肝移植治疗晚期肝硬化

目的 肝移植治疗肝脏终末期病变。方法 采用改良的背驮式肝移植技术即保留肝后下腔静脉的全病肝切除,将供肝植于受肝原位,供、受体肝上下腔静脉,供、受体门静脉,供、受肝动脉行对端吻合;结扎供肝肝下下腔静脉。用ＦＫ５０６、晓翻和强的松三联免疫抑制剂抗排斥反应,加强术后监护和感染的控制。结果 例１、例２目前分别存活１１个月、８个月余,生活自理,例３因术后并发急性肾功能衰竭死亡,存活１４d。结论 肝移植是治愈肝脏终末期病谱的可靠方法。     

右肝管、左右肝管结石外科治疗(152例分析)

目的：探讨右肝管、左右肝管结石的外科治疗。方法：对我院10年来收治的152例右肝管、左右肝管结石病例的外科治疗经验进行总结。结果：152例中手术死亡4例（死亡率2.6%）,残石率13.8%,长期随访优良率91.4%。结论：依据结石分布的不同类型选择手术方式是合理、可行的。     

内镜辅助腹腔镜手术在早期结肠癌治疗中的应用

目的 探讨内镜辅助腹腔镜(双镜)手术在早期结肠癌治疗中的应用价值.方法 回顾性分析2002年3月至2007年12月间应用双镜手术治疗55例早期结肠癌患者的临床资料.结果 53例成功实施内镜定位、腹腔镜游离结肠肠段切除术,根据术中冰冻病理切片结果追加腹腔镜根治术11例;2例(3.6%)内镜定位后因小肠胀气中转开腹.平均手术时间90(55～240)min,术中平均出血量50(10～200)ml.51例(92.7%)术后2～3 d肛门排气,术后平均住院时间为5(2～15)d,无术后并发症.术后平均随访42(3～72)个月,除1例死于心肌梗死外,其余存活至今,均无肿瘤复发转移.结论 内镜辅助腹腔镜手术可充分发挥两者的优势,是早期结肠癌有效的治疗方法之一.     

幼儿腹膜后巨大神经母细胞瘤1例报告

幼儿腹膜后神经母细胞瘤较为少见,本院近期收治1例,现报告如下。患儿,男,3岁,因"无明显诱因反复腹胀10余天"入院。起病以来腹部膨隆,有增大倾向,无畏寒、发热,无恶心、呕吐、腹泻等症状。当地医院CT检查提示:左肾前方(相当于T12至S1水平)见一巨大软组织肿块,大小约12cm×9cm,共9个层面可     

N-乙酰半胱氨酸对大鼠小肠辐射损伤的防护作用

目的:探讨N-乙酰半胱氨酸(N-acetylcysteine,NAC)对小肠辐射损伤的防护作用及其作用机制.方法:60只雄性SD大鼠随机分为5组,单纯照射组(n=12)仅接受单次腹部照射;高、中、低剂量NAC给药组(n=12)均接受10 Gy X线单次腹部照射并分别给予腹腔注射NAC 300、200、50 mg/kg,连续7 d;正常对照组(n=12)给予腹腔注射1 ml 0.9%NaCl溶液,连续7 d.NAC在单次腹部照射前3 d开始经腹腔注射给药,至照射后第3日结束.照射后第3日,禁食12 h后处死大鼠,取血与末端回肠标本.光镜下观察并计算单位面积肠片上的肠腺存活率和绒毛数,测定血浆中D-乳酸含量和二胺氧化酶(diam-ine oxidase,DAO)活性,检测小肠组织中超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、还原型谷胱甘肽(glutathione,GSH)含量.结果:照射后大鼠末端回肠肠黏膜结构受到破坏,血浆中D-乳酸、DAO含量显著升高,小肠组织中SOD活性、GSH含量显著降低,MDA含量显著升高(P<0.01).不同剂量NAC给药处理后可不同程度地降低小肠黏膜绒毛数减少并提高肠腺存活率(P<0.05,P<0.01),抑制血浆中D-乳酸、DAO含量升高,提高小肠组织中SOD、GSH含量并减少脂质过氧化产物MDA的生成(P<0.05,P<0.01).结论:NAC通过增强机体抗氧化能力、减轻肠屏障功能障碍的严重程度.维护机体内氧化还原平衡以及小肠黏膜结构和功能的完整性.对小肠辐射损伤起到有效的防护作用.     

