NF-κB结合元件缺失对NOX1启动子转录活性的影响

目的:构建含NADPH氧化酶1(NOX1)近端启动子区的pGL3萤光素酶报告基因载体及缺失NF-κB结合元件的相应载体,分别测定其相应活性,探讨NF-κB结合元件缺失对NOX1启动子区转录活性的影响。方法:采用PCR法克隆NOX1启动子区序列(1 415 bp),将目的片段与pGL3萤光素酶载体分别双酶切、纯化后进行连接(pGL3-NOX1-1415),测序鉴定;应用Alibaba 2.1软件分析NOX1近端启动子区,获取NF-κB结合元件;重叠PCR法将含该元件的启动子区域(88 bp)缺失,并构建相应载体(pGL3-NOX1-1327)。将两载体分别与pRL-TK内参质粒瞬时转染进入A549细胞,采用TNF-α(10μg/L)刺激细胞24 h,萤光酶标仪检测A549细胞的萤光素酶活性。结果:测序鉴定结果提示pGL3-NOX1-1415及NF-κB结合元件缺失的pGL3-NOX1-1327载体构建成功;细胞实验显示,TNF-α刺激后,转染pGL3-NOX1-1415的A549细胞萤光素酶活性明显强于对照组(P0.05),而转染NF-κB结合元件缺失的pGL3-NOX1-1327的细胞萤光素酶活性明显低于转染pGL3-NOX1-1415组(P0.05)。结论:TNF-α诱导A549细胞NOX1基因活化与NF-κB密切相关,NF-κB参与了TNF-α诱导的NOX1基因启动子的转录调控。     

脑梗死后微血管渗透性改变及出血性转化的预测

目的探讨缺血性脑梗死后急性期和亚急性期微血管渗透性改变及脑梗死后出血性转化(hemorrhagic transformation,HT)的预测。材料与方法收集缺血性脑梗死病人43例,急性期10例,亚急性期33例,进行常规MRI及DCE-MRI扫描。利用药代动力学模型计算容积转运常数Ktrans,比较每一个病人梗死区和对侧正常脑组织的Ktrans值以及出血组和非出血组的Ktrans值有无差异,比较不同时期梗死区强化对预测HT的意义。结果所有病人梗死区Ktrans值较对侧正常脑组织明显增高(P0.05)。10例在急性期有脑实质强化的病人都有继发出血,在亚急性期和慢性期15例病人有继发出血,18例没有继发出血,Fisher's精确检验有统计学意义(P0.05)。与亚急性期HT或没有HT的病人相比,急性期有HT组病人的Ktrans值明显增高(P0.05),但是亚急性期HT和非HT的病人Ktrans值比较没有统计学差异(P0.05)。结论急性期脑实质强化对HT的预测有更高的特异性,且渗透性比亚急性期更高。早期脑实质强化及HT与毛细血管内皮的紧密连接和基底膜损伤有关。后期脑实质强化及HT与侧枝循环的建立有关,DCE-MRI可以定量评估血脑屏障的渗透性,对进一步研究HT的分型提供更科学的依据。     

脂多糖刺激RAW264.7和Ana-1形态改变及细胞因子表达的差异

Objective To compare the morphologic change and cytokines expression in RAW264.7 and Ana-1 stimulated by lipopolysaccharide(LPS). Methods RAW264.7 and Ana-1 were cultivated with various concentrations of LPS(0.1 mg/L, 1 mg/L, 10 mg/L, 100 mg/L). MTT was performed to evaluate the proliferation ability of cells. Two kinds of cells were cultivated with 1 mg/L. Then,the concentrations of TNF-α,IL-1β,IL-6,IL-10 in culture medium were detected by ELISA in different times(0 h,4 h,8 h,12 h,24 h). Results The survival rates of RAW264.7 and Ana-1 (162.05±28.14)% and (159.92±20.43)% were significantly higher in 1 mg/L group than those of other groups. When stimulated with 1 mg/L LPS, both RAW264.7 and Ana-1 expressed cytokines in time-dependent manner (increased first and decreased finally). The concentrations of TNF-α and IL-10 in RAW264.7 group were higher than those Ana-1 group in 4 h only. IL-1β and IL-6,however,were in higher concentrations in RAW264.7 group than Ana-1 group all the time. Conclusions When stimulated with 1 mg/L LPS, both RAW264.7 and Ana-1 have higher survival rate. RAW264.7 and Ana-1 have different ability in expressing cytokines after being stimulated by LPS. Therefore,RAW264.7 and Ana-1 have variant response to LPS slightly.     

