胃复春片对慢性胃病患者窦D细胞的影响

目的：探讨胃复春片治疗慢性胃病患者前后生长抑素内分泌细胞(D细胞)数量变化。方法：用免疫组化酶链亲合素过氧化酶复合技术(SABC)对患者治疗前后胃粘膜组织的D细胞染色，观察治疗前后D细胞的形态和数量变化。结果：经胃复春片治疗后，患者胃窦粘膜D细胞阳性数及强阳性率均明显下降，有显著意义。结论：胃复春片对慢性胃病患者生长抑素D细胞有调整作用。     

Usefulness of antiendomysial antibodies as a serological marker in coeliac children

The new diagnostic criteria of coeliac disease (CD) give more importance to serological markers. Immunoglobulin A antiendomysial antibodies (IgA-EmA) were determined in 138 sera from 79 coeliac children and the antibody levels compared to IgG and IgA antigliadin antibodies (IgG-AGA, IgA-AGA) in the sera. The assessment was also carried out in 29 children with other gastrointestinal diseases, 29 with non-gastrointestinal diseases and 35 healthy children. The IgA-EmA had a 91.4% specificity and a 88.4% sensitivity for active CD. The corresponding figures were 89.8% and 64.4% for IgA-AGA and 73.7% and 86.2% for IgG-AGA, respectively. The results of IgA-EmA determinations were concordant with the intestinal biopsy findings in 90% of cases, versus 80% for IgA-AGA and 83% for IgG-AGA. In most of the discordant cases the biopsy showed only minor changes, making the classification difficult. All patients with positive IgA₂-EmA also had positive IgA₁ EmA antibodies. IgA-EmA are an excellent serological marker of CD activity in children and they are useful to decrease the number of intestinal biopsies which are needed to confirm the diagnosis in coeliac patients.     

应用VIDAS免疫分析系统检测血清抗伯氏包柔螺旋体抗体

莱姆病（Ｌｙｍｅｄｉｓｅａｓｅ）是最近十年来才被认识的一种蜱媒传染流行病，Ｌｙｍｅ病的血清学诊断方法有间接荧光免疫法（ＩＦＡ），酶免疫试验（）ＥＩＡ），免疫印迹法（ＷＢ），梅里埃生物公司（Ｂｉｏｍｅｒｉｅｕｘ）推出ＶＩＤＡＳ免疫诊断系统能够自动定性和半定量地检查Ｌｙｍｅ病的抗伯氏包柔螺旋体抗（抗ＢＢ抗体）。本方法结合了酶和荧光免疫两种方法。硷性磷酸酶标记二抗的底物是４ｍｅｔｈｙｌｕｍｂｅｌｌｉｆｅ     

Myocardial metabolic imaging by means of fluorine-18 deoxyglucose/technetium-99m sestamibi dual-isotope single-photon emission tomography

The detection of preserved glucose uptake in hypoperfused dysfunctional myocardium by fluorine-18 deoxyglucose (FDG) positron emission tomography (PET) represents the method of choice in myocardial viability diagnostics. As the technique is not available for the majority of patients due to cost and the limited capacity of the PET centres, it was the aim of the present work to develop and test FDG single-photon emission tomography (SPET) with the means of conventional nuclear medicine. The perfusion marker sestamibi (MIBI) was used together with the metabolic tracer FDG in dual-isotope acquisition. A conventional SPET camera was equipped with a 511-keV collimator and designed to operate with simultaneous four-channel acquisition. In this way, the scatter of 18F into the technetium-99m energy window could be taken into account by a novel method of scatter correction. Thirty patients with regional wall motion abnormalities at rest were investigated. The results of visual wall motion analysis by contrast cine-ventriculography in nine segments/heart were compared with the results of quantitative scintigraphy. The scintigraphic patterns of MIBI and FDG tracer accumulation were defined as normal, matched defects and perfusion-metabolism mismatches. Spatial resolution of the system was satisfactory, with a full width at half maximum (FWHM) of 15.2 mm for ¹⁸F and 14.0 mm for ^99mTe, as measured by planar imaging in air at 5 cm distance from the collimator. Image quality allowed interpretation in all 30 patients. 88% of segments without relevant wall motion abnormalities presented normal scintigraphic results. Seventy-five akinetic segments showed mismatches in 27%, matched defects in 44% and normal perfusion in 29%. We conclude that FDG-MIBI dual-isotope SPET is technically feasible with the means of conventional nuclear medicine. Thus, the method is potentially available for widespread application in patient care and may represent an alternative to the ²⁰¹T1 reinjection technique.     

