S-8型大孔树脂对葛根素的吸附研究(英文)

利用S-8树脂分离葛根提取液中的葛根素,以S-8型大孔树脂对葛根素的吸附行为为研究对象,对葛根素的吸附平衡和吸附动力学进行了研究,发现S-8树脂对葛根素的吸附可以用Langmuir等温吸附方程和Freundlich等温吸附方程来描述,动力学研究表明实验所取初始浓度范围下S-8吸附葛根素的吸附速率由液膜控制,遵循Boyd液膜扩散模型。同时,进行动态吸附解吸实验,在室温(25°C)下,洗脱液为80%的乙醇时,洗脱效果较好,洗脱率为84%,一次纯化后所得产品中葛根素纯度为43.75%。     

良药葛根的临床应用

从经方配伍、临床运用、药理研究、服药方法及不良反应等角度出发,对葛根药用的研究进展做了全面的阐述,可作为今后葛根研究的参考。     

山西产葛根总黄酮及3种异黄酮含量的测定

目的:测定山西产葛根总黄酮及3种异黄酮的含量。方法:紫外分光光度法测定葛根总黄酮的含量,检测波长250nm。高效液相色谱法测定葛根3种异黄酮的含量,色谱柱为Uvis-201(4.6mm×250mm,5μm),流动相为甲醇(A)-水(B)梯度洗脱,流速为1.0mL/min,柱温为室温;检测波长250nm。结果:葛根总黄酮在4.32-21.60μg/mL范围内线性关系良好,平均回收率为99.20%,RSD为1.57%;葛根素、大豆苷、大豆苷元的进样量分别在0.2124-4.2480μg、0.1207-2.4140μg、0.0883-1.7660μg范围内呈良好的线性关系,平均回收率分别为99.96%、99.02%、99.12%,RSD分别为1.68%、2.50%、2.19%。结论:本方法简单准确,重复性好,适用于葛根总黄酮及3种异黄酮的含量测定。     

基于体外心肌细胞活力的葛根提取工艺优选

目的:优选葛根药材的提取工艺.方法:采用正交试验法,选取提取时间、料液比、提取次数及乙醇体积分数为影响因素,以葛根素含量和药效学的体外心肌细胞活力为指标综合分析,优选提取工艺条件.结果:葛根的乙醇提取最佳工艺条件为加6倍量70％乙醇回流提取2次,每次1.5h.结论:化学指标结合药效学指标综合分析葛根的提取纯化工艺可较好地保证制剂的质量,且该优选工艺稳定可行.     

响应面分析法优化葛根中异黄酮提取工艺

目的:优选葛根中异黄酮的提取工艺.方法:以葛根异黄酮为评价指标,通过响应面分析法优化葛根异黄酮的提取工艺,并对最佳提取工艺进行工艺验证.结果:葛根异黄酮的最佳提取工艺为65％乙醇,超声提取28 min,料液比1∶29,异黄酮的提取率可达4.87％.结论:采用响应面分析法优化对葛根异黄酮提取条件进行优化合理可行.     

星点设计-效应面法优化葛根素提取工艺

目的:采用星点设计-效应面法优化葛根素提取过程中的有关工艺参数。方法:以提取时间、料液比为考察因素,葛根素提取率为指标,分别用多元线性模型和二次多项式模型描述考察指标和两个考察因素之间的数学关系,绘制效应面图,确定较优参数并进行验证实验。结果:确定的最佳提取工艺为:料液比1∶8.88(W/V),提取时间为150 min;二项式拟合的相关系数为0.9977,提取率预测值与理论值偏差0.59%。结论:实验结果与模型预测值相符,该工艺可用于葛根中葛根素的提取。     

史载祥治疗难治性发热三法

难治性发热病机较为复杂,肿瘤发热在难治性发热中具有代表性.史载祥教授认为,运用经方治疗难治性发热的关键在于精准辨证.临证时当从整体出发,明确邪正关系,处方之时当谨守病机,方证对应,既遵循中医学整体观念、辨证施治的基本特点,又强调更为精准的辨证与个体化治疗.史教授创立清解三阳退热法、培土生金宣热法、灌肠逐瘀导热法,治疗难...     

