全文获取类型
收费全文 | 7690篇 |
免费 | 518篇 |
国内免费 | 121篇 |
专业分类
耳鼻咽喉 | 150篇 |
儿科学 | 90篇 |
妇产科学 | 107篇 |
基础医学 | 531篇 |
口腔科学 | 123篇 |
临床医学 | 783篇 |
内科学 | 657篇 |
皮肤病学 | 147篇 |
神经病学 | 263篇 |
特种医学 | 471篇 |
外科学 | 795篇 |
综合类 | 1296篇 |
预防医学 | 496篇 |
眼科学 | 49篇 |
药学 | 502篇 |
5篇 | |
中国医学 | 210篇 |
肿瘤学 | 1654篇 |
出版年
2024年 | 4篇 |
2023年 | 68篇 |
2022年 | 188篇 |
2021年 | 228篇 |
2020年 | 213篇 |
2019年 | 172篇 |
2018年 | 173篇 |
2017年 | 218篇 |
2016年 | 212篇 |
2015年 | 222篇 |
2014年 | 480篇 |
2013年 | 417篇 |
2012年 | 438篇 |
2011年 | 531篇 |
2010年 | 431篇 |
2009年 | 393篇 |
2008年 | 414篇 |
2007年 | 413篇 |
2006年 | 342篇 |
2005年 | 302篇 |
2004年 | 242篇 |
2003年 | 216篇 |
2002年 | 201篇 |
2001年 | 189篇 |
2000年 | 152篇 |
1999年 | 151篇 |
1998年 | 138篇 |
1997年 | 116篇 |
1996年 | 124篇 |
1995年 | 133篇 |
1994年 | 97篇 |
1993年 | 92篇 |
1992年 | 58篇 |
1991年 | 63篇 |
1990年 | 63篇 |
1989年 | 53篇 |
1988年 | 47篇 |
1987年 | 39篇 |
1986年 | 27篇 |
1985年 | 41篇 |
1984年 | 32篇 |
1983年 | 14篇 |
1982年 | 31篇 |
1981年 | 35篇 |
1980年 | 29篇 |
1979年 | 23篇 |
1978年 | 11篇 |
1977年 | 6篇 |
1976年 | 13篇 |
1972年 | 27篇 |
排序方式: 共有8329条查询结果,搜索用时 203 毫秒
41.
Human glioma cells obtained from established cell lines (Tp-276MG, Tp-301MG, Tp-378MG, Tp-483MG and U-251MG) were analyzed for the presence of ion channels with the tight-seal voltage clamp technique. The current-voltage relation revealed a marked inward rectification at hyperpolarizing voltages, due to the presence of inward rectifying K-channels in cells from all studied cell lines. These channels were conducting when the membrane potential was more negative than the K-equilibrium potential. The slope conductance for the inward K-currents (gKi) was affected both by [K+]i and [K+]0. gKi was proportional to [K+]0 raised to 0.35 or 0.50, of which the larger value was measured in the presence of low [K+]i (25mM). The rectification was not significantly different in cells perfused with Mg-free EDTA-buffered internal solution. Tl+ was 3.5 times more permaant than K+. gki was blocked by Cs+ (1 mM) in a voltage-dependent way (more effective in the hyperpolarized membrane), and by Na+ (154 mM) depending on voltage and time. From measurements of unitary current events in membrane patches (outside out or cell attached) the conductance of the single inward rectifying channel was estimated to be 27 ± 7 pS. This type of ion channel may be important for K-uptake by glial cells and hence for the K-homeostasis in the brain. 相似文献
42.
C. Constanzo I. T. Jackson C. McEwan J. M. Self 《European journal of plastic surgery》1987,9(4):137-140
Summary The clinical features and histological appearance of desmoplastic malignant melanoma are presented. Aggressive surgical management and close follow-up are mandatory if this highly aggressive tumor is to be controlled. Despite this, the prognosis is poor. 相似文献
43.
