5%冰醋酸辅助微波治疗尖锐湿疣173例疗效观察

尖锐湿疣 (CA)是由人乳头瘤病毒引起的疣状增生性疾病。随着性传播疾病 (STDs)患者的不断增加 ,CA发生率亦逐渐上升 ,在 STDs中居第三位 [1]电解、电灼、冷冻、激光及微波等治疗 CA已取得较好疗效 ,但其共同的缺点是治疗后部分患者容易复发。为了简单而有效地解决 CA治疗后的复发问题 ,从1 998年始 ,我们在微波治疗的同时辅用 5 %冰醋酸 ,取得了预防复发的良好效果。现报告如下。1　资料与方法1 .1　临床资料　 1 73例患者均为我院 CA门诊患者 ,其中男 1 0 2例 ,女 71例 ;年龄 1 5～ 6 3岁 ,平均 (3 1± 1 4)岁。皮疹分布于包皮、冠…     

Effect of electroacupuncture on expressions of VEGF and CD31 in MCAO model rats

Objective

To investigate the effect of electroacupuncture (EA) at Ganshu (BL 18) and Shenshu (BL 23) on vascular endothelial growth factor (VEGF) and platelet endothelial cell adhesion molecule-1 (PECAM-1)/CD31 around the cerebral infarction focus in middle cerebral artery occlusion (MCAO) rats and the possible mechanism, thus to provide a new strategy for the treatment of cerebral ischemic stroke by acupuncture.

Methods

A total of 180 healthy male Sprague-Dawley (SD) rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, 45 rats in each group. MCAO model was established using the modified line-embolus method in all rats except for those in the sham operation group; rats in the acupoint group were treated with EA at Ganshu (BL 18) and Shenshu (BL 23); rats in the non-acupoint group were treated with EA at the control points; rats in other 2 groups were only subjected to bundling without treatment. Ten rats in each group were randomly selected on the 3rd day, the 14th day and the 21st day after acupuncture stimulation to test the neurological function impairment. The expression levels of CD31 and VEGF were also detected.

Results

Compared with the model group and non-acupoint group, the neurological function score of the acupoint group was decreased at each time point, and the differences were statistically significant (P<0.05, P<0.01). The expressions of VEGF and CD31 in each group were the lowest on the 3rd day, reached the peak on the 14th day and still remained at high level on the 21st day. And the differences among groups were statistically significant both on the 14th day and the 21st day (P<0.05, P<0.01). Compared with the model group and the non-acupoint group, the expressions of VEGF and CD31 in the acupoint group were increased, and the differences were statistically significant (all P<0.05).

Conclusion

EA at Ganshu (BL 18) and Shenshu (BL 23) can significantly improve the neurological function score of MCAO model rats, and shows protective effect on cerebral ischemia. The protective mechanism may be related to the up-regulation of CD31 and VEGF expression around the cerebral infarction focus in the MCAO model rats and induction of angiogenesis.

     

Effect of electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) on the expression of EphB2 protein in cortex around cerebral infracted area of rat

Objective

To observe the effect of electroacupuncture (EA) on the expression of erythropoie-tin-producing hepatocyte receptor B2 (EphB2) in the cortex around the infracted area of middle cerebral artery occlusion (MCAO) rats at different timing, and to reveal the possible mechanism of acupuncture in the treatment of cerebral ischemia.

Methods

A total of 180 male Sprague-Dawley (SD) rats were randomly divided into a sham operation group, a model group, an acupoint group and a non-acupoint group, with 45 rats in each group. Rats in each group were further divided into three subgroups: postoperative 3 d, postoperative 14 d and postoperative 21 d groups, with 15 rats in each subgroup. The MCAO model was made by the modified occlusion method. The neurological function score, 2,3,5-triphenyl tetrazolium chloride (TTC) staining, immunohistochemistry assay, immunofluorescence double labeling method and Western blot were used to detect the corresponding indicators.

Results

The neurological impairment of rats was most obvious at postoperative 3 d, and then gradually improved with time, which was more significant in the acupoint group (P<0.05). The change of infarcted volume was consistent with the neurological function impairment. The number of EphB2 positive cells (EphB2⁺) around the infarcted area was decreased significantly at postoperative 3 d, and then gradually improved with time, which returned to the same level as that in the sham operation group at postoperative 21 d. The increase was most significant in the acupoint group (P<0.05), and the positive cell number was higher than that in the sham operation group (P<0.01). Western blot and immunohistochemistry results were basically consistent. Immunofluorescence displayed that EphB2⁺ and postsynaptic density-95 positive (PSD-95+) were co-expressed, after the MCAO operation, in the cortical neuron around the infracted area, and the number of co-expressing cells was increased gradually with time, which was most significant in the acupoint group (P<0.05).

