Phenotypes and spontaneous cell cytotoxicity of mononuclear cells from patients with seronegative spondyloarthropathies: Ankylosing spondylitis,psoriatic arthropathy and pauciarticular juvenile chronic arthritis — Analysis of mononuclear cells from peripheral blood,synovial fluid and synovial membranes

Summary T-cell subpopulations and natural killer (NK) cells from peripheral blood, synovial fluid and synovial membranes from patients with seronegative spondyloarthropathies were investigated. Thirty-four patients with ankylosing spondylitis, sixteen patients with psoriatic arthropathy and six patients with pauciarticular juvenile chronic arthritis were studied. All the patient groups had normal proportions of T4⁺ and T8⁺ cells as well as normal T4/T8 ratios in peripheral blood. In the synovial fluids the T4/T8 ratios were reduced in ankylosing spondylitis and psoriatic arthropathy (p<0.05). Although both the T4 and T8 subpopulations were reduced, the T4/T8 ratios in the synovial membranes of patients with these two disorders tended to be within the normal range of that of peripheral blood. Increased numbers of T-cells in the synovial fluid from patients with ankylosing spondylitis expressed class II MHC antigens. The natural killer cell activity was normal in peripheral blood and synovial fluids of patients with ankylosing spondylitis and psoriatic arthropathy while it tended to be reduced, although not significantly, in pauciarticular juvenile chronic arthritis. Synovial membranes were almost devoid of NK cell activity. The number of Leu 7⁺ cells were reduced in synovial fluid of patients with psoriatic arthropathy (p<0.04), but not as significantly as in the two other patient groups.     

维生素 D_3对大鼠的免疫调节作用研究

观察多次注射维生素 D_3对大鼠的免疫调节作用,结果证明维生素 D_3对抗体产生有双相调节作用,并可增强单核巨噬细胞的分泌功能。连续使用大剂量维生素 D_3可使 T 细胞数量减少。     

乙型肝炎患者可溶性白细胞介素2受体水平变化

本研究应用酶联免疫吸附试验(ELISA)检测了103例乙型肝炎各型患者及26倒乙肝病毒(HBV)携带者血清中可溶性白细胞介素2受体(sIL-2R)水平,并结合临床资料进行分析。结果发现:各型患者及HBV 携带者sIL-2R 水平均明显高于正常对照(P<0.01～0.001),其升高程度与病情轻重呈正比,在去除危重患者酶—胆分离时谷丙转氨酶(ALT)过低的影响后,sIL-2R 水平与ALT 有良好的正相关关系(r=0.38.p<0.05);作者认为sIL-2R 能够较好地反映疾病的活动状况,动态观察血清sIL-2R 水平的变化,对判断和评价HBV 感染后的病情演变及免疫失衡状态具有重要意义。     

GITR及其在免疫调节中的作用

糖皮质激素诱导的TNFR家族相关受体（GITR），也被称为TNFRSF18、AITR（人类）。静止T细胞低水平表达GITR，而CD4^＋CD25^＋调节性T细胞则呈高水平表达。GITR与其配体（GITRL）结合后会增强T细胞激活、增殖、分泌细胞因子、MAPKs和NF-κB激活效应、抑制CD4^＋CD25^＋Treg细胞的功能，从而加强效应性T细胞的活性，有利于增强抗肿瘤免疫和抗病毒免疫。随着生物学环境的变化，GITR激活Siva或者TRAF，起着促进或诱导凋亡的作用。     

转基因小鼠高表达的FasL对Sertoli细胞免疫调节功能的影响

目的 :研究转基因小鼠高表达的FasL对Sertoli细胞在睾丸局部感染时的免疫调节作用的影响。方法 :将溶脲脲原体 (UU)分别注入FasL转基因及野生型小鼠膀胱 ,模拟上行性感染的途径。分别在感染后 1、2和 3wk处死小鼠 ,分离睾丸组织观察其病理变化 ,并用免疫组化染色法比较UU感染前后 ,Ser toli细胞上FasL和TGF β、IL 1α、IL 6的表达及分泌格局的差异。从野生型小鼠睾丸组织中分离高纯度的Sertoli细胞 ,与未感染UU的对照组相比 ,观察UU感染后FasL Sertoli细胞介导Fas Jurkat细胞的凋亡能力的变化。结果 :UU感染组的转基因小鼠 ,睾丸组织发生的病理改变比野生型更为明显 ;两种小鼠感染后Sertoli细胞分泌的调节因子变化格局不同 ;感染后Sertoli细胞对Jurkat细胞的杀伤能力增强。结论 :在抗感染免疫中 ,转基因表达的FasL可影响Sertoli细胞分泌细胞因子的格局 ,进而影响睾丸局部的免疫平衡。过高表达的FasL对机体的抗感染应答并非一定有利。     

JM细胞培养上清对人B淋巴细胞和小鼠脾细胞增殖的免疫调节作用

JM 是来自人胸腺不成熟 T 细胞的急性淋巴细胞白血病细胞系.本文以 SAC 刺激的人外周血纯化 B 淋巴细胞和小鼠脾细胞增殖为模型,观察了 JM 细胞培养上清(SPN_(JM))对人 B 淋巴细胞和小鼠脾细胞增殖的免疫调节作用.发现具有 T 细胞抑制活性的 SPN_(JM)对人和小鼠 B 淋巴细胞的增殖具有促进作用。在无 SAC 诱导时,SPN_(JM)与 HrIL—2协同对 B 细胞仍有促增殖作用.本实验还发现,高浓度时对 T 细胞具有抑制作用的 SPN_(JM),在低浓度时(1:640)对 T 细胞增殖亦具有促进作用.     

