Hypoglycemic and antioxidant effects of Rheum franzenbachii extract in streptozotocin-induced diabetic rats

The hypoglycemic and antioxidant effects of ethanol extract from the roots and rhizomes of Rheum franzenbachii Münt. (Polygonaceae) were evaluated in streptozotocin-induced diabetic rats. Effects of repeated oral administration of ethanol extract (125, 250, and 500?mg/kg body weight) on the plasma glucose level (PGL), oral glucose tolerance test (OGTT), malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT) in diabetic rats were examined. It was found that administration of ethanol extract (125, 250, and 500?mg/kg) produced a significant fall in PGL, AUC, and MDA, while elevating the GSH levels and SOD and CAT activities in diabetic rats. The dose of 500?mg/kg was identified as the most effective dose, with a decrease of 65.8 and 44.0% in PGL and MDA, and elevation of 72.6, 75.0, and 51.5% in GSH level and SOD and CAT activities, respectively, after 14 days of ERF administration in diabetic rats. Moreover, the OGTT studies showed a maximum reduction in PGL and AUC. From the active extract of Rheum franzenbachii, two stilbenes, desoxyrhapontigenin (1) and desoxyrhaponticin (2), were isolated as major constituents. The present study concludes that the ethanol extract of roots and rhizomes from Rheum franzenbachii had significant hypoglycemic and antioxidant effects.     

唐古特大黄化学成分研究

目的研究蓼科大黄属植物唐古特大黄Rheum tanguticum根的化学成分。方法采用硅胶和凝胶柱色谱方法进行分离,经核磁和质谱等波谱分析方法鉴定化合物结构。结果分离得到16个化合物,分别鉴定为大黄酚(1)、大黄素(2)、大黄素甲醚(3)、大黄酚-1-O-β-D-葡萄糖苷(4)、大黄酸(5)、芦荟大黄素8-O-β-D-吡喃葡萄糖苷(6)、大黄素8-O-β-D-吡喃葡萄糖苷(7)、林氏莲花掌素(8)、4-(4′-对羟基苯基)-2-丁酮-4′-O-β-D-葡萄糖苷(9)、白黎芦醇4′-O-β-D-吡喃葡萄糖苷(10)、白黎芦醇4′-O-β-D-(6″-O-没食子酰)-吡喃葡萄糖苷(11)、6-羟基酸模素-8-O-β-D-吡喃葡萄糖苷(12)、表儿茶素-3-O-没食子酸酯(13)、儿茶素(14)、对羟基苯丙烯酸葡萄糖酯(15)、对羟基苯甲酸葡萄糖酯(16)。结论化合物15、16为首次从唐古特大黄中分离得到。     

藏边大黄的化学成分研究

研究藏边大黄的化学成分。方法 用硅胶、聚酰胺柱色谱分离结合重结晶方法进行分离纯化。用MS、1H-NMR、13C-NMR谱学方法结合化学方法确定化合物的结构。结果 从藏边大黄干燥根与根茎的乙醇提取物中分得18个化合物,鉴定了其中16个化合物的结构,分别为大黄酚(1)、大黄素甲醚(2)、β-谷甾醇(3)、大黄素(4)、芦荟大黄素(5)、波叶素(6)、胡萝卜苷(7)、d-儿茶素(8)、反-3,5,3′,4′-四羟基茋(9)、反式-3,5,3′,4′-四羟基茋-4′-O-β-D-吡喃葡萄糖苷(10)、反式-3,5,3′,4′-四羟基茋-4′-O-β-D-(6″-O-p-香豆酰)-吡喃葡萄糖苷(11)、大黄酚-8-O-β-D-吡喃葡萄糖苷(12)、大黄素甲醚-1和8-O-β-D-吡喃葡萄糖苷(13a和13b)、大黄素-8-O-β-D-吡喃葡萄糖苷(14)、蔗糖(15)。同时得到一种大黄酸类似物,因量少未得到最终的结构确定。结论 化合物11为一新化合物,命名为波叶苷(rheoside),化合物13a和13b系首次从该植物中分得。     

AFLP分析唐古特大黄、掌叶大黄和药用大黄的亲缘关系研究

目的研究《中国药典》2005年版收载蓼科大黄属3种大黄(唐古特大黄、掌叶大黄和药用大黄)的亲缘关系。方法对3种大黄44份样品进行AFLP分析,同时利用NTSYSpc-2.11F软件分析其亲缘关系。结果8对选择性扩增引物中,扩增得到1 255条稳定清晰的条带,其中多态性条带1 209条,多态性带占总带数的96.2%。将44份大黄样品分为2组2个亚组。四川若尔盖唐古特大黄聚为第一组,第二组分为两个亚组,分别为药用大黄和掌叶大黄。另外,大黄的AFLP分子标记谱带显示,3种大黄和不同来源的大黄样品间除了具有共有带外,还有一些特异带,说明种间甚至不同来源的样品间有一定程度的特异性。结论《中国药典》规定的3种正品大黄中,药用大黄和掌叶大黄亲缘关系较唐古特大黄近,表明利用AFLP技术分析3种大黄的亲缘关系可行。     

Analgesic and diuretic properties of α‐santalone from Polygonum flaccidum

Polygonum flaccidum Meissn. is an annual herb, native to Bangladesh, and well known for its analgesic, anti‐inflammatory, diuretic, purgative and insecticidal properties, and also for its use against snake‐bites. The analgesic and the diuretic properties of α‐santalone (1), isolated from the aerial parts of Polygonum flaccidum, were assessed by the acetic‐acid‐induced writhing method and the Lipschitz test, respectively. Complete ¹H and ¹³C NMR data of 1 are also presented. Copyright © 2010 John Wiley & Sons, Ltd.     

