脂质体抗生素复合可生物降解型缓释系统治疗感染性骨缺损的研究进展

造成慢性感染性骨缺损的主要原因是创伤,在临床上一般治愈需要很长时间,且病症比较顽固,病程比较持久。最近几年对感染性骨缺损加大了研究的力度,尝试了各种治疗方法,本次研究采用脂质抗生素符合可生物降解型缓释系统进行治疗,探讨治疗效果。     

双源CT三期双能量扫描双期脑血管减影成像的初步应用

目的通过自身对比双能量减影与数字减影图像质量,探讨第二代双源CT三期双能量扫描模式双期脑血管减影成像的临床初步应用。方法 20例三期双能量双源CT脑血管检查按减影法分成A组:双能量减影;B组:100kv Neuro DSA数字减影。计算2组图像信噪比(SNR)与对比噪声比(CNR)及减影时间,按去骨程度及血管显示与切割程度,采取双盲法分别对图像评分(1-5分)。比较2组间统计学差异。结果减影时间A组短于B组(2.07±0.19min VS2.27±0.13min,P0.05)。A组动、静脉期SNR(29.89±12.20,30.49±7.34)均高于B组(24.42±9.99,27.17±6.59),差异有统计学意义(P0.05)。2组动、静脉期CNR差异均不显著(P0.05)。2组动、静脉期图像去骨评分差异均不显著(P0.05),A组动脉期血管评分低于B组(3.95±0.97 vs 4.95±0.23,P0.05),主要为部分颈内动脉管腔局部缺损。2组静脉期评分差异不显著。三期双能量扫描DLP平均为416.21±26.10 mGy·cm,较双期双能量扫描增加约10%。结论双源CT三期双能量扫描模式的双期脑血管成像,增加少许辐射剂量,可同时利用双能量与数字减影的优点来相互弥补诊断,推荐临床急诊脑血管检查。     

内镜下高频电凝联合根除幽门螺杆菌治疗隆起糜烂性胃炎的临床研究

目的 探讨应用内镜下高频电凝联合根除幽门螺杆菌(Hp)治疗隆起糜烂性胃炎(EGP)的价值和安全性.方法 将60例EGP患者分成A组(抑酸、保护胃黏膜、根除Hp药物治疗)和B组(抑酸、保护胃黏膜、根除Hp药物+高频电凝治疗同时进行)各30例,比较两种方法 的疗效.结果 A组单纯药物治疗后临床症状缓解(P＜0.05),但隆起病灶数消失不明显,仅有18.57%(47/253)的隆起病灶消失;B组治疗后,临床症状明显缓解(P＜0.05),97.15%(273/281),隆起病灶消失(P＜0.05),与单纯药物治疗组比较差异有统计学意义(P＜0.05).有6例出现轻微上腹胀或上腹隐痛,均在术后5 d内消失.结论 EGP单纯药物治疗疗效欠佳,内镜下高频电凝联合根除Hp治疗EGP不仅疗效确切,而且安全简便.     

HIF-1和BFGF、BFGFR与大肠癌的关系

缺氧诱导因子-1（Hypoxia inducible factor-1，HIF-1）是缺氧条件下广泛存在于哺乳动物和人体内的一种转录因子。大量的研究表明HIF-1在大肠癌组织中高表达，且与肿瘤的多种生物学行为有相关性，说明HIF-1在大肠癌的发病机制中扮演着一个相当重要的角色。细胞生长因子与肿瘤的关系已日益受到重视。多种生长因子在不同的肿瘤组织或体液中可被检测；多种细胞生长因子的受体在某些肿瘤组织中高表达；     

Bolus tracking技术在MSCT冠状动脉成像的应用

目的:研究应用Bolus tracking自动触发技术在16层螺旋CT冠状动脉成像时,选用不同阈值对血管质量的影响,评价其价值.方法:64例患者采用Bolus tracking技术,分别用100、130、160和190 HU的阈值,比较四组触发时间、实际触发值、升主动脉强化值和冠状动脉成像质量.结果:64例均完成Bolus tracking自动触发扫描,100 HU组130 HU组在触发时间和冠状动脉血管评价上无明显差异,但160 HU和190 HU组有明显差异,四组的实际触发值均有明显差异,而升主动脉强化值均无明显差异.结论:Bolus tracking技术可以为16层螺旋CT冠状动脉成像提供理想的扫描延迟时间,自动触发阈值选择在100～130 HU为佳.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

