消脂饮对大鼠实验性高脂血症防治作用的研究

目的：探讨消脂饮对实验性高脂血症大鼠的影响。方法：采用饲喂高脂乳剂建立高脂血症模型,测定脂类代谢中生化指标,及血清、心肌中超歧过氧化物酶(SOD)活性和过氧化反应产物丙二醛(MDA)含量。结果：消脂饮各组与模型组比较均能明显的降低高脂血症大鼠血清中总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白(LDL-C)含量,降低血清和心肌中MDA含量,提高血清和心肌中SOD活性。结论：消脂饮对实验性高脂血症大鼠具有预防和治疗作用,其机理可能是通过清除自由基,提高机体抗氧化能力。     

Anti-oxidation activity of the crude polysaccharides isolated from Polygonum Cillinerve (Nakai) Ohwi in immunosuppressed mice

Aim of the study

Polygonum Cillinerve (Nakai) Ohwi is commonly used in China for over 2000 years. Previous research has shown that the crude polysaccharides extracted from Polygonum Cillinerve (Nakai) Ohwi (PCCP) have the scavenging free radicals and anti-tumor activities in vitro. In present study, PCCP were further approached the perspective of their anti-oxidation in immunosuppressed mice.

Methods and materials

ICR mice were treated firstly with cyclophosphamide (CY, 150 mg/kg), 1 day later, treated with different dosages of PCCP or saline solution once daily for 21 days. Twenty-four hours later for the last drug administration, the animals were weighed, and then killed by decapitation. The liver, spleen, and thymus indices were investigated, and the biochemical parameters were evaluated for various tissues (liver, heart, and kidney).

Results

The administration of PCCP with gavage was able to overcome the cyclophosphamide-induced immunosuppression, and significantly to raise the TOC, CAT, SOD, and GSH-Px level. It also raised the liver, spleen, and thymus indices, and decreased the MDA level in mice.

Conclusions

PCCP possess the pronounced free radical-scavenging and antioxidant activities, and could play an important role in the prevention of oxidative damage in immunological system.     

PBNA-413对离体心脏缺血再灌注损伤时抗氧化作用的影响

目的 研究拳参正丁醇提取物(PBNA)的水溶性成分PBNA-413对离体心脏缺血再灌注损伤时的抗氧化作用.方法 应用大鼠离体心肌缺血再灌注损伤模型,在灌流液中加入高、中、低剂量的拳参正丁醇提取液进行再灌注.再灌注结束后,测定心肌组织乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)的含量.结果 各剂量的PBNA-413治疗后,均能提高心肌组织LDH(P<0.01)、SOD(P<0.05)、GSH-Px(P<0.01)的活性,升高NO(P<0.05)含量,降低MDA(P<0.001)及LD(P<0.01)的含量,且呈明显的剂量依赖性.结论 拳参正丁醇提取物可通过提高抗氧化酶系统的功能、减少心肌酶的释放而保护缺血再灌注心肌.     

螺旋藻多糖对糖尿病小鼠抗氧化能力的影响

腹腔注射四氧嘧啶（ＡＬＸ）２００mg/kg建立糖尿病模型小鼠,并随机分为４组,分别用螺旋藻多糖（ＰＳＰ）１００mg/kg、２００mg/kg及阳性对照药优降糖２mg/kg灌胃给药,糖尿病模型对照组则给等容积生理盐水,连续１４d后,分别测定各组小鼠血糖、血清ＳＯＤ活性、ＭＤＡ含量及全血ＧＳＨ－Ｐx活性与ＧＳＨ含量。结果与糖尿病模型对照组相比,ＰＳＰ能使ＡＬＸ所致糖尿病小鼠血糖降低（Ｐ＜０.０１）;血清ＳＯＤ活性、全血ＧＳＨ－Ｐx活性及ＧＳＨ一显著回升（Ｐ＜０.０１）;血清ＭＤＡ含量明显下降（Ｐ＜０.０１）。结果表明ＰＳＰ能降低ＡＬＸ性糖尿病小鼠高血糖并显著增强其抗氧化能力。提示ＰＳＰ降血糖作用的机制可能与其增强糖尿病鼠抗氧化能力有关。     

抗艾滋病中成药唐草片抗氧化性的研究

目的：研究唐草片对羟自由基的清除作用。方法：采用分光光度法考察唐草片对Fenton反应产生的羟基自由基的清除作用,并采用维生素C作为阳性对照。利用大孔树脂对其中的抗氧化成分进行初步分离。结果：唐草片具有较好的羟自由基清除作用,且呈剂量依赖性,发现其中所含的总黄酮类成分作用较强。结论：唐草片具有一定的抗氧化性。     

丙戊酸镁对小鼠急性脑缺血再灌注损伤的预防作用研究

目的:探讨丙戊酸镁对小鼠急性脑缺血再灌注损伤的预防作用及其机制。方法:取小鼠随机分为假手术组、模型组和丙戊酸镁高、低剂量(0.04、0.02mg·g-1)组,每组10只,灌胃给予相应药物,每日2次,连续14d,末次给药1h后对后3组小鼠建立急性脑缺血再灌注损伤模型,建模成功后检测各组小鼠脑指数和脑组织中丙二醛(MDA)含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性,并观察大脑皮层和海马CA1区细胞形态的变化。结果:与模型组比较,丙戊酸镁高、低剂量组小鼠神经元损伤减轻,脑指数和MDA含量明显降低,GSH-Px活性明显升高(P<0.01或P<0.05),且丙戊酸镁高剂量组SOD活性明显升高(P<0.01)。结论:丙戊酸镁对小鼠急性脑缺血再灌注损伤具有预防作用,其机制可能与抗氧化作用有关。     

