Identification of a S100 calcium-binding protein expressed in HL-60 cells treated with all-trans retinoic acid by two-dimensional electrophoresis and mass spectrometry

To characterize the alteration of protein expression during tumor cell differentiation induced by all-trans retinoic acid (ATRA) and to understand downstream signaling and molecular mechanism of ATRA action, we compared the protein expression profiles in HL-60 cells with ATRA treatment using two-dimensional electrophoresis (2-DE). Although many changes in protein expression were found in 2-DE maps, here we identified two protein spots remarkably expressed in the differentiated cells by nanoelectrospray ionization mass spectrometry and database searching. These two protein spots were found to be the same protein, namely S100 calcium-binding protein A9 (S100A9). Further study will be done to ascertain whether S100A9 plays a role in the regulation of differentiation or just a consequence of differentiation.     

黑色素瘤抗原MAGE-B亚家族基因在肝细胞癌中的表达

目的　检测黑色素瘤抗原MAGE B基因 (MAGE B1,MAGE B2 )在肝细胞癌 (HCC)中的表达 ,以期找到可用于HCC免疫治疗的新靶位。方法　用逆转录 聚合酶链反应 (RT PCR)方法检测MAGE B1、B2、A1和A3在 47例HCC患者癌组织和相应癌旁组织、3 0例肝硬化和正常肝组织中的表达 ,随机选择 4例RT PCR阳性扩增产物直接进行DNA序列测定 ,并将其表达结果与临床指标之间的关系进行分析。结果　 47例HCC组织中 ,MAGE B1和MAGE B2基因阳性率分别为 44.7% (2 1/47)和61.7% (2 9/47) ,而相应癌旁组织、肝硬化组织和正常肝组织中均为阴性。测序结果证实 ,RT PCR产物确为这两种基因。MAGE A1和MAGE A3的阳性率分别为 74.5% (3 5/47)和 44.7% (2 1/47)。MAGE B和MAGE A的表达之间有显著相关性 (P <0 .0 5) ,但其中 12例不表达MAGE A1和 (或 )A3的病例中 ,有 5例表达MAGE B1和 (或 )B2。同时检测这 4种基因的表达 ,发现至少表达其中 1种、2种、3种和4种均表达者分别为 83 .0 % (3 9/47)、55.3 % (2 6/47)、48.9% (2 3 /47)和 3 8.3 % (18/47)。MAGE B基因的表达与年龄、性别、肿瘤大小、分化程度、血清甲胎球蛋白 (AFP)水平、HBV和HCV感染之间无显著相关性 (P >0 .0 5)。结论　MAGE B基因家族在HCC中呈相对高比例、高特异表达 ,可     

Promoting of melanocyte adhesion and migration by Malytea Scurfpea fruit in vitro

The aim of this study was to investigate the effect of Malytea Scurfpea Fruit (MSF) on melanocyte adhesion and migration. Human epidermal melanocytes were treated with MSF and examined for adhesion to bovine serum fibronectin-coated culture dishes. Control and treated cells were also examined for migration into micropore filters coated with the same protein. Compared with control, MSF-treated melanocytes adhered to the dishes more easily and migrated into the filters in a dose-dependent manner. MSF at a dose of more than 200 micro g/ml did not increase melanocyte adhesion and migration accordingly. With the exception of MSF 10 micro g/ml, at every concentration of MSF there were significant differences between treated and untreated melanocytes (p < 0.01) when the adhesion test was studied. Regarding migration, even at a concentration of MSF 10 micro g/ml, obviously increased cell numbers were found compared with MSF untreated melanocytes (p < 0.01). MSF promoted melanocyte adhesion and migration; this could explain, in part, the capacity of MSF to regulate melanocyte function in vitiligo.     

The pontic-splinted procedure for tooth and denture base additions in denture repair

Various methods for adding teeth to existing dentures have been used clinically. Although a direct, single-tooth addition method saves time, it often is difficult to achieve an esthetic appearance when adding several missing anterior teeth with this technique. Indirectly adding teeth may achieve a better esthetic result, but this method is time-consuming. This article describes the pontic-splinted procedure, an indirect method for replacing several anterior teeth and part of the denture base that can save time and, at the same time, help the clinician achieve acceptable esthetics.     

