三苯氧胺联合米非司酮对ICAM-1、LIF在早孕绒毛中表达的影响

目的:检测3组早孕流产绒毛组织中的细胞间黏附分子-1(ICAM-1)、白血病抑制因子(LIF)的表达,探讨三苯氧胺联合米非司酮缩短流产出血时间的可能作用机理,为临床预防和治疗药物流产后出血提供理论依据。方法:随机将非意愿妊娠7周内要求药物流产的宫内早孕妇女80例分成2组:三苯氧胺联合米非司酮+米索前列醇组(A组);米非司酮+米索前列醇组(B组)。40例人工流产手术为对照组(C组)。应用West-ern blot及实时定量PCR方法分别检测绒毛组织中ICAM-1、LIF的蛋白及mRNA的表达。结果:A组的平均出血时间较B组短,C组出血时间最短。A组绒毛组织中ICAM-1、LIF蛋白及mRNA的表达量最低,B组绒毛组织中ICAM-1、LIF蛋白及mRNA的表达量稍高,C组绒毛组织中ICAM-1、LIF蛋白及mRNA的表达量最高。结论:三苯氧胺可协同米非司酮加强ICAM-1和LIF的表达下调,推测三苯氧胺联合米非司酮缩短药物流产出血时间可能与ICAM-1和LIF的表达变化有关。     

激光诱发荧光光谱诊断肺癌的初步临床研究

目的　探讨利用激光诱发荧光光谱对肺癌进行临床诊断的可行性。方法　使用 35 5nm的Nd :YAG激发光和荧光探测系统 ,将特制光导纤维通过纤维支气管镜的活检通道送入患者支气管 ,对 2 1例患者的正常支气管壁及可疑部位进行激光诱发荧光光谱测定 ,随后在各检测点咬取组织行病理检查。结果　 2 1例患者共测定 74条曲线 ,经病理证实 ,肺癌 32条 ,正常支气管组织 4 2条。正常支气管组织主峰 (4 6 2 4±7 5 )nm和肺癌主峰 (4 6 0 5± 7 0 )nm位置无显著性差异 (P >0 0 5 ) ;正常支气管组织的荧光强度 (84 35 2±5 0 4 2 8)明显大于肺癌 (336 0 2± 196 0 9) (P <0 0 0 1) ;正常支气管组织在 5 80～ 6 0 0nm波段表现为一小坪 (I580nm/I60 0 nm =1 0 6 4± 0 133) ,而肺癌平滑下降 (I580nm/I60 0nm=1 32 9± 0 0 85 ) (P <0 0 0 1)。以I580nm/I60 0nm比值为判据诊断肺癌的敏感性、特异性、阳性预测值、阴性预测值及总符合率分别为 87 5 %、80 9%、77 8%、89 5 %和 83 8%。结论　用激光诱发荧光光谱对肺癌进行临床诊断是可行的。     

Leukemia inhibitory factor promote trophoblast invasion via urokinase-type plasminogen activator receptor in preeclampsia

Preeclampsia is a pregnancy-related syndrome which can cause perinatal mortality and morbidity. Inadequate invasion by trophoblast cells may lead to poor perfusion of the placenta, even result in preeclampsia. Understanding the molecular mechanisms underlying placentation facilitates the better intervention of preeclampsia. Urokinase-type plasminogen activator receptor (uPAR) is involved in the physiological and pathological processes. Leukemia inhibitory factor (LIF) is an important regulator in the establishment of pregnancy. However, the expression of uPAR in preeclamptic patients and its relationship with LIF remains unclear. In the current study, we found that the level of uPAR was relatively lower in the placentas from preeclamptic patients as compared with normal pregnant women. LIF promoted trophoblast cell outgrowth by upregulating uPAR in an explants culture, and LIF also enhanced migration and invasion potential through uPAR in trophoblast JAR and JEG-3 cell lines, and with increased gelatinolytic activities of matrix metalloproteinase 2 (MMP-2). The effect of LIF and uPAR on trophoblast migration and invasion was mediated by PI3K/AKT signaling pathway. Our data indicates the roles of LIF in promoting trophoblast migration and invasion through uPAR and suggest that abnormal expression of uPAR might be associated with the etiology of preeclampsia.     

Regulation of an oligodendrocyte progenitor cell line by the interleukin-6 family of cytokines

We report pleiotropic actions of the interleukin-6 family of cytokines on a rat cerebral cortical oligodendrocyte cell line, Central Glia-4 (CG-4). This is a bipotential oligodendrocyte type-2 astrocyte (O-2A) progenitor cell line that can be manipulated in vitro to become either a type-2 astrocyte or to follow a linear sequence of events into becoming a mature oligodendrocyte. Using Northern and Western analyses in conjunction with immunocytochemistry we have demonstrated that ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and interleukin-6 (IL-6) cause a transient increase in glial fibrillary acidic protein (GFAP) in oligodendrocyte type-2 astrocyte (O-2A) progenitor cells. At maximal cytokine concentrations, the largest increase in GFAP protein levels were observed for CNTF and LIF; albeit, IL-6 did increase GFAP but the order of magnitude was 6-7 times less. Moreover, in trophic factor deprived medium, CNTF and LIF protected immature (O4⁺/MBP^?) and mature (MBP⁺) oligodendrocytes from the apoptotic mode of cell death, while IL-6 had no effect in enhancing oligodendrocyte cell survival. Analysis of the cytokine-induced early response genes (ERGs) revealed a strong degree of overlap for CNTF and LIF. The effect of IL-6 was different in the degree to which the ERGs were up-regulated and in their temporal patterns of expression. These findings suggest that ERGs may be important, at least in part, for determining the extent of functional overlap observed within this cytokine family. Our findings clearly demonstrate differential regulation of oligodendrocyte survival and differentiation by the IL-6 family of cytokines.     

