Parkinson''''s Disease and Smoking: An Integral Part of PD''''s Etiological Study

INTRODUCTION Parkinson抯 disease (PD), a debilitating neurodegenerative disorder, is featured with bradykinesia, resting, muscular rigidity, gait disturbances, and postural reflex impairment[1]. PD is rare before age of 50 years, but it increases dramatically with older ages, with peak onset occurring during the age of 70-85 years. In the United States, prevalence of PD in all age groups is approximately 150 per 100 000 and is roughly 30 per 100 000 at age of less than 50 years and 800 …     

硅胶薄膜囊预防椎体后凸成形术中骨水泥渗漏的实验研究

目的探讨硅胶薄膜囊预防椎体后凸成形术中骨水泥渗漏的可能性和有效性。方法取6具甲醛固定的老年女性脊柱标本(T12～L5)制成36个单椎体,刮匙在椎体前3/4制成体积约为6ml单侧或双侧空腔,分别直接注入骨水泥和先置入壁厚100μm、200μm的硅胶薄膜囊后再注入骨水泥。结果壁厚100μm的硅胶薄膜囊包裹骨水泥可控制骨水泥在椎体内的分布,和直接注入骨水泥一样能较好地嵌入到周围骨小梁内,不形成界面。而壁厚200μm的硅胶囊虽能控制骨水泥在椎体内分布,但会在骨小梁间形成界面。结论球囊扩张椎体后凸成形术中置入壁厚100μm的硅胶薄膜囊包裹骨水泥可控制椎体内骨水泥分布,并能嵌入到骨小梁间隙,不形成界面,有效预防球囊扩张椎体后凸成形术并发症的出现。     

瘦素对骨代谢影响研究进展

瘦素及其受体在中枢和外周多个部位均有表达,具有多种生物学功能,对于骨代谢有着重要的影响作用.近来研究发现瘦素可促进人骨髓间质细胞向成骨细胞分化,抑制其向脂肪细胞分化;血清瘦素水平还与成骨细胞活性及骨骼发育相关.瘦素在外周作用于多种骨细胞促进成骨,在中枢系统则抑制骨形成.瘦素在妇女绝经后骨质疏松的发生中发挥一定作用.该文主要归纳介绍瘦素调节骨代谢基本作用及调控骨骼生长作用的一些较重要的研究发现,并对其机制作初步探讨.     

CLINICAL EFFECTS ON 149 CASES OF INFANTILE DIARRHEA TREATED WITH RAPID-STICKING MOXATHERAPY Attaching 35 contrasted cases with western medicine

Of 149 cases.infantile diarrhea were treated with Zhou's multiapplied rapid-sticking herbomoxa-pen.The main acupoints selected were Auriculoapex,Tianshu(ST 25),Zusanli(ST 36),Shenshu(BL 23),etc.TWO groups were observed,32 cases from the 149 cases as therapid-sticking moxatherapy(RSMT)group who suffered from the acute diarrhea without any thera-peutics before,and the other 35 cases with western medicine as the control group.Results showedthat the recovering rate and the average therapeutic course of healing in RSMT group were higher andshorter than that in control one(P<0.05;P<0.01),even without marked difference of the total ef-fective rates between the two groups(P>0.05).Of 149 cases with RSMT,the therapeutic effect onacute ones was better than those on delayed and chronic ones(P<0.05),yet the difference was notmarked in therapeutic effects between the etiological causes of infections and non-infections(P> 0.0.5).It is therefore worthy of popularizing RSMT due to its remarked curative results without anyside-effect,simply-manipulated and well-accepted features.     

Remissions in maternal depression and child psychopathology: a STAR*D-child report

Context  Children of depressed parents have high rates of anxiety, disruptive, and depressive disorders that begin early, often continue into adulthood, and are impairing. Objective  To determine whether effective treatment with medication of women with major depression is associated with reduction of symptoms and diagnoses in their children. Design  Assessments of children whose depressed mothers were being treated with medication as part of the multicenter Sequenced Treatment Alternatives to Relieve Depression (STAR*D) trial conducted (between December 16, 2001 and April 24, 2004) in broadly representative primary and psychiatric outpatient practices. Children were assessed by a team of evaluators not involved in maternal treatment and unaware of maternal outcomes. Study is ongoing with cases followed at 3-month intervals. Setting and Patients  One hundred fifty-one mother-child pairs in 8 primary care and 11 psychiatric outpatient clinics across 7 regional centers in the United States. Children were aged 7 to 17 years. Main Outcome Measures  Child diagnoses based on the Kiddie Schedule for Affective Disorders and Schizophrenia; child symptoms based on the Child Behavior Checklist; child functioning based on the Child Global Assessment Scale in mothers whose depression with treatment remitted with a score of 7 or lower or whose depression did not remit with a score higher than 7 on the Hamilton Rating Scale for Depression. Results  Remission of maternal depression after 3 months of medication treatment was significantly associated with reductions in the children's diagnoses and symptoms. There was an overall 11% decrease in rates of diagnoses in children of mothers whose depression remitted compared with an approximate 8% increase in rates of diagnoses in children of mothers whose depression did not. This rate difference remained statistically significant after controlling for the child's age and sex, and possible confounding factors (P = .01). Of the children with a diagnosis at baseline, remission was reported in 33% of those whose mothers' depression remitted compared with only a 12% remission rate among children of mothers whose depression did not remit. All children of mothers whose depression remitted after treatment and who themselves had no baseline diagnosis for depression remained free of psychiatric diagnoses at 3 months, whereas 17% of the children whose mothers remained depressed acquired a diagnosis. Findings were similar using child symptoms as an outcome. Greater level of maternal response was associated with fewer current diagnoses and symptoms in the children, and a maternal response of at least 50% was required to detect an improvement in the child. Conclusions  Remission of maternal depression has a positive effect on both mothers and their children, whereas mothers who remain depressed may increase the rates of their children's disorders. These findings support the importance of vigorous treatment for depressed mothers in primary care or psychiatric clinics and suggest the utility of evaluating the children, especially children whose mothers continue to be depressed.      

脊髓缺血再灌注中ERK1／2作用的初步研究

目的:研究细胞外信号调节激酶1/2(extracellular signal-regulated kinases1/2,ERK1/2)在脊髓缺血再灌注中的作用及机制.方法:观察不同时间段脊髓缺血再灌注标本的形态学改变,Westem blot检测脊髓标本中ERK1/2的磷酸化,免疫组织化学染色分析磷酸化ERK1/2(pERK1/2)在神经细胞内的定位表达.结果:脊髓缺血30 min/再灌注2 h ERK1/2发生磷酸化,并持续至再灌注24 h;对照组、脊髓缺血30 min/再灌注5 min至再灌注1 h,免疫组织化学染色未检测到pERK1/2在运动神经细胞内表达,脊髓缺血30 min/再灌注2 h至再灌注24 h,pERK1/2在运动神经细胞胞浆及胞核内均表达,且胞浆内表达异常显著.结论:脊髓缺血再灌注时ERK1/2发生磷酸化,pERK1/2过度滞留于细胞浆可能与脊髓缺血再灌注损伤引发的神经细胞凋亡有关.     

Purification and Immunity Analysis of Recombinant 6His-HPT Protein Expressed in E.coli

INTRODUCTION Selective marker genes are important for the production of genetically modified organisms (GMOs), and they are essential for the selection of modified cells at relatively low frequencies[1]. Marker genes are used to 搕ag?genes of interest and comprise several classes. Antibiotic resistance gene is one of the most commonly used markers in the modification process. The enzyme Hygromycin B Phosphotransferas (HPT) is a widely used selective marker in various animal a…