Spatial correlation of mouse photoreceptor-RPE thickness between SD-OCT and histology

Spectral Domain Optical Coherence Tomography (SD-OCT) applied to the mouse retina has been limited due to inherent movement artifacts and lack of resolution. Recently, SD-OCT scans from a commercially available imaging system have yielded retinal thickness values comparable to histology. However, these measurements are based on single point analysis of images. Here we report that using the Spectralis HRA + OCT Spectral Domain OCT and Fluorescein Angiography system (Heidelberg Engineering, Heidelberg, Germany), retinal thickness of linear expanses from SD-OCT data can be accurately assessed. This is possible by the development of a Spectralis-compatible ImageJ plug-in that imports 8-bit SLO and 32-bit OCT B-scan images, retaining scale and segmentation data and enabling analysis and 3D reconstruction. Moreover, mouse retinal layer thickness values obtained with this plug-in exhibit a high correlation to thickness measurements from histology of the same retinas. Thus, use of this ImageJ plug-in results in reliable quantification of long retinal expanses from in vivo SD-OCT images.     

Evaluation of the role of spectral-domain optical coherence tomography in the early detection of macular and ganglion cell complex thickness changes in patients with rheumatologic diseases taking hydroxychloroquine

BackgroundThe aim was to investigate the effects of hydroxychloroquine (HCQ) on macular and ganglion cell complex (GCC) thickness measured by spectral-domain optical coherence tomography (SD-OCT).MethodsA total of 145 patients were included in the study. Patients who had taken HCQ for at least 6 months formed group 1 (n:81), while 34 patients with rheumatologic diseases who did not receive HCQ therapy, and 30 healthy subjects formed groups 2 and 3. The patients in group 1 were divided into two subgroups one with more than 60 months of usage (A), and the other with less than 60 months (B). All patients underwent a complete ophthalmologic examination, as well as visual field (VF) testing, and the measurement of macular and GCC thickness with SD-OCT.ResultsThe mean duration of taking HCQ was 100.96±46.97 and 26.50±14.45 months and the total dose was 843.37±489.38 g and 208.63±135.01 g in groups 1A and 1B, respectively. Patients in group 1 had significantly worse VF scores than groups 2 and 3, but no difference was found between groups 1A and 1B. The SD-OCT macular thickness was thinner in the inner and outer nasal quadrants according to the ETDRS 9 sector chart in the patients using hydroxychloroquine, while the GCC in the group 1A patients was thinner in the superior, inferior areas and inferior inner nasal, inferior-superior outer temporal quadrants compared to the other groups (p < 0.05).ConclusionAssessment of regional macular and GCC changes by SD-OCT is a valuable tool for early detection of HCQ maculopathy.     

SD-OCT在观察生理性大视杯人群RNFL及神经节细胞复合体中的应用

目的使用频域OCT（spectral domain optic coherence tomography,SD-OCT）观察生理性大视杯者的视盘形态学参数、视网膜神经纤维层（retinal nerve fiber layer,RNFL）及黄斑区神经节细胞复合体（ganglion cell complex GCC）的表现。方法选取2012年6月-2013年6月50例97眼在眼底检查中发现生理性大视杯者及30例60眼健康人进行SD-OCT检查,测量视盘形态学参数、整体平均RNFL厚度、上方平均RNFL厚度、下方平均RNFL厚度、整体平均GCC的厚度、上方平均GCC厚度、下方平均GCC厚度,并分析大视杯组RNFL与GCC的相关性。结果大视杯组与正常对照组比较,视盘各形态学参数两组之间差异均有统计学意义（P〈0.05）,RNFL及GCC两组间比较差异无统计学意义。整体平均RNFL（P=0.289 1）,上方平均RNFL（P=0.822 3）,下方平均RNF（P=0.140 3）;整体平均GCC（P=0.693 4）,上方平均GCC（P=0.772 4）,下方平均GCC（P=0.6429）;RNFL与GCC在整体（r=0.726 8,P=0.000 0）、上方（r=0.732 0,P=0.000 0）及下方（r=0.666 6,P=0.000 0）的厚度均呈明显的正相关。结论 SD-OCT是一种比较敏感的可观察到视网膜结构改变的检查方法,能够更为全面客观地观察生理性大视杯人群眼底的结构学特点。     

曲安奈德在合并NPDR的白内障手术中的应用

目的：对合并NPDR的白内障患者,在行白内障超声乳化手术中行后筋膜囊下注射TA,通过频域相干光断层深度增强成像(EDI SD-OCT)检查对黄斑区术前术后的分析比较,研究曲安奈德对黄斑水肿的干预及预防作用。

方法：在一项前瞻性随机临床试验中,选取我院合并NPDR的白内障患者,随机分成两组,试验组在实施白内障超声乳化手术时后筋膜囊下注射TA 40mg,对照组仅实施白内障超声乳化吸除手术。术前、术后观察BCVA、IOP、CMT、黄斑中心凹下脉络膜厚度(SFCT)的变化。

结果：两组术前和术后的BCVA均有差异(P<0.05); 术后1wk时,对照组的SFCT比试验组增厚(t=2.165,P<0.05); 术后2wk时,两组间CMT、SFCT均有差异(P<0.05); 术后1mo时,两组间BCVA、CMT均有差异(P<0.05)。两组术前和术后的IOP无差异(P>0.05)。对于术后发生DME,试验组、对照组的发生率分别为0、11%。

结论：NPDR患者在行白内障超声乳化手术中行后筋膜囊下注射TA,可能对DME的发生有积极的预防作用。     

SD-OCT检测Nd:YAG激光周边虹膜切除术后黄斑区视网膜厚度变化

目的：探讨Nd：YAG激光周边虹膜切除术(LPI)对原发性闭角型青光眼(PACG)患者黄斑区视网膜厚度的影响。

方法： 前瞻性研究。选取2019-10/2021-10我院PACG患者30例30眼为PACG组,同期健康体检者20名20眼为正常对照组(随机选取一眼)。PACG组患者接受LPI治疗,术后随访1mo。两组受试者均行最佳矫正视力(BCVA)、眼压、角膜内皮细胞、IOL Master 500光学测量仪测量前房深度(ACD)及眼轴长度(AL)、频域光学相干断层扫描(SD-OCT)检查黄斑区视网膜厚度。比较两组受试者及PACG组患者LPI前后ACD、眼压、AL和黄斑区视网膜厚