白血病抑制因子在原发不孕症患者子宫内膜的表达

目的 :探讨白血病抑制因子 ( LIF)在子宫内膜的表达与原发不孕症的关系。方法 :采用逆转录聚合酶链反应 ( RT- PCR)技术 ,检测 30例原发不孕症患者 (观察组 )和 2 0例对照组患者分泌中晚期子宫内膜 LIF m RNA的表达。结果 :对照组患者分泌中晚期子宫内膜 LIF m RNA的表达水平为 1 .872± 0 .431 ,观察组为 0 .42 6± 0 .1 0 8,两组相比 ,差异有显著性 ( P<0 .0 0 1 )。结论 :LIF表达的减少或缺失 ,可能是原发不孕症的原因之一     

Development of a multivariate light-induced fluorescence (LIF) PAT tool for in-line quantitative analysis of pharmaceutical granules in a V-blender

Process analytical technologies (PAT) enable process insight, process control and real-time testing. Light-induced fluorescence (LIF) spectroscopy is especially well suited for low-concentration ingredients as, in many cases, it is the most sensitive probe of the in-line PAT toolbox. This study is aimed at verifying the applicability of a multivariate LIF analyzer to monitor granulated powder blends in industrial settings. Its targets are to: (1) evaluate the critical parameters of powders to obtain robust, precise and accurate concentration predictions and (2) assess technology performance for in-line monitoring of blending operations. Varying dye properties, moisture levels and particle sizes have been shown to have the most significant impact on fluorescence emission. Reliable quantitative models can be obtained by controlling and/or mitigating these factors.     

Tissue Reaction and Metal Sensitivity: An Animal Study

An animal study is presented in which nickel sensitivity as determined by an in vitro test for leukocyte migration inhibition (LIF) is correlated with results of skin tests with NiCl₂ and with the degree of adverse tissue reaction to implanted stainless steel screws. Screws were implanted in the humeri of New Zealand white rabbits, one group of which received repeated injections of nickel chloride following an initial injection of nickel chloride in Freun d?s adjuvant. All the injected rabbits became skin test positive to nickel and demonstrated LIF production in the presence of nickel chloride. Some of the injected rabbits, 6–9 weeks after implantation of the screws, developed an inability for leukocyte migration even when the cells were incubated only with saline and serum. the tissue reaction to screws in the nickel sensitive animals showed a significant increase in inflammatory cells and foreign body giant cells when compared with the reaction in nonsensitive animals. the most severe reactions, some of which included regions of necrosis, were seen in animals which developed the condition of no leukocyte migration in the LIF test. the correlation between skin test and LIF test results demonstrates the effectiveness of the LIF assay for sensitivity testing without the risk of sensitization associated with skin testing. the histological results support the hypothesis that the condition of no cell migration in the LIF test is diagnostic for severe reactions to implants associated with metal sensitivity.     

干细胞因子和白血病抑制因子对冻融后人原始卵泡体外生长发育的研究

目的:探讨干细胞因子(stem cell factor,SCF,又称KIT配体)和白血病抑制因子(leukaemiainhibitory factor,LIF)对冻融后人原始卵泡体外培养生长发育的影响。方法:收集18例卵巢良性肿瘤手术患者的正常卵巢皮质,采用直接覆盖玻璃化冷冻法保存卵巢组织,冻融后分离卵泡,随机分成5组:对照组(A组)、10 ng/ml SCF组(B组)、10 ng/ml LIF组(C组)、10 ng/ml SCF+10 ng/ml LIF组(D组)和100 ng/ml SCF+10 ng/ml LIF组(E组),行体外培养,比较SCF和/或LIF对人原始卵泡生长发育的影响。结果:①随培养天数的增加,对照组卵泡直径增长缓慢,明显小于各实验组(P<0.05);D、E组中卵泡直径增长迅速,较B、C组差异有统计学意义(P<0.05)。②随着培养天数的增加,各组卵泡均能分泌激素。4 d后对照组卵泡激素水平增长缓慢,明显低于各实验组(P<0.05),而D、E组中卵泡激素分泌增长迅速,较B、C组差异有统计学意义(P<0.05),D、E组中卵泡激素增长速度相差不大,但在第4日差异有统计学意义(P<0.05)。结论:在体外培养液中添加SCF或LIF以及两者联合添加有利于促进卵泡的生长发育,提高E2分泌功能,且SCF和LIF联合其作用更加明显。     

935MHz微波电磁辐射对小鼠着床期内膜细胞LIF蛋白表达的影响

目的:研究935MHz微波电磁辐射对小鼠着床期内膜细胞LIF蛋白表达的影响。方法:将受精后小鼠暴露于不同时间、不同强度的935MHz辐射波连续辐射3天,于受孕第4天取孕鼠子宫内膜,运用Western blot方法检测细胞内LIF蛋白的表达。结果:在辐射时间相同的情况下,高强度4h/d组和中强度4h/d组子宫内膜腺上皮细胞内膜LIF表达量明显下降;辐射强度相同时,中强度4h/d组与中强度2h/d组组比较、高强度4h/d组组与高强度2h/d组比较,LIF表达量也有明显减弱,显示辐射时间的长短可能影响LIF的表达,且微波对LIF表达的抑制作用具有蓄积效应。结论:935MHz微波电磁辐射通过影响孕鼠胚胎着床期子宫内膜细胞内LIF蛋白的表达,导致子宫内膜容受性发生改变,进而影响胚胎着床过程。