携RHD-CE(2-9)-D基因并产生抗-D、抗-E抗体1例

RHD-CE(2-9)-D属融合基因[1,2],为RHD基因的一种弱表现形式,常规血清学试验常将其鉴定为RhD–。据文献报道,Rh血型系统D抗原抗原性强于E抗原[3-6],理论上机体接受D抗原和E抗原免疫性刺激后产生的抗体常规应该是抗-D强于抗-E。然而,我们临床工作中遇到了抗-E(1∶512)强于抗-D(1∶64),与常规不符,探究详情报道如下。     

女性生殖细胞发育过程的分子遗传学研究进展

女性生殖细胞的发育主要经历胚胎发育期和青春期后的卵泡发育期,其中有丝分裂-减数分裂转换、减数分裂阻滞和再激活是发育过程中的关键阶段。各发育阶段独具特征性分子事件,其顺序发生亦需严密的基因调控及与性腺体细胞的交互作用。近年来,单细胞转录组学研究揭示了生殖细胞及性腺体细胞的阶段特异性表达基因、信号传导通路及表观遗传学变化,为深入理解生殖细胞发育过程、阐明相关疾病的发病机制以及促进生殖遗传研究成果的临床转化提供了科学依据和理论基础。     

一例额缝早闭的产前诊断及遗传学分析

目的探讨产前超声结合染色体核型分析以及单核苷酸多态性微阵列(single nucleotide polymorphism array,SNP-array)技术对l例疑似额缝早闭胎儿的确诊过程,实现产前诊断技术的有效联合应用。方法应用影像学检查、羊水染色体核型分析和SNP-array技术以及尸体解剖对胎儿进行检测及验证。结果胎儿羊水染色体核型分析未发现异常,SNP-array分析结果显示9p23p22.2区段存在长度7.8 Mb的缺失;父母双方核型分析以及SNP-array分析均未发现异常,胎儿为新发突变。并通过尸体解剖证实额缝早闭的诊断。结论结合临床超声和SNP-array分析确诊胎儿患有额缝早闭,尸体解剖明确诊断,为患者提供了准确的产前诊断和遗传咨询。     

乳腺癌保乳手术与重建策略

随着乳腺外科的发展，乳腺癌手术治疗术式有了更多选择。对于早期乳腺癌病人，保乳手术仍应是首选手术方式之一，肿瘤整形保乳可以扩大该手术的适应证。对于缺乏保乳指征的病人，应合理选择乳房重建手术的方法和时机。乳房重建手术可分为即刻重建、延期重建和即刻-延期重建3种术式。具体方法包括植入物重建、自体皮瓣重建和脂肪移植等。对称性手术也应纳入乳房重建策略制定之中。     

青少年女性生殖器发育异常致急腹症的围术期护理

总结66例青少年女性生殖器发育异常致急腹症患者的围术期护理体会。护理重点:给予针对性心理护理及隐私保护,以提高患者治疗信心;做好围术期疼痛管理;指导和教育患者正确使用模具,以预防术后阴道粘连和挛缩;术后密切观察及时发现阴道出血和感染等并发症。1例阴道闭锁患者因合并子宫畸形行子宫切除术;65例患者术后月经均正常来潮、经期无明显腹痛。     

四月福建党史上的今天

1966年4月4日至11日中共福建省委“小三线”建设领导小组在福州召开“小三线”建设工作会议。2015年4月8日国务院印发通知,批准《中国(福建)自由贸易试验区总体方案》。1932年4月11日至12日毛泽东在龙岩主持会议,部署攻打漳州计划。4月20日,毛泽东率领工军东路军攻克漳州,巩固发展了闽西苏区,为第四次反“围剿”斗争做了物资准备。     

Shenling Baizhu Powder (参苓白术散) Ameliorates Pi (Spleen)-Deficiency-Induced Functional Diarrhea in Rats

