肝细胞生长因子和丹参对大鼠急性肝损伤保护作用的实验研究

作者观察了肝细胞生长因子（HGF）、丹参对DDl4所致大鼠急性肝损伤的影响，结果发现，HGF组、丹参组以及西药联用组对急性肝损伤大鼠的血清ALT、α-肿瘤坏死因子（TNF-α）、丙二醛（MDA）以及肝组织匀浆MDA、肝组织钙含量的升高等均有不同程度的降低作用，且能使CCl4所致降低的血清及肝组织匀浆SOD活力明显升高。光镜及电镜下观察，用药组肝细胞病变软CCl4组有明显改善。本文结果表明，HGF、丹参对CCl4所致大鼠急性肝损伤有良好的保护作用，且HGF、丹参联用效果优于单用．     

不同剂量紫外线照射对血液抗氧化作用的影响

实验用不同剂量短波紫外线（ＵＶ－Ｃ）照射家兔离体血液，采用化学发光法（ＣＬ）和分光光度法分别测定血浆超氧化物歧化酶（ＳＯＤ）活力和丙二醛（ＭＤＡ）含量。未照射血液血浆ＳＤＯ活力、ＭＤＡ含量分别为９４６．６７±５７．６５Ｕ／ｍｌ、１７．７６±３．３９ｎｍｏｌ／ｍｌ。小剂量（１５秒）照射后ＳＯＤ活力显著高于未照射血液（Ｐ＜０．０１），剂量增加后（３０秒）ＳＯＤ活力下降并接近未照射水平，照射１分钟后活力明显下降（Ｐ＜０．０５），并随短波ＵＶ－Ｃ剂量（３、６分钟）增大下降增多（Ｐ＜０．００１）。较小剂量ＵＶ－Ｃ（１５秒、３０秒、１分钟）照射后ＭＤＡ含量无明显变化，ＵＶ－Ｃ照射３分钟时ＭＤＡ含量开始出现明显升高（Ｐ＜０．０５），并随剂量加大（６分钟）进一步增高（Ｐ＜０．００１）。结果提示小剂量ＵＶ－Ｃ照射能够增强机体抗氧化能力，大剂量照射则使机体抗氧化能力下降，氧化损伤作用增强；并且说明ＵＶ－Ｃ照射所致机体脂质过氧化损伤与抗氧化能力下降，自由基生成增多、消除不足有关     

别嘌呤醇对伴高尿酸血症慢性心力衰竭急性发作患者血清NO、MDA、SOD及肱动脉内皮依赖性舒张功能的影响

目的通过观察试验组和对照组用药前后慢性心力衰竭急性发作患者血浆一氧化氮（NO）丙二醛（MDA）超氧化物歧化酶（SOD）及肱动脉内皮依赖性舒张功能（EDD）的变化来探讨别嘌呤醇对伴高尿酸血症慢性心力衰竭急性发作患者内皮功能及氧自由基的影响。方法78例慢性心力衰竭急性发作患者被随机分为别嘌呤醇组（常规治疗＋别嘌呤醇，38例）和对照组（常规治疗，40例），分析NO、MDA、SOD在1周治疗前后浓度的变化及EDD的变化。结果别嘌呤醇治疗7d后，与对照组相比，试验组血SOD浓度明显升高（P〈0．05），血MDA显著下降（P〈0．05），而试验组NO及肱动脉EDD有上升趋势；但差异无统计学意义。结论别嘌呤醇能显著增加慢性心力衰竭急性发作患者血SOD，降低血MDA，因而减轻此类患者氧化应激的程度，进而可能对该类患者内皮舒张功能产生积极影响。     