Conclusion

Electroacupuncture at Ganshu (BL 18) and Shenshu (BL 23) can significantly improve the neurological function and cerebral infarcted volume ratio of MCAO rats, which may be related to the activation of EphB2 expression in cortex around the infracted area and the promotion of synaptic remodeling.

     

两定点连续缝合技术在后腹腔镜离断式肾盂成形术中的应用

目的比较后腹腔镜离断式肾盂成形术中"两定点连续缝合技术"和"间断缝合技术"的临床应用。方法回顾性分析2011年1月至2014年2月后腹腔镜离断式肾盂成形术107例。根据肾盂输尿管吻合方法分为"两定点连续缝合技术"组27例(A组),"间断缝合技术"组80例(B组)。对总手术时间、肾盂缩窄时间、肾盂输尿管吻合时间、术后住院时间、引流管拔除时间及漏尿等并发症进行比较。结果 A组手术无中转开放;B组2例中转开放。A组和B组总手术时间平均分别为167.7及191.4min(P=0.003);肾盂输尿管吻合时间平均为20.1及41.5min(P0.001);两组肾盂缩窄时间、术后引流管放置时间、术后住院天数比较差异无统计学意义。其中A组围手术期无漏尿病例,B组围手术期漏尿4例。术后3月复查B超提示A组肾积水消失16例,肾积水不同程度减轻11例;B组肾积水消失54例,肾积水不同程度减轻24例。结论后腹腔镜离断式肾盂成形术中采取"两定点连续缝合技术",可以降低输尿管吻合难度、缩短手术时间、减少术后漏尿发生率。     

前列腺增生患者前列腺液白细胞数量与血清PSA含量的关系

目的探讨前列腺增生患者前列腺按摩液（EPS）中白细胞含量与血清前列腺特异性抗原（PSA）升高之间的关系。方法 ELISA法测定78例前列腺增生患者的血清PSA含量,术前通过前列腺按摩获取前列腺液,并行前列腺液中的白细胞计数。分析EPS中白细胞含量与血清PSA升高的相关性,并比较不同血清PSA水平间（〈4ng/mL、4~10ng/mL、≥10ng/mL）的EPS中白细胞含量差异。结果前列腺增生患者血清PSA与EPS中白细胞密度和总数呈正相关关系,血清PSA升高组EPS中白细胞含量明显高于血清PSA正常组。结论前列腺增生患者血清PSA与EPS中白细胞的含量呈正相关,前列腺增生患者的PSA值增高可能与前列腺液中的白细胞增高有关。     

腹腔镜改良Duhamel术治疗慢传输型便秘

 目的评价腹腔镜改良Duhamel术治疗慢传输型便秘（STC）的疗效。方法回顾性分析8例行腹腔镜改良Duhamel术
的STC 患者的临床治疗及随访结果。结果本组患者手术均获成功,手术时间为160～220 min,手术出血量40～100 mL,术后肠
蠕动恢复时间为30～48 h,住院时间8～15 d,切口平均长度7 cm。所有患者术中无并发症发生。术后仅1 例患者发生肠梗阻,术
后1 个月时排便可控制在4 次以下,术后5 个月胃肠质量评分可接近正常人。结论腹腔镜改良Duhamel术治疗STC 安全有
效。     

3’-大豆苷元磺酸钠对大鼠脑缺血/再灌注损伤的保护作用

目的研究3’-大豆苷元磺酸钠对大鼠脑缺血/再灌注损伤的保护作用,并初步探讨其保护机制。方法大鼠大脑中动脉栓塞,制作脑缺血再灌注损伤模型,缺血后10 min,于舌下静脉给予不同剂量的3’-大豆苷元磺酸钠。再灌注24 h后,断头取脑,做TTC染色,测定脑梗死体积,另一组取血,离心后,取血清,测定血清一氧化氮合酶(NOS)、超氧化物歧化酶(SOD)的活性及一氧化氮(NO),丙二醛(MDA)的含量。结果与模型组相比,各剂量的3′-大豆苷元磺酸钠治疗组脑梗塞体积减小;3’-大豆苷元磺酸钠治疗组血清NOS和SOD的活性升高,NO含量升高,MDA含量降低。结论 3’-大豆苷元磺酸钠对大鼠脑缺血再灌注损伤具有一定的保护作用,其保护机制与3’-大豆苷元磺酸钠促进NO释放及增强氧自由基的清除有关。     