Detection of viable myocardium by low dose of dobutamine cine MR imaging in miniswine

Objective To evaluate the diagnostic value of dobutamine stress magnetic resonance imaging (MRI) for myocardial viability.Methods Ten male miniswines underwent left ventriculography and coronary angiography, followed by stenosis of the left circumflex coronary artery (LCX) using ameroid constrictor. More than one month later, left ventriculography and coronary angiography were performed again, followed by cine-MRI at rest and during stress with incremental dose of dobutamine 5-20 μg·kg-1·min-1. Traditional and/or breath-hold cine-MRI were used to evaluate regional left ventricular wall motion, corresponding to basal, midventricular and apical short-axis tomograms. Regional wall motion score index (WMSI) was calculated.The miniswines were finally sacrificed for pathological examination. Triphenyl tetrazolium chloride (TTC) delineated myocardial infarction. Microscopy was used to identify myocardial cellular changes. Results One pig died, one pig suffered from aneurysm and another showed no negative findings. The other seven pigs were found with hypokinetic (n=4) or akinetic (n=3) myocardial regions related to stenosed LCX. Their mean WMSI at rest for the lateral and posteroinferior walls (ischemic regions) of the left ventricle was 2.27±0.32, as compared with 1.00±0.00 (P&lt;0.01) for the corresponding nonischemic anteroseptal regions. Further, the mean WMSI for the ischemic regions was 2.27±0.32 at rest compared with 1.40±0.39(P&lt;0.01) at the dose of dobutamine 5 μg·kg-1·min-1. However, the mean WMSI at the doses of dobutamine 10 and 20 μg·kg-1· min-1 were 1.70±0.76 and 1.75±0.83, respectively, with no significant difference as compared with the mean WSCI at rest (P&gt;0.05). The pathologic examination showed viable myocardium at the ischemic regions. Conclusion Low-dose dobutamine (5 μg·kg-1·min-1) recovers hypokinetic or akinetic myocardial regions, and dobutamine stress MRI can be used to detect myocardial viability.     

瘦素基因启动子区-2548G/A功能多态性与抗精神病药源性肥胖的相关分析

目的　探讨瘦素基因启动子区 - 2 5 4 8G/A功能多态性是否与首次治疗精神分裂症患者的抗精神病药物 (APS)急性期治疗导致的体重增加相关。方法　采用聚合酶链反应 限制片段长度多态性 (PCR RFLP)技术分析 12 8例患者 (男 6 1例 ,女 6 7例 ) - 2 5 4 8G/A等位基因和基因型分布频率 ,APS(利培酮或氯丙嗪 )单药治疗 10周 ,治疗前后每周测体重和体重指数 (BMI) ,采用MRI测治疗前后腹部脂肪分布 (30例 ) ,分析等位基因和基因型与基础体重指标和其变化值的相关性 ;同时分析 38名性别、年龄和BMI相匹配的健康对照者 ,其中 2 2例进行MRI扫描。结果　治疗后患者体重增加是基础体重的 (6 .2± 5 .7) % ,腹部脂肪增加是基础腹部皮下脂肪 (SUB)的 (38.5± 4 2 ) %、腹内脂肪的 (4 0 .0± 4 1.2 ) % ;- 2 5 4 8G/A等位基因和基因型在患者组和对照组分布频率差异无显著意义 ;在体重增加≤ 7%和 >7%患者组等位基因和基因型分布频率差异有非常显著意义 (χ2 =7 5 2 9,df =1,P =0 .0 0 6 ;OR =1.94 1;95 %CI:1.175～ 3.2 0 7) ;基因型对患者组或对照组的基础体重指标无显著影响 ;与携带G等位基因患者相比 ,AA基因型携带者治疗后BMI(P =0 .0 0 3)和SUB(P =0 .0 0 9)明显增加。结论　瘦素基因启动子区 - 2 5 4 8G/A功能多态     

