川芎嗪对P2X3受体介导的大鼠伤害性兴奋传入的作用

通过动物痛行为反应(缩足反射)确定局部和鞘内应用川芎嗪(TMP)对ATP等P2X受体激动剂所致大鼠足底急性伤害性行为反应的影响。P2X3受体拮抗剂TNP-ATP(0．3μmol／L)明显抑制P2X受体激动剂ATP(1μmol／L)或α，β-meATP(0．6μmol／L)引起的大鼠足底急性伤害性反应。大鼠足底局部应用TMP(0．1-10mmol／L)剂量依赖性地对ATP(1μmol／L)或α，β-meATP(0．6μmol／L)引起的伤害性反应具有抑制作用。鞘内应用TMP(50mmol／L)对ATP(1μmol／L)或α，β-meATP(0．6μmol／L)引起的伤害性反应具有抑制作用。结果表明，TMP可通过阻断P2X3受体介导的伤害性兴奋传入抑制P2X受体激动剂引起的大鼠足底急性伤害性反应。     

大学生篮球运动员集训期间部分淋巴细胞亚群和Th1/Th2细胞因子mRNA表达变化分析

目的:探讨大负荷体能训练对大学生篮球运动员细胞免疫功能的影响。方法:14名男子大学生篮球运动员进行为期16周的集训,在训练期间分次对运动员外周血淋巴细胞亚群(CD3+、CD4+/CD8+淋巴细胞比值、NK细胞比例)和Th1/Th2细胞因子 mRNA表达进行检测。结果:在两次分别持续了6周的训练期后,运动员外周血CD3+淋巴细胞数于增加后出现显著下降,从第7周的峰值(59.74±7.18)下降至第16周的最低值(52.02±7.92)(P<0.001);CD8+细胞数第16周(31.15±6.25)与第3周(37.98±7.05)相比显著下降(P<0.05);NK细胞数从第7周的最高值(19.62±5.21)下降至第14周的最低值(14.41±7.93)(P<0.05);IL-4 mRNA表达训练后与基础值(6.56±0.71)相比显著增加,从第7周的7.04±0.35和第14周的7.30±0.25,直至第16周达到最大值7.36±0.45(P<0.05),其他指标的变化不具有统计学意义。结果提示,大负荷训练使运动员细胞免疫功能削弱,Th1/Th2平衡向Th2方向漂移。     

三芪丹颗粒治疗2型糖尿病合并高血压病的临床观察

目的 评价益气养阴、活血化瘀法对2型糖尿病（T2DM）合并高血压病患者胰岛素抵抗的影响。     

胰抗消胶囊干预2型糖尿病胰岛素抵抗的临床研究

目的观察胰抗消胶囊对2型糖尿病胰岛素抵抗的影响。方法将80例2型糖尿病患者随机分为治疗组46例和对照组34例，2组均予盐酸二甲双胍治疗口服，治疗组在此基础上予胰抗消胶囊口服。2组均8周为1个疗程。治疗前后分别测空腹血糖（FPC）、总胆固醇（TC）、甘油三酯（TG）、糖化血红蛋白（HbAlc）、空腹胰岛素（FINS）、胰岛素敏感性指数（ISI）。结果治疗组总有效率为93．48％，对照组总有效率为67．65％，2组比较差异有统计学意义（P〈0．05），治疗组优于对照组；治疗组治疗后FPG、TC、TG、HbAlc、FINS、ISI水平与治疗前比较差异有统计学意义（P〈0．05），对照组FPG、HbAlc、FINS、ISI水平与治疗前比较差异有统计学意义（P〈0．05）；治疗后2组间比较，治疗组TC、TG、FINS、ISI水平与对照组比较差异有统计学意义（P〈0．05），治疗组优于对照组。结论胰抗消胶囊能明显改善2型糖尿病的胰岛素抵抗。     

过敏康Ⅱ号胶囊对AsAb阳性大鼠睾丸Bcl-2、Bax表达影响的实验研究

目的观察过敏康Ⅱ号胶囊对AsAb阳性大鼠睾丸Bcl-2、Bax表达的影响。方法选取健康成年雄性SD大鼠60只，按体重随机分为正常组，模型组，对照组，高、中、低剂量组，每组10只。采用主动免疫法建立血清抗精子抗体（AsAb）阳性动物模型10只，灌胃给药，免疫组化方法观察药物对AsAb阳性大鼠睾丸Bcl-2、Bax表达的影响。结果过敏康Ⅱ号高剂量组睾丸生精细胞和精子Bcl-2表达的平均吸光值显著高于模型组（P〈0．01），而Bax表达的平均吸光值显著低于模型组（P〈0．01）。结论调节睾丸Bcl-2、Bax的表达是过敏康Ⅱ号清除或抑制AsAb起治疗作用的机制之一。     

Following Anti-CD25 Treatment, A Functional CD4+CD25+ Regulatory T-Cell Pool Is Present in Renal Transplant Recipients

Daclizumab, a humanized antibody directed against the alpha-chain of the interleukin-2 receptor (CD25), has shown efficacy in the prevention of acute rejection following organ transplantation. However, anti-CD25 therapy can be expected to affect not only alloreactive effector T cells, but also CD4(+)CD25(+) regulatory T (Treg) cells that are shown to play an important role in the induction of transplantation tolerance. Therefore, the size and function of the Treg pool in human renal allograft recipients after single-dose daclizumab administration was investigated in this study. Approximately 8 weeks after administration, daclizumab was cleared from the circulation and the Treg population then present appeared not different from that observed before transplantation. Functional analysis revealed that the Treg possessed a normal capacity to suppress mixed lymphocyte reactions in vitro. These data indicate that after daclizumab therapy a Treg population, normal in number and function, is present in the peripheral blood of renal transplant recipients.     

