中药解郁胶囊治疗老年抑郁症的临床疗效观察

目的：观察中药解郁胶囊对老年抑郁症的临床疗效和安全性。方法：选取2020年11月至2021年12月甘肃中医药大学附属医院住院或门诊患者200例作为研究对象,按照纳入标准最终产生研究观察对象100例,健康对照组来自我院组织的年龄在60~80岁的健康体检的老年人100例。观察组中男37例,女63例,对照组中男43例,女57例。对照组采取常规治疗,观察组采取中医综合疗法治疗。对2组患者治疗前后减分情况、中医证候分析、服药及依从情况、不良反应及抑郁焦虑因子等无创性指标的影响。结果：对老年抑郁症的治疗效果主要从汉密尔顿抑郁量表减分情况及证候减分率体现,2组经过12个月中药和抗抑郁综合治疗,中医证候量表减分率（8.2±3.1）和（7.8±3.2）,比对照组的（8.8±3）与（8.4±3.6）,P值为0.001 6和0.001 3,治疗前后均有明显变化,差异有统计学意义（P<0.05）,中医证候量表减分率比较,差异有统计学意义（P<0.05）,从服药后出现的不良反应来看,中医综合观察组应明显低于对照观察组,差异有统计学意义（P<0.05）。从中医组治疗患者的依从性分析,差异有统计学意义（P<0.05）。结论：中药解郁胶囊与中医综合治疗患者治疗效果显著,依从性更佳。     

Neuroprotective effects of Longxue Tongluo Capsule on ischemic stroke rats revealed by LC-MS/MS-based metabolomics approach

Objective: The present study aimed to evaluate the therapeutic effect and explore the underlying mechanisms of Longxue Tongluo Capsule (LTC) on ischemic stroke rats. Methods: Twenty-six rats were randomly divided into four groups, including sham group, sham + LTC group, MCAO group, and MCAO + LTC group. Ischemic stroke rats were simulated by middle cerebral artery occlusion (MCAO), and LTC treatment group were orally administrated with 300 mg/kg of LTC once daily for seven consecutive days. LTC therapy was validated in terms of neurobehavioral abnormality evaluation, cerebral infarct area, and histological assessments. The plasma metabolome comparisons amongst different groups were conducted by UHPLC-Q Exactive MS in combination with subsequent multivariate statistical analysis, aiming to finding the molecules in respond to the surgery or LTC treatment. Results: Intragastric administration of LTC significantly decreased not only the neurobehavioral abnormality scores but also the cerebral infarct area of MCAO rats. The interstitial edema, atrophy, and pyknosis of glial and neuronal cells occurred in the infarcted area, core area, and marginal area of cerebral cortex were improved after LTC treatment. A total of 13 potential biomarkers were observed, and Youden index of 11 biomarkers such as LysoPC, SM, and PE were more than 0.7, which were involved in neuroprotective process. The correlation and pathway analysis showed that LTC was beneficial to ischemic stroke rats via regulating glycerophospholipid and sphingolipid metabolism, together with nicotinate and nicotinamide metabolism. Heatmap and ternary analysis indicated the synergistic effect of carbohydrates and lipids may be induced by flavonoid intake from LTC. Conclusion: The present study could provide evidence that metabolomics, as systematic approach, revealed its capacity to evaluate the holistic efficacy of TCM, and investigate the molecular mechanism underlying the clinical treatment of LTC on ischemic stroke.     

基于BDNF/TrkB/p-CREB信号通路探讨血府逐瘀胶囊促进神经再生防治卒中后抑郁的作用及机制

目的 探讨血府逐瘀胶囊对卒中后抑郁（post-stroke depression,PSD）大鼠抑郁症状的改善作用,并基于神经再生关键通路脑源性神经营养因子（brain derived neurotrophic factor,BDNF）/酪氨酸激酶受体B(tyrosine kinase receptor,TrkB)/磷酸化环腺苷酸应答元件结合蛋白（phosphorylated cAMP response element-binding protein,p-CREB）探讨其机制。方法 采用短暂大脑中动脉梗塞（transient middle cerebral artery occlusion,tMCAO）复合慢性不可预知应激（chronic unpredictable mild stimulation,CUMS）方法复制PSD大鼠模型。大鼠随机分为假手术组、模型组及血府逐瘀低、高剂量（0.43、0.86 g/kg）组和氟西汀（1.8 mg/kg）组,每组18只。连续给予药物干预28 d,利用神经功能评分、糖水偏好实验、旷场实验、强迫游泳实验考察血府逐瘀胶囊对PSD大鼠神经功能损伤以及抑郁症...     

痹祺胶囊治疗类风湿性关节炎的药效研究及网络机制预测

目的 基于Ⅱ型胶原诱导的类风湿性关节炎（rheumatoid arthritis,RA）大鼠模型和网络药理学方法探讨痹祺胶囊的药效作用及作用机制。方法 SD大鼠采用Ⅱ型胶原蛋白造模,造模成功大鼠随机分为模型组,痹祺胶囊（0.05、0.10、0.20、0.40 g/kg）组及泼尼松（10 mg/kg）组和雷公藤总苷（10 mg/kg）组,连续给药15 d。持续监测大鼠体质量、足趾肿胀度和关节炎评分,苏木素-伊红染色考察大鼠踝关节病理变化,ELISA法测定血清细胞因子含量,初步评价痹祺胶囊治疗效果。选择痹祺胶囊中39个化合物为研究对象,依据反向药效团匹配方法和TCMSP、Uniprot等数据库预测化合物作用靶点,与通过OMIM、Dis Ge Net等数据库收集的RA相关靶点相互交互,将交互靶点借助Omicsbean、STRING等数据库平台对获得靶点进行基因本体功能和京都基因与基因组百科全书（Kyoto encyclopedia of genes and genomes,KEGG）通路富集分析,利用Cytoscape软件构建“药材-化合物-靶点-通路-功效-疾病”网络,预测其可能的作用机制...     