妇科微创手术在子宫肌瘤剔除术中的应用

目的:探讨阴式和腹腔镜下子宫肌瘤剔除术的微创价值。方法:选择2006年6月~2008年10月阴式子宫肌瘤剔除术患者45例(阴式组)及同期行腹腔镜子宫肌瘤剔除术的患者45例(腹腔镜组)和腹式子宫肌瘤剔除术50例(腹式组)患者的临床资料,比较3组的手术时间、术中出血量、术后体温恢复正常时间、术后排气时间和术后住院时间、术后病率。结果:阴式组手术时间最短,开腹组手术时间与腹腔镜组比较差异无统计学意义(P>0.05);术中出血量阴式组最少,腹腔镜组次之,且均少于开腹组(P<0.05);术后体温恢复正常时间阴式组与腹腔镜组比较差异无统计学意义(P>0.05),均短于开腹组;术后排气时间阴式组最短,腹腔镜组次之,均短于开腹组;术后住院时间阴式组与腹腔镜组比较差异无统计学意义(P>0.05),均短于开腹手术组(P<0.05)。术后病率比较中,差异无统计学意义(P>0.05)。结论:腹腔镜下和经阴道子宫肌瘤剔除术与经腹子宫肌瘤剔除术相比具有创伤小、术中出血少、术后恢复快及住院时间短等优点,阴式子宫肌瘤剔除术经过改变术前阴道消毒方法后术后病率、盆腔感染率显著下降,是值得推广和应用的微创术式。     

飞灰颗粒物氧化剂产生与金属形态分布关系研究

选取了5种燃煤飞灰颗粒物,其中4种经机械燃烧产生的飞灰(编号No．1～No．4),一种手烧茶炉产生的飞灰(编号No．5),以及一种燃木产生的飞灰颗粒物(编号No．6),利用脱氧核糖法(表征颗粒物经羟自由基催化氧化生物分子能力的常用体外实验方法),考察了飞灰颗粒物上金属形态浓度与颗粒物分别在无外加络合剂及加入各种络合剂即EDTA(乙二胺四乙酸)、NTA(胺三乙酸)和CIT(柠檬酸)条件下氧化剂产生能力的关系。实验结果表明,在无外加络合剂参与下,飞灰颗粒物上氧化物键合态及有机物键合态铁与其氧化剂产生能力相关性较好。络合剂的存在对飞灰氧化能力影响与金属形态浓度相关性的影响是不同的。     

环状弹性纤维溶解性巨细胞肉芽肿4例

环状弹性纤维溶解性巨细胞肉芽肿(annular elastolyticgiant cell granuloma,AEGCG)是临床少见的肉芽肿性皮肤病,临床上容易误诊和漏诊,2009年7月—2010年7月期间我院皮肤科门诊先后确诊4例患者,现报告如下。     

白石港水利血防工程对控制钉螺的效果研究

目的通过监测白石港水利血防工程所在区域的螺情变化,分析白石港水利血防工程的钉螺控制效果,为山丘型血吸虫病流行区选择较好的消灭钉螺方法提供科学依据。方法对白石港水系水利血防工程区域,从2005-2009年连续5年按系统抽样方法进行钉螺调查,分析工程前后活螺框出现率、活螺平均密度、钉螺面积变化。结果水利血防工程前的2005-2006年,活螺框出现率差异无统计学意义(χ2=0.12,P0.05);2007年,完工标段与未施工标段活螺框出现率比较差异有统计学意义(χ2=6.01,P0.05);2008年工程全部完工后,与2006年比较,活螺框出现率下降了90.38%,差异有统计学意义(χ2=301.56,P0.05),活螺平均密度下降了92.22%,钉螺面积下降了87.27%;2009年与2006年比较,活螺框出现率下降了90.83%,差异有统计学意义(χ2=385.29,P0.05),活螺平均密度下降了92.05%,钉螺面积下降了87.27%。结论水利血防工程能大幅度降低活螺框出现率、活螺平均密度和钉螺面积,控制钉螺效果明显。     

吉非替尼对博莱霉素致肺纤维化小鼠α平滑肌肌动蛋白的抑制作用

目的 观察吉非替尼对博莱霉素诱导肺纤维化小鼠a平滑肌肌动蛋白(a-SMA)表达的影响,探讨EGFR在肺纤维化上皮间质转分化中的作用.方法 将30只SPF级雌性BALB/c小鼠分为三组:对照组(气管滴人生理盐水)、纤维化组(气管滴人博莱霉素3 mg/kg )、纤维化吉非替尼干预组(气管滴人博莱霉素+吉非替尼灌胃20 mg/Kg ).实验第14天杀鼠取肺,肺组织石蜡切片行HE染色与Masson染色;RT-PCR法检测a-SMA的mRNA表达水平;免疫组化检测总EGFR,磷酸化EGFR及a-SMA表达.结果 纤维化吉非替尼干预组肺病理损伤较纤维化组减轻,气道上皮下胶原沉积减少,气道上皮及肺间质细胞磷酸化EGFR表达评分下降(P<0.05);同时,肺a-SMA的mRNA表达减少,免疫组化评分也明显降低(P<0.05).结论 吉非替尼抑制博莱霉素诱导小鼠肺纤维化可能与其抑制EGFR活性,下调a-SMA表达有关.     

新型氯化锂复合磷酸钙骨水泥的理化性能及成骨特性

BACKGROUND: Lithium chloride is a widely used inorganic ion inhibitor of glycogen synthase kinase-3β, and it can be combined with glycogen synthase kinase-3β to activate the classical Wnt/β-catenin pathway, thereby promoting human bone marrow mesenchymal stem cells and osteoblasts proliferation and accelerating bone repair. OBJECTIVE: To observe the physicochemical properties of novel lithium chloride/calcium phosphate cement, and to explore its osteoinductive biological property. METHODS: Calcium phosphate cement served as control group, and lithium chloride/calcium phosphate cement containing different lithium content as experimental groups. The setting time and compressive strength of bone cement in each group were detected, and the microstructure of the material surface observed under scanning electron microscopy. Bone cement and MC3T3-E1 cells were co-cultured in vitro, and the growth and adhesion morphology of MC3T3-E1 cells on the surface of bone cement were observed under the scanning electron microscope. Effect of bone cement extracts on cell proliferation was determined through MTT assay, and alkaline phosphatase kit used for determining alkaline phosphatase activity. RESULTS AND CONCLUSION: Lithium chloride/calcium phosphate cement had the same physicochemical properties to the calcium phosphate cement. Initial and final setting time, compressive strength and morphology of bone cement had no significant differences among groups. MC3T3-E1 cells grew and adhered well on the material surface. Results of MTT assay showed that compared with the calcium phosphate cement, the lithium chloride/calcium phosphate cement was better to improve osteoblast proliferation in vitro. In addition, the alkaline phosphatase activity in MC3T3-E1 cells was higher in experimental groups than the control group. These findings indicate that lithium chloride/calcium phosphate cement can maintain good physicochemical properties, and release lithium ions to promote bone formation.       

慢性正己烷中毒1例

某患者因长期接触低浓度正己烷而中毒,现将其临床表现、治疗与转归报告如下。