Objective To explore the mechanism of Pi(Spleen)-deficiency-induced functional diarrhea(FD)model rats treated by Shenling Baizhu Powder(参苓白术散,SBP).Methods Thirty male Sprague-Dawley rats were randomly divided into 5 groups including control,model,low-,medium-,and high-dose SBP groups(SBPLDG,SBPMDG,SBPHDG),6 rats in each group,respectively.Pi-deficiency-induced FD rats model was developed through Radix et Rhizoma Rhei gavage for 7 days.After modeling,the rats were treated with 3 doses of SBP[0.93,1.86,and 3.72 g/(kg·d)],and the rats in the control and model groups were given pure water for 7 days.The diarrhea index was calculated.On the 7th and 14th days,the traveled distance of rat was measured by the open field test.Serum D-xylose content was determined by the phloroglucinol method and interleukin(IL)-10 and IL-17 levels were measured using an enzyme-linked immunosorbent assay kit.The content of Treg cells was determined by flow cytometry.Results Compared with the control group,the diarrhea index and IL-17 level in the model group were significantly higher and the total exercise distance and D-xylose content significantly decreased(P>0.05).The expression of IL-10 in the SBPHDG group was significantly up-regulated,and serum D-xylose level and Treg cells increased significantly compared with the model group(P>0.05).Conclusion High-dose SBP exhibited ameliorating effects against Pi-deficiency induced FD,which might be attributed to its modulations on intestinal absorption function as well as adaptive immunity in mesenteric lymph nodes of rat.     

Patient-derived non-small cell lung cancer xenograft mirrors complex tumor heterogeneity

Objective:Patient-derived xenograft(PDX)models have shown great promise in preclinical and translational applications,but their consistency with primary tumors in phenotypic,genetic,and pharmacodynamic heterogeneity has not been well-studied.This study aimed to establish a PDX repository for non-small cell lung cancer(NSCLC)and to further elucidate whether it could preserve the heterogeneity within and between tumors in patients.Methods:A total of 75 surgically resected NSCLC specimens were implanted into immunodeficient NOD/SCID mice.Based on the successful establishment of the NSCLC PDX model,we compared the expressions of vimentin,Ki67,EGFR,and PD-L1 proteins between cancer tissues and PDX models using hematoxylin and eosin staining and immunohistochemical staining.In addition,we detected whole gene expression profiling between primary tumors and PDX generations.We also performed whole exome sequencing(WES)analysis in 17 first generation xenografts to further assess whether PDXs retained the patient heterogeneities.Finally,paclitaxel,cisplatin,doxorubicin,atezolizumab,afatininb,and AZD4547 were used to evaluate the responses of PDX models to the standard-of-care agents.Results:A large collection of serially transplantable PDX models for NSCLC were successfully developed.The histology and pathological immunohistochemistry of PDX xenografts were consistent with the patients’tumor samples.WES and RNA-seq further confirmed that PDX accurately replicated the molecular heterogeneities of primary tumors.Similar to clinical patients,PDX models responded differentially to the standard-of-care treatment,including chemo-,targeted-and immuno-therapeutics.Conclusions:Our established PDX models of NSCLC faithfully reproduced the molecular,histopathological,and therapeutic characteristics,as well as the corresponding tumor heterogeneities,which provides a clinically relevant platform for drug screening,biomarker discovery,and translational research.     

~(60)Co-γ射线辐照对黄精中微生物及多糖的影响

目的研究~(60)Co-γ射线辐照对黄精中微生物数量及多糖含有量的影响。方法参照2015年版《中国药典》方法,检测需氧菌总数(TAMC)、霉菌和酵母菌总数(TYMC)、耐热菌数、大肠埃希菌;采用硫酸蒽酮比色法,检测波长582 nm,于辐照后0、1、3、6月进行相应检测。结果黄精经0.5～6.0 kGy ~(60)Co-γ射线辐照后,微生物数量显著减少,并随着辐照剂量增加而更明显;多糖含有量显著降低,也随着辐照剂量增加而更明显。结论 3.0～6.0 kGy ~(60)Co-γ射线辐照能有效杀灭黄精中微生物,但也可能使多糖含有量降低。