来曲唑联合复方玄驹胶囊治疗肥胖性少弱精子症的临床疗效观察

目的观察来曲唑、枸橼酸氯米芬分别联合复方玄驹胶囊及维生素E治疗肥胖性少弱精子症的临床疗效。方法选取2018年8月至2020年8月在洛阳市妇幼保健院生殖医学研究所就诊的188例肾阳不足型肥胖性少弱精子症患者,随机分为来曲唑联合组(A组)和枸橼酸氯米芬联合组(B组)各94例,疗程为3个月,以精子浓度、精子前向运动百分率和生殖激素(FSH、LH、E 2、T)水平为观察指标进行治疗前、后疗效评估。结果精液质量上,治疗前,两组患者的精子浓度和前向运动百分率均无显著性差异;治疗3个月后,两组患者均有显著提升,且A组患者的精子浓度[(26.32±2.59)×10⁶/ml vs.(21.48±3.37)×10⁶/ml]显著高于B组,即A组精子浓度增加更加显著(P<0.05)。生殖激素水平的比较:与治疗前相比,治疗后A组患者的LH[(5.31±2.24)U/L vs.(3.84±1.47)U/L]及T[(1.73±0.06)nmol/L vs.(1.12±0.05)nmol/L]水平显著升高(P<0.05),E 2水平[(152.16±55.89)pmol/L vs.(206.69±60.44)pmol/L]显著下降(P<0.05),T/E 2比值显著升高(P<0.05);B组中仅有T水平[(1.53±0.07)nmol/L vs.(1.17±0.06)nmol/L]显著改善,且A组E 2水平与T/E 2比值显著高于B组(P<0.05)。结论对于肾阳不足型肥胖性少、弱精子症,尤其是高E 2而T/E 2值相对较低的患者,以来曲唑为主效药物的联合用药在提高精子数量、改善精子活力方面具有显著效果,值得临床推广。     

通脉活血灵胶囊对实验性高脂血症家兔血脂及动脉粥样硬化的影响

观察通脉活血灵胶囊对实验性高脂血症家兔血脂调节变化和动脉粥样经形成的影响。实验结果表明,高脂饲料喂养家兔可导致高脂血症,并可诱发动脉弱样硬化。     

复方薤白滴丸和复方薤白胶囊的药代动力学

目的 考察自制的复方薤白滴丸在家兔体内的动态行为,并与复方薤白胶囊剂进行比较。方法 家兔单剂量口服给药后,以小檗碱为指标,用ＨＰＬＣ法测定在不同时间点的血药浓度。结果 复方薤白滴丸的主要药动学参数：Ｃｍａｘ１５４．３μｇ／Ｌ,ＭＡＴ７．８ｈ,ＡＵＣ０→∞４２８．０μｇ／Ｌ;复方薤白胶囊的主要药动学参数：Ｃｍａｘ８９．８μｇ／Ｌ,ＭＡＴ１３．８ｈ,ＡＵＣ０→∞３９８．５μｇ／Ｌ。结论 复方薤白滴丸较     

正交试验法优选银翘解毒软胶囊的水煮提取工艺

采用Ｌ９（３＾４）正交设计,以浸膏得率,正丁醇浸出率,绿原酸含量为考察指标,优选银翘解毒软胶囊的水煮提取工艺。结果表明：最佳提取工艺为首次加１０倍量水,浸泡３０ｍｉｎ,煎煮２ｈ,第２次加８倍量水,煎煮１．５ｈ。     

晶珠肝泰舒胶囊治疗肝炎肝硬变60例

观察了服用晶珠肝泰舒胶囊治疗慢性肝炎 ５２例、肝炎后肝硬化（代偿期） ８例。经 ３个月（ １疗程）治疗,全部病例临床症状、体征与肝功改善均较显著,显效率达８３ ３３％。其中对肝炎后肝硬变作用明显,提示晶珠肝泰舒胶囊在保肝同时,还有一定抗肝纤维化作用。对乙肝病毒血清标志物ＨＢＶ－ＤＮＡ阴转率１２ ５％,ＨＢｓＡｇ阴转率１５．７８％,ＨＢｃＡｇ转阴率７ ４％,提示晶珠肝泰舒胶囊具有抗病毒作用。     

“生精胶囊”对大鼠的生精作用的研究

本文用病理学的计数方法研究了“生精胶囊”对大鼠的生精作用。结果表明“生精胶囊”能明显增加睾丸曲细精管内生殖细胞数目、睾丸内精子数目、曲细精管数量及其直径和曲细精管内细胞层数,增加精子活率而对初级精母细胞直径及精子活动力无明显影响。为临床该药治疗“男性不育少精症”提供了科学依据。