上海人群发病、患病情况分析

该文对上海市急性传染性疾病和慢性非传染性疾病的发病与患病情况进行了描述分析 ,结果发现 :急性传染性疾病得到了有效控制 ,但是仍存在隐患 ;慢性非传染性疾病成为健康的主要威胁。同时提出了有关的政策建议。     

胚鼠黑质细胞悬液移植于帕金森病模型鼠侧脑室的实验研究

将胚鼠中脑腹侧组织制成细胞悬液移植于帕金森病(PD)模型鼠侧脑室,动态观察受体鼠在移植前后的行为变化。移植后16周应用诱发荧光及免疫细胞化学等方法,对移植物在受体鼠脑内的存活及生长情况进行了研究。结果,受体鼠旋转行为明显改善,侧脑室内可见黄绿色荧光细胞及酪氨酸羟化酶(TH)免疫反应阳性细胞,有的发出突起伸向室管膜及迁于尾核区.提示:移植的胚鼠黑质细胞生长良好,似作为触液神经元发挥其作用。     

A tactile maze test in unilateral spatial neglect: the influence of vision and recording technique

Summary Right brain-damaged patients with left spatial neglect were examined on a tactile maze task. They started to explore the right side of the maze and their search times were longer on the left side. Their performance was the same whether blindfolded or not, which contrasts with the results of other studies and probably reflects differences in task demand. The present results do not support a previous observation that visual neglect can be compensated through the tactile modality. Our patients had markedly longer search times than normal controls in the right as well as the left hemifield, and search times in both hemifields were positively correlated. The outcome of the maze test in this context may therefore depend to a large extent on impaired topo graphical processing.     

The postoperative recurrence of crohn's disease: An analysis of 37 patients with crohn's disease who underwent endoscopy during initial surgery

A total 37 patients with Crohn's disease who underwent intraoperative endoscopy during resection of the affected intestine were evaluated in this study. The average age of the patients at surgery was 23.2 years. The residual lesions in the remaining intestine identified by intraoperative endoscopy were classified according to their pathologic profiles into three groups: A, B and C. In group A, comprising patients with longitudinal ulcers and/or a cobblestone appearance, 10 of 12 patients had recurrence. In 5 of these 10, the residual lesions were exacerbated and 2 required a further operation. The remaining 5 patients showed recurrence at the site of previous anastomosis and 2 of these 5 required additional surgery.In group B, comprising patients with small ulcers, aphthoid ulcers, or scars, and group C, comprising patients with no residual lesions, recurrence was observed in 13 of 16, and 3 of 9 patients, respectively. The recurrent lesions were all found proximal to, or at the site of previous anastomosis. Additional operations were performed on 3 of the group B patients. The findings of this study revealed that recurrence requiring additional surgery is more frequent at the site of anastomosis, regardless of the endoscopic appearance of the residual lesions.     

The epidemiology of Alzheimer disease in intellectual disability: results and recommendations from an international conference

Among adults with intellectual disability, virtually everyone with Down's syndrome (DS) over the age of 40 years has neuropathology currently viewed to be consistent with a diagnosis of Alzheimer disease (AD), while other adults with intellectual disability without DS display an increased prevalence of Alzheimer-type neuropathology after they reach the age of 65. This paper presents the results of discussions by an epidemiology workgroup, formed at an international conference convened to discuss AD among people with intellectual disability, concerning: (I) the incidence and prevalence of clinical dementia in adults with intellectual disability; (2) risk factors for the development of AD in adults with intellectual disability; and (3) a minimum data set that would be of great utility for future research on AD in adults with intellectual disability.     

Lymphocyte predominance Hodgkin's disease (nodular paragranuloma)–a bcl-2 negative germinal centre lymphoma

Hodin's disease, lymphocyte predominance type (nodular paragranuloma), is of germinal centre origin and the tumours cells have a B-cell phenotype. As the t(14;18) translocation, and the subsequent expression of bcl-2 protein by germinal centre cells, is the most characteristic finding of centroblastic-centrocytic lymphoma, we have tested a series of 11 cases of lymphocyte predominance Hodgkin's disease, using Southern blot analysis for the major breakpoint region and the minor breakpoint cluster region, polymerase chain reaction with primers for the major and minor breakpoint cluster region, and immunohistological studies with a monoclonal antibody specific for the bcl-2 protein. All three techniques gave negative results in the cases of Hodgkin's disease, establishing a clear differentiation from centroblastic-centrocytic lymphoma. These findings are useful in the differential diagnosis between the two entities and raise the question of the non-clonal nature of lymphocyte predominance Hodgkin's disease.