颌骨骨肉瘤细胞核形态测量和DNA定量研究

为研究不同组织类型及分化程度的骨肉瘤在细胞核形态及ＤＮＡ含量方面的差异，用多功能图像分析仪对４７例颌骨骨肉瘤进行了细胞核形态测量和ＤＮＡ定量研究。结果发现，随着肿瘤恶性程度的增加，骨母细胞型和软骨母细胞型骨肉瘤的细胞核面积增大，形态更加不规则；纤维母细胞型骨肉瘤的核面积则逐渐变小，细胞核趋于圆形。肿瘤的ＤＮＡ含量和大于５倍体（＞５ｃ）瘤细胞的数量随其恶性程度的增加而增多，近２．５ｃ瘤细胞的数量则逐渐减少。３１例３年随访结果表明，肿瘤ＤＮＡ含量的平均值、大于５ｃ体细胞的数量以及肿瘤的倍体水平是判断颌骨骨肉瘤预后的重要指标。     

Role of Surgical Margin on Local Recurrence in High Risk Extremity Osteosarcoma: A Case-Controlled Study

Background

The relationship between surgical margin and local recurrence (LR) in osteosarcoma patients with poor responses to chemotherapy is unclear. Moreover, the incidences of LR according to three different resection planes (bone, soft tissue, and perineurovascular) are not commonly known.

Methods

We evaluated the incidence of LR in three areas. To assess whether there is a role of surgical margin on LR in patients resistant to preoperative chemotherapy, we designed a case (35 patients with LR) and control (70 patients without LR) study. Controls were matched for age, location, initial tumor volume, and tumor volume change during preoperative chemotherapy.

Results

LR occurred at the soft tissues in 18 cases (51.4%), at the perineurovascular tissues in 11 cases (31.4%), and at the bones in six cases (17.2%). The proportion of inadequate perineurovascular margin was higher in the case group than in the control group (p = 0.01). Within case-control group (105 patients), a correlation between each margin status and LR at corresponding area was found in the bone (p < 0.001) and perineurovascular area (p = 0.001).

Conclusions

LR is most common in soft tissues. In patients showing similar unfavorable responses to chemotherapy, the losses of perineurovascular fat plane on preoperative magnetic resonance imaging may be a valuable finding in predicting LR.     

稳定表达TSLC1基因骨肉瘤MG63细胞系的建立及鉴定

目的 建立稳定表达人肺癌抑癌基因-1（TSLC1）骨肉瘤细胞系并对其进行鉴定.方法 脂质体转染的方法 将真核表达载体pCI-TSLC1稳定转染至MG63细胞系,经克隆化培养以及G418筛选,获得了1株稳定高表达TSLC1蛋白的细胞系.对其表达的蛋白性质进行了鉴定,对其分泌动态、细胞纯度、遗传稳定性、外源基因整合等生物学特性做了鉴定.结果 获得高表达TSLC1的稳定细胞系,命名为M8T.生物学性状研究结果 表明该转基因细胞系纯度好,遗传性状稳定,抽提细胞RNA进RT-PCR,扩增到与预期结果 大小一致的DNA片段,说明TSLC1基因成功整合到细胞基因组中.细胞系无细菌和霉菌污染.结论 成功建立了稳定表达TSLC1的骨肉瘤细胞系M8T.该细胞系遗传性质稳定,为进一步研究抑癌基因TSLC1在骨肉瘤中的作用奠定了基础.     

Impact of family structure on long-term survivors of osteosarcoma

Goals of work Long-term outcomes of osteosarcoma have dramatically improved with the use of modern combination therapies. Such aggressive treatments, however, entail chronic complications. In the present study, we assessed the functional, psychological, and familial status of long-term survivors of osteosarcoma treated at our institution. Materials and methods Fifteen long-term survivors of osteosarcoma were evaluated for functional and psychological sequelae. Functional assessment was based on a method described by Enneking et al. Psychological assessment was based on General Health Questionnaire 28, Inventory Scale for Traumatic Neurosis, and Family System Test. Main results Ten patients showed mild functional impairments; only five patients were handicapped more seriously. Depressive symptoms were diagnosed in four patients. A total of six patients revealed unbalanced family structures, including three of the four patients with depressive symptoms, all four patients with symptoms of posttraumatic stress disorder, and five of seven patients who showed poor emotional acceptance. Conclusions Osteosarcoma survivors will generally recover good functional performance. Only a minority of them remain seriously impaired. One third of the patients present depressive symptoms and posttraumatic stress disorder. Poor coping is closely associated with unbalanced family structures. Therefore, the psychological and familial situation of patients with newly diagnosed osteosarcoma should be carefully assessed.     