基于双荧光素酶报告基因系统的人源SREBP1基因启动子的活性研究

目的 构建固醇调节元件结合蛋白 1（SREBP1）启动子双荧光素酶报告基因表达载体,为研究针对 SREBP1靶点的天然活性抑制剂筛选奠定基础。方法 利用生物信息学软件,对SREBP1基因5′端上游5 000 bp序 列进行启动子预测与分析。应用 Gibson Assembly 方法构建启动子双荧光素酶载体,并将构建成功的 pGL3- SREBP1-pro重组质粒和内参质粒pRL-SV40共转染肝癌HepG2细胞并给予天然抑制剂大黄素,检测SREBP1转录活 性的抑制效果。结果 SREBP1基因5′端上游2 000 bp区域内有启动子特征序列,存在转录因子结合位点;重组质粒 经酶切及测序鉴定证实为阳性克隆,瞬时转染肝癌HepG2细胞后经双荧光素酶报告基因系统检测,所克隆的片段序 列具有启动子活性,该活性可被大黄素所抑制。结论 成功构建了pGL3-SREBP1-pro双荧光素酶报告基因表达载 体,并证实其活性,可为进一步用于针对SREBP1靶点的天然活性抑制剂筛选奠定     

三叶木通藤茎的化学成分和药理作用研究进展

对近年来有关三叶木通藤茎的化学成分及药理作用的研究进展进行综述。三叶木通是木通的基源植物之一,以干燥的藤茎入药。研究显示,其主要活性成分包括三萜及三萜皂苷类、酚醇及其苷类、咖啡酰奎尼酸类、有机酸类、苯丙素类、木脂素类、甾体类等,代表化合物有齐墩果酸、秦皮乙素、咖啡酸、β-谷甾醇;表现出抗肿瘤、抗氧化、抗炎、利尿、抑菌等药理作用。其抗肿瘤的作用机制包括诱导细胞凋亡、阻滞细胞周期、减少肿瘤血管生成、抑制肿瘤细胞侵袭和转移等,且对主要活性成分进行结构修饰,可有效提高抗肿瘤活性。     

白藜芦醇促进膀胱癌细胞对TRAIL凋亡诱导作用的研究

目的探讨白藜芦醇对膀胱癌细胞的作用及其与TRAIL联合对膀胱癌细胞的凋亡诱导作用及其可能的机制。方法MTT法检测白藜芦醇或/和TRAIL对膀胱癌细胞5637和T24体外生长能力的影响。流式细胞术测定白藜芦醇和/或TRAIL对细胞凋亡和线粒体跨膜电位的影响。Western blot检测白藜芦醇对死亡受体(DR)4、5与细胞凋亡相关蛋白表达水平的变化。结果 MTT证实白藜芦醇可抑制膀胱癌细胞体外生长能力,且呈剂量与时间依赖性。单独应用TRAIL未能抑制膀胱癌细胞的生长,但与白藜芦醇联合应用时可显著抑制细胞的增殖速度。白藜芦醇能够诱导膀胱癌细胞发生凋亡,而TRAIL则不能诱导细胞凋亡,但联合应用白藜芦醇和TRAIL可诱导膀胱癌细胞发生显著的凋亡。此外,在白藜芦醇或联合应用白藜芦醇与TRAIL可诱导肿瘤细胞丢失线粒体膜电位(ΔΨm),而单独应用TRAIL则未能诱导线粒体膜电位的丢失。Western blot结果表明,白藜芦醇可上调DR4和DR5的表达,并下调Bcl-2与survivin的表达。结论白藜芦醇可抑制膀胱癌5637和T24细胞增殖并诱导细胞凋亡。此外,白藜芦醇可以提高膀胱癌细胞对TRAIL诱导的细胞凋亡作用。单独应用白藜芦醇或与TRAIL联合应用可能成为临床膀胱癌治疗的新策略。