组织工程真皮抗张强度的初步研究

目的 采用高敏度小张力膜状生物材料力学性能测定仪检测组织工程真皮的抗张强度。方法 分别培养含有不同数量成纤维细胞的胶原凝胶和复方壳多糖真皮替代物,15d后检测抗张强度。结果 组织工程真皮抗张强度随成纤维细胞数量增多而增强（P〈0．01）,复方壳多糖人工真皮的抗张强度高于胶原凝胶真皮（P〈0.01）。结论 复方壳多糖真皮替代物具有良好的抗张强度,为临床应用提供稳定机械性能。     

胶原-壳聚糖载硫酸长春新碱微球缓释药膜的研究

目的本研究制备载硫酸长春新碱(vincristinesulfate,VCR)微球的胶原-壳聚糖缓释药膜。并考察加入壳聚糖对药膜性质的影响。选定适当的胶原壳聚糖比例制备药膜。方法采用W/O/O溶剂挥发法制备VCR的聚乳酸-羟基乙酸(poly(lactic-co-glycolicacid),PLGA)微球,并对微球性质表征,采用二次冻干法制备载VCR微球的胶原-壳聚糖药膜,对药膜的表面形态、降解性质、热力学性质及释放性质进行表征,并与释放2周后的药膜进行比较。采用高效液相法分析药物含量。结果VCR制成PLGA微球后再制备成药膜,可达到双重缓释的作用,明显减少药物突释,并延缓药物释放。添加了壳聚糖的药膜降解速度明显小于单纯的胶原药膜。在体外释放实验中,微球突释为(27.2±1.2)%,而胶原药膜的突释为(20.4±1.9)%,胶原与壳聚糖比例为9∶1、4∶1、3∶2的药膜突释分别为(20.2±2.1)%、(18.0±1.1)%和(16.3±1.8)%。结论胶原壳聚糖载VCR的缓释药膜能不同程度减少药物的突释,使药物释放更加平稳缓慢,优于单纯的胶原药膜。     

Sustainable Chitosan-Dialdehyde Cellulose Nanocrystal Film

In this study, we incorporated 2,3-dialdehyde nanocrystalline cellulose (DANC) into chitosan as a reinforcing agent and manufactured biodegradable films with enhanced gas barrier properties. DANC generated via periodate oxidation of cellulose nanocrystal (CNC) was blended at various concentrations with chitosan, and bionanocomposite films were prepared via casting and characterized systematically. The results showed that DANC developed Schiff based bond with chitosan that improved its properties significantly. The addition of DANC dramatically improved the gas barrier performance of the composite film, with water vapor permeability (WVP) value decreasing from 62.94 g·mm·m⁻²·atm⁻¹·day⁻¹ to 27.97 g·mm·m⁻²·atm⁻¹·day⁻¹ and oxygen permeability (OP) value decreasing from 0.14 cm³·mm·m⁻²·day⁻¹·atm⁻¹ to 0.026 cm³·mm·m⁻²·day⁻¹·atm⁻¹. Meanwhile, the maximum decomposition temperature (Td_max) of the film increased from 286 °C to 354 °C, and the tensile strength of the film was increased from 23.60 MPa to 41.12 MPa when incorporating 25 wt.% of DANC. In addition, the chitosan/DANC (75/25, wt/wt) films exhibited superior thermal stability, gas barrier, and mechanical strength compared to the chitosan/CNC (75/25, wt/wt) film. These results confirm that the DANC and chitosan induced films with improved gas barrier, mechanical, and thermal properties for possible use in film packaging.     

Thermal Stability and Dynamic Mechanical Properties of Poly(ε-caprolactone)/Chitosan Composite Membranes

Poly (ε-caprolactone) (PCL) and chitosan (CS) are widely used as biodegradable and biocompatible polymers with desirable properties for tissue engineering applications. Composite membranes (CS–PCL) with various blend ratios (CS:PCL, w/w) of 0:100, 5:95, 10:90, 15:85, 20:80, and 100:0 were successfully prepared by lyophilization. The thermal stabilities of the CS–PCL membranes were systematically characterized by thermogravimetric analysis (TG), dynamic thermogravimetry (DTG), and differential scanning calorimetry (DSC). It was shown that the blend ratio of PCL and CS had a significant effect on the thermal stability, hydrophilicity, and dynamic mechanical viscoelasticity of the CS–PCL membranes. All the samples in the experimental range exhibited high elasticity at low temperature and high viscosity at high temperatures by dynamic mechanical thermal analysis (DMTA). The performances of the CS–PCL membranes were at optimum levels when the blend ratio (w/w) was 10:90. The glass transition temperature of the CS–PCL membranes increased from 64.8 °C to 76.6 °C compared to that of the pure PCL, and the initial thermal decomposition temperature reached 86.7 °C. The crystallinity and porosity went up to 29.97% and 85.61%, respectively, while the tensile strength and elongation at the breakage were 20.036 MPa and 198.72%, respectively. Therefore, the 10:90 (w/w) blend ratio of CS/PCL is recommended to prepare CS–PCL membranes for tissue engineering applications.     

