Protective effect of icaritin on focal cerebral ischemic–reperfusion mice

Objective: To investigate the protective effects of icaritin(ICT),one of the active ingredients in Epimedii Folium,on mouse model of cerebral ischemia-reperfusion(I/R)in vivo.Methods: ICR mice were subjected to an 1 h transient middle cerebral artery occlusion(MCAO)and followed by 24 h of reperfusion.Neurological deficits,infarct volume,brain edema and survive rate were measured,respectively.The levels of brain IL-1β,TNF-α,ROS and DNA-binding activity of NF-κB p65 were measured by ELISA kits.The levels of malondialdehyde(MDA)and activities of superoxide dismutase(SOD)were detected by spectrophotometry,and the release of nitric oxide(NO)were detected by Griess kit.Results: ICT markedly reduced the neurological deficit scores,brain edema,infarct volume and increased the survival rate of the cerebral I/R mice.The expression of IL-1 β,TNF-α,NO,MDA and DNA-binding activity of NF-κB p65 were significantly inhibited by ICT,while the activity of SOD were up-regulated at the same time.Conclusion: ICT possessed significant neuroprotective effects in cerebral I/R mice,which might be related to prevent neuroinflammatory and oxidative damage.     

New coumarin glucopyranosides from roots of Angelica dahurica

Objective: To investigate the chemical constituents from the roots of Angelica dahurica.Methods: The chemical constituents were isolated and purified by AB-8 macroporous adsorption resin and Sephadex LH-20 column as well as semi-preparative reversed phase HPLC.The chemical structures were identified by spectral data.Results: Ten compounds were isolated and identified as xanthoarnol-3-O-β-d-glucopyranoside(1),angedahuricoside A(2),angedahuricoside B(3),isofraxidin-7-O-β-d-glucopyranoside(4),fraxidin-8-O-β-d-glucopyranoside(5),(-)-marmesinin(6),(2 S,3 R)-3-hydroxymarmesinin(7),hyuganoside V(8),daucosterol(9),and sucrose(10).Conclusion: Compounds 1 –3 are new ones and compounds 4 –6 and 8 are obtained from title plant for the first time.     

对氧磷酶基因多态性与脑梗死关联性的探讨

目的:探讨我国上海地区汉族人群的对氧磷酶1(PON1)基因T-107C、PON2 Ser311Cys基因多态性与脑梗死发病乃至颈动脉硬化之间的关系。方法:采用聚合酶链反应-限制性片段长度多态性技术,检测134例脑梗死患者和103名健康对照者PON1 T-107C、PON2 Ser311Cys的多态性基因型,并研究其对脑梗死发病的影响。结果:多因素多元逐步Logistic回归分析显示,三酰甘油、总胆固醇是脑梗死的独立危险因素;同时,脑梗死组的PON1 T-107C、PON2 Ser311Cys基因型及等位基因频率分布与健康对照组间差异均有统计学意义(P     

粉尘螨在空气净化器作用下扫描电镜形态观察

目的用扫描电镜观察粉尘螨经空气净化器等离子静电高压场强作用后其形态学变化,为除螨空气净化器的研制提供依据。方法取粉尘螨成虫放置在空气净化器中,按不同时间段(0、12、24、48、72和84h)分组,每组200只粉尘螨,取出后于解剖显微镜下观察粉尘螨的死亡状况;用扫描电镜观察实验前粉尘螨和死亡粉尘螨的形态学变化,用扫描电镜进行扫描、拍照。结果用扫描电镜能清楚地观察到粉尘螨颚体、躯体、足和外生殖器的超微结构。经空气净化器高压场强作用死亡后的粉尘螨,整个躯体呈现干扁皱缩状,四肢向躯体卷曲,表面刚毛凌乱,外形明显改变,雌、雄外生殖器变形,结构不清晰。结论当空气净化器将空气中的大量水分电离成为离子状态时,造成局部相对干燥的环境而使粉尘螨脱水死亡,故空气净化器具有杀灭粉尘螨的作用。     

The branching pattern of villous capillaries and structural changes of placental terminal villi in type 1 diabetes mellitus

Maternal diabetes is associated with changes of the placental structure. These changes include great variability of vascularity manifested by strikingly hypovascular as well as hypervascular terminal villi. In this paper, normal placental terminal villi and pathological villi of type 1 diabetic placentas were compared concerning the structure of villous stroma, spatial arrangement of villous capillary bed and quantitative assessment of capillary branching pattern. Formalin fixed and paraffin embedded specimens of 14 normal and 17 Type 1 diabetic term placentas were used for picrosirius staining, vimentin and desmin immunohistochemistry and confocal microscopy. 3D models of villi and villous capillaries were constructed from stacks of confocal optical sections. Hypervascular as well as hypovascular villi of diabetic placenta displayed changed structure of villous stroma, i.e. the collagen envelope around capillaries looked thinner and the network of collagen fibers seemed less dense. The desmin immunocytochemistry has shown that stromal cells of hypervascular as well as hypovascular villi appeared nearly or completely void of desmin filaments. In comparison with normal villi, capillaries of hypovascular villi had a smaller diameter and displayed a markedly wavy course whereas in hypervascular villi numerous capillaries occurred in reduced stroma and often had a large diameter. The quantitative assessment of capillary branching has shown that villous capillaries are more branched in diabetic placentas. It is concluded that type 1 maternal diabetes enhances the surface area of the capillary wall by elongation, enlargement of diameter and higher branching of villous capillaries and disrupts the stromal structure of terminal villi.     

ICU综合征的影响因素分析

目的 探讨影响ICU综合征发生的因素,以制定有效的护理对策.方法 收集2007年6-9月入住ICU的危重症患者的资料,包括年龄、性别、文化程度、宗教信仰、家庭收入、医疗费报销情况、是否有家庭成员缺失、原发病、患病时间、性格类型、急性生理及慢性健康评分Ⅱ等,采用SPSS 11.0统计软件包处理,单因素分析采用X2检验,多因素分析采用Logistic回归分析.结果 本组进入研究患者201例,其中54例发生ICU综合征,发生率26.9%;ICU综合征发生的影响因素为是否使用呼吸机治疗、住ICU时间、睡眠情况、性别、患病时间和是否经历同室患者死亡.结论 ICU综合征发病率较高,并受多种因素影响.有针对性地采取护理措施,如加强与患者交流,重视患者家属对患者的情感支持等,有助于降低ICU综合征发生率.