治疗性HBV DNA疫苗的研究进展

乙型肝炎病毒（HBV）的持续感染可引起肝脏疾患，甚至肝硬化和原发性肝细胞癌。近年来，HBV DNA疫苗在治疗慢性HBV携带者和慢性乙型肝炎方面越来越引起人们的重视。本文主要就治疗性HBV DNA疫苗的构成、治疗慢性乙型肝炎的依据及其研究进展进行综述。     

乙型肝炎病毒X基因突变与肝细胞癌

原发性肝细胞癌是世界上最常见的恶性肿瘤之一,慢性HBV感染是导致肝细胞癌的主要病因。HBV诱发肝细胞癌的确切机制仍不十分清楚。目前在HBV相关肝细胞癌病人血清和肝癌组织中发现了许多HBV X基因突变体,一些与肝细胞癌发生发展密切相关。本文主要就HBV X基因突变与肝细胞癌研究进展进行综述。     

脑外科病房细菌监测及分析

目的 :通过对脑外科病房环境进行细菌学分析 ,观察分析抢救病房和普通病房空气与物表细菌检出及分布情况 ,根据监测的结果 ,采取相应整改措施 ,减少医院感染。方法 :对脑外科抢救病房与普通病房的空气和物表细菌进行培养计数和鉴定其细菌数量和性质。结果 :抢救病房空气、物体表面的细菌数均高于普通病房 ,其中以凝固酶阴性葡萄球菌、不动杆菌和铜绿假单胞菌微球菌为主。结论 :抢救病房是重病监护又易出现医源性感染的单元 ,应加强抢救病房细菌监测和管理 ,改善病房环境 ,采取各种预防措施 ,防止病原菌播散 ,从而达到降低医院感染的目的。     

治疗性HBV DNA疫苗的研究进展

乙型肝炎病毒(HBV)的持续感染可引起肝脏疾患,甚至肝硬化和原发性肝细胞癌.近年来,HBV DNA疫苗在治疗慢性HBV携带者和慢性乙型肝炎方面越来越引起人们的重视.本文主要就治疗性HBV DNA疫苗的构成、治疗慢性乙型肝炎的依据及其研究进展进行综述.     

流动儿童计划免疫现状分析及对策

目的掌握深圳市盐田区流动儿童的免疫现状,从而加强流动儿童的计划免疫管理。方法按照《广东省流动儿童计划免疫状况调查方案》,于2003年11月在调查区域采取挨门逐户调查流动儿童的计划免疫接种状况。结果深圳市盐田区流动儿童的“五苗”接种率低于90%,“五苗”全程免疫覆盖率为80.70%;有6.79%的流动儿童仍存在免疫空白;且流动儿童的“五苗”接种率和全程免疫覆盖率均明显低于常住儿童。结论计划免疫管理已成为制约盐田区计划免疫工作深入开展的关键。为进一步完善深圳市计划免疫工作,健全流动人口管理制度,积极改进计划免疫的服务方式,加强规范化接种门诊的建设,提高计划免疫的服务质量,加大针对流动儿童的计划免疫宣传力度。     

乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒抑制HBsAg、HBV DNA分泌的体外实验

Objective To investigate the inhibitive activities of hepatitis B immunoglobulin(HBIG)poly(butylcynaoacrylate)nanoparticles(HBIG-PBCA-NP)to hepatitis B surface antigen(HBsAg)and hepatitis B virus(HBV)DNA secretions using HBV infected cell model in vitro.Methods HepG 2.2.15 cells were cultured with media containing HBIG-PBCA-NP or HBIG for several days,or cultured with HBIG-PBC-NP and HBIG for 2 days and without HBIG-PBCA-NP and HBIG from day 3.The supernatants at different time points were collected for quantitative detection of HBsAg and HBV DNA.The comparisons between groups were done by variance analysis.Resalts Secretions of HBsAg and HBV DNA in supernatants of HepG2.2.15 cultured with 0.1-10.0 IU/mL of HBIG-PBCA-NP and HBIG were inhibited significantly compared with control group.HBsAg titers and HBV DNA levels in supernatants of HBIG-PBCA-NP group and HBIG group cultured with media without HBIG-PBCA-NP and HBIG kept decreasing at day 5 and 7,then rebounded at day 9 and 11.HBsAg titera in supernatants of 0.1,1.0,5.0 IU/mL HBIG-PBCA-NP group were all significantly different from those in HBIG group at day 9[(31.31±1.98)μg/L vs(40.62±2.99)μg/L,(23.79±1.31)μg/L vs(36.51±2.12)μg/L,(19.91±1.74)μg/L vs(33.03±1.65)μg/L;F=412.24,P＜0.01].Couclusion HBIG-PBCA-NP can inhibit secretions of HgsAg and HBV DNA in vitro,which is more effective than HBIG.     

