High expression of α 2, 3-linked sialic acid residues is associated with the metastatic potential of human gastric cancer

Background: Sialic acid, as a terminal saccharide residue on cell surface glycoconjugates, plays an important role in a variety of biological processes. However, the precise nature of the molecules in gastric cancers has not been unveiled nor documented to be of clinical relevance. Herein, we measured the expression of α 2, 3-linked sialic acid residues by using a specific lectin as well as the potential of invasion and metastasis of gastric cancer was analyzed. Methods: The expression of α 2, 3-linked sialic acid residues in 100 cases of gastric cancer samples was evaluated using Maackia amurensis leukoagglutinin (MAL) histochemical staining analysis. The assays of cytochemical staining and flow cytometry were employed to determine the MAL positive cells in the gastric cancer cell lines. The activities of invasion and migration were evaluated using the assays of cell adhesion and transwell chamber. Results: The staining of MAL in gastric cancer tissues showed that high levels of α 2, 3-linked sialic acid residues were closely associated with the invasive depth (P = 0.0003) and lymph node metastasis (P = 0.0441). In gastric adenocarcinoma cell lines, SGC-7901, the highly metastatic cell line, displayed the most positive reaction with MAL among the selected cell lines. The potential of invasion and migration was confirmed using the assays of adhesion and transwell chamber that SGC-7901 exhibited the high activity of adhesion to extracellular matrix (ECM) and penetration to Matrigel. Conclusion: These results suggested that high level of α 2, 3-linked sialic acid residues was associated with metastatic potential of gastric cancer cells.     

怀槐化学成分的研究(Ⅱ)

目的 :对怀槐心材乙醇提取物的乙酸乙酯萃取部分进行化学成分的研究。方法 :硅胶柱色谱和SephadexLH-20凝胶色谱分离纯化 ;UV ,IR ,MS ,HNMR ,^{13相似文献}   

基于棘的显微结构的海盘车属形态学研究

目 的 研究海盘车属的棘的形态学差别。方 法 借助显微照相和扫描电镜等新的方法,对中国海域海盘车属4种海盘车的棘进行研究。结 果 记录4种海盘车棘的数量排列和形态,同时对不同部位棘进行拍照记录,归纳出基于棘显微结构的检索表。     

多棘海盘车的化学成分研究进展

国内外对多棘海盘车的化学成分进行大量的研究,从中分离得到多种独特的化学成分及新成分,包括海星皂苷、甾体硫酸盐、甾体、氨基酸、多糖等,其中很多化合物具有促进抑菌和溶血等方面的药理活性,查阅和收集国内外相关文献进行归纳、分析和综述,为多棘海盘车的进一步开发研究提供依据。     

怀槐树皮异黄酮苷类化学成分研究

研究怀槐 (Maackia amurensis) 树皮中的化学成分。利用大孔吸附树脂、硅胶、凝胶和ODS柱色谱等分离技术分离化合物, 根据化合物的理化性质和光谱数据鉴定其结构。从其70%乙醇提取物的正丁醇萃取部分分离得到了13个异黄酮苷类化合物, 分别为4′,6-二甲氧基-异黄酮-7-O-β-D-葡萄糖-(1→6)-β-D-吡喃葡糖苷 (1), 樱黄素- 4′-O-β-D-葡萄糖-(1→6)-β-D-吡喃葡糖苷 (2), 芒柄花苷 (3), 黄豆素苷 (4), 染料木苷 (5), saikoisoflavonoside A (6), 阿夫罗摩辛-7-O-β-D-吡喃葡糖苷 (7), 阿夫罗摩辛-7-O-β-D-木糖-(1→6)-β-D-吡喃葡糖苷 (8), 4′-甲氧基-异黄酮- 7-O-β-D-木糖-(1→6)-β-D-吡喃葡糖苷 (9), 4′,6-二甲氧基-异黄酮-7-O-β-D-芹糖-(1→6)-β-D-吡喃葡糖苷 (10), 鸢尾种苷 (11), 5-羟基-4′-甲氧基-异黄酮-7-O-β-D-葡萄糖-(1→6)-β-D-吡喃葡糖苷 (12) 和4′-甲氧基-异黄酮-7-O-β-D-芹糖-(1→6)-β-D-吡喃葡糖苷 (13)。化合物1为新化合物, 命名为怀槐异黄酮苷(maackiaisoflavonoside), 化合物2, 8, 9, 10, 12和13为该属内首次分离得到。     

