赤子爱胜蚓五种纤溶酶组分的分离纯化及对纤维蛋白原酶解的初步研究

经硫酸铵盐析沉淀和ＰＡＧＥ制备电泳,从赤子爱胜蚓粗品中分离纯化出五种纤溶酶组份,在ＰＡＧＥ中均呈现单一带。组份Ⅶ、Ⅷ、Ⅹ能将纤维蛋白原中α、β、γ链依次降解,对α链亲和力最大。     

地龙注射液对屋尘螨致敏气道上皮细胞TGF-β1/Smad2表达的影响

 目的 观察地龙注射液对细胞哮喘模型中转化生长因子（TGF）-β₁、Smad2表达的影响,探讨地龙在支气管哮喘治疗中的作用机制及价值。方法 给予人支气管上皮细胞系BEP2D（HBE）以不同的干预和治疗,分为：单纯培养组即对照组（A组）,屋尘螨(dermatophagoides pteronyssinus,Derp1)（10 μg·mL^-1）组（B组）,Derp1（10 μg·mL^-1）+ 地塞米松（dexamethasone,DEX）（1×10^-6mol·L^-1）组（C组）,Derp1（10 μg·mL^-1）+地龙注射液（0.9 mg·mL^-1）组（D组）,Derp1（10 μg·mL^-1）+地龙注射液（9 mg·mL^-1）组（E组）, Derp1（10 μg·mL^-1）+地龙注射液（18 mg·mL^-1）组（F组）,培养72 h,RT-PCR法检测各组细胞TGF-β₁ mRNA, Smad2 mRNA 表达;并收集以上各组上清液,作用于人胚肺成纤维细胞（HLF）72 h,免疫细胞化学法检测纤维连接蛋白(Fibronectin,FN)表达。结果 B组TGF-β₁mRNA, Smad2 mRNA表达较A组明显升高（P<0.01）;各地龙组及C组,TGF-β₁ mRNA、Smad2 mRNA表达低于B组,有显著性差异(P<0.01),与A组比较均无显著性差异（P>0.05）;各组HLF中FN表达与HBE各对应组TGF-β₁ mRNA表达呈正相关性。结论 地龙注射液有抑制哮喘气道重构的作用,其机制可能与抑制TGF-β₁/Smad2 信号通路有关。     

蛭龙活血方对颈动脉粥样硬化并血瘀证患者临床疗效观察

目的:观察蛭龙活血方对颈动脉粥样硬化并血瘀证患者的临床疗效。方法:65例入选病人随机分为两组,对照组予口服辛伐他汀治疗,治疗组予蛭龙活血方加辛伐他汀治疗,疗程90天。观察患者治疗前后血瘀证候评分、高敏C反应蛋白(hs-CRP)、血液流变学、颈动脉血流动力学指标和内膜中膜厚度(IMT)变化。结果:治疗组治疗后血瘀证候评分明显减轻,hs-CRP和血液流变学水平明显好转,颈动脉血流动力学和IMT指标明显改善,与对照组比较上述指标也有明显好转(P0.05或P0.01)。相关分析显示IMT与收缩峰期血流速度(PSV)和舒张期末血流速度(EDV)呈负相关,与hs-CRP呈正相关(P0.05)。结论:蛭龙活血方能改善颈动脉粥样硬化并血瘀证患者的临床表现,减缓动脉硬化的进展。     

地龙的鲜品和干品可溶性蛋白及纤溶酶活性的对比研究

目的:对比研究地龙的鲜品和干品的可溶性蛋白及纤溶酶活性.方法:采用Bradfold方法测定蛋白含量,SDS-PAGE进行蛋白谱带的分析,并采用琼脂糖-纤维蛋白平板法进行纤溶酶活性的测定.结果:以干燥品计,鲜品的蛋白提取率为( 13.89±0.86)％,蛋白含量为(63.14±0.38)％,体外纤溶酶活性为(8 743 ±17) IU·g-1;干品蛋白提取率为(9.82±0.75)％,蛋白含量为(45.16 ±0.12)％,体外纤溶酶活性为(206±14) IU·g-1;SDS-PAGE凝胶电泳结果显示,鲜品共显示12条蛋白谱带,干品的谱带较少,且不清晰.结论:鲜地龙的可溶性蛋白提取率、含量及体外纤溶酶活性均明显高于干品,且蛋白组分有较大的差异.     

