吡哌酸片的电荷转移反应分光光度测定

研究了吡哌酸与四氯苯醌间的荷移反应．两者在硼砂缓冲溶液中形成１∶１的络合物，其λ_（ｍａｘ）为３３０．８ｎｍ，摩尔吸光系数ε＝１．６６×１０~４，ＲＳＤ为２．２％。用于测定制剂含量，结果与药典方法一致。     

流动注射分析法测定醋酸泼尼松片含量均匀度的研究

根据蓝四氮唑与泼尼松生成蓝色产物,并于525nm 波长处测定吸光度。测定条件为:反应管长度为3.8m(0.5mm id);流速为1.6ml/min;反应温度为55±0.5℃;注射样品溶液为100μl;本测定系统的测定速度可达100次/h;线性范围可达0.09mg/ml;检测限量为1.54μg/ml;测定结果的变异系数小于1%。     

离子对提取比色法测定复方制剂中氨基比林的含量

复方制剂中氨基比林与溴甲酚绿形成稳定的离于对，用氯仿萃取后在４１６．８ｎｍ处测定吸收度．回收率为９９．８０％，相对标准偏差为０．９２％（ｎ＝５）。方法简便快速，结果准确可靠。     

大鼠长期服苯妥英锌或苯妥英钠对体内锌铜代谢的影响

本文用原子吸收分光光度法测定了大鼠分别服用苯妥英锌和苯妥英钠13周后(每天剂量为12mg/kg体重),肝脏、全脑、齿龈和血清锌铜的含量。苯妥英锌使齿龈和肝脏锌分别增高80％和49％,全脑和血清锌分别降低39％和35％,而苯妥英钠使齿龈和肝脏锌分别降低70％和28％,血清锌增高41％。两药均使全脑和齿龈铜降低。     

Accumulation of toxic metals (Pb and Cd) in the sea urchin Diadema aff. antillarum Philippi, 1845, in an oceanic island (Tenerife,Canary Islands)

This document shows the results obtained from a study on the concentration of toxic heavy metals in the internal tissue and exoskeleton of sea urchins, collected from their natural habitat. The levels of lead and cadmium were measured by Graphite Furnace Atomic Absorption Spectrometry. The mean concentrations of lead and cadmium in the internal tissue were 304.04 and 260.54 μg/kg respectively, whereas in the shell they were 185.02 and 142.48 μg/kg. We also performed a statistical analysis of the differences in the distribution of metals between their exoskeleton and their internal content, a correlation study of the metal content in internal tissue and shell and sampling areas, and a correlation study between the metal content and sample size. Since the sea urchin Diadema antillarum presents a wide range of variation in metal content, this study suggests that this species is an excellent bioindicator of heavy metal contamination. © 2009 Wiley Periodicals, Inc. Environ Toxicol, 2010.     

茶叶中微量锰的新催化光度法测定

利用于氨三乙酸存在下，锰（Ⅱ）对高碘酸钾氧化二甲苯蓝ＦＦ的催化效应，建立了一个新的测定微量锰的动力学分光光度法。该法检出限为６×１０￣（－２）ｎｇ·ｍｌ￣（－１），线性范围为０．２～８．０ｎｇ·２．５ｍｌ￣（－１），常见离子基本不干扰测定。用于直接测定健齿茶和茉莉花茶中的锰含量，结果发现，锰含量分别为２２１４μｇ·ｇ￣（－１）及６４８μｇ·ｇ￣（－１）。此法简便易行，灵敏度高。     

The accuracy of a fiberoptic oximeter over a wide range of arterial oxygen saturation values in piglets

A piglet model was used to evaluate the accuracy of a fiberoptic oximeter over a wide range of arterial oxygen saturation (SaO₂) values. In eight anaesthetized piglets, the inspired oxygen concentration was varied from 30% to 6% resulting in a SaO₂ range from 100% to 15%. Paired data of the Opticath® fiberoptic catheter, which was placed in the descending aorta, and blood sample SaO₂ values assessed by a multiwavelength oximeter, were analysed. After in vitro calibration according to the manufacturer's instruction, the fiberoptic catheter started to underestimate the SaO₂ below 78%, worsening towards lower SaO₂ values. The overall bias was -3. 4% and the precision 3. 8%. An off-line fit with a non-linear model resulted in a standard deviation of residuals of 2. 6%. After several in vivo calibration adjustments when the Fiberoptic oximeter deviated more than 4% from the blood sample value, the bias was eliminated over the total SaO₂ range and the precision was 3. 7%. The Opticath® fiberoptic oximeter could have an accuracy for the whole SaO₂ range between 15–100% close to the accuracy of the multiwavelength oximeter, when the fiberoptic oximeter is adapted for the underestimation below 78% SaO₂.     

Drug-Biomacromolecule Interaction XII: Comparative binding study of sulfaethidole to bovine serum albumin by equilibrium dialysis,fluorescence probe technique,uv difference spectrophotometry and circular dichroism

Binding of sulfaethidole to bovine serum albumin was studied by equilibrium dialysis, fluorescence probe technique, uv difference spectrophotometry and circular dichroism. Equilibrium dialysis method enabled us to estimate the total number of drug binding sites of albumin molecule. For sulfaethidole, albumin had 6 primary and 40 secondary binding sites. The primary and secondary binding constants were 0.9×10⁵ M⁻¹ and 0.2×10⁶ M⁻¹, respectively. 1-Anilino-8-naphthalenesulfonate (ANS) and 2-(4′-hydroxylbenzeneazo)-benzoic acid (HBAB) were used as the fluorescence probe and the uv spectrophotometric probe, respectively. In fluorescence probe technique, results indicated that the number of higher affinity drug binding site of albumin was 1 and the number of lower affinity drug binding sites of albumin was 3, and the primary and secondary drug binding constants for bovine serum albumin were 2.15×10⁵ M⁻¹ and 1.04×10⁵ M⁻¹, respectively. In uv difference spectrophotometry, binding sites were 3 and binding constant was 1.88×10⁵ M⁻¹. The above results suggest that several different methods should be used in ompensation for insufficient information about drug binding to albumin molecule given by only one method.     

茶多酚口含片中总儿茶素的含量测定

在酸性介质中对香英兰素与儿茶素的显色反应进行了研究，改进了反应条件，以５０６ｎｍ为测定波长，测定ＴＰ口含片中儿茶素的含量，线性范围７．５８μｇ～５３．０５μｇ（ｒ＝０．９９９８），回收率９７．９７％，ＲＳＤ１．８５％，认为改良法简便，灵敏，口含片辅料无干扰，可用于原料的药和制剂的含量分析。