had been achieved without toxicity.     

鼻腔巨大息肉致鼻中隔大穿孔并促发高血压1例

患者女 ,73岁。于 10年前感冒后出现鼻塞 ,通气不畅 ,未经药物治疗。此后症状逐渐加重伴嗅觉减退。近 1年来出现头痛、头晕 ,无脓涕 ,有少量黏液性分泌物 ,偶有少许血丝。自服消炎药物及用滴鼻净点鼻无效来诊 ,诊为鼻息肉 (双 )收入院。体检 :Ｔ36 6℃ ,Ｐ80次 /ｍｉｎ ,ＢＰ32 / 13ｋＰａ。全身检查未见异常。外鼻畸形呈蛙鼻 ,双侧鼻腔可见灰白色新生物 ,不易出血。右鼻可见黏液性分泌物 ,无脓液 ,无臭味 ,各鼻窦区无压痛。鼻窦ＣＴ示右侧鼻腔外侧至左侧鼻腔充满软组织密度影 ,鼻窦未见异常。ＣＴ诊断为鼻腔占位病变 ,临床诊断为鼻息肉。…     

铁缺乏对大鼠畸变产物耳声发射的影响

目的 观察铁缺乏大鼠畸变产物耳声发射（DPOAE）的一般特征及幅值变化,探讨铁缺乏对DPOAE的影响机制。方法 18只大鼠随机分为正常对照及实验组,缺铁饮食饲养法制备动物模型,实验及动物处死前检测DPOAE,并进行耳蜗肌动蛋白免疫组化检测,分析DPOAE变化及可能机制。结果 铁缺乏组大鼠20个测试耳中,12耳2．0kHz以上频率点的DPOAE幅值略有降低,耳蜗免疫组化检测显示其外毛细胞的肌动蛋白免疫组化无明显变化;另8耳2．0kHz以上频率点的幅值下降明显,尤以2．0kHz处为,相应的耳蜗免疫组化染色显示外毛细胞肌动蛋白免疫反应活性明显降低。结论 铁缺乏可影响DPOAE幅值,其机制可能与耳蜗听毛细胞肌动蛋白免疫反应活性降低及组织缺氧有关。