《中药药剂学》教学情况的调查分析

采用问卷调查形式,对湖南中医药大学接受了中药药剂学教学的2005级、2006级中药学专业本科生进行教学情况的调查。通过对调查结果的分析,总结了中药药剂学教学中取得的成效,提出了今后教学过程中还需要改进和完善的地方。     

药剂学实验教学改革与创新

药剂学是药学专业的一门必修课,实验教学是整个教学过程中一个非常重要的环节。本文针对药剂学实验教学的现状,提出实验教学改革的原则、目标和基本措施,通过构建以学生为主的教学模式、改革实验内容、完善考核制度等方式,最大限度地调动学生的主观能动性,培养学生的实验设计和创新能力,提高学生的专业综合素质。     

HPLC测定不同工艺柴苓汤颗粒剂中柴胡皂苷a的含量

目的 测定和比较日本和国产不同工艺制柴苓汤颗粒剂中指标成分柴胡皂苷a的含量。方法采用HPLC,色谱柱为Kromasil C18（250mm×4．6mm,5μm）;流动相为甲醇-水（75：25）;流速0．8mL·min^-1;柱温30℃;检测波长为210nm。结果柴胡皂苷a线性范围为4．0～15．0μg,r=0．9999,加样回收率为100．39％,RSD为0．80％。结论柴胡皂苷a的含量为日本津村柴苓汤颗粒剂高于国产仿日本工艺制柴苓汤颗粒剂高于国产习用工艺制柴苓汤颗粒剂。本方法简捷、方便、重复性好,准确度高,可用于柴苓汤颗粒剂的质量检测。     

分析化学教学中对药学学生科研能力的培养探析

探索分析化学教学中培养药学学生科研能力的教学改革思路,通过强调分析化学在药学科研中的重要性,改进传统教学方法,结合综合性实验教学和开展课外科研活动等手段,使药学学生接受科研能力的基本训练,收到了较好的教学效果。     

药学生物理化学教学内容改革初探

物理化学是药学生的一门重要的专业基础课,在物理化学教学中强化药学专业特色至关重要,我们通过对理论和实验教学内容进行教学改革,取得了较好效果。     

A Rapid Spectrofluorimetric Technique for Determining Drug-Serum Protein Binding Suitable for High-Throughput Screening

Purpose. To develop and validate a rapid method for determining thedissociation constants with which pharmaceutical candidates and drugsbind to serum albumin and to ₁-acid glycoprotein with the goal ofdeducing the extent of binding. Methods. The quenching of the intrinsic tryptophan fluorescence ofserum albumin and ₁-acid glycoprotein was monitored byspectrofluorimetry and the data were used to calculate the apparent dissociationconstant. Sodium warfarin was used to probe the warfarin-binding siteof serum albumin and diazepam was used to probe the benzodiazepinebinding site. Additionally, the binding of sodium salicylate, phenylbutazone, sulfinpyrazone, iophenoxic acid, theophylline, chloramphenicol,acetaminophen, lithium chloride and ampicillin were also investigated.Chlorpromazine hydrochloride and imipramine hydrochloride wereused as probes for ₁-acid glycoprotein. The assays were also extendedto the multiwell format. The quenching curves were fitted to thequadratic binding equation to determine the dissociation constants. Results. Intrinsic fluorescence measurements are an excellent predictorof the drug binding to human serum albumin and to ₁-acidglycoprotein. These measurements detect binding to the warfarin andbenzodiazepine binding sites of human serum albumin. The dissociation constantsestimated using the method compare favorably to the dissociationconstants previously reported by Epps et al. using extrinsic fluorescencemethodology, and the results correlate well with equilibrium dialysisusing drug displacement endpoints. Conclusions. These measurements can be carried out with smallsamples and do not require separation of the bound and unbound species.Additionally, the proposed methods eliminate membrane separations,are not compound specific and do not require analyticalchromatography or mass spectrometry for quantitation. Spectrofluorimetry mayprove to be a useful method for rapidly determining the protein bindingof combinatorial libraries.     

补肾壮骨颗粒的最佳成型处方筛选

以不同辅料与补肾壮骨方提取的稠膏按不同比例配伍制成药粉及颗粒后，以颗粒剂的制剂学参数为指标，筛出了补肾壮骨颗粒的最佳成型处方组成。并为成型及分装车间控制相对湿度提供了理论依据。     

医疗机构制剂的现状与未来发展

本文回顾了医疗机构制剂的发展历史,分析讨论了有关法律法规相继实施后,医疗机构制剂的现状和存在的困惑。预测了医疗机构制剂的未来发展。     

药物缓释、控释制剂的研究开发现状及发展趋势

近年来国内外研制成缓释、控释制剂的主要有片剂、胶囊剂、微球剂、滴眼剂、注射植入剂等。目前缓释、控释制剂技术发展迅速,其研制开发有广阔的市场和临床前景。     

新型凝胶剂基质聚丙烯酰胺的初步研究

 目的　研究聚丙烯酰胺(PAM)作为凝胶剂基质的药剂学性质。方法　用NDJ-1型旋转黏度计测定其在不同浓度下的黏度,并考察温度、pH值、盐类及乙醇对其黏度的影响;用家兔作为实验动物,考察PAM对眼及皮肤的刺激性。结果　PAM对酸碱及乙醇有一定的耐受性,PAM能耐受低温贮存和高压湿热灭菌,但不能耐受盐类;PAM有良好的生物相容性,对眼及皮肤无刺激。结论　PAM凝胶制备工艺简单,质量易控,有良好的药剂学性质和生物相容性,是一种有广阔开发前景的凝胶剂基质。