浅析仲景附子疗痛类方

附子治疗痛类方中附子所治之痛,皆阳虚阴凝为患,附子补五脏之阳、壮命门之火,阳振阴散,其痛自止,配伍不同类药物,以达到针对主症,旁疗兼症之效。力求一药多能,综合发挥作用;重视人体生理,除邪先护正,祛邪留途径。     

银尔通含漱联合地塞米松、利多卡因雾化吸入治疗鼻咽癌放疗所致咽炎的疗效

目的:探讨银尔通含漱联合地塞米松、利多卡因雾化吸入治疗鼻咽癌放疗性咽炎的临床效果。方法:将2011年11月～2012年6月本院住院的64例鼻咽癌放疗所致咽炎患者随机分为观察组和对照组各33例,观察组以银尔通含漱联合地塞米松、利多卡因雾化吸入治疗,对照组以生理盐水含漱联合地塞米松、利多卡因雾化吸入治疗,比较两组疗效。结果:观察组治疗总有效率高于对照组。结论:银尔通含漱联合地塞米松、利多卡因雾化吸入治疗鼻咽癌放疗所致咽炎临床效果好,使用安全,值得临床推广应用。     

脉通口服液质量标准的研究

目的：制定脉通口服液（葛根,丹参）的质量标准。方法：用TCL鉴别制剂中葛根、丹参;采用HPLC法测定葛根素的含量。结果：TLC色谱中均能明显的检出葛根、丹参;HPLC法测得葛根素含量2.2920mg/ml,平均回收率为99.14％,RSD为1.51％（n=5）。定性、定量方法简便,准确,专属性强。结论：建立的方法能有效地控制脉通口服液的质量。     

筋脉通对糖尿病大鼠坐骨神经NADPH氧化酶p22-phox亚基表达的影响

目的观察中药筋脉通对糖尿病大鼠坐骨神经NADPH氧化酶p22-phox亚基表达的影响。方法将STZ诱导的糖尿病大鼠随机分为模型组,维生素C组,筋脉通小、中、大剂量组,并设立正常对照组,连续灌胃16周。检测各组治疗前及治疗后4、8、12、16周的体重、血糖;测定第16周时大鼠机械痛阈值;采用免疫组化法测定大鼠坐骨神经NADPH氧化酶p22-phox亚基的表达,并进行半定量分析。结果与正常组相比,各组糖尿病大鼠体重均显著下降(P<0.01),血糖均明显升高(P<0.01);与模型组比较,各治疗组大鼠体重及血糖在各时间点均无显著差异(P>0.05)。与正常组相比,模型组、VC组、筋脉通小、大剂量组机械痛阈值显著降低(P<0.01);各治疗组机械痛阈值较模型组均明显升高(P<0.01);与VC组比较,筋脉通中剂量组机械痛阈值显著升高(P<0.01)。与正常组相比,模型组及各治疗组p22亚基的IOD值明显升高(P<0.05,P<0.01);各治疗组p22亚基的IOD值较模型组均显著降低(P<0.01);与VC组比较,筋脉通中、大剂量组p22亚基IOD值显著降低(P<0.01,P<0.05)。结论筋脉通能显著降低大鼠坐骨神经NADPH氧化酶p22-phox亚基的表达。     

仝小林教授运用干姜黄芩黄连人参汤治疗2型糖尿病辨证要点分析

仝小林教授善用干姜黄芩黄连人参汤治疗2型糖尿病中辨证属"脾虚胃热"者。本研究采用描述的统计方法,分析仝小林教授运用该方治疗2型糖尿病患者319人次的证候特点,初步探讨本方治疗2型糖尿病的辨证要点。     

