siRNA干扰血小板内皮细胞黏附因子-1表达对人鼻咽癌耐药细胞CNE1/R多药耐药性的影响

目的 探讨抑制血小板内皮细胞黏附因子-1(PECAM-1)表达对多药耐药基因1(MDR-1)及其编码产物P-糖蛋白(P-gp)的影响.方法 设计并化学合成针对PECAM-1的三条siRNA序列,命名为si-PECAM1,2,3,脂质体转染法将其转入CNE1/R,采用Semi-qRT-PCR、Western blot方法检测并筛选出抑制效果最好的si-PECAM序列,将筛选出的si-PECAM转入CNE1/R,采用Semi-qRT-PCR、Western blot方法检测抑制PECAM-1表达前后MDR-1及其编码产物P-gp的变化情况.结果 Semi-qRT-PCR的结果显示,设计三条si-PECAM序列均有效,以si-PECAM3干扰效果最明显(P<0.001),Western blot结果显示与Semi-qRT-PCR的结果一致;转染si-PECAM3 48 h后做Semi-qRT-PCR的结果显示抑制PECAM-1表达后MDR-1表达明显下调(P<0.001),Western blot结果与Semi-qRT-PCR的结果一致,抑制PECAM-1表达后MDR-1编码产物P-gp的表达也明显下调(P<0.001).结论 抑制CNE1/R中PECAM-1表达后MDR-1及其编码产物P-gp的表达均明显下调,提示PECAM-1可能参与X射线辐射诱导人鼻咽癌细胞发生多药耐药.     

MiR-486-3p targeting ECM1 represses cell proliferation and metastasis in cervical cancer

MicroRNAs (miRNAs) regulate gene expression and are involved in cervical cancer. But the molecular mechanism is still unclear. Here, miRNA profile of cervical cancer was performed and demonstrated that miR-486-3p decreased in specimens of cervical cancer patients. In addition, our clinical data show that decreased miR-486-3p was associated with metastasis in cervical cancer patients. ECM1 was predicted and velified as a target gene of miR-486-3p. Overexpression of miR-486-3p inhibited cell growth and metastasis by targeting ECM1. In a conclusion, these findings suggest that miR-486-3p is a tumor suppressor miRNA and induction of miR-486-3p is a potential strategy to inhibit cervical cancer progression.     

5-AZA抑制肝癌细胞BEL7402代谢方式转换

目的研究去甲基化药物5-AZA对miR-34a和miR-34c在肝癌细胞中表达的影响及其抑制肝癌细胞糖酵解的分子机制。方法用real-time PCR法检测肝癌细胞系中miR-34a/c的表达以及糖酵解途径关键酶的表达;在肝癌细胞中过表达miR-34a、miR-34c或用5-AZA处理肝癌细胞,用乳酸检测试剂盒、葡萄糖检测试剂盒分析miR-34a/c及5-AZA对肝癌细胞BEL7402糖酵解的影响;设计拯救实验研究5-AZA、miR-34a/c与肝癌细胞糖酵解调控的关系。结果 5-AZA可以诱导BEL7402肝癌细胞内源性miR-34a、miR-34c的表达上调(P0.01);过表达miR-34a/c可以抑制糖酵解关键酶LDH-A的表达(P0.05);LDH-A是miR-34a/c的潜在靶基因;敲低miR-34a/c的表达可以降低5-AZA对肝癌细胞BEL7302糖酵解途径的抑制作用。结论 5-AZA通过诱导miR-34a/c表达上调,发挥抑制肝癌细胞代谢方式转换的功能。     

MicroRNA-424在肿瘤发生中的作用和机制研究进展

MiR-424是miR-16家族成员。近年研究表明miR-424与肿瘤的发生、发展及治疗预后密切相关。本文对miR-424在乳腺癌、宫颈癌、肺癌、肝癌及结直肠癌等多种肿瘤及白血病中的表达变化、作用及机制进行综述。研究发现miR-424的表达受多种因素的影响，miR-424可作为肿瘤诊断、分期、预后的生物标记物，可用于明确肿瘤范围，也可作为肿瘤的治疗靶物。     

Japanese orthopaedic association cervical myelopathy evaluation questionnaire (JOACMEQ): Part 5. Determination of responsiveness

Background

In 1999, the Japanese Orthopaedic Association decided to develop a new Cervical Myelopathy Evaluation Questionnaire (JOACMEQ). The final version of the JOACMEQ, comprising 24 questions and five domains (cervical spine function (CF); upper extremity function (UF); lower extremity function (LF); bladder function (BF); and quality of life (QOL)), was established after three nationwide investigations. The fourth investigation, reported in this paper, was performed to confirm the responsiveness of the questionnaire.

Effects of miR-200a and FH535 combined with taxol on proliferation and invasion of gastric cancer

Gastric cancer is one of the most common cancers in the world; taxol displayed modest efficacy as first-line chemotherapy for gastric cancer, conversely, it has limitations used alone. β-catenin is a multifunctional oncogenic protein and the elevation in expression and activity of β-catenin has been implicated in many cancers. Therefore, we assume that the inhibition of β-catenin can enhanced the efficacy of taxol. The purpose of this study was to investigate the inhibitory effect of miR-200a mimics, FH535 combined with taxol on proliferation and invasion of human gastric cancer cell lines SGC-7901 and BGC-823. In the current study, we identified that the combination of FH535 and miR-200a with taxol had potent growth-inhibitory and pro-apoptotic effects. Further, similar results were also observed in vivo, intratumoral injection of FH535, taxol and miR-200a mimics which also delayed tumor growth in nude mice harboring subcutaneous SGC-7901 xenografts. Collectively, miR-200a and FH535 can enhance the inhibitory effect of taxol on cell proliferation and moderate the invasion of human gastric cancer.     

Whole‐body radiofrequency coil for 31P MRSI at 7 T

Widespread use of ultrahigh‐field ³¹P MRSI in clinical studies is hindered by the limited field of view and non‐uniform radiofrequency (RF) field obtained from surface transceivers. The non‐uniform RF field necessitates the use of high specific absorption rate (SAR)‐demanding adiabatic RF pulses, limiting the signal‐to‐noise ratio (SNR) per unit of time. Here, we demonstrate the feasibility of using a body‐sized volume RF coil at 7 T, which enables uniform excitation and ultrafast power calibration by pick‐up probes. The performance of the body coil is examined by bench tests, and phantom and in vivo measurements in a 7‐T MRI scanner. The accuracy of power calibration with pick‐up probes is analyzed at a clinical 3‐T MR system with a close to identical ¹H body coil integrated at the MR system. Finally, we demonstrate high‐quality three‐dimensional ³¹P MRSI of the human body at 7 T within 5 min of data acquisition that includes RF power calibration. Copyright © 2016 John Wiley & Sons, Ltd.