Expression of hypoxia inducible factor-1α and basic fibroblast factor in the tissues of colorectal adenocarcinoma

Objective To observe expression of hypoxia inducible factor-1α(HIF-1α)and basic fibroblast growth factor(BFGF)in the tissues of colorectal adenoearcinoma and analyze the relationship between expression of the two factors and biological behavior of colorectal adenocarcinoma.Methods The samples of colorectal adenocarcinoma(n=60)and para-adenocarcinoma(n=60)were taken from surgical resection patients and normal colorectal tissues (n=20) from patients with irritable bowel syndrome.The expression of HIF-1α and BFGF were detected by immunohistochemical staining (SP method).Results Positive rates of HIF-1αand BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%,and positive rates of HIF-1α and BFGF in para-adenocarcinoma tissues were 10.0%and 13.3%(P<0.05,respectively);HIF-1αand BFGF were not detected in normal intestinal mucosa.There was no significant correlation with HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the sexuality,age,tumor size,tumorposition and differentiation(P>0.05).A significant correlation between expression of the two factors and Dukes stage was observed (P<0.05).Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissuesof Dukes A and B stage were 47.2%and 52.7%,respectively.Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3%and 83.3%.respectively.There was a positive correlation between expression of the two factors and carcinogenesis of colorectal adenocarcinoma(r=0.4276.P<0.001).Conclusion The results showed that the enhanced expression of HIF-1 α and BFGF incolorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma,and HIF-1α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.     

蝮蛇解毒汤对蝮蛇咬伤致凝血功能障碍相关指标的影响

目的 观察蝮蛇咬伤诊治过程中相关凝血指标的改变情况,评价蝮蛇解毒汤在对蝮蛇咬伤致凝血功能障碍指标的影响。方法 收集有凝血功能障碍的蝮蛇咬伤患者114例,其中西医对照组患者51例,治疗组患者63例。对照组方案为西医常规治疗,治疗组在对照组治疗基础上加用蝮蛇解毒汤口服。统计分析两组出血凝血相关指标[常规凝血实验（CCT）、血小板计数（PLT）、血小板聚集功能（PAgT）、试验血栓弹力图（TEG）]。结果 治疗后两组凝血酶原时间（PT）、部分凝血酶原时间（APTT）、凝血酶时间（TT）、纤维蛋白原（FIB）、D-二聚体（D-dimer）均明显改善,且治疗组改善均优于对照组（P <0.05或P <0.01）;治疗组PLT较前改善（P <0.05）,但与对照组相比差异无统计学意义（P> 0.05）;两组PAgT中相关指标二磷酸腺苷（ADP）、胶原（COL）较前均改善显著（P <0.01）,治疗组优于对照组（P <0.05）,两组花生四烯酸AA差异无统计学意义（P> 0.05）;两组TEG中凝血因子活性R、纤维蛋白原功能K、纤维蛋白原功能α、血小板功能MA...     

桔梗不同配伍对乳腺癌高转移潜能细胞4T1增殖及侵袭的影响

目的:应用MTT比色法及Transwell试验检测桔梗配伍不同中药对乳腺癌高转移潜能细胞4T1增殖及侵袭能力的影响。方法:给予SD大鼠中药煎剂灌胃,制备含药血清。应用MTT比色法检测桔梗不同配伍含药血清对4T1细胞增殖的影响;应用Transwell实验检测桔梗不同配伍含药血清对4T1细胞侵袭能力的影响。结果:桔梗及桔梗配伍不同中药组含药血清干预24 h后,对4T1细胞的增殖均有一定的抑制作用。各组含药血清对乳腺癌高转移潜能细胞4T1的增殖抑制率分别为桔梗组30.75%、桔梗加麦冬组34.44%、桔梗加蛇床子组44.71%、桔梗加莪术组49.84%。Transwell实验结果显示,桔梗及桔梗配伍不同中药各组均能降低透过小室的4T1细胞数,对于乳腺癌高转移潜能细胞4T1的侵袭能力有显著的抑制作用,同模型组比较差异有统计学意义(P0.01)。同桔梗单用组比较,桔梗配伍不同中药组的抑制作用较强,其中桔梗配伍麦冬组及桔梗配伍蛇床子组效果显著,差异有统计学意义(P0.01),桔梗配伍莪术组在抑制作用方面虽然由于桔梗单用组,但差异无统计学意义(P0.05)。结论:桔梗及桔梗配伍不同治则中药对乳腺癌高转移潜能细胞4T1的增殖及侵袭有不同程度的抑制作用。