Effect of stimulation of Kupffer cells by prodigiosan on regeneration of hepatocytes

The effect of the polysaccharide ofBacillus prodigiosus (prodigiosan) on DNA synthesis and mitotic activity of the hepatocytes was studied in the regenerating liver of male Wistar rats. After administration of the stimulator in a dose of 50 g per rat 24 h before partial hepatectomy the indices of labeled nuclei and mitotic indices were higher and reached their maximum sooner in the hepatocytes in the experimental animals than in the controls. It is suggested that the stimulating effect of prodigiosan is connected with activation of the Kupffer cells of the liver.Laboratory of Pathophysiology, Institute of Clinical and Experimental Medicine, Siberian Branch, Academy of Medical Sciences of the USSR, Novosibirsk. (Presented by Academician of the Academy of Medical Sciences of the USSR V. P. Kaznacheev.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 86, No. 7, pp. 69–71, July, 1978.     

高山红景天多糖对小鼠抗柯萨奇B5病毒感染能力的研究

对感染柯萨奇Ｂ５病毒（ＣＶＢ）小鼠模型研究结果表明，高山红景天多糖对发病小鼠心肌功能改善和免疫功能提高均具有明显的促进作用。增强了小鼠抗ＣＶＢ５感染的能力，并对由ＣＢＶ感染性疾病也有一定的防治作用。     

Type IV collagenases in invasive tumors

Summary The matrix metalloproteinases appear to be elevated in tumors with metastatic potential, and may well be involved in penetration of the basement membrane and degradation of extracellular proteins including type IV collagen. An imbalance between the 72 kDa and 92 kDa type IV collagenases and the associated tissue inhibitors of these metalloproteinases (TIMPs) may therefore have a role in the invasive phenotype. Cultured tumor cells with invasive potential secrete both type IV collagenases, though in tumors there is some evidence that the 72 kDa form at least may be produced by stromal cells at the invading tumor front rather than primarily by the tumor cells themselves, while the 92 kDa form may be synthesized in macrophages near the front. These collagenases are elevated in invasive as compared within situ tumor components, but their specific roles and prognostic significance are not yet established.     

A comparison of the haemodynamic and hormonal effects of low and conventional dose cyclopenthiazide in normal volunteers

Summary In this study we compared low (125 g) and conventional (500 g) doses of cyclopenthiazide on the renin angiotensin system, plasma and extracellular fluid volumes and the pressor responsiveness to angiotensin II since we have previously shown that the two doses have the same antihypertensive effect but different effects on plasma renin activity.Following a two week placebo run-in period, 8 healthy male volunteers received 125 g or 500 g of cyclopenthiazide for 2 treatment periods of 4 weeks as part of a double blind, 2-part crossover study with treatment periods separated by a 4-week placebo washout phase. Measurements were made on two study days at the beginning and end of the active treatment periods. On the first day serum potassium, plasma renin activity and plasma angiotensin II levels were measured after a 1 h period of supine rest. Plasma and extracellular fluid volumes were also measured after appropriate equilibration times. The blood pressure responses to angiotensin II were assessed on day 2.The 500 g dose of cyclopenthiazide had a greater effect than the 125 g dose on plasma renin activity, serum potassium, angiotensin II levels and extracellular fluid volumes. Neither drug had any effect on plasma volume or the responsiveness to infused angiotensin II.Low dose cyclopenthiazide failed to increase angiotensin II levels, contract body fluid volumes or attenuate vascular reactivity in normotensive volunteers.     

肝细胞生长因子对多囊肾病囊肿衬里上皮细胞增殖及细胞外基质合成的影响

目的：研究重组人肝细胞生长因子（rhHGF）对常染色体显性遗传型多囊肾病（ADPKD）囊肿衬里上皮细胞增殖及细胞外基质合成的影响。方法：用^3H-TdR掺入法测定不同浓度（0．5、1、2．5、5ng/ml)rhHGF作用48h以及最适浓度的rhHGF分别作用24、48、72h ADPKD囊肿衬里上皮细胞系细胞的增殖情况;并分别用^3H-脯氨酸掺入法和放免法测定最适浓度rhHGF作用最佳时间后ADPKD囊肿衬里上皮细胞系细胞胶原及层粘连蛋白的合成情况。结果：rhHGF促进了ADPKD囊肿衬里上皮细胞增殖及胶原和层粘连蛋白的合成,以1ng/ml持续作用48h最为显。结论：rhHGF对体外培养的ADPKD囊肿衬里上皮细胞增殖及细胞外基质合成有明显的促进作用。     