目的:了解恶性淋巴瘤(ML)发病、病理及免疫分型特点,探讨基因分型在诊断中的作用。方法:通过标准链菌素生物素-过氧化物酶标记物法(SABC法)对病理标本进行免疫分型,PCR检测病理及骨髓标本IgH(FR2A,3A)和TCR(β,γ)基因重排,同时对临床及病理资料进行多因素分析。结果:(1)ML非霍奇金淋巴瘤(NHL)较霍奇金淋巴瘤(HL)发病率高,发病率随年龄增长而递增,60岁以上发病者占38.6%;其平均生存时间明显低于60岁以下者。(2)B-NHL发病率为68.6%,T-NHL为28.6%;B-NHL的3年生存率高于T-NHL。(3)低度恶性NHL占42%,中、高度恶性占58%;低度恶性组平均生存时间较中、高度恶性组长,但统计学上差异无显著性。生存期分析显示I-Ⅱ期NHL预后明显优于Ⅲ-Ⅳ期。(4)PCR检测病理及骨髓标本的IgH和TCR重排,B-NHL FR2A的阳性率分别为66.7%及56.2%;FR3A阳性率分别为90.4%及81.2%;T-NHL中TCRβ、γ阳性率分别为91.7%及75.0%;病理标本的阳性率略高于骨髓,T、B分型与免疫分型相符。结论:年龄,T、B分型和临床分期是影响NHL预后的重要因素;分子生物学检测作为辅助手段可以肯定免疫分型结果并补充其不足,骨髓及外周血检测除协助分型外可用于肿瘤微小残留病的监测。 相似文献
44.
Background: Percutaneous closed needle biopsy of musculoskeletal neoplasms has gained in popularity. However, it remains controversial
whether or not to resect the needle tract for fear of a local recurrence. A single published case report exists, noting the
lone tract recurrence of an extremity skeletal osteosarcoma.
Methods: We report on three additional individuals who demonstrated that tract local recurrences may occur after a closed needle biopsy
for nonosteosarcoma, nonextremity sarcomas. For perspective, the world literature is reviewed to identify tract recurrences
for other malignancies and the results of needle biopsy in musculoskeletal neoplasms.
Results: Eighty-nine percent of needle tract local recurrences occur when carcinomas are subjected to biopsy, as reported in the literature.
Forty-seven cases since 1950 are described representing essentially all tumor types. The nature of musculoskeletal neoplasms
makes closed biopsy more difficult than for softer, more homogeneous, and easier to access neoplasms.
Conclusions: Local recurrences of sarcoma may occur in closed needle biopsy tracts. Strong consideration should be given to open biopsy
and tract resection. 相似文献
45.
Summary We analysed long-term follow-up results of 175 patients with malignant glioma (110 glioblastoma and 65 anaplastic astrocytoma) treated under five different regimes during the past two decades. The factors of age (less than 40), histology (anaplastic astrocytoma) and type of adjuvant therapy (radiation and chemotherapy) contributed to long survival. The other important factor was the response to adjuvant therapy.Cases of gross total removal or complete response (CR) of a residual tumour to an adjuvant therapy showed a better prognosis. The three and five year survival rate was 42% and 24%, respectively. The highest CR ratio (23%) was seen in patients treated by intravenous injection of interferon and ACNU in addition to radiotherapy (IAR therapy). 相似文献
46.
Koibuchi Yukio; Iino Yuichi; Joshita Takashi; Yokoe Takao; Shinkai Hiroko; Kawashima Kenji; Kobayash Junya; Tanaka Sunao; Oyama Tetsunari; Hikino Toshiaki; Morishita Yasuo 《Japanese journal of clinical oncology》1995,25(6):273-277
A 66-year-old woman time of 10 days. One month after radicalmastectomy, there was local recurrence, followed by multiplepulmonary metastases, and the patient died of respiratory failure5 months after surgery. The gray-white-colored tumor measured13x12x;10 cm, and its border was well defined. The tumor wascomposed of diffusely growing round or polygonal cells withvesicular nuclei, prominent nucleoli, and ample cytoplasm containingeosinophilic inclusions. Lymph node involvement was widespread.Both vimentin and keratin were clearly demonstrated by immunohistochemicalstaining. Ultrastructural studies revealed that the MRT cellscontained cytoplasmic whorls of intermediate filaments. 相似文献
47.
48.
49.
Detection of Epstein Barr virus in an hepatic leiomyomatous neoplasm in an adult human immunodeficiency virus 1-infected patient 总被引:5,自引:0,他引:5
S. Prévot J. Néris P. P. de Saint Maur 《Virchows Archiv : an international journal of pathology》1994,425(3):321-325
We report the first case of a human immunodeficiency virus (HIV)-related primary hepatic leiomyoma in an adult patient. The diagnosis was made at autopsy and confirmed by immunohistochemistry. Epstein Barr virus (EBV) was identified in tumour cells by in situ hybridization. Review of the literature revealed 13 cases of visceral myogenic tumours occuring in acquired immunodeficiency syndrome children, and only 2 cases in adults. One was a spinal epidural leiomyoma, the other multiple smooth muscle tumours of the colon and adrenal gland. This is the first report of EBV in smooth muscle neoplastic cells in an HIV-infected adult patient. 相似文献
50.