CIK/NK细胞对荷骨肉瘤NOD/SCID小鼠肺转移瘤的杀伤作用

目的制备骨肉瘤U-2OS细胞NOD/SCID小鼠肺转移模型,探讨CIK/NK细胞对肺转移瘤的杀伤作用。方法 60只NOD/SCID小鼠随机分为3组(每组20只),A组:每只鼠经尾静脉接种U-2OS细胞2×106(0.2 ml)后24 h,输注CIK/NK细胞(2×107/0.2 ml);B组:每只鼠经尾静脉接种U-2OS细胞2×106(0.2 ml)后24 h,注射生理盐水0.2 ml;C组:每只鼠经尾静脉注射生理盐水0.2 ml后24 h,注射生理盐水0.2 ml。比较各组小鼠生存时间及肺部肿瘤负荷情况。结果 B组接种U-2OS细胞后29 d内小鼠全部死亡,其中16只肺部出现肉眼可见瘤块;A组接种U-2OS细胞后60 d内小鼠死亡8只,肺部均发现肉眼可见瘤块,余12只肺部均无瘤块。A组平均生存时间显著高于B组,差异有统计学意义(P<0.05),而与C组比较差异无统计学意义(P>0.05)。B组小鼠死亡时体重明显低于A组,而肿瘤细胞比例明显高于A组(P<0.05)。结论 CIK/NK细胞在NOD/SCID小鼠体内有肯定的抗骨肉瘤肺转移瘤效果。     

毛细血管扩张性骨肉瘤的影像学分析

目的探讨研究毛细血管扩张性骨肉瘤的影像学特点与临床的关系.方法分析16例毛细血管扩张性骨肉瘤的临床及影像学特点.其中16例中均摄X线片,13例做CT扫描检查,8例做MR检查.结果 16例肿瘤均发生长管状骨.其中位于胫骨近端的6例,肱骨近端6例,3例位于股骨远端,胫骨远端的1例.X线及CT、MR均可见溶骨性破坏及囊状膨胀性改变.肿瘤破坏区可见软组织肿块影及囊状液平.骨皮质变薄及筛孔样破坏.结论毛细血管扩张性骨肉瘤的影像学表现及临床病理上有一定的特点.对疾病的诊断和治疗提供了依据.     

A curative treatment strategy using tumor debulking surgery combined with immune checkpoint inhibitors for advanced pediatric solid tumors: An in vivo study using a murine model of osteosarcoma

Background/purpose

This study aimed to assess the significance of tumor debulking surgery by using immune checkpoint inhibitors for advanced pediatric solid tumors in a murine model of advanced osteosarcoma.

太赫兹波对MG-63荷瘤小鼠快速活体成像的实验研究

摘要 [目的]实验研究太赫兹波对人成骨肉瘤细胞株MG-63荷瘤小鼠活体体内恶性肿瘤组织能否显像及其特异性,并探讨太赫兹波技术在骨肉瘤肿瘤组织切除术中的指导作用。[方法]利用华中科技大学光电国家重点实验室连续太赫兹成像系统,进行人成骨肉瘤细胞株MG-63荷瘤小鼠活体的THz波显像,并在活体成像指导下进行瘤体切除术（实验组）与肉眼下行瘤体切除术（对照组）再次检测进行对比,术中取出组织送病理切片;将术后人成骨肉瘤MG-63荷瘤小鼠继续饲养一周后再次行THz波显像。[结果]活体人成骨肉瘤MG-63荷瘤小鼠体内瘤体组织处出现强烈吸收信号,与周围组织差异明显;术后THz波显像及病理切片均显示太赫兹波指导下行瘤体切除术比肉眼下行瘤体切除术更为彻底;继续饲养一周后对照组荷瘤小鼠原位再次复发。[结论]太赫兹波技术能对人成骨肉瘤细胞株MG-63荷瘤小鼠中肿瘤组织特异性显像,且能良好指导手术切除恶性肿瘤组织,有望在临床中应用。     

骨肉瘤影像组学的研究现状与进展

影像组学从常规影像学图像中提取肉眼不可见的定量成像特征进行数据分析和模型构建来帮助临床决策,可弥补常规影像分析的不足,可能对将来骨肉瘤的精准诊治具有重要指导意义。本文简要介绍骨肉瘤影像组学的工作流程,回顾影像组学在骨肉瘤中的最新应用,并讨论骨肉瘤影像组学的局限性和未来的研究方向。     

miR-155在骨肉瘤发生发展及肺转移进程中的作用及机制

目的 初步探究miR-155在骨肉瘤发生发展及其肺转移进程中的作用,并探讨其靶蛋白及相关机制。方法 通过数据库分析,结合临床组织与细胞样本的相关检测,对骨肉瘤及其肺转移进程中miR-155差异性进行对比分析;应用iTRAQ定量蛋白质组学技术,筛选骨肉瘤及其肺转移进程中miR-155的靶蛋白;在临床组织与细胞样本中,对筛选的miR-155靶蛋白进行验证。结果 miR-155在骨肉瘤组织和肺转移瘤组织中,较对照组均出现明显升高;与人正常成骨细胞相比,人骨肉瘤细胞系miR-155亦出现明显升高。利用iTRAQ实验得到3 714个蛋白,筛选出差异表达蛋白253个,其中上调144个,下调109个。经预测分析和实验验证C/EBP β是miR-155潜在的作用靶蛋白。结论 miR-155在骨肉瘤及其肺转移进程中出现显著升高,C/EBP β是其潜在的作用靶点。