灵芝孢子对2型糖尿病大鼠肾脏Cyt-C和线粒体钙的影响

目的观察灵芝孢子对2型糖尿病大鼠肾脏组织细胞色素C（Cyt-C）及线粒体钙的影响。方法将清洁级Wistar大鼠50只。雌雄各半，随机分成3组（正常组、模型组、灵芝孢子组）。模型组及灵芝组以25mg／kg的剂量一次性腹腔注射链尿佐菌素（STZ），正常对照组腹腔注射等量柠檬酸钠一柠檬酸缓冲液。正常饮食喂养2wk后，进行糖耐量试验，模型组和灵芝组以糖耐量异常者保留，并改喂高脂高糖饮食，灵芝组另加灵芝孢子（250mg／ks／d）持续10wK。动物均单独喂养，实验结束前一天做糖耐量试验，取肾脏检测肾脏组织胞色素C、线粒体钙的水平。结果模型组肾脏组织线粒体Cyt-C、线粒体钙均显著低于对照组和灵芝孢子粉组（P〈0．05），胞质Cyt-C显著高于对照组和灵芝孢子粉组（P〈0．05）。结论灵芝孢子对糖尿病大鼠肾脏组织具有保护作用。     

灵芝多糖对肿瘤细胞与内皮细胞相互作用的影响

目的研究灵芝多糖(GlPS)抗肿瘤作用及其机制。方法通过相差显微镜和间接免疫荧光的方法鉴定了人脐静脉内皮细胞(HUVECs)。采用MTT法检测GlPS对人前列腺癌细胞(PC-3M)和HUVECs增殖的影响。检测GlPS对PC-3M细胞与HUVECs粘附的影响。采用transwell双层小室观察GlPS对PC-3M迁移穿过单层HUVECs的影响。结果GlPS对PC-3M无直接细胞毒作用,对HUVECs增殖无显著性作用。GlPS能够减少粘附和迁移穿过单层内皮细胞的肿瘤细胞数目。结论灵芝多糖通过抑制肿瘤细胞粘附并迁移穿过内皮细胞发挥其抗肿瘤作用。     

女贞子的X射线衍射Fourier指纹图谱鉴定

目的:建立中药材女贞子新鉴定分析方法。方法:以乙醇、氯仿为提取溶剂提取中药材女贞子,采用X射线衍射Fourier谱鉴定法。结果:通过对四个中药材女贞子的乙醇、氯仿提取物实验分析比较,获得中药材女贞子的标准X射线衍射Fourier图谱及特征标记峰值。结论:X射线衍射Fourier谱鉴定法可用于中药材女贞子的分析鉴定。     

灵芝多糖引起小鼠腹腔巨噬细胞膜超极化

用激光扫描共聚焦显微 镜（ Ｌ Ｓ Ｃ Ｍ） 技 术,动态 监测了 灵芝多 糖 Ｇ Ｌ Ｐ 均 一体组 分 Ｇ Ｌ Ｂ７ 对小 鼠腹腔巨噬细胞（ ＭΦ） 膜电位（ Ｍ Ｐ） 的影 响,结 果 Ｇ Ｌ Ｂ７ 能 引起 小 鼠腹 腔 ＭΦ 膜快 速超 极化, 最 低降 低幅 度为 ３９ ±９ ％ ,之后慢慢复极,但在１０ ｍｉｎ 内未恢复至原来水平。提示灵芝多糖引起小鼠腹腔 ＭΦ 膜超极化是其信 号跨膜转导的重要机制。     

用二维核磁共振技术研究赤芝孢子内酯A和B的结构

通过~1H-~1H,~1H-~(13)C相关谱(COSY),~1H-~(13)C远程偶合相关谱(OOLOC)及NOE二维谱(NOESY)等新的二维核磁共振技术确定了两个新化合物赤芝孢子内酯A和B的结构。     

复方灵芝汤联合阿德福韦酯治疗HBeAg阳性慢性乙型肝炎临床研究

目的：观察复方灵芝汤联合阿德福韦酯治疗病毒性肝炎的临床疗效。方法：将108例慢性乙型病毒性肝炎患者随机分为两组,对照组单纯采用阿德福韦酯胶囊10 mg口服,1次/d,疗程2 a。观察组在对照组基础上加用复方灵芝汤并随症加减治疗,0.5 a后用药同对照组。结果与结论：复方灵芝汤联合阿德福韦酯治疗慢性乙型病毒性肝炎可以提高疗效,且疗效稳定。     

Influence of Ganoderma lucidum spores on the levels of neuropeptide Y and somatostatin in brains of seizure rats

