全文获取类型
收费全文 | 213074篇 |
免费 | 20154篇 |
国内免费 | 7797篇 |
专业分类
耳鼻咽喉 | 1271篇 |
儿科学 | 4752篇 |
妇产科学 | 3031篇 |
基础医学 | 41069篇 |
口腔科学 | 4062篇 |
临床医学 | 13523篇 |
内科学 | 35019篇 |
皮肤病学 | 3612篇 |
神经病学 | 16567篇 |
特种医学 | 3910篇 |
外国民族医学 | 66篇 |
外科学 | 14909篇 |
综合类 | 27569篇 |
现状与发展 | 31篇 |
预防医学 | 9407篇 |
眼科学 | 2491篇 |
药学 | 29609篇 |
30篇 | |
中国医学 | 8254篇 |
肿瘤学 | 21843篇 |
出版年
2024年 | 175篇 |
2023年 | 3151篇 |
2022年 | 4092篇 |
2021年 | 7895篇 |
2020年 | 7524篇 |
2019年 | 8277篇 |
2018年 | 7820篇 |
2017年 | 7996篇 |
2016年 | 7935篇 |
2015年 | 9196篇 |
2014年 | 13399篇 |
2013年 | 15775篇 |
2012年 | 13986篇 |
2011年 | 16307篇 |
2010年 | 13767篇 |
2009年 | 13112篇 |
2008年 | 12217篇 |
2007年 | 10778篇 |
2006年 | 9742篇 |
2005年 | 8230篇 |
2004年 | 7219篇 |
2003年 | 6107篇 |
2002年 | 4714篇 |
2001年 | 3969篇 |
2000年 | 3321篇 |
1999年 | 2845篇 |
1998年 | 2220篇 |
1997年 | 2074篇 |
1996年 | 1756篇 |
1995年 | 1780篇 |
1994年 | 1559篇 |
1993年 | 1297篇 |
1992年 | 1082篇 |
1991年 | 949篇 |
1990年 | 770篇 |
1989年 | 643篇 |
1988年 | 539篇 |
1987年 | 431篇 |
1986年 | 436篇 |
1985年 | 897篇 |
1984年 | 955篇 |
1983年 | 656篇 |
1982年 | 747篇 |
1981年 | 561篇 |
1980年 | 480篇 |
1979年 | 408篇 |
1978年 | 306篇 |
1977年 | 251篇 |
1976年 | 232篇 |
1975年 | 168篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
21.
《Advances in medical sciences》2022,67(1):163-169
PurposeThe variation in the immune response to Bartonella spp. infection in humans remains unclear. The present study compares the expression of selected interleukins, cytokines and cathelicidin (LL-37) in rheumatology clinic patients suffering from musculoskeletal symptoms with healthy blood donors. The patients had previously been tested for the presence of Bartonella henselae antibodies.MethodsGene expression of LL-37, interleukin (IL)-2, IL-4, IL-6, IL-12, interferon-(IFN)-γ, and tumor necrosis factor (TNF-α)-α was determined in blood samples using quantitative Polymerase Chain Reaction (qPCR). Statistical analysis was prepared with STATISTICA.ResultsStatistically significant differences in the mRNA levels of the tested cytokines (IFN-γ, TNF-α, IL-2, IL-4, IL-6, IL-12; p<0.0001) were observed between the healthy controls and patients; however, no difference was observed for LL37 mRNA (p ?= ?0.1974). No significant differences in mRNA expression were observed between IgG in anti-Bartonella seropositive and seronegative individuals (p>0.05). The only significant differences between the Bartonella spp. DNA positive and negative patients, indicated by PCR, were observed for TNF-α and IL-12 mRNA (p ?= ?0.0045 and p ?= ?0.0255, respectively).ConclusionA broadly similar immune response to the tested cytokines was observed among the participants irrespective of anti-Bartonella spp. IgG seropositivity. However, the Bartonella DNA-positive participants demonstrated significantly lower expression of IL-12 and TNF-α mRNA; this may indicate that these bacteria have a suppressive influence on the immune system. 相似文献
22.