二甲氨基乙醇和复方氨基酸溶液对衰老 大鼠皮肤胶原代谢和自由基的影响

目的 探讨真皮内注射二甲氨基乙醇（DMAE）和复方氨基酸溶液（AA）对皮肤胶原代谢和自由基的影响.方法 80只大鼠随机分成衰老治疗组（60只）、衰老对照组（10只）和正常对照组（10只）.皮下注射半乳糖制备衰老模型.衰老治疗组从第18天开始,在两侧臀部真皮内分别注射0.2％ DMAE＋AA、0.1％ DMAE＋AA、0.2％ DMAE、0.1％ DMAE、AA、生理盐水（NS）,每周1次,连续4周.观察各组皮肤苏木精-伊红染色法（HE）切片组织学变化并测定皮肤中含水量、超氧化物歧化酶（SOD）活力、羟脯氨酸（HYP）和丙二醛（MDA）含量.结果 0.2％DMAE＋ AA组、0.1％ DMAE＋AA组表皮、真皮厚度和胶原纤维面积均高于衰老对照组,差异有统计学意义（P＜0.05）.0.2％ DMAE＋AA组、0.1％ DMAE＋ AA组与其他各衰老组相比,HYP含量增加、SOD活力升高、MDA含量降低,其组间差异具有统计学意义（P＜0.05）.正常对照组、0.2％ DMAE＋AA组、0.1％DMAE＋ AA组之间HYP含量差异无统计学意义（P＞0.05）.各组大鼠皮肤含水量差异无统计学意义（P＜0.05）.结论 真皮内注射0.2％ DMAE＋AA、0.1％ DMAE＋AA能显著增加表皮、真皮厚度和胶原纤维面积及HYP含量,提高SOD活力、降低MDA含量,表明其有清除皮肤自由基、提高抗氧化能力和促进皮肤胶原代谢的作用,从而延缓皮肤衰老.     

Vascular endothelial growth factor and oxidative damage in cancer

OBJECTIVES: VEGF may be an indicator for the angiogenic potential of a tumor and stimulates NO which plays complex roles in cancer. In our study, we investigated the levels of MDA, NO and VEGF in the plasma of various types cancer patients (untreated, yet). DESIGN AND METHODS: The level of VEGF was determined by using ELISA. Plasma MDA, NO and VEGF levels were measured in 45 patients with various cancer types and in 21 healthy subjects. RESULTS: Plasma MDA and VEGF levels were significantly higher than those of the healthy subjects (p < 0,0001). NO levels of the patients were significantly lower vs. the healthy subjects (p < 0,001). CONCLUSIONS: Increased plasma VEGF and MDA concentrations and decreased plasma NO levels have been found in patients with various types of human cancer. Howewer, the prognostic and clinical significance of plasma VEGF in cancer patients is unknown.     

The influence of the hemodialysis treatment time under oxidative stress biomarkers in chronic renal failure patients

SUMMARY: Oxidative stress possibly helps to promote the progression and complication of chronic renal failure (CRF). Hemodialysis (HD) may aggravate oxidative stress. In addition long time of treatment may intensify the oxidative stress. Thus, the aim of this study was to evaluate the effect of prolonged HD treatment under parameters of the oxidative stress. METHODS: Plasmatic thiobarbituric acid reactive substances (TBARS), plasmatic malondialdehyde (MDA), blood delta-aminolevulinate dehydratase (ALA-D) activity, ALA-D reactivation index, and erythrocytic reduced glutathione (GSH) were measured into two different groups of HD patients: recent treatment (n=36; HD duration: 17.7+/-1.71 months), and long time of treatment (n=26; HD duration: 82.2+/-6.32 months), and in a control group (n=40). RESULTS: Plasmatic TBARS and MDA levels were both elevated in HD patients. However, only MDA levels had positive correlation with time of HD treatment. Blood ALA-D activity was decreased in HD patients. The ALA-D reactivation index showed increase in HD patients, and it had correlation with the time of HD treatment. Erythrocytic GSH levels were increased in HD patients. CONCLUSIONS: Our results indicated that MDA levels and ALA-D reactivation index may be the better biomarkers to evaluate chronic oxidative stress in comparison with others markers analyzed in this study.     

基于Nrf2/Keap1信号通路探讨金盏银盘水提液对四氯化碳诱导急性肝损伤小鼠抗氧化应激作用

目的 探究金盏银盘Bidens biternata（Lour.）Merr.&Sherff.水提液对四氯化碳诱导的急性肝损伤改善作用及其机制。方法 将60只雄性昆明小鼠随机分为对照组、模型组、水飞蓟素（0.15 g/kg）组和金盏银盘低、高剂量（3.9、7.8 g/kg）组,每组12只。各组小鼠连续ig给予相应药物7 d,末次给药后除对照组外均ip 0.4%四氯化碳构建急性肝损伤小鼠模型。24 h后收集小鼠血清及肝脏组织。用生化试剂盒检测血清中天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、超氧化物歧化酶（SOD）活性及丙二醛（MDA）含量,并取肝组织匀浆检测谷胱甘肽过氧化物酶（GSH-Px）活性。采用苏木精-伊红染色（HE）切片观察小鼠肝脏病理变化。Western blotting测定肝组织中核因子E₂相关因子2（Nrf2）、抗体Kelch样ECH相关蛋白1（Keap1）、血红素氧合酶1（HO-1）蛋白的表达情况。结果 与模型组比较,金盏银盘水提液组小鼠组织病理学观察发现肝脏炎症细胞浸润明显减少,血清AST、ALT、MDA水平降低,血清SOD和肝组织GSH-Px活性升高（P＜0.05、0.01）;金盏银盘水提液组可以增加小鼠肝脏Nrf2和HO-1蛋白表达,抑制Keap1蛋白表达。结论 金盏银盘能够有效改善四氯化碳诱导的急性肝损伤,作用机制与激活Nrf2/Keap1信号通路抑制氧化应激损伤有关。     