BACKGROUND: Previous research has revealed that somatostatin can induce epilepsy, and that the levels of neuropeptide Y may increase and become more active in brain areas with epileptic seizures.OBJECTIVE: To observe the effect of Ganoderma lucidum spore powder on the neuropeptide Y and somatostatin content in the cerebral cortex and hippocampal regions of seizure rats induced by pentylenetetrazol (PTZ). Furthermore, to verify any effect of Ganoderma lucidum spore powder on inhibition of epileptic seizures.DESIGN, TIME AND SETTING: A randomized group animal study was performed in August 2007 in the School of Basic Medical Sciences, Jiamusi University (Jiamusi, Heilongjiang, China).MATERIALS: Thirty healthy, male, Wistar rats, aged 12 weeks and weighing 180-220g, were taken as the experimental animals. PTZ (Sigma Company, United States) was used to induce epilepsy. Ganoderma lucidum spores (Leyss, ex Fr variety) were purchased from Jiamusi City Wild Growing Case of the Ganoderma Lucidum (China). Rabbit anti-somatostatin antibodies and secondary antibodies were purchased from Wuhan Boster Company (China). Neuropeptide Y radioimmunoassay kit was purchased from Beijing Furui Biotechnology Company (China).METHODS: Thirty rats were randomly divided into three groups: a control group, an epilepsy model group and a Ganoderma lucidum spore-treated group. Each group contained 10 animals. Rats in the epilepsy model group were treated with intraperitoneal injections of PTZ and gastric perfusion of physiologic saline, In the Ganoderma lucidum spore-treated group, intraperitoneal injection of PTZ and gastric perfusion of Ganoderma lucidum spore powder were administered. The blank control group was only administered with the physiological saline by intraperitoneal injection and gastric perfusion.MAIN OUTCOME MEASURES: Immunohistochemical staining and radioimmunoassay methods were used to observe the changes of somatostatin and neuropeptide Y content in brain tissue of epileptic rats, as well as the morphology of neurons.RESULTS: All 30 rats were involved in the result analysis, without any loss. The number of somatostatin immunoreacted positive cells in the cerebral cortex and hippocampus was significantly increased in the epilepsy model group compared to the blank control group (P<0.01). The number of somatostatin immunoreacted positive cells in cerebral cortex and hippocampus was significantly decreased in the Ganoderma lucidum spore-treated group compared to the epilepsy model group (P<0.01). The contents of neuropeptide Y in cerebral cortex and hippocampus were significantly increased in the epilepsy model group compared to the blank control group (P<0.01). The contents of neuropeptide Y in the cerebral cortex and hippocampus were significantly decreased in the Ganoderma lucidum spore-treated group compared to the epilepsy model group (P<0.05). The epilepsy seizures in the Ganoderma lucidum spore-treated group were obviously reduced compared to the epilepsy model group.CONCLUSION: Ganoderma lucidum spore powder was able to reduce the somatostatin and neuropeptide Y content in the cerebral cortex and hippocampus effectively, so as to achieve an anti-epileptic function and protect neurons from being damaged.     

Influence of Ganoderma lucidum spores on the levels of neuropeptide Y and somatostatin in brains of seizure rats*★

BACKGROUND： Previous research has revealed that somatostatin can induce epilepsy, and that the levels of neuropeptide Y may increase and become more active in brain areas with epileptic seizures. OBJECTIVE： To observe the effect of Ganoderma luciclum spore powder on the neuropeptide Y and somatostatin content in the cerebral cortex and hippocampal regions of seizure rats induced by pentylenetetrazol （PTZ）. Furthermore, to verify any effect of Ganoderma lucidum spore powder on inhibition of epileptic seizures. DESIGN, TIME AND SETTING： A randomized group animal study was performed in August 2007 in the School of Basic Medical Sciences, Jiamusi University （Jiamusi, Heilongjiang, China）. MATERIALS： Thirty healthy, male, Wistar rats, aged 12 weeks and weighing 180-220 g, were taken as the experimental animals. PTZ （Sigma Company, United States） was used to induce epilepsy. Ganoderma lucidum spores （Leyss, ex Fr variety） were purchased from Jiamusi City Wild Growing Case of the Ganoderma Lucidum （China）. Rabbit anti-somatostatin antibodies and secondary antibodies were purchased from Wuhan Boster Company （China）. Neuropeptide Y radioimmunoassay kit was purchased from Beijing Furui Biotechnology Company （China）. METHODS： Thirty rats were randomly divided into three groups： a control group, an epilepsy model group and a Ganoderma lucidum spore-treated group. Each group contained 10 animals. Rats in the epilepsy model group were treated with intraperitoneal injections of PTZ and gastric perfusion of physiologic saline. In the Ganoderma lucidum spore-treated group, intraperitoneal injection of PTZ and gastric perfusion of Ganoderma lucidum spore powder were administered. The blank control group was only administered with the physiological saline by intraperitoneal injection and gastric perfusion. MAIN OUTCOME MEASURES： Immunohistochemical staining and radioimmunoassay methods were used to observe the changes of somatostatin and neuropeptide Y content in brain tissue of epileptic r     

野生与人工栽培泰山赤灵芝的微量元素对比分析

对野生与人工栽培泰山赤灵芝进行了微量元素对比分析,结果表明:二者均含24种微量元素,且含锗量均较高。野生者所含锰、镁、铜、铈、锶、钡高于人工栽培者。人工栽培灵芝的锌、铁、磷、硼、铬、锗、则高于野生者。     

Triple cones in the retinae of three anchovy species: Engraulis encrasicolus, Cetengraulis mysticetus and Anchovia macrolepidota (Engraulididae, Teleostei)

Histology and fine structure of three anchovy outer retinae is investigated by light- and electron microscopy and computer aided topographic mapping. They all possess novel linear triple cones with two large lateral components and one small central component. Engraulis encrasicolus has a polycone-dominated retina with two triple-cone sectors, the retinae of Cetengraulis mysticetus and Anchovia macrolepidota are triple-cone dominated. Interspecific differences in the configuration of the complex outer retina structures appear well adapted to the requirements of visual orientation in the respective photic habitats. In addition a survey on the occurrence of triple cones in teleosts is given.