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目的观察壳聚糖纳米粒子载体载基因在胶质瘤细胞中表达及其对神经细胞的保护作用。方法采用免疫细胞化学检测胶质细胞源性神经营养因子(GDNF)在胶质细胞瘤细胞中的表达,采用细胞增殖实验(MTT法)观察GDNF对神经细胞的营养作用。结果GDNF在胶质细胞瘤细胞中有表达,在应用纳米粒子载体载基因进入细胞内后GDNF得到高效表达,并保护神经细胞,促进细胞增殖。结论应用纳米粒子载体载基因进入细胞内后可有效提高GDNF表达.并保护神绎细胞.促进细胞增殖。对脊髓损伤后应用这一方法治疗提供理论依据。 相似文献
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Julie A. Christianson Janelle M. Ryals Kenneth E. McCarson Douglas E. Wright 《The journal of pain》2003,4(9):493-504
Studies were carried out in streptozotocin-treated diabetic mice to evaluate their behavioral responses to different noxious stimuli. In opposition to rats, streptozotocin-injected diabetic mice display a persistent hypoalgesia to non-noxious mechanical stimulation (von Frey monofilament). Similarly, nocifensive responses of diabetic mice to formalin injection were significantly reduced in both acute and inflammatory phases. However, no overt differences were detected between nondiabetic and diabetic mice in their sensitivity to noxious heat (radiant heat), cold (acetone), or noxious mechanical (pinprick) stimuli applied to the hind paw. To evaluate whether neurotrophin treatment could normalize the sensory deficits, nerve growth factor (NGF) or glial cell line-derived neurotrophic factor (GDNF) was administered intrathecally to diabetic mice for 3 weeks. Neurotrophin-treated mice were also compared to mice that received insulin for 3 weeks. Both NGF and insulin treatment significantly restored mechanical and chemogenic behavioral responses of diabetic mice. In contrast, GDNF treatment only reversed behavioral responses to chemogenic stimuli during the acute phase of the formalin test. These results demonstrate that diabetic mice develop reduced sensitivity to mechanical and chemical stimuli. Furthermore, these studies show that dorsal root ganglion neurons in diabetic mice are responsive to treatment with either NGF or GDNF; however, these 2 neurotrophins differ in their ability to affect distinct somatosensations. 相似文献
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Few studies have compared the processing of endogenous human amyloid precursor protein (APP) in younger and older neurons. Here, we characterized LUHMES cells as a human model to study Alzheimer's disease-related processes during neuronal maturation and aging. Differentiated LUHMES expressed and spontaneously processed APP via the secretase pathways, and they secreted amyloid β (Aβ) peptide. This was inhibited by cholesterol depletion or secretase inhibition, but not by block of tau phosphorylation. In vitro aged cells increased Aβ secretion without upregulation of APP or secretases. We identified the medium constituent glial cell line-derived neurotrophic factor (GDNF) as responsible for this effect. GDNF-triggered Aβ release was associated with rapid upregulation of the GDNF coreceptor “rearranged during transfection” (RET). Other direct (neurturin) or indirect (nerve growth factor) RET activators also increased Aβ, whereas different neurotrophins were ineffective. Downstream of RET, we found activation of protein kinase B (AKT) to be involved. Accordingly, inhibitors of the AKT regulator phosphatidylinositol-3-kinase completely blocked GDNF-triggered AKT phosphorylation and Aβ increase. This suggests that RET signaling affects Aβ release from aging neurons. 相似文献
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神经营养因子能够调节神经元的生长,并维护神经元可塑性,神经营养因子的缺乏能够导致神经细胞的易损性增加甚至凋亡,本文就目前关于神经营养因子‐3和胶质细胞源性神经营养因子在抑郁症发病过程中的作用进行了讨论,以期为抑郁症的基础研究和临床诊断及治疗提供思路。 相似文献
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To date, most studies of Shc family of signaling adaptor proteins have been focused on the near-ubiquitously expressed ShcA, indicating its relevance to age-related diseases and longevity. Although the role of the neuronal ShcC protein is much less investigated, accumulated evidence suggests its importance for neuroprotection against such aging-associated conditions as brain ischemia and oxidative stress. Here, we summarize more than decade of studies on the ShcC expression and function in normal brain, age-related brain pathologies and immune disorders with a focus on the interactions of ShcC with signaling proteins/pathways, and the possible implications of these interactions for changes associated with aging. 相似文献
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Xiuliang Wang Chendong YuanLi Xiang Xiaoqing LiZhanbo Zhao Xianqing Jin 《Journal of pediatric surgery》2013