实验性2型糖尿病大鼠模型的建立和评价(Ⅰ)——体重、血糖和肝糖原的变化

目的：用高脂饲料加小剂量STZ腹腔注射建立实验性2型糖尿病大鼠模型。方法：采用Wistar大鼠,分为正常对照组、高脂饲料组、小剂量STZ组（30 mg/kg）和高脂饲料加小剂量STZ（30 mg/kg）组,每10 d测定大鼠体重、空腹血糖和肝糖原等指标,连续50 d,对各组大鼠上述指标进行比较和评价。结果：①体重：高脂饲料组大鼠体重呈连续上升趋势,小剂量STZ组和高脂饲料加小剂量STZ组大鼠体重呈先降低再恢复的趋势;②空腹血糖：高脂饲料组大鼠空腹血糖轻微升高,小剂量STZ组大鼠空腹血糖呈上升的趋势,高脂饲料加小剂量STZ组大鼠空腹血糖呈明显上升趋势;③肝糖原：高脂饲料组大鼠肝糖原明显升高,小剂量STZ组、高脂饲料加小剂量STZ组大鼠肝糖原未见明显改变;结论：高脂饲料加小剂量STZ腹腔注射能成功诱导2型糖尿病大鼠模型,这种造模方法具有成模后血糖维持在较高水平、自身缓解较少、症状较轻和动物状态良好等优点。     

Changes in microtubule-associated protein-2 (MAP2) expression during development and after status epilepticus in the immature rat hippocampus

In this study, we analyzed the spatiotemporal expression patterns of the high-molecular weight (MAP2a and b) and low-molecular weight (MAP2c and d) cytoskeletal microtubule-associated protein-2 (MAP2) isoforms with Western blotting, and the cellular localization of the high-molecular weight MAP2 isoforms with immunocytochemistry in the hippocampi of 1- to 21-day-old rats. Moreover, the temporal profile (from 30 min to 1 week) of MAP2 isoform reactivity to kainic acid-induced status epilepticus was studied in P9 rats. During development, the expression of the high-molecular weight MAP2 isoforms significantly increased, while the low-molecular weight isoforms decreased, the most prominent changes occurring during the second postnatal week. This developmental increase in the high-molecular weight MAP2 expression was also confirmed with immunocytochemistry, which showed increased immunoreactivity, particularly in the molecular layers of the dentate gyrus, and in CA1 and CA3 stratum radiatum. In 9-day-old rats, status epilepticus resulted in a rapid transient increase (about 210%) in the high-molecular weight MAP2 expression, without any effect on the low-molecular weight MAP2. Moreover, disturbed dendritic structure in the CA1 and CA3 stratum radiatum was manifested as formation of varicosities 3h after the kainic acid treatment. The strictly developmentally regulated MAP2 isoform expression suggests different functional roles for these proteins during the postnatal development in the rat hippocampus. Moreover, high-molecular weight MAP2s may play a role in nerve cell survival during cell stress.     

Screening for SNCA and LRRK2 mutations in Greek sporadic and autosomal dominant Parkinson's disease: identification of two novel LRRK2 variants

Mutations in SNCA and LRRK2 genes, encoding alpha-synuclein and leucine-rich repeat kinase 2, respectively, cause autosomal dominant Parkinson's disease (AdPD). The LRRK2 G2019S (c.6055G > A) and R1441G (c.4321C > G) mutations have also been identified in sporadic PD (sPD). We studied 55 unrelated patients with AdPD, 235 patients with sPD, and 235 healthy age- and gender-matched controls all of Greek origin. Patients with AdPD were screened for SNCA and LRRK2 mutations by direct sequencing. SNCA gene dosage analysis was also performed for AdPD using quantitative duplex polymerase chain reaction of genomic DNA. In addition, we investigated the frequency of the LRRK2 G2019S mutation in sPD. We found no missense mutations or multiplications in the SNCA gene. Here we report two novel variants, A211V (c.632C > T) and K544E (c.1630A > G) in LRRK2 gene in two patients with AdPD that was not present in controls. We identified only one patient with sPD (1/235; 0.4%) carrying the G2019S mutation. LRRK2 mutations are present in AdPD and sPD patients of Greek origin.     

低血糖指数食物饮食教育对2型糖尿病患者血糖的影响

目的观察低血糖指数食物饮食教育对2型糖尿病患者血糖的影响。方法将90例2型糖尿病患者随机分为对照组和观察组各45例。观察组采用低血糖指数食物饮食教育，对照组给予常规饮食教育，两组均随访观察4周。结果干预后对照组与观察组空腹血糖、餐后2h血糖比较，差异有显著性意义（均P〈O．01）。结论低血糖指数食物饮食教育有助于2型糖尿病患者提高饮食依从性和平稳控制血糖。