益智灵对小鼠学习记忆的促进作用

采用一次性训练的被动回避反应—“Ｙ”电迷宫法，观察益智灵对亚硝酸钠和３０％乙醇致小鼠记忆障碍的改善作用，并与尼莫地平和神经生长因子的作用作了比较。３种药物对改善亚硝酸钠致记忆巩固障碍均有效。益智灵和神经生长因子还拮抗３０％乙醇致记忆再现障碍。另结果表明益智灵对正常小鼠学习行为有促进作用，其它两药未见有此作用。     

百岁方颗粒剂对脑梗死患者认知功能障碍的干预作用

目的:探讨百岁方颗粒剂对脑梗死患者认知功能障碍的干预作用.方法:选择脑梗死患者160例,随机分为治疗组80例,对照组80例,分别服用百岁方及尼莫地平,疗程为2个月.观察治疗前后患者的简易智力状态检查表(MMSE)、中医症状、神经功能缺损中国中风量表(CSS)的积分改变及血液流变学、纤维蛋白原等各项指标.结果:临床观察表明,百岁方能提高脑梗死患者MMSE积分,降低中医症状积分,降低CSS积分,改善血液流变学.两组中医症状疗效比较,治疗组总有效率为76.25%,对照组总有效率为58.75%,治疗组明显优于对照组(P＜0.05);治疗组的中医症状积分、CSS积分、血黏度高切均优于对照组(P＜0.01).结论:百岁方颗粒剂是改善脑梗死患者认知功能的有效中药制剂.     

A clinical study of Suogudan Granule in the treatment of rheumatoid arthritis

Objective: To study the clinical efficacy of Suogudan Granule (SGDG) in the treatment of rheumatoid arthritis (RA).Methods: Ninety patients with RA were randomly divided into the treated group and the control group. The treated group was administered orally with SGDG 6 g each time, thrice a day, while the control group with the combined therapy of Fenbid Capsules 0.3 g each time, twice a day and Tripterygium tablet 20 mg each time, thrice a day. The treatment course for both groups was 6 weeks. The changes of clinical symptoms and signs, and laboratory indices such as erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), antistreptolysin O (ASO), routine examination of blood and urine, liver and kidney function, etc. before and after treatment were observed.Results: (1) The total effective rate in the treated group (88. 0%) was obviously higher than that in the control group (67. 5%) with significant difference (P<0.05). (2) The improvement in arthralgia, joint swelling, time of morning stiffness, 15-meter walking, analgesia initiation and persistence in the treated group was better than that in the control group (P<0.05,P<0.01), but there was no obvious difference in improvement of joint tenderness, range of joint motion, grip strength, and initiating detumescence time (P>0.05). (3) The improvement in ESR and RF in the treated group was better than that in the control group with significant difference (P<0.05). The negative conversion rate of ASO in the treated group was also higher than that in the control group (P<0. 01). (4) No evident abnormality in blood, urine, liver or kidney function was found in either group.Conclusion: SGDG is effective and safe for the treatment of RA. Supported by grants from the Bureau of Education of Shaanxi Province(No. 05JK171)     

Cerebellar neuroblastoma

Summary A cerebellar tumor of a 4-month-old girl was examined by light and electron microscopy. The presence of numerous synaptic vesicles in many cell processes and the occasional synaptic devices warranted the diagnosis of neuroblastoma of the cerebellum. The nature of the tumor is discussed briefly.     

Prolonged infusion of cycloheximide does not block mossy fiber sprouting in a model of temporal lobe epilepsy

PURPOSE: The role of protein synthesis in mossy fiber sprouting is unclear. Conflicting reports exist on whether a single dose of the protein synthesis-blocker cycloheximide administered around the time of an epileptogenic injury can block the eventual development of mossy fiber sprouting. METHODS: In rats, osmotic minipumps and cannulae were implanted to deliver 8 mg/ml cycloheximide to one dentate gyrus and vehicle to the other. This method has been used to block protein synthesis in the infused region for up to 5 days with minimal neurotoxic effects (Taha and Stryker, Neuron 2002;34:425-36). After 2 days of infusion, rats were treated with pilocarpine to induce status epilepticus. Pumps were removed 3 days later. Thirty days after pilocarpine treatment, rats were perfused, and hippocampal sections were processed for Timm staining. RESULTS: Timm staining revealed aberrant mossy fiber sprouting in the inner molecular layer regardless of whether hippocampi were treated with cycloheximide or vehicle. Cycloheximide-treated hippocampi displayed more aberrant Timm staining and more tissue damage around the infusion site than did vehicle-treated hippocampi. CONCLUSIONS: Prolonged infusion of cycloheximide, spanning the period of pilocarpine treatment, did not block mossy fiber sprouting. This finding suggests that protein-dependent mechanisms around the time of an epileptogenic injury are not necessary for the eventual development of synaptic reorganization.     