Peptides derived from IgE heavy chain constant region induce profound IgE isotype-specific tolerance

(BALB/c × SJL)F1 mice, perinatally injected with peptide-N-glyconase F-treated, deglycosylated IgE heavy chain or recombinant IgE heavy chain (CH?2-CH?4), were profoundly inhibited in antigen-specific IgE production. There exist minimally two tolerogenic IgE peptides, residing in the CH?2 and CH?4 domains. Peptide I, generated by V8 protease, comprises 39 amino acids within CH?2, beginning at amino acid 103. Peptide E begins at amino acid 312 of the CH?4 domain and extends through the CH?4 domain. The total lack of antigen-specific IgE responses in IgE peptide-treated mice was not due to overproduction of interferon-γ, nor lack of interleukin (IL)-4, as predicted by the Th2/IL-4 paradigm for IgE production. IgE-tolerant mice exhibited comparable levels of circulating anti-IgE antibodies to those of PBS-treated control mice. IgG obtained from sera of both sources failed to inhibit IgE responses in vitro. Moreover, IgE responses of spleen cells from IgE peptides-treated mice were restored by CD4⁺ T cells from PBS-treated control mice. We hypothesize that regulation of antigen-specific IgE responses is mediated by CD4⁺ T cells which normally recognize IgE peptides on IgE precursor B cells, and can be rendered tolerant by perinatal IgE peptide treatment.     

卡介菌多糖核酸对T细胞集落形成及IL－2受体表达的调节作用

本文用体外实验方法，研究ＢＣＧ－ＰＳＮ对ＴＬ－ＣＦｕ形成及ＩＬ－２Ｒ表达的调节作用。结果表明，当ＢＣＧ－ＰＳＮ浓度为８０μｇ／ｍｌ时，ＴＬ－ＣＦＵ形成及ＩＬ－２Ｒ表达均达到峰值，对ＩＬ－２Ｒ表达的调节于７２ｈ达最高水平，并且．ＴＬ－ＣＦＵ形成和ＩＬ－２Ｒ表达均显著高于对照组。提示ＢＣＧ－ＰＳＮ能增进Ｔ淋巴细胞功能。     

C3d-P28增强乙型肝炎病毒特异性基因免疫效果的研究

目的　观察补体C3d P2 8对基因免疫的调节作用及其不同拷贝数对调节作用的影响 ,为增强基因免疫效果寻求新方法。方法　PCR法获得补体C3d P2 8编码基因并以头尾串连方式将1～ 4拷贝C3d P2 8编码基因克隆至pVAON33,构建pVAON33 P2 8.[1～ 4 ]重组质粒 ,然后将HBV preS2 S编码基因分别插入pVAON33和pVAON33 P2 8.[1～ 4 ]质粒获得pVAON33 S2 S和pVAON33 S2 S P2 8.[1～ 4 ]重组质粒。肌肉注射各重组质粒DNA(每只 10 0 μg 10 0 μl)初次免疫小鼠 ,并以pVAON33为对照 ;12周后皮下注射HBsAg蛋白加强免疫各组小鼠 ,ELISA法检测免疫小鼠血清特异性抗 HBs IgG。结果　pVAON33 S2 S重组质粒免疫小鼠可诱导产生特异性抗 HBs IgG ,含不同拷贝C3d P2 8编码基因的重组质粒可诱导更高的特异性抗体 ,其中pVAON33 S2 S P2 8.4重组质粒诱导的抗体水平最高 (P <0 .0 1)。蛋白加强免疫后 ,含C3d P2 8编码基因重组质粒免疫组抗 HBs IgG迅速上升 ,并明显高于pVAON33 S2 S重组质粒免疫组 (P <0 .0 5 ) ,pVAON33 S2 S P2 8.4重组质粒诱导的抗体仍维持最高水平。结论　不同拷贝的C3d P2 8能不同程度地增强HBV preS2 S基因免疫诱导的特异性体液免疫及其蛋白加强后的回忆反应 ,其中 4拷贝C3d P2 8的增强作用较为显著。     

Correlation of interleukin-2 receptor and neopterin secretion in rheumatoid arthritis

Summary In rheumatoid arthritis (RA) an immuno dysregulation alters the release of neopterin from human monocytes/macrophages, probably by activation of a feed-back regulation mechanism in pterin metabolism. With the overactivation of T-lymphocytes, the secretion of interleukin-2 and its receptor (IL-2R) is influenced. Investigation on 84 RA patients has shown that this immunoactivation is accompanied by an increased secretion of neopterin and soluble IL-2R in the serum, and that both parameters are very suitable for detection of inflammatory disease activity. The significant correlation between neopterin and IL-2R (r=0.65; p0.001) in RA points out a possible connection in pathomechanisms of the immune system.