高温致神经管畸形相关基因CDK109克隆及其致畸相关性分析

目的通过筛选高温致畸金黄地鼠胚胎神经管cDNA文库,克隆高温致畸相关基因CDK109。方法利用本室构建的高温致畸胚胎神经管cDNA文库,采用噬菌斑原位杂交方法,用寡核苷酸探针筛选cDNA文库,挑取阳性噬菌斑,将其转化为质粒,再通过限制性酶切鉴定阳性克隆并测序,将该基因标记探针与孕8.5 d高温致畸胚胎神经管组织和正常对照胚胎神经管组织总RNA进行NORTHERN杂交,以便确认该基因在高温致神经管畸形中的异常表达。结果成功地从高温致畸金黄地鼠胚胎神经管cDNA文库中筛选出高温致畸相关基因CDK109全长,其在高温致畸组的表达明显高于对照组。结论 CDK109在神经管中的高表达与高温致神经管畸形密切相关。     

Serum IgG, IgA, IgM, and IgE levels and allergy in Filipino children in the United States

The serum levels of IgE, IgG, IgA, and IgM of 27 American-born Filipino children 5 to 17 years of age were measured and found to be significantly higher than those of a control group of 24 Caucasian children of similar age distribution and attending the same general pediatric clinics. The geometric mean of serum IgE of the Filipinos was 227 U. per milliliter and of the Caucasians, 69 U. per milliliter (p < 0.01). The geometric means of other serum immunoglobulin levels of the Filipinos by comparison with the Caucasians were: IgG, 1,303 and 1,010 mg. per 100 ml. (p < 0.01); IgA, 195 and 120 mg. per 100 ml. (p < 0.001); and IgM, 141 and 92 mg. per 100 ml. (p < 0.02), respectively. The incidence of atopic disease was higher in the Filipino study group (48 per cent) than in the Caucasian control group (25 per cent); eczema was especially prevalent in the Filipino group. Elevated serum IgE levels were associated with atopic disease in both racial groups; however, there was no correlation between serum level of IgG, IgA, or IgM and atopy.     

钒化钠对食管癌细胞系EC109 Caspase-3表达的影响

目的：研究钒化钠对食管癌细胞系EC109 Caspase-3激活的影响,探讨钒化钠影响食管癌细胞系EC109细胞生长的机制。方法：以体外培养食管癌细胞系EC109为研究对象,给予0、0．1、1．0、5．0、10．0、50．0、100．0、200．0μmol／L钒化钠,培养1小时后,用Western blot技术检测食管癌细胞系EC109 Caspase-3激活情况。结果：0、0．1、1．0、5．0、10．0μmol／L钒化钠组培养1小时后,各组Caspase-3蛋白激活与对照组比较均无统计学意义;50．0、100．0、200．0μmol／L钒化钠组培养1小时后,各组Caspase-3蛋白表达均升高。结论：高浓度的钒化钠可能激活EC109细胞Caspase-3蛋白。     

靶向survivin基因的小干扰RNA对食管癌细胞Eca-109 化疗敏感性的影响

目的 利用RNAi (RNA interference, RNAi) 技术抑制食管癌Eca-109细胞survivin基因表达,观察survivin基因沉默后对Eca-109细胞化疗敏感性的影响。方法 构建靶向survivin基因特定序列的小干扰RNA(small interference RNA, siRNA)真核表达载体并转染Eca-109细胞,筛选得到稳定转染的survivin干扰组细胞Eca-109 /si-survivin,同时设转染无关干扰质粒的Eca-109细胞为阴性对照组,未转染的Eca-109细胞为空白对照组;通过Western Blot法检测干扰前后survivin基因沉默抑制效果;随后将各组细胞与不同浓度的氟尿嘧啶(5-Fu)作用,MTT法检测各组细胞对5-Fu的半数抑制浓度(IC₅₀),流式细胞仪分析各组细胞的凋亡率。结果 干扰组Eca-109 /si-survivin细胞survivin蛋白表达水平(18.75±3.12)较阴性对照组Eca-109 /si-control(44.17±3.15)及空白对照组Eca-109 (46.20±2.62)明显下调(P<0.05);联合应用5-Fu的Eca-109 /si-survivin干扰组细胞IC₅₀为(18.75±1.53)mg·L-1,显著低于空白对照组(39.65±1.75)mg·L-1和阴性对照组(38.95±1.34)mg·L-1,差异具有统计学意义(P<0.05);联合应用5-Fu的Eca-109 /si-survivin干扰组细胞凋亡率为(37.35±1.52)%,明显高于空白对照组(11.26±1.21)%和阴性对照组(12.34±1.32)%,差异具有显著性(P<0. 05)。 结论 靶向survivin的siRNA能特异性沉默survivin基因表达,抑制细胞增殖,诱导细胞凋亡,并增强人食管癌Eca-109细胞对5-Fu的化疗敏感性。     

Prediction of aortic valvular area and gradient by noninvasive techniques.