乙型肝炎免疫球蛋白聚氰基丙烯酸正丁酯纳米粒抑制HBsAg、HBV DNA分泌的体外实验

Objective To investigate the inhibitive activities of hepatitis B immunoglobulin(HBIG)poly(butylcynaoacrylate)nanoparticles(HBIG-PBCA-NP)to hepatitis B surface antigen(HBsAg)and hepatitis B virus(HBV)DNA secretions using HBV infected cell model in vitro.Methods HepG 2.2.15 cells were cultured with media containing HBIG-PBCA-NP or HBIG for several days,or cultured with HBIG-PBC-NP and HBIG for 2 days and without HBIG-PBCA-NP and HBIG from day 3.The supernatants at different time points were collected for quantitative detection of HBsAg and HBV DNA.The comparisons between groups were done by variance analysis.Resalts Secretions of HBsAg and HBV DNA in supernatants of HepG2.2.15 cultured with 0.1-10.0 IU/mL of HBIG-PBCA-NP and HBIG were inhibited significantly compared with control group.HBsAg titers and HBV DNA levels in supernatants of HBIG-PBCA-NP group and HBIG group cultured with media without HBIG-PBCA-NP and HBIG kept decreasing at day 5 and 7,then rebounded at day 9 and 11.HBsAg titera in supernatants of 0.1,1.0,5.0 IU/mL HBIG-PBCA-NP group were all significantly different from those in HBIG group at day 9[(31.31±1.98)μg/L vs(40.62±2.99)μg/L,(23.79±1.31)μg/L vs(36.51±2.12)μg/L,(19.91±1.74)μg/L vs(33.03±1.65)μg/L;F=412.24,P＜0.01].Couclusion HBIG-PBCA-NP can inhibit secretions of HgsAg and HBV DNA in vitro,which is more effective than HBIG.     

肝细胞癌组织中HBx基因缺失型突变体真核表达载体的构建和鉴定

目的 构建缺失型突变体HBx-d382和HBx-d431真核表达载体.方法 以pGM-T/HBx-d382和pGM-T/HBx-d431质粒为模板,PCR扩增含Kpn Ⅰ和Apa Ⅰ酶切位点的HBx基因片段.双酶切后再与载体pcDNA3载体重组连接.重组质粒转化到大肠杆菌DH5a后,经酶切鉴定和DNA序列测定,筛选出重组pcDNA3/HBx-d382和pcDNA3/HBx-d431真核表达载体.结果 成功构建了重组pcDNA3/HBx-d382和pcDNA3/HBx-d431真核表达载体,经DNA序列测定与比对,重组前后HBx基因片段序列一致.结论 pcDNA3/HBx-d382和pcDNA3/HBx-d431真核表达载体构建成功,可为HBx基因缺失型突变体,特别是HBx-d382基因的生物学功能研究提供基因材料.     

联合测定心型脂肪酸结合蛋白、肌钙蛋白T诊断早期急性心肌损伤

目的:联合测定心型脂肪酸结合蛋白(h-FABP)、肌钙蛋白T(cTnT)对早期诊断急性心肌损伤的意义.方法:对76例急性冠脉综合征(ACS)患者,自胸痛发生后1 h开始每小时连续监测心型脂肪酸结合蛋白、肌钙蛋白T至胸痛发生后6 h,共6次.结果:在早期急性心肌损伤中,h-FABP阳性结果出现的时间早于cTnT,二者对比有统计学意义(P＜0.05).在cTnT升高患者中,h-FABP升高率为98.7%,二者对比无统计学差异(P＞0.05).结论:在诊断早期急性心肌损伤时,h-FABP出现阳性结果时间早于cTnT,而联合测定h-FABP、cTnT对提高ACS患者早期确诊率,指导临床急救具有重要的价值.     

HBx基因缺失型突变体QSG7701细胞转染模型的建立与鉴定

目的 建立稳定表达乙型肝炎病毒X基因缺失型突变体HBx-d382和HBx-d431蛋白QSG7701肝细胞株.方法 HBxd382和HBx-d431基因经PCR和双酶切后,插入到真核表达载体pcDNA3中,构成重组质粒peDNA3/HBx-d382和pcDNA3/HBx-d431.应用基因转染的方法,将重组质粒分别转染到QSG7701肝细胞中,经G418筛选和RT-PCR、蛋白印迹鉴定,筛选出稳定表达HBx蛋白的细胞株.结果 经PCR、酶切及序列鉴定,重组质粒pcDNA3/HBx-d382和pcDNA3/HBx-d431构建成功.RT-PCR和Western blot结果湿示,该重组质粒能在QSG7701肝细胞中表达插入的HBx-d382和HBx-d431基因编码的融合蛋白.结论 成功建立了稳定表达羧基端截短的HBx蛋白肝细胞株HBx-d382和HBx-d431.HBx-d382和HBx-d431肝细胞模型的建立,为HBx-d382和HBx-d431基因及其蛋白的深入研究奠定了基础.