Effects of basic fibroblast growth factor on superoxide dismutase activity and malondialdehyde content in the rat brain following intracerebral hemorrhage

BACKGROUND: Studies have confirmed that basic fibroblast growth factor (bFGF) promotes neuronal survival and neurite outgrowth. OBJECTIVE: To compare and verify the effects of bFGF on superoxide dismutase activity and malondialdehyde content in rat brain tissues surrounding a hemorrhagic lesion, as well as the hippocampus at the hemorrhagic side. DESIGN, TIME AND SETTING: The randomized, controlled, neurobiological study was performed at the Science Experimental Center and Research Laboratory, Guangxi Medical University, China, from September to December 2006. MATERIALS: Ninety-two adult, healthy, Wistar rats of equal gender were used to establish intraeerebral hemorrhage by infusing type VII collagenase into the left internal capsule. Type Ⅶ collagenase (Sigma, USA), superoxide dismutase and malondialdehyde kits (Jiancheng, China), and bFGF (Institute of Bioengineering, Ji'nan University, China) were used for this study. METHODS: Ninety successfully lesioned rats were equally and randomly divided into three groups. Rats in the bFGF group were intramuscularly injected daily with bFGF (8μg/kg). Rats in the saline control group received an equal volume of saline. The rats in the model group did not receive other interventions. Superoxide dismutase activity was measured using the xanthine oxidase method. Malondialdehyde contents were detected using the thiobarbituric acid method. MAIN OUTCOME MEASURES: At 1, 3, and 7 days following intracerebral hemorrhage, superoxide dismutase and malondialdehyde were determined in the brain tissue surrounding the hematoma and in the hippocampus in the affected hemisphere. RESULTS: In brain tissue surrounding the hematoma, superoxide dismutase activity was significantly increased in the bFGF group at 3 and 7 days after intracerebral hemorrhage compared with the saline control group, whereas malondialdehyde content was significantly decreased (P < 0.05). In the hippocampus, superoxide dismutase activity was significantly increased in the bFGF group at 7 days following intracerebral hemorrhage compared with the saline control group, whereas malondialdehyde content was significantly decreased (P < 0.05). At 1, 3, and 7 days after intracerebral hemorrhage, there was no significant difference between the saline control group and the model group with regards to parameter or brain region (P > 0.05). CONCLUSION: Increased superoxide dismutase activity and decreased malondialdehyde content were detected in tissue surrounding the hematoma, as well as the ipsilateral hippocampus, of intracerebral hemorrhage rats treated with bFGF. Changes in these parameters were detected earlier in tissue adjacent to the lesion, compared with the ipsilateral hippocampus.     

蒲金口服液对缺氧缺血性脑损伤大鼠自由基的影响研究

目的 :通过蒲金口服液对缺氧缺血新生大鼠脑组织自由基的影响 ,探讨急性缺氧缺血性脑病的发病机制和化痰活瘀治疗的作用机理。方法 :采用 7日龄SD大鼠结扎右侧颈总动脉和置入低氧环境中诱发的缺氧缺血性脑损伤模型 ,于造模后即刻、2h、4h、6h、8h、1 0h ,将 1日药量共分为 6次灌胃 ,造模后 2 4h处死大鼠 ,取右侧顶枕区脑组织制成 1 0 %脑组织匀浆 ,用可见光分光光度计通过比色法测定超氧化物歧化酶 (SOD)、丙二醛 (MDA)含量。结果 :蒲金口服液高剂量组与缺氧缺血性脑损伤模型组相比 ,有极显著差异 (P <0 .0 1 ) ;蒲金口服液高剂量组与达纳康组相比 ,差异有显著性 (P <0 .0 5 )。结论 :蒲金口服液高剂量组能显著升高缺氧缺血性脑损伤大鼠脑组织中SOD水平 ,降低MDA含量 ,具有明确的抗自由基作用     

镁对缺血后再灌注心肌脂质过氧化物的影响

利用Ｌａｎｇｅｎｄｏｒｆｆ装置，制成大鼠心肌缺血－再灌注模型，观察镁对缺血后再灌注心肌脂质过氧化物（ＭＤＡ）的影响。结果表明灌注液中缺失镁，可增加再灌注心肌ＭＤＡ值（Ｐ〈０．０５），而高镁可降低再灌注心肌ＭＤＡ值（Ｐ〈０．０５）。提示镁能减少心肌缺血后再灌注时自由基的产生，从而对心肌缺血后再灌注损伤有一定保护作用。     