联合应用参麦和丹参注射液对急性心肌梗死缺血/再灌注心肌的保护作用

目的　探讨联合应用参麦和丹参注射液对急性心肌梗死 (AMI)早期缺血心肌和缺血 /再灌注心肌的保护作用。方法　采用化学分光比色法检测分析了 2 2例联合应用参麦、丹参注射液的AMI患者血清超氧化物歧化酶 (SOD)及丙二醛 (MDA)的动态变化 ,并与 18例常规治疗组及 2 0例健康对照组的检测结果进行了比较。结果　所有急性心肌梗死患者入院即刻检测血清中SOD水平均较健康对照组降低〔(81 6 2± 8 81)nU/mLvs (10 4 0 2± 10 84 )nU/mL ,P <0 0 1〕 ,而MDA水平升高〔(8 14± 2 76 )nmol/mLvs (4 0 6± 1 2 7)nmol/mL ,P <0 0 0 1〕 ;随治疗时间推移 ,加用参麦与丹参注射液组与常规治疗组比较SOD及MDA水平有明显差异 (P <0 0 1) ,SOD于治疗后第 1天降至最低水平〔(73 11± 7 0 2 )nU/mLvs (6 4 32± 4 99)nU/mL〕 ,随后逐渐升高 ;MDA于治疗后第 1天升至最高水平〔(9 94± 2 2 )nmol/mLvs (11 84± 2 95 )nmol/mL〕 ,随后下降。结论　参麦和丹参注射液合用具有减轻氧自由基 (OFR)对心肌的损伤作用 ,对缺血心肌和缺血 /再灌注心肌具有一定的保护作用。     

Protective effects of CVPM on vascular endothelium in rats fed cholesterol diet

BACKGROUND: The cardiovascular protective mixture (CVPM) is a concoction of nine Chinese traditional medicines: Dan-shen root, Szechwan lovge rhizome, Chinese angelica, Hawthorn fruit, Safflower, Peach seed, Red peony root, earthworm, and membranous milkvetch root. These medicines are used to cure cardiovascular disease in China. METHODS: Animal models were established by feeding the Sprague-Dawley (SD) rats with lipid-rich forage. Serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were measured. Malondialdehyde (MDA) content was determined to monitor lipid peroxidation. The 6-keto-prostaglandin F(1alpha)(6-keto-PGF(1alpha)) concentration was measured by radioimmunoassay to investigate the content of prostacyclin (PGI(2)). Electron microscope (JEM-1200EX) was used to observe the microstructure of the vascular endothelium. Rat aortic endothelial cell was cultured to investigate the effect of CVPM on vascular endothelial cell in vitro. RESULTS: CVPM inhibited the accumulation of TC, LDL-C, and MDA in vivo, when the rats were fed with cholesterol diet. CVPM promoted synthesizing and excreting of PGI(2), since it is capable of activating the proliferation of vascular endothelium in vitro. Electron micrographs showed that CVPM had notable protective effect on the vascular endothelium and prevented the shedding of these cells from subendothelial layer. CONCLUSIONS: CVPM could ameliorate the internal environment in which vascular endothelial cells lived, and activate the proliferation of these cells. Through these mechanisms, CVPM protect vascular endothelial cell from being harmed by excess cholesterol in vivo.     

白花败酱草乙醇提取物对小鼠抗氧化作用的影响

目的：研究白花败酱草乙醇提取物对小鼠抗氧化作用的影响。方法取昆明种小鼠32只,随机分为生理盐水组,5.0、10.0、20.0 mg/kg白花败酱草乙醇提取物组。连续给药10 d,次日小鼠拉颈椎脱臼处死,取心、脑、肝等组织,检测心、脑、肝等组织中的超氧化物歧化酶(SOD)的活性、丙二醛(MDA)的含量。结果10.0、20.0 mg/kg白花败酱草乙醇提取物能显著升高小鼠心、脑、肝等组织中SOD活性(P<0.05或P<0.01),显著降低心、脑、肝等组织中MDA含量(P<0.05或P<0.01)。结论白花败酱草乙醇提取物可清除体内脂质过氧化物,减轻机体的过氧化损伤,具有明显的抗氧化作用。