生物可降解包装膜

以明胶和壳聚糖作为成膜介质 ,探讨了交联剂质量浓度、pH、增塑剂质量浓度、成膜温度、成膜材料质量浓度等对膜的抗拉强度、穿刺强度和水溶性的影响 ,得到了具有良好抗水抗油能力并具有一定强度的明胶膜和壳聚糖膜 .     

不同虾壳为原料的甲壳素与壳聚糖的制备

目的 :采用两种虾壳为原料制备甲壳素与壳聚糖 ,为内地开展甲壳素与壳聚糖的研究提供可行的原料制备方法。方法 :将收集的虾壳处理干净 ,用 2mol·L-1盐酸浸泡 ,除去虾壳中钙质 ,10 %NaOH煮沸除去虾壳中的蛋白质 ;4 0 %NaOH煮沸 ,脱去甲壳素的部分乙酰基 ,制得壳聚糖。结果 :两种虾壳制备甲壳素收率分别为 15 .92 % ,17.2 5 % ;制备壳聚糖的收率分别为 10 .84 % ,11.78% :由甲壳素制备壳聚糖的收率均为 6 8%。制备的壳聚糖均溶于 1%HAc。结论 :制备方法稳定、可行 ,可以采用本方法 ,利用废弃的虾壳制备甲壳素、壳聚糖     

壳聚糖缓释给药系统植入脉络膜上腔治疗兔眼穿通伤

目的：比较壳聚糖缓释给药系统植入脉络膜上腔与球结膜下给药联合全身给药治疗兔眼穿通伤的实验疗效。方法：24只青紫蓝兔双眼制作成实验性眼外伤模型,采用电脑随机分为2组,A组给以壳聚糖缓释给药系统(载药曲安奈德)植入脉络膜上腔、B组球结膜下注射联合肌肉注射,经处理后行视网膜脱离及玻璃体浑浊评级、眼底造影。结果：A组抑制炎症和增殖反应的效果优于B组。结论：壳聚糖缓释给药系统(载药曲安奈德)植入脉络膜上腔安全有效,是治疗穿通伤的较好的新的方法。     

可注射温固化支架复合异体软骨细胞修复关节软骨缺损的初步研究

目的探讨可注射性温固化凝胶壳聚糖-甘油磷酸钠（C-GP）复合同种异体软骨细胞在体内形成透明软骨和注入关节腔后修复关节软骨缺损的可行性和有效性。方法将预制的壳聚糖和甘油磷酸钠按一定体积比制成混合液,实验组为支架复合同种异体软骨细胞,设单纯支架和生理盐水为两组对照。注入成年兔的背部皮下和预制关节软骨缺损的关节腔内,复合物在体内37℃形成凝胶,术后4、8、12周分别行大体、组织学（HE、TB）、Ⅱ型胶原免疫组织化学观察,并进行Wakitani评分。观察其体内成软骨和填充并修复关节缺损效果。结果液态C-GP复合物注入体内可形成凝胶,实验组皮下生长4周,组织学观察示典型的透明软骨样结构且分泌基质;C-GP复合细胞注入关节腔可很好地填充关节缺损,并于4周后开始形成透明软骨样结构且表面平整与宿主整合良好,组织学切片上可见类软骨形成并分泌甲苯胺蓝异染的软骨基质和软骨特异性Ⅱ型胶原。结论可注射性C-GP复合软骨细胞体内可形成具有一定形态功能的类软骨组织,能够应用于微创技术再生修复软骨的缺损。     

葛根素-壳寡糖共无定型制备与体外溶出度评价

目的 提高葛根素的生物利用度.方法 采用低温研磨法制备葛根素-壳寡糖共无定型(PUE-COS CM),采用差示扫描量热法、X射线粉末衍射法、傅里叶红外光谱法、扫描电镜法对PUE-COS CM进行固态表征分析;评价PUE-COS CM在漏槽和非漏槽2种条件下的体外溶出行为,并考察其稳定性.结果 差示扫描量热法证实,PUE...     

中药肌能饮联合壳聚糖季铵盐硅凝胶对瘢痕疙瘩修复的观察

目的探讨中药肌能饮联合壳聚糖季铵盐硅凝胶对瘢痕疙瘩修复的效果。方法 98例瘢痕疙瘩患者使用该方法治疗后3个月,按照显效、有效和无效标准进行疗效分析。结果用中药肌能饮联合壳聚糖季铵盐硅凝胶治疗瘢痕疙瘩患者3个月后,瘢痕疙瘩修复的有效率为93.9%,未见不良反应。3个月后随访,患者情况基本稳定。结论中药肌能饮联合壳聚糖季铵盐硅凝胶对瘢痕疙瘩的修复效果好,安全性高。