黄芩苷对大鼠心肌缺血再灌注损伤的保护作用

目的　研究黄芩苷对大鼠缺血再灌注损伤的保护作用。方法　结扎大鼠左冠脉前降支 4 0min再灌注 12 0min ,观察黄芩苷对心肌梗死后大鼠心功能、心肌梗死面积和乳酸脱氢酶 (LDH)活性、丙二醛 (MDA)含量、超氧化物歧化酶(SOD)活性等的影响。结果　结扎冠脉前 5min给黄芩苷10～ 4 0mg·kg-1能改善心肌梗死后大鼠的心功能、减少心肌梗死面积 (9%～ 30 % ) ,并能减少梗死后心肌MDA含量、提高梗死后心肌SOD、LDH的活性。结论　黄芩苷对大鼠缺血再灌注的心肌损伤有保护作用     

深低温停循环血液稀释时大鼠海马MDA、LDH和ATP酶的变化

目的观察不同程度血液稀释对深低温停循环(DHCA)大鼠海马丙二醛(MDA)、乳酸脱氢酶(LDH)和ATP酶的影响。方法建立大鼠DHCA(18℃，90min)模型，随机分成四组：Hct10％组、Hct20％组、Hct30％组和假手术对照组。模型成功后取海马，应用比色法检测MDA、LDH和ATP酶的变化。结果Hct30％组与Hct20％组和Hct10％组相比，海马MDA含量最低，但仍然高于假手术组；LDH和ATP酶的活性最高，但仍低于假手术组。结论在DHCA期间保持较高的Hct值(30％)具有脑保护作用。     

空降兵跳伞后血浆的生化变化:初步调查与分析

目的 探讨跳伞期间空降兵经历的生理和心理应激是否会改变空降兵血浆的生化指标。方法 调查了对照组（末跳伴组）和跳伴组空降兵血浆和血清中激素水平（皮质醇，生长激素，胰岛素，胰高血糖类、类皮素，血管紧素Ｉ和Ⅱ、醛固酮）、酶活性（超氧化物歧化酶、谷胱甘肽过氧化氢酶和谷胱甘肽硫转移酶）、自由基损伤指标水平（丙二醛）、肿瘤坏死因子α（ＴＮＦ－α）和主要热应激蛋白（ＨＳＰ７０）的差异性。结果 跳伴组血清中生长激     

异丙酚对大鼠肾缺血再灌注损伤肾组织病理变化的影响

目的:探讨异丙酚对大鼠肾缺血再灌注损伤的影响。方法:雄性SD大鼠50只,随机分为对照组(C),肾缺血再灌注组(Ⅰ),异丙酚预先给药组(P1)、即时给药组(P2)及延迟给药组(P3)。P1、P2、P3组分别于阻断前1h、阻断同时及再灌注同时缓注异丙酚30mg/(kg.h)3h、4h、4.75h,24h处死大鼠。取肾组织作HE染色及测定丙二醛(MDA),超氧化物歧化酶(SOD)和Ca2 的变化。结果:P1和P2组与I组相比明显改善肾组织损伤,P3组无变化。P1和P2组与Ⅰ组比较MDA和Ca2 水平显著降低,SOD活性增强(P<0.01或0.05),P3组无此作用。结论:异丙酚预先和即时给药明显改善肾损伤,延迟给药无作用。     

健脑灵对小鼠常压耐缺氧能力和组织中丙二醛含量的影响

[目的]探讨健脑灵对小鼠常压耐缺氧能力和组织中丙二醛含量的影响.[方法]采用常压耐缺氧的方法及硫代巴比妥酸法测定了健脑灵组及对照组小鼠的存活时间及心脏及肝脏组织中丙二醛的含量.[结果]健脑灵能明显延长小鼠常压耐缺氧存活时间,降低组织中丙二醛的含量.[结论]健脑灵具有增强耐缺氧能力及降低组织中丙二醛含量的作用.     

中药酒速愈对急性酒精中毒小鼠肝脏氧化酶的影响

目的:观察中药酒速愈对急性酒精中毒小鼠肝组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性及丙二醛(M DA)含量的影响。方法:采用乙醇体积分数为56%的红星二锅头灌胃建立急性酒精中毒小鼠模型。80只健康昆明种小鼠按随机数字表法分为正常对照组、模型组、酒速愈高剂量组、酒速愈低剂量组和葛花解酲汤组,每组16只。各组于给药后6 h分别检测小鼠肝组织SOD、CAT活性和M DA含量。结果:与正常对照组比较,模型组小鼠肝组织M DA含量明显升高(P<0.01),SOD和CAT活性均明显降低(P均<0.01);与模型组比较,各治疗组均可升高小鼠肝组织SOD和CAT活性(P<0.05或P<0.01),并可降低M DA含量(P<0.05或P<0.01);与葛花解酲汤组比较,酒速愈高、低剂量组小鼠肝组织CAT活性均明显升高(P均<0.05)。结论:酒速愈能显著升高模型小鼠肝组织SOD和CAT活性,降低M DA含量,抑制脂质过氧化反应,具有抗氧化作用,从而通过调节和改善自由基代谢,减轻急性酒精中毒对肝脏的氧化损伤。