Properties of GABA(A) receptor-mediated transmission at newly formed Golgi-granule cell synapses in the cerebellum

Cerebellar granule cells receive inhibitory synaptic input from Golgi cells, which is mediated by gamma-aminobutyric acid (GABA) acting on GABA(A) receptors. In the present study we examined the properties of GABAergic inhibitory postsynaptic currents (IPSCs) in granule cells of the rat at a time when they first receive synaptic contacts from Golgi cells. Our results demonstrate that granule cells receive functional GABAergic synaptic input as early as postnatal day three (P3). The kinetic properties of these early IPSCs and the single-channel conductance of the synaptic receptors are similar to those seen at the end of the first postnatal week, suggesting a stable subunit composition during this initial period of development. However, at P3, unlike the situation at more mature synapses, two distinct patterns of synaptic activity are evident, with IPSCs occurring either regularly or in bursts. In addition we find that Golgi cells are spontaneously active during early development, and at P7 most IPSCs are action potential-dependent. Moreover, paired Golgi-granule cell recordings suggest a high level of connectivity and a high release probability at these early synapses.     

桃花止泻冲剂的药效学研究

采用炭末推进法和药物性腹泻模型,观察了桃花止泻冲剂对小鼠小肠推进运动、药物性腹泻的影响,并初步观察桃花止泻冲剂的抗炎作用。结果表明,桃花止泻冲剂能明显抑制新斯的明引起的小鼠小肠推进亢进作用,减少番泻叶引起的小鼠腹泻发生率和次数,并具有剂量依赖性。桃花止泻冲剂11．70和17．55g／kg剂量组能明显抑制乙酸引起小鼠腹腔毛细血管通透性的增高。     

康安冲剂抗衰老作用的基础研究

目的 :通过动物实验考察康安冲剂的抗衰老作用 ;方法 :观察康安冲剂对老化代谢产物及相关酶活性、总胆固醇、甘油三酯胆固醇含量的影响 ;结果及结论 :康安冲剂对青年、老年小鼠的过氧化脂质产物MDA含量均有明显降低作用 ,对小鼠脑和肝MAO -B活性具明显抑制作用 ,并明显提高脑和肝SOD活性 ,与同龄对照组比较P <0 0 1。并且具有促进蛋白质合成及促性激素样作用。康安冲剂可明显降低高脂血症大鼠总胆固醇、甘油三酯胆固醇、低密度脂蛋白的含量并对高密度脂蛋白的含量具有提升作用。     

金诃愈心冲剂防治高脂血症的实验研究

为研究金诃愈心冲剂(主药：白檀香、广酸枣、肉豆蔻、藏锦鸡儿等)治疗心血管疾病的机理,给予Wistar大鼠灌喂高脂饲料的同时,以愈心冲剂灌胃,8周后测定血脂和血液流变学指标,并与脂必妥组比较。结果：金诃愈心冲剂能防止高脂饲料所致的血脂和血液流变性的紊乱,且有一定的量效关系。提示金诃愈心冲剂能防治高脂血症,这可能是该药治疗心血管疾病的机理之一。     

平喘冲剂治疗小儿哮喘临床观察

为观察平喘冲剂治疗小儿哮喘的临床疗效,将196例患儿随机分为治疗组100例口服中药平喘冲剂,对照组96例静脉滴注青霉素、氨茶碱,疗程1周,观察治疗前后喘憋、咳嗽、紫绀、哮鸣音、肺功能、肺阻抗血流的变化。结果：治疗组总有效率为98．00％,对照组总有效率为93．75％,各项检测指标治疗组均优于对照组。提示平喘冲剂具有平喘止咳、化痰通瘀的作用。