清热补益法防治辐射损伤相关肠屏障功能障碍研究现状

中医在防治辐射损伤相关肠屏障功能障碍方面具有独特疗效，临床多以清热补益法为基础，再结合患者不同兼证或变证而进行辨证论治、组方用药。就相关研究做一综述。     

全氟辛烷磺酸对大鼠仔鼠学习记忆能力的影响

Objective To study the effects of prenatal and postnatal perfluorooetane sulfonate (PFOS) exposure on spatial learning and memory, N-methyl-D-aspartate receptor 2B (NR2B) mRNA and protein level in frontal cortex and hippoeampus of rat pups and to explore the mechanism of developmental neurotoxicity induced by PFOS. Methods Twenty-eight pregnant mrs were randomly divided into three groups in proportion of 3: 2: 2,including control group (C), low dose group (L) and high dose group (H) by means of randomized number table, which respectively received 0,7.2, 14.4 mg/kg PFOS feed from pregnancy day 0 to postnatal day(PND)30 by free feedings. The animal models of prenatal and postnatal non-exposure (CC), prenatal exposure (LC and HC), postnatal exposure (CL and CH), and prenatal and postnatal exposure (LL and HH) to PFOS were established by cross-fostering method. The spatial learning and memory were measured by water maze experiment,the NR2B mRNA levels in frontal cortex of rat pups was determined with semi-quantitative RT-PCR, NR2B protein express in cerebral cortex (frontal and tempera]cortex) and hippocampus (CAI, CA3, CA4 and DG regions) of rat pups was detected by immunohistochemistry. Results The escape latency of CL, CH, LL and HH groups pups in water maze experiment were (99. 83±25.77) s, (111.30±17. 82) s, (106. 40±18. 71) s, (107.70±16. 85) s, and longer as compared with CC group[(54.90±26.69)s](q value were 4.349,4.773,6.026 and 5.641, respectively,P<0. 01). The number of errors of HH group rat pups entering dead end was (22. 30±7.56) at the training day 4,and it was significantly higher than that of CC group (9. 80±4. 64) (q=5. 173,P<0. 01). The NR2B mRNA levels of frontal cortex of pups in HC group at PND1 ,and LC group, HC group and HH group at PND14 were (0. 167±0. 008), (0. 364±0. 035), (0. 341±0. 030) and (0. 328±0. 045) respectively,which were significantly lower than CC group (0.271±0.060) and (0.465±0.067) (q values were 3. 547, 3. 739, 4. 597 and 5. 006, respectively, P<0.05) . The results of immunohistochemistry indicated that NR2B protein express of the hippocampus CA1 region of pups in LC group was (0.091±0.005),and showed significant lower than CC group which was (0. 123±0.009) at PND1 (q=5. 209 ,P<0. 05). At PND14, the effect of PFOS extended to cerebral cortex and hippocampus regions. At PND28, the effects of PFOS were showed in hippocampus CA1, CA3 and temporal cortex regions. Conclusion Prenatal and postnatal exposure to PFOS should result in the spatial learning and memory damage,and the mechanism might be possibly involved in the decrease of NR2B level in cerebra lcortex and hippocampal formation regions.     

不同方式建成3种反流性食管炎模型

运用贲门成形＋幽门结扎＋胃空肠Ｒｏｕｘ－ｅｎ－Ｙ吻合术,全胃切除＋食管十一指肠吻合术,及单屯食十二指肠吻合术,分别制作 单纯胃液食管反流（Ｇ）、单纯十二指肠内容物食管反流（Ｄ）及十二指肠胃内容物混合食管反流（ＤＧ）３种大鼠模型。于不同病程分批取得食管标本进行组织形态学研究。     

内镜辅助腹腔镜手术在早期结肠癌治疗中的应用

目的 探讨内镜辅助腹腔镜(双镜)手术在早期结肠癌治疗中的应用价值.方法 回顾性分析2002年3月至2007年12月间应用双镜手术治疗55例早期结肠癌患者的临床资料.结果 53例成功实施内镜定位、腹腔镜游离结肠肠段切除术,根据术中冰冻病理切片结果追加腹腔镜根治术11例;2例(3.6%)内镜定位后因小肠胀气中转开腹.平均手术时间90(55～240)min,术中平均出血量50(10～200)ml.51例(92.7%)术后2～3 d肛门排气,术后平均住院时间为5(2～15)d,无术后并发症.术后平均随访42(3～72)个月,除1例死于心肌梗死外,其余存活至今,均无肿瘤复发转移.结论 内镜辅助腹腔镜手术可充分发挥两者的优势,是早期结肠癌有效的治疗方法之一.     

超临界流体萃取和高压液相色谱法测定土壤中焰红染料

用有机溶剂做改性剂,对超临界流体二氧化碳（ＳＦ－ＣＯ２）进行修饰,同时使用无机盐和整合剂提高萃取物的回收率,萃取物用高效液相色谱进行定量分析,从而建立一种用ＳＦＣＯ２萃取土壤中极性化合物焰红染料（ｐｈｌｏｘｉｎｅ Ｂ）的检测方法。实验结果表明,甲醇对ＳＦＣＯ２有较强的修饰作用;丁胺和Ｎａ４ＥＤＴＡ具有促进ｐｈｌｏｘｉｎｅＤ的稳定性并提高土壤的解吸附水平。