玉龙散结合趣络宝治疗膝骨性关节炎的效果及护理

目的探讨玉龙散结合熥络宝治疗膝骨性关节炎的临床效果。方法选取住院患者60例随机分为观察组和对照组各30例,观察组采用玉龙散结合魉络宝治疗,对照组采用玉龙散直接外敷,比较两组骨关节炎指数评分（WOMAC）、皮肤刺激反应及疗效。结果治疗前两组WOMAC各项评分比较,差异均无统计学意义（P〉0．05）,治疗后观察组WOMAC疼痛、肿胀、晨僵指标的得分分别为（0．93±0,63）,（0．93±0．49）,（0．93±0．52）分均低于对照组的（1．16±0．74）,（1．06±0．48）,（1．16±0．59）分,差异均有统计学意义（t分别为2．90,3．24,2．16;P〈0．05）;观察组皮肤刺激发生率为10．0％,低于对照组的33．3％,差异具有统计学意义（X^2=4．81,P〈0．05）;治疗效果方面观察组优于对照组,差异有统计学意义（X^2=5．45,P〈0．05）。结论玉龙散结合魑络宝治疗膝骨性关节炎优于玉龙散直接外敷,效果好,方法操作简单,可反复使用。     

Interleukin-34 drives macrophage polarization to the M2 phenotype in autoimmune hepatitis

BackgroundAutoimmune hepatitis is a chronic inflammatory disease, the abnormal immunological function is the main pathogenesis. Interleukin-34 is a newly identified cytokine that shares the same receptor as colony stimulating factor-1.MethodsWe used interleukin-34 knockout and wild-type mice in a Con A-induced hepatitis model and cocultured RAW264.7 macrophage cells with interleukin-34. We then detected associated inflammatory cytokine and chemokine levels to elucidate the role of interleukin-34.ResultsIn this study, we found that the loss of interleukin-34 resulted in higher sensitivity to Con A-induced hepatitis. RAW264.7 macrophage cells were able to differentiate to the M2 phenotype upon interleukin-34 stimulation.ConclusionsWe conclude that interleukin-34 may protect the liver from Con A-mediated hepatitis by driving M2 macrophage polarization and suppressing inflammation.     

夏枯草、黄芩、钩藤治疗肝热型高血压病经验--仝小林三味小方撷萃

仝小林教授将高血压病分为"三期六态",指出热壅态中的肝热证具有"四红二干"的临床表现,"四红"指面(目)红、唇红、舌红、掌红,"二干"指便干、口干。常处以靶方夏枯草、黄芩、钩藤清肝降压,一般用量为夏枯草30~60 g,黄芩15~30 g,钩藤15~30 g,根据兼证配合使用清热、降火、散火、行气、利湿之品。该小方治疗肝热型高血压病时,降压效果显著,并可以明显减轻头晕、头痛、目赤等症状,临床疗效良好,但需注意不可长期大剂量使用。     

Expression of co-inhibitory molecules B7-H4 and B7-H1 in Epstein-Barr virus positive diffuse large B-cell lymphoma and their roles in tumor invasion

To investigate the relationship between immunoregulatory molecules B7-H4 and B7-H1 in Epstein-Barr positive diffuse large B-cell lymphoma (EBV⁺DLBCL). Immunohistochemistry was used to detect the expression of B7-H4 and B7-H1 in tumor tissues of 13 patients with EBV⁺DLBCL. The expression levels of B7-H4 and B7-H1 in four diffuse large B-cell lymphoma cell lines (SU-DHL-4, SU-DHL-10, SU-DHL-6, Pfeiffer) were analyzed by flow cytometry. Transwell invasion assays were conducted to observe the invasive ability of cell lines. B7-H4 and B7-H1 were expressed in 84.62% and 100% tumor specimens of EBV⁺DLBCL. The overexpression of B7-H4 and B7-H1 was found in 46.15% and 23.08% tumor samples of EBV⁺DLBCL. There was a medium negative correlation between the expression levels of B7-H4 and B7-H1 (r = -0.667, P = 0.013, spearman rank correlation). The expression levels of B7-H1 in four diffuse large B-cell lymphoma cell lines were positively correlated with their invasive ability, whereas the expression levels of B7-H4 were not. Here, we provide evidence for the negative relationship between B7-H4 and B7-H1 in EBV⁺DLBCL. The expression of B7-H1 in EBV⁺DLBCL appears to be the dominant factor which affects tumor aggressiveness. When B7-H1 expression weakens, the molecule B7-H4 may become the dominant factor of prognosis in patients with EBV⁺DLBCL.