怀槐异黄酮水解后苷元的含量测定

目的用HPLC法测定怀槐异黄酮水解后苷元芒柄花黄素、阿夫罗摩辛、黄豆黄素的含量。方法经正交试验确定的最佳提取与水解工艺制备供试品溶液,采用高效液相法同时测定水解后3种异黄酮苷元含量。色谱柱:DiamonsilTMC18(250 mm×4.6 mm,5μm),流动相:乙腈-质量分数为0.05%的磷酸水溶液(体积比为35∶65),流速:1.0 mL.min-1,检测波长:260 nm,柱温:35℃。结果芒柄花黄素在5.0~25.2 mg.L-1、阿夫罗摩辛在4.2~21.0 mg.L-1、黄豆黄素在4.8~24.0 mg.L-1时峰面积与质量浓度呈良好的线性关系,相关系数分别为1.000 0、0.999 6、0.999 6。芒柄花黄素、阿夫罗摩辛、黄豆黄素的平均回收率分别为98.43%、97.62%、98.38%,RSD分别为1.04%、0.52%、0.23%。结论HPLC测定方法可用于芒柄花黄素、阿夫罗摩辛、黄豆黄素的含量测定。     

海星Asterias rollestoni的化学成分研究

目的从青岛海星Asterias rollestoni分离并确定结构新颖的活性化合物,并阐明海星中的次生代谢产物的多样性.方法应用正相和反相色谱包括半制备HPLC方法分离和纯化化合物;应用IR,UV,MS,lD和2D NMR,以及理化性质确定化合物的结构.结果从青岛海星Asterias rollestoni中获得17种化学成分,分别为N7-2'-deoxypseudoxanthosine(1),2'-O-methyl-in-osine(2),2'-deoxyinosine(3),2'-O-methylguanosine(4),inosine(5);thymine(6),uracil(7),thymidine(8),deoxyuridine(9),2'-O-methyluridine(10),(-)-(1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(11),(-)-(1R,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(12),(-)-(3S)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(13),L-tryptophan(14),L-phenylalanine(15),3-carboxy indole(16)和p-hydroxybenzoic acid(17).结论其中1为新化合物.它们的化学结构经1D,2D NMR,以及其它波谱分析,结合与已知化合物的波谱和理化数据对照得以确定.化合物8,9,10为首次从天然资源中获得,化合物14和15系首次从该海星中获得.     

海星黄对肾虚大鼠及肾阳虚志愿者的影响

研究海星黄对房劳型大鼠及肾阳虚证志愿者的影响，结果表明，口服给予海星黄2g.d^-1,90d后大鼠组的红细胞数，白细胞数，血红蛋白含量，肾指数，睾丸指数和精囊腺指数均比造型组高，海星黄对肾阳虚志愿者总有效率为90％，所有服用本品者未发现任何不良反应，说明海星黄对大鼠肾阳虚具有显著的滋补作用，对肾阳虚证病人有较好的治疗作用。     

Inhibition of cholinesterase and amyloid-beta aggregation by resveratrol oligomers from Vitis amurensis

In the course of screening for acetylcholinesterase and butyrylcholinesterase inhibitors from natural products by an in vitro Ellman method, the extract of the roots of Vitis amurensis Rupr. (Vitaceae) showed significant cholinesterase inhibitory activity. Employing a bioassay-linked HPLC method, followed by a semi-preparative HPLC method, two compounds of interest were isolated and characterized as vitisin A and heyneanol A. They inhibited effectively both acetylcholinesterase and butyrylcholinesterase in a dose-dependent manner and exhibited higher activity against butyrylcholinesterase compared with that of galantamine, a positive control. Furthermore, the aggregation of beta-amyloid was evaluated in vitro based on a thioflavine T fluorescence assay to expand their activity profile, with the result that both compounds showed the ability to block beta-amyloid aggregation.     

黑水缬草挥发油对中枢神经系统药理作用的研究

目的：观察黑水缬草挥发油对中枢神经系统的作用。方法：考察黑水缬草挥发油对小鼠自主活动的影响、对阈下和催眠剂量戊巴比妥钠的睡眠协同作用、对大鼠睡眠时相的影响、对醋酸所致小鼠扭体反应的影响以及对硫代氨基脲诱发小鼠惊厥的影响。结果：黑水缬草挥发油对小鼠的自主活动具有显著的抑制作用，与戊巴比妥钠有较好的催眠协同作用，可提高小鼠的入睡率，延长小鼠睡眠时间，明显延长大鼠睡眠周期中的慢波睡眠Ⅱ期（SWS2）和快动眼睡眠期（REMS）两个时相，同时能明显减少小鼠扭体反应次数，对抗硫代氨基脲诱发的小鼠惊厥。结论：黑水缬草挥发油具有显著的镇静、镇痛和抗惊厥等中枢神经系统抑制作用，预示了良好的新药开发前景。