干扰素治疗病毒性肝炎白细胞下降的临床观察

目的探讨干扰素治疗慢性病毒性肝炎过程中出现的白细胞下降的趋势及相应对策。方法监测8例慢性病毒性肝炎患者在普通干扰素3～5MU[通用名:重组人干扰素α-2b,商品名:凯因益生(配预充注射器),由北京凯因生物技术有限公司出品]抗病毒治疗过程中出现的白细胞下降,予以G-CSF治疗4周。结果干扰素抗病毒治疗1周～2周即可出现白细胞下降,加用G-CSF治疗后均可达到使用干扰素指征。结论干扰素治疗慢性病毒性肝炎过程中出现的白细胞下降并非骨髓抑制,加用G-CSF治疗后均可达到使用干扰素指征。     

微创技术在腹腔镜胆囊切除术后黄疸诊治中的应用

目的：探讨微创技术在腹腔镜胆囊切除术（LC）后黄疸的诊断与治疗中的作用。方法：在微创观念指导下，对LC术后出现黄疸的患者运用内镜逆行胰胆管造影（ERCP）和内镜括约肌切开（EST）等微创技术进行诊断与治疗。结果：我院1998年8月至2001年5月间共行3160例LC手术，术后出现黄疸者5例，均先接受B超、ERCP检查，提示胆总管结石与胆总管末端狭窄，3例EST取石一次成功，1例首次EST取石失败后予保守治疗5天后再行ERCP与碎石治疗成功，1例因结石较多较大行开腹胆总管探查术。结论：对LC术后黄疸，在B超等检查排除明显胆道损伤和胆漏后，胆总管结石应首先考虑。ERCP可进一步明确诊断，EST取石或碎石这一微创技术治疗胆道细小结石伴有胆总管末端炎性狭窄引起的梗阻效果良好。     

植入式给药装置两种针穿刺落屑比对试验

报告了普通注射针和Huber注射针对植入式给药装置的注射区进行了穿刺落屑比对试验,结果表明,采用Huber注射针穿刺后的落屑数均明显少于普通注射针的试验结果。建议应在我国推广用Huber针对植入式给药装置的药物输注。     

Partial Restoration of Left Ventricular Systolic Function by asPLB Gene Transfer Using Ultrasound-Mediated Microbubble Destruction

In vitro and in vivo studies have demonstrated that inhibition of phospholamban (PLB) expression in myocardium can restore left ventricular systolic function in failing heart. Ultrasound mediated microbubble destruction provides a new option for noninvasive gene transfer in heart. In this study, we transfered pAAV-antisense phospholamban (pAAV-asPLB) to the hearts of myocardial infarction (MI) mice, using ultrasound mediated microbubble destruction. Then we estimated the protein levels of PLB, Ser16-PLB and cardiac sarcoplasmic reticulum Ca²⁺ ATPase (SERCA). The left ventricular ejection fraction (LVEF), fraction shortening (FS) and SERCA activity were measured as well. MI mice were generated by ligating the left anterior descending coronary artery. Microbubbles were prepared by sonicated perfluorocarbon gas with dextrose and albumin. A mixture of pAAV-asPLB plasmid and microbubble was injected via tail vein while the heart was simultaneously exposed to ultrasound via transthoracic insonation. Three weeks later, LVEF (48.2 ± 5.18% vs 39.1 ± 5.38%, p < 0.05), FS (19.6 ± 2.59% vs 16.0 ± 2.29%, p < 0.05), SERCA activity (3.00 ± 0.29 vs 2.12 ± 0.30, p < 0.05) and Ser16-PLB protein level (0.8 ± 0.25 vs 0.46 ± 0.18, p < 0.05) were increased while PLB protein level (1.45 ± 0.38 vs 2.05 ± 0.31, p < 0.05) was decreased compared with the MI mice with saline injection. The above parameters in MI mice with only pAAV-asPLB plasmid injection or pAAV-asPLB plasmid combined with ultrasound alone were not significantly improved. pAAV-LacZ was used as a reporter gene to determine the efficiency and localization of transfection. The expression of β-galactosidase was not found in liver, lung and brain, but found only in tubular epithelial cells of kidney and found in heart. These results confirm that asPLB gene transfection can be achieved by ultrasound mediated microbubble destruction with organ specificity. The effective transfection can partly restore heart function in MI mice. (E-mail: s0hu0001@hotmail.com)