虎杖提取物对小鼠急性肝损伤的保护作用

目的观察虎杖提取物对cch诱导的小鼠急性肝损伤的保护作用。方法采用CCl4诱导小鼠急性肝损伤模型,测定血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AsT）、肝组织超氧化物歧化酶（S（）D）活性及丙二醛（MDA）含量。结果cch诱导的小鼠急性肝损伤摸型,血清ALT、AST明显升高,肝组织SOD活性明显降低,MDA含量显著升高（P〈0．01）;虎杖提取物能显著降低血清ALT,AST,明显提高肝组织SOD活性,降低肝组织MDA含量（P〈0．01）。结论虎杖提取物具有降酶及抗氧化的作用。对CCh诱导的小鼠急性肝损伤具有一定的保护作用。     

六味地黄醇提水洗脱部位对D-半乳糖所致小鼠衰老模型的影响

目的:探讨六味地黄醇提水洗脱部位对D-半乳糖所致小鼠衰老模型的影响,评价其延缓脑衰老的作用.方法:ICR小鼠随机分为5组:空白组、模型组、维生素E组、六味地黄醇提水洗脱部位低、高剂量组.皮下注射D-半乳糖0.14 g·kg-1·d-1制备小鼠亚急性衰老模型.用跳台和Morris水迷宫法检测各组小鼠的学习记忆能力.用酶标法测定各组小鼠血清和脑组织匀浆中衰老相关指标.结果:六味地黄醇提水洗脱部位能使衰老小鼠跳台实验反应时间缩短,错误次数显著减少;水迷宫试验逃避潜伏期时间缩短,穿越次数增多(P＜0.05或P＜0.01),提高衰老模型小鼠血清SOD、GSH-Px及脑组织Na++-K+-ATP酶活性,并升高GSH/GSSG比值,减少血清MDA和脑组织NO含量,降低NOS活性(P＜0.05或P＜0.01);减少脑内Aβ含量,但与模型组比较无显著性差异.结论:六味地黄醇提水洗脱部位能够改善D-半乳糖衰老模型小鼠的学习记忆障碍,提高脑组织的抗氧化能力,具有一定延缓脑衰老的作用.     

人参皂苷Rg1对豚鼠急性心肌缺血的保护作用

目的 观察人参皂苷Rg1对豚鼠急性心肌缺血的保护作用。方法 建立垂体后叶素致豚鼠急性心肌缺血模型,观察人参皂苷Rg1对垂体后叶素诱发心肌缺血豚鼠心电图T波和ST段的影响,测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、肌酸磷酸激酶(CK)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性和丙二醛(MDA)含量的变化。结果 人参皂苷Rg1能抵抗对垂体后叶素诱发豚鼠心电图T波和ST段的抬高(P＜0.05,P＜0.01),降低血清中AST、CK和LDH活性(P＜0.05,P＜0.01),升高血清中SOD和GSH-Px活性和明显降低MDA含量(P＜0.05,P＜0.01)。结论 人参皂苷Rg1对垂体后叶素致豚鼠急性心肌缺血具有明显的保护作用。     

Neuroprotective effects of the Chinese Yi-Qi-Bu-Shen recipe extract on injury of rat hippocampal neurons induced by hypoxia/reoxygenation

Objective

To explore the protective effects of the Chinese Yi-Qi-Bu-Shen recipe (YB) against neuronal injury induced by hypoxia–reoxygenation, which has shown beneficial effect in improving the brain function of type 2 diabetics likely through its antihyperglycemic, antioxidant activity, and investigate its mechanisms.

Methods

The bilateral hippocampus was collected from newborn rats to establish single cell suspension. On the 10th day, the primarily cultured hippocampal neurons were randomly divided into five groups: the normal group (NG), the hypoxia/reoxygenation group (HG), and groups protected with small, medium and large dosages of YB (SG, MG and LG, respectively). The YB-protected groups were treated with different concentrations of YB containing serum before reoxygenation. The metabolic rate of MTT, the malondialdehyde (MDA) content, and the activity of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) were measured with assay kits. The apoptosis rate of hippocampal neurons were tested using flow cytometry analysis. RT-PCR was used to evaluate the mRNA expressions of bcl-2 and bax genes.

Results

The SOD activity, the cell survival rate, the bcl-2/bax ratio, and the bcl-2 mRNA expression in the HG group were significantly lower (all P<0.01), but the levels of MDA and LDH, the apoptosis rate, and the bax mRNA expression were higher (all P<0.01) than those in the NG group. The SOD activity, the cell survival, the bcl-2 mRNA expression, and the bcl-2/bax ratio were significantly higher in all of the YB-protected groups (all P<0.01), but the level of MDA and LDH, the apoptosis rate, and the bax mRNA expression were lower (P<0.01, P<0.05) than those in the HG group in a dose dependent manner.

Conclusion

The YB extract has a protective effect on hippocampal neurons against injury induced by hypoxia/reoxygenation, through its antioxidant activity and the regulation of apoptosis.