卷茎蓼地上部分化学成分研究

目的:研究蓼科何首乌属植物卷茎蓼Fallopia convolvulus地上部分的化学成分,为蓼科蓼族的化学分类学研究提供物质基础。方法:以80%乙醇热回流提取,采用硅胶、凝胶柱色谱等多种柱色谱技术以及重结晶等方法进行分离,并根据理化性质和波谱数据进行结构鉴定。结果:共分离鉴定了12个化合物,分别为槲皮素(1),木犀草素(2),金圣草素(3),芹菜素(4),山柰酚-3-O-β-D-葡萄糖苷(5),槲皮素-3-O-鼠李糖苷(6),N-反式-阿魏酰酪胺(7),loliolide(8),对羟基苯甲酸乙酯(9),β-谷甾醇(10),胡萝卜苷(11)和正十六烷酸(12)。结论:以上化合物均为首次从卷茎蓼中分得,其中化合物6~9为首次从该属植物中发现。     

愉悦蓼的黄酮类成分研究

目的:研究蓼科蓼属植物愉悦蓼Polygonum jucundum全草的化学成分,为研究蓼属化学分类学提供物质基础.方法:以85%乙醇热回流提取,采用硅胶、凝胶柱色谱等多种色谱技术以及重结晶等方法分离纯化化合物,根据理化性质和波谱数据进行结构鉴定.结果:共分离鉴定了8个化合物,分别为槲皮素-3'-O-β-D-半乳糖苷(1),8-甲氧基槲皮素(2),芹菜素(3),木犀草素(4),槲皮素(5),3,5,7.三羟基色原酮(6),对羟基苯甲醛(7)和β-谷甾醇(8).结论:以上化合物均为首次从该种植物中分得,其中化合物1,2,6～8为首次从蓼属植物中发现.     

荭草化学成分研究Ⅱ

目的:研究荭草Polygonum orientale有效部位的化学成分.方法:应用色谱技术分离纯化,根据化合物的理化性质和波谱数据鉴定其结构.结果:分离并鉴定了7个化合物,分别为商陆素-3-O-β-D-半乳糖苷(ombuine-3-O-β-D-galactopyranoside,1),商陆素-3-O-芸香糖苷(ombuine-3-O-rutinoside,2),色氨酸(tryptophan,3),槲皮素-3-甲醚(quercetin-3-O-methyl ether,4),山奈酚-3-O-(2"-O-α-L-鼠李糖基)-β-D-葡萄糖醛酸苷[kaempferol-3-O-(2"-O-α-L-rhamnopyranosyl)-β-O-glucuronopyranoside,5],槲皮素3-O-(2"-O-α-L-鼠李糖基)-β-D-葡萄糖醛酸苷[quercetin-3-O-(2"-O-α-L-rhamnopyranosyl)-β-D-glucuronopyranoside,6],槲皮素-3-O-β-D-葡萄糖醛酸苷(quercetin-3-O-β-D-glucumnide,7).结论:化合物4～7为首次从该种植物中分离得到,化合物1～3为首次从该属植物中分离得到,并首次归属化合物1的1H和13C-NMR谱信号.     

包茎拳参中一个新的抗氧化剂5,6-dihydropyranobenzopyrone(英文)

AIM: To study the chemical constituents and their bioactivity of Polygonum amplexicaule. METHODS: The isolation of compounds was achieved by chromatographic techniques and structure of the isolates was established by UV, IR, HRESI-MS and NMR including 1D and 2D experiments. RESULTS: Bioassay-guided fractionation of an ethanolic extract of Polygonum amplexicaule led to the isolation of a hitherto unidentified compound, 5, 6-dihydropyranobenzopyrone (1) along with nine previously known com-pounds (2-10). Compounds 2-10 were identified as amplexicine (2), catechin (3), rutin (4), quercetin-3-O-β-D-galactopyranoside (5), chlorogenic acid (6), galloyl glucose (7), caffeic acid (8), gallic acid (9) and scopletin (10). CONCLUSION: Compound 1 is new. Compounds 1-10 exhibited considerable antioxidant activity in a 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay.     

Anthraquinone and stilbene derivatives from the cultivated Korean Rhubarb Rhizomes

The studies were carried out to evaluate the constituents in the rhizomes of the cultivated Korean Rhubarb (Polygonaceae). From the acetone fraction of methanol extract Compound 1 (1,8-dihydroxy-3-methyl anthraquinone, chrysophanic acid), Compound II (chrysophanol-8-O-β-D-glucopyranoside), Compound III (emodin-8-O-β-D-glucopyranoside) and Compound IV (aloe-emodin-8-O-β-D-glucopyranoside), and from the ether fraction Compound V (1,8-dihydroxy-3-methyl-6-methoxy anthraquinone, physcion) and Compound VI (1,6,8-trihydroxy-3-methyl anthraquinone, emodin), and also from the n-buthanol fraction Compound VII (rhapontigenin-3-O-β-D-glucopyranoside, rhaponticin) and Compound VIII (piceatannol-3′-O-β-D-glucopyranoside), were isolated and identified on the basis of their physico-chemical and spectroscopic evidences (UV, IR,¹H-NMR,¹³C-NMR, El-MS), respectively.