Sixty-two patients with isolated aortic valvular stenosis were analyzed by a series of common noninvasive procedures and by cardiac catheterization. The data from 50 of these were evaluated in a retrospective fashion by multiple regression methods to determine significant objectively obtained predictors of aortic-left ventricular gradient and valvular area. Formulae were derived from these analyses and an additional 12 patients were then studied prospectively to evaluate the validity of the predictive formulae. Forty-three of 50 patients (86 per cent) were correctly identified as to a gradient of greater or less than 50 mm. Hg in the initial group, and all those in the prospectively studied sample were correctly classified. Thiry-five of 43 patients (82 per cent) of those with valve area data in the first application were correctly classified as to valve area or greater or less than 0.8 cm.2, and all patients in the prospectively studied group were appropriately identified as to the same area. The combined application of the observations of calcification of the aortic valve, shudder waves on the anacrotic limb, prolonged time to peak of the percussion wave and alteration of the dicrotic notch of the carotid pulse tracing, left ventricular hypertrophy by electrocardiogram, and the altered duration of ventricular ejection time were reliable predictors of elevated aortic-left ventricular gradient and decreased aortic valvular size.     

Diabetic lipoprotein deficient serum: Its effect in low density lipoprotein (LDL) uptake and degradation by fibroblasts

Low density lipoproteins (LDL) isolated from poorly controlled diabetic patients are known to be taken up and degraded by fibroblasts at a lower rate than LDL isolated from normal subjects. This aberrant metabolic behavior has been attributed to a diabetic-related abnormality in LDL composition yet to be characterized. The studies reported in this article show that the decrease in uptake and intracellular degradation of LDL from diabetic patients is further enhanced when the cells are exposed to lipoprotein deficient serum (LPDS) isolated from the same poorly controlled diabetic patients. Comparative studies of the composition of LPDS obtained from normal donors and poorly controlled diabetic patients showed an increase in saturated and total unesterified fatty acids (UFA), lecithin, apolipoprotein A1, and immunoreactive insulin in the LPDS from diabetic patients. We postulate that exposure of cells to LPDS obtained from poorly controlled diabetic patients may induce changes in the composition of the fibroblast membrane and alter its fluidity, leading to further decrease in the uptake and degradation of LDL. During poor diabetic control, cell membrane changes, and modification of LDL composition are likely to act either additively or synergistically to induce an abnormal LDL-cell interaction. This abnormal interaction may be a relevant factor to explain the greater incidence of arteriosclerosis in diabetes mellitus.     

Studies on the antibiotic constituents of Korean basidiomycetes(IV). Preliminary examination of the mycelial cultures of the 17 basidiomycetous strains

To investigate the antibiotic constituents of Korean basidiomycetes the carpophores of the wood-rotting basidiomycetes were collected from several locations of Korea, and from them 17 mycelial strains were isolated on potato-dextrose-agar plates supplemented with tetracycline (20 μg/ml). The isolated strains were shake-cultured in glucose-peptone-yeast extract medium and then the antibacterial activities of the culture filtrates were assessed by disc-plate method. Among them, 12 strains (70.6%) were active, and basidiomycete strain LMCB-109 (Daedalea quercina) and LMCB-116 showed potent activities against all the six bacterial target organisms includingSerratia marcescens.     

Partial purification and characterization of plasma DNA and its relation to disease activity in systemic lupus erythematosus

This study reports the partial isolation and characterization of plasma DNA by phenol extraction and concentration of the extract. DNA, identified by immunologic and chemical methods, was found in plasma from both normal persons and patients with systemic lupus erythematosus in amounts varying from 0.004 to 0.4 μ/ml. The DNA was predominantly of low molecular weight, approximating 100 to 200 base pairs. Patients with lupus tended to have higher concentrations of plasma DNA than normal persons, with considerable overlap between the groups. Plasma DNA concentrations correlated inversely with titers of antibody to DNA but not strongly enough for prediction of either variable in individual cases. Many patients had high levels of plasma DNA and its antibody without clinical nephritis. These results indicate the ubiquity of plasma DNA and suggest the necessity of factors other than mere presence of DNA and its antibody for initiation of glomerular damage in systemic lupus erythematosus.     

Anaphylaxis-like reactions to corticosteroid therapy

This is a report of a 17-year-old male patient with a history of reactions characterized by the precipitous onset of urticaria, angioedema, and increased bronchospasm following therapy for status asthmaticus with either Solu-Medrol or Solu-Cortef. Intravenous challenge with 40 mg. of Solu-Medrol and methylprednisolone sodium succinate and oral challenge with 40 mg. of methylprednisolone produced dramatic confirmation of his history within 2, 11, and 35 minutes, respectively. Intravenous challenge with 4 mg. of Solu-Medrol and methylprednisolone sodium succinate, and oral challenges with 40 mg. of prednisolone and prednisone and 6 mg. of dexamethasone produced no adverse reactions. Direct skin testing revealed positive immediate reactions to pure methylprednisolone and hydrocortisone, whereas on passive transfer these reactions were negative for 4 and 48 hours. Negative skin test reactions were obtained to Solu-Medrol, methylprednisolone sodium succinate, methylprednisolone sodium acetate, prednisolone, prednisone, triamcinolone, dexamethasone, Solu-Cortef, hyrocortisone sodium succinate, and cortisone actate. Serial measurements during two intravenous challenges which produced anaphylaxis revealed no significant changes in 6 components of the complement system, fibrinogen, or the presence of split products.