Efficacy and Safety of Pueraria lobata Extract in Gray Hair Prevention: A Randomized,Double-Blind,Placebo-Controlled Study

Background

Graying of hair-a sign of aging-raises cosmetic concerns. Individuals with gray hair often look older than others their age; therefore, some dye their hair for aesthetic purposes. However, hair colorants can induce many problems including skin irritation, allergic reaction and hair-breakage.

Objective

This randomized, double-blind clinical trial was performed in order to examine the effects of APHG-1001, a compound including an extract from Pueraria lobata, on graying hair.

Methods

A total of 44 female subjects were randomly treated with either APHG-1001 or placebo twice daily for 24 weeks. Using the phototrichogram analysis, a count of newly developed gray hair was estimated. Investigator assessment and subject self-assessment were also performed in order to evaluate the efficacy of the compound.

Results

The mean number of newly developed gray hair at 24 weeks was 6.3/cm² in the APHG-1001 group and 11.4/cm² in the placebo group; the difference was statistically significant (p<0.05). However, the investigator assessment and subject self-assessment did not show any significant change in the gross appearance of hair grayness by the end of the study. No severe adverse events in either group were observed. Moreover, the incidence of adverse events did not differ between the groups.

Conclusion

This clinical trial revealed that APHG-1001, which contains an extract of P. lobata, could prevent the development of new gray hair without any remarkable adverse effects. Thus, it can be considered as a viable treatment option for the prevention of gray hair.     

大孔吸附树脂分离纯化葛根总黄酮和葛根素的工艺优选

目的:优选大孔吸附树脂分离、纯化葛根总黄酮和葛根素的工艺。方法:考察AB-8,D-101,HPD-100,S-8 4种不同极性吸附树脂对葛根中总黄酮和葛根素的静态吸附及解吸性能,筛选树脂型号;以葛根素、总黄酮转移率和纯度为指标,通过单因素试验考察其吸附和洗脱条件。结果:选用AB-8型大孔吸附树脂,优选的工艺条件为上样液质量浓度1.0 g.mL-1,葛根与大孔树脂质量比1∶2,径高比1∶6,用2 BV水洗除杂,加2 BV 80%乙醇以2 mL.min-1洗脱。转移率均>92%,所得葛根总黄酮纯度达80%,葛根素质量分数25%。结论:AB-8型大孔树脂用于富集葛根总黄酮和葛根素效果最佳,是一种理想的分离纯化介质,且优选的工艺操作简单、方法可靠。     

葛根散对酒精性脂肪肝大鼠肝功能损伤的影响

目的：观察葛根散对酒精性脂肪肝大鼠肝功能损伤的影响。方法将100只Wistar雄性大鼠随机分为6组,正常对照组、模型对照组、联苯双酯滴丸组及葛根散高、中、低剂量组。正常对照组正常饲养,其余5组建立酒精性脂肪肝大鼠模型。葛根散高、中、低剂量组分别以24．84、12．42、6．21 g/kg灌胃给药,联苯双酯滴丸组以3．6 mg/kg灌胃给药,正常对照组与模型对照组以0．9％氯化钠注射液10 mL/kg灌胃给药。均每日1次,连续灌胃30 d。比较大鼠血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）及总胆红素（ TBiL）水平变化,并观察各组大鼠肝组织的病理变化。结果与正常对照组比较,模型对照组大鼠血清ALT、AST及TBiL水平均显著升高（P＜0．05）;与模型对照组比较,葛根散高、中、低剂量组及联苯双酯滴丸组血清ALT、AST及TBiL水平均有明显降低（P＜0．05）,且葛根散高剂量组降低水平与联苯双酯滴丸组相当（P＞0．05）,并优于葛根散中、低剂量组（P＜0．05）。结论葛根散可显著降低酒精性脂肪肝模型大鼠血清ALT、AST及TBiL水平,其药物疗效作用强度具有一定的剂量依赖关系,对酒精性脂肪肝模型大鼠肝损伤有保护作用。