复方雄黄地龙酊治疗带状疱疹43例临床观察

目的观察复方雄黄地龙酊（OET）治疗带状疱疹的疗效。方法治疗组43例外用OET，而对照组41例外用酞丁安搽剂和1％黄连炉甘石洗剂，两组均静滴阿昔洛韦500mg，每日1次。结果两组在观察指标（止痛、止疱、结痂、痊愈、后遗神经痛发生率）的比较中，差异有统计学意义（P〈0.01或P〈0．05）。结论外用复方雄黄地龙酊治疗带状疱疹，效果良好。     

地龙蛋白多肽类成分的研究进展

动物药地龙药用历史悠久,资源丰富,为临床常用中药之一。现代研究表明蛋白多肽类为地龙主要成分,具有抗凝血、抗脑卒中、抗菌、抗纤维化等多方面的药理作用,已被应用于心脑血管等多种疾病的治疗。对1983年至今发现的48种地龙蛋白多肽类成分的名称、相对分子质量、氨基酸序列、等电点及其活性等进行总结,同时对地龙蛋白多肽类成分的药理作用研究进展进行较全面地综述,以期为地龙蛋白多肽类成分的深入研究与开发利用提供参考。     

水蛭地龙提取液对抗大心肌缺血作用的实验研究

目的观察水蛭地龙提取液的抗心肌缺血作用，为该药治疗心绞痛、心肌梗死等缺血性心脏病提供实验依据。方法采用结扎冠状动脉制作心肌梗死模型，将60只大鼠分为模型对照组（模型组）、假手术组、水蛭地龙提取液治疗组（治疗组），观察各组大鼠治疗后凝血酶时间（TT）、凝血酶原时间（PT）、部分凝血酶原时间（APTT）、D-二聚体（DD）及组织纤溶酶原激活物（t—PA）含量的变化。测定各组大鼠血清中磷酸肌酸激酶（CPK）、乳酸脱氢酶（LDH）、丙二醛（MDA）含量和超氧化物歧化酶（SOD）的活性，并测定心肌梗死范围。结果与假手术组与模型对照组比较，治疗组TT、PT、APTT、明显延长（P〈0．05或P〈0．01），血清CPK、LDH、MDA含量降低（P〈0．05或P〈0．01），且可提高SOD活性，显著减少心肌梗死范围。结论水蛭地龙提取液有抗心肌缺血作用，这一作用与其抗凝促纤溶、心肌保护、改善心肌血液循环有关。     

Pesticide soil contamination mainly affects earthworm male reproductive parameters

Aim: To explore the effect of exposure to commercial Parathion~ (Pc) on the reproductive parameters (sperm and cocoon production and genotoxicity on male germ cells), the survival, the body weight and the gross anatomical changes in Eiseniafoetida. Methods: Three doses of Pc(1478, 739 and 444 mg/kg of soil) and three time intervals of exposure (5, 15 and 30 days) were used. Results: All treated animals were affected. An acute genotoxic effect, revealed by DNA fragmentation (comet assay), was seen by 5 days. Alterations in reproductive parameters were conspicuous in regard to the number of sperm, cocoons and worms born, and the histological observation of the gonads and seminal receptacles. In addition, the body weight and survival rate were decreased. Neuromuscular function was also affected. Conclusion: Earthworms are suitable bioindicators of chemical contamination of the soil, their advantage being their easy and economical handling     

尿激酶或蚓激酶与氟罗沙星联合应用对细菌生物被膜的影响

目的:研究尿激酶(UK) 或蚓激酶(EK) 与氟罗沙星(FLRX) 联合应用对细菌生物被膜的影响。方法:最低抑菌浓度测定采用标准微量稀释法;以银染法快速鉴定细菌生物被膜(BBF) ;扫描电镜(SEM) 观察BBF形态;用甲基四氮唑蓝(MTT) 比色法测定BBF中活菌数。结果:经FLRX处理BBF的SEM图像示细菌形态仍保持短杆状,但细菌数量及菌体间纤维样黏液物质显著减少;经UK或EK与FLRX合用处理后的BBF图像显示细菌破碎、形态不一,菌数和菌体间粘液丝均比单用FLRX时明显减少。对活菌数影响与电镜图像结果一致。结论: UK或EK与FLRX合用对铜绿假单胞菌BBF的抑制具有明显的协同效应。     

Life-History Patterns in Reference and Metal-Exposed Earthworm Populations

The growth and development of F1 Lumbricus rubellus bred from reference and one metal-exposed (smelter) populations were assessed in soils from both sites. In the clean soil, faster growth (as reflected by mean weight) and maturation were found at selected time intervals for the smelter worms. This result is in agreement with predictions from life-cycle models, which indicate that polluted-site populations will be adapted for faster growth, earlier maturation and increased reproductive effort. Life-history adaptation is not however the only explanation for the differences in maturation rates found. During the exposures, mortality of smelter worms was higher than for the reference strain. This resulted in a reduction in the density of the smelter strain. In earthworms, both growth rate and maturation time are known to be density dependent. Thus it is probable that density-mediated responses of growth and development, rather than adaptation, are primarily responsible for the observed life-history.Exposure of the two strains to the smelter site soil was carried out to quantify differences in performance indicative of physiological resistance in the smelter worms. In fact, no consistent differences in growth or maturation were found. Thus it is unlikely that growth and development responses of Lumbricus rubellus are adapted to the metals present in soils at the smelter site.