肝癌组织中TIMP-1和TIMP-2的表达意义

目的 了解TIMP－1和TIMP－2在肝癌组织中的表达状态,探讨TIMP－1和TIMP－2在肝癌组织生长、侵润及转移中所起的作用。方法 以抗TIMP－1和TIMP－2单克隆抗体（mAb)为试剂,采用免疫组织化学法检测原发性肝癌、肝高分化腺癌的肝组织中TIMP－1和TIMP－2的表达,并与10例正常肝组织做对照。结果 10例原发性肝癌患的肝组织中TIMP－1和TIMP－2蛋白表达的阳性率为100％,在癌组织及非癌组织中均有表达。癌组织中的TIMP－1和TIMP－2蛋白表达的强度比较为6例高于、4例低于周围的非癌组织（慢性肝炎及肝硬化组织）,癌组织中TIMP－1和TIMP－2蛋白的表达呈散在性分布,但TIMP－1比TIMP－2表达强。9例肝高分化腺癌的腺癌组织中无TIMP－1和TIMP－2相关抗原的表达,但癌周组织的肝细胞有3例TIMP－1和TIMP－2蛋白表达为阳性。10例正常肝组织中TIMP－1和TIMP－2蛋白表达均为阴性。结论 TIMP－1和TIMP－2存在于原发性肝癌的癌组织中,其表达的部位与强度可能与癌的生长、浸润及转移有关。     

灵芝菌发酵培养基的优化及灵芝胞外多糖的分离纯化

用正交法研究了不同条件对灵芝菌生长影响,从中选出了灵芝 菌液体深层发酵的优化 培养基：葡萄糖 3.6%,蛋白胨 0.4%,pH 6.0,酵母膏 0.2%,KH2PO40.1%,MgSO 4· 7H2O 0.05%,Vit B1 0.005%,每升发酵液产干菌丝体 15.6 g,粗多糖 0.72 g。 经葡 聚糖凝胶层析对灵芝胞外多糖进行了分离纯化,共有 5 个组分,并用红外光谱、紫外光谱 ,气相色谱等手段进一步分析了灵芝胞外多糖的组成,结果表明灵芝胞外多糖系由甘露糖、 果糖、葡萄糖通过糖 β-D 糖苷键构成。     

南柴胡多糖分离与组成的初步研究

南柴胡粗多糖经糖腈乙酰酯化后采用高分辨毛细管气相色谱分析,发现南柴胡粗多糖主要由Ｌ－阿拉伯糖、核糖、Ｄ－木糖、Ｄ－甘露糖、Ｄ－葡萄糖、Ｄ－半乳糖等单糖组成,校正因子法测得它们的摩尔比为０．１０６：２．６５２：４．０７０：０．５１９：０．９３０：２．５１８。     

不同细胞外基质在大鼠胚胎心脏中表达规律的比较

目的比较不同细胞外基质(ECM)及其共同基质受体在大鼠胚胎心脏中的表达规律,探讨ECM在胚胎期心脏发生、发育过程中的作用。方法将胎龄天数(ED)为11～19d的胚鼠用酶免疫组织化学染色检测ECM中的FN、LMN、Vn、CoⅣ及其受体β1在胚胎心脏中的表达。结果①ECM及其受体在胚胎各期心脏的空间性分布各不相同,FN及LMN有明显的空间分布互补关系,β1的空间分布主要与FN相似,但在空间隔及心外膜中又与LMN相似;②FN、LMN及β1在不同心脏部位的表达高峰及持续时间各不相同,β1的表达高峰要早于FN及LMN。结论ECM(主要是FN与LMN)及其受体β1在胚胎心脏的发生发育中起着一定的介导作用,其作用的时期、部位及作用机制各不相同。     

冬虫夏草对肾衰患者细胞氨基酸代谢的影响

目的 研究冬虫夏草（虫草）对曼性肾功能衰竭（ＣＲＦ）患者细胞内外氨基酸代谢的影响。方法 采且ＬＫＢ－４４００型氨基酸自动分析仪测定１０例ＣＲＦ患者服用虫草前后的血浆、红细胞、骨骼肌细胞内的游离氨基酸浓度。结果 ＣＲＦ患者服用虫草后血浆中亮氨酸、异这氨酸、苏氨酸、赖氨酸、胱到、酷氨酸浓度和红细胞中酷氨酸、谷氨酸浓度接近正常,１例骨骼肌标本苏氨酸、赖氨酸浓度接近正常;而细胞内外缬氨酸浓度仍显著降低。结