《Advances in medical sciences》2022,67(2):262-268
PurposeEpidermal hyperplasia and the involvement of immune cells characterize the clinical picture of psoriasis. Among the several factors involved, attention has been focused on sirtuin 1 (SIRT1) - a deacetylase endowed with a variety of functions including the control of metabolic and inflammatory processes-, and on nicotinamide phosphoribosyltransferase (NAMPT), important for SIRT1 activation and involved in inflammatory events. The aim of the study was to analyze changes of SIRT1 and NAMPT expression in psoriatic skin.Patients and methodsSamples from healthy controls and psoriatic patients were subjected to immunohistochemical analysis.ResultsA strong downregulation of SIRT1 expression was observed in skin samples from psoriatic patients compared to healthy controls. This was accompanied by a parallel reduction of adenosine monophosphate-activated kinase (AMPK) expression and, more strikingly, by the disappearance of cells immunolabeled for its active, phosphorylated form (pAMPK). In both cases, analysis of the distribution of immunopositive cells revealed a shift towards reduced intensity of staining. In contrast, NAMPT expression was upregulated in psoriatic samples in line with its pro-inflammatory role. This was again more visible with an intensity-based distribution analysis that evidenced a shift towards more intensely immunostained cell populations.ConclusionsThe present data correlate in the same samples the expression of SIRT1, pAMPK/AMPK and NAMPT in psoriasis and open the way for novel pharmacological targets in the treatment of the disease. 相似文献
23.
24.
25.
《Clinical breast cancer》2022,22(6):507-514
Breast cancer (BC) is a highly metastatic, pathological cancer that significantly affects women worldwide. The mortality rate of BC is related to its heterogeneity, aggressive phenotype, and metastasis. Recent studies have highlighted that the tumor microenvironment (TME) is critical for the interplay between metastasis mediators in BC. BC stem cells, tumor-derived exosomes, circulatory tumor cells (CTCs), and signaling pathways dynamically remodel the TME and promote metastasis. This review examines the cellular and molecular mechanisms governing the epithelial to mesenchymal transition (EMT) that facilitate metastasis. This review also discusses the role of cancer stem cells (CSCs), tumor-derived exosomes, and CTs in promoting BC metastasis. Furthermore, the review emphasizes major signaling pathways that mediate metastasis in BC. Finally, the interplay among CSCs, exosomes, and CTCs in mediating metastasis have been highlighted. Therefore, understanding the molecular cues that mediate the association of CSCs, exosomes, and CTCs in TME helps to optimize systemic therapy to target metastatic BC. 相似文献
26.
《Mayo Clinic proceedings. Mayo Clinic》2022,97(7):1282-1293
ObjectiveTo assess the association between adherence to a Mediterranean lifestyle and changes in pain, and its characteristics over time in older adults.Patients and MethodsWe analyzed data from 864 and 862 community-dwelling individuals aged 65+ years from the Study on Cardiovascular Health, Nutrition and Frailty in Older Adults in Spain (Seniors-ENRICA) Seniors-ENRICA-1 (2008–2010 to 2012) and Seniors-ENRICA-2 (2015–2017 to 2019) cohorts, with a median follow-up of 2.8 and 2.4 years, respectively. Adherence to a Mediterranean lifestyle was assessed at baseline with the 27-item Mediterranean lifestyle (MEDLIFE) index. Pain changes over time were calculated with a pain scale that assessed the frequency, severity, and the number of pain locations both at baseline and follow-up. Multivariable-adjusted relative risk ratios (RRRs) were obtained using multinomial logistic regression.ResultsIn the pooled cohorts, after a median follow-up of 2.6 years, pain worsened for 697 participants, improved for 734, and did not change for 295. Compared with the lowest category of MEDLIFE adherence, those in the highest category showed an RRR of improvement vs worsening of overall pain of 1.85 (95% CI, 1.28 to 2.67; P-trend<.001). MEDLIFE adherence was also linked to improvement in pain frequency (RRR, 1.98; 95% CI, 1.31 to 3.01; P-trend=.001), pain severity (RRR, 2.00; 95% CI, 1.33 to 3.00; P-trend=.001), and a reduction in the number of pain locations (RRR, 1.68; 95% CI, 1.13 to 2.50; P-trend=.004). Limitations of this study are the use of self-reported lifestyle data.ConclusionA Mediterranean lifestyle was associated with improvement of pain characteristics in older adults. Experimental studies should assess the efficacy of an integral lifestyle approach for the management of pain in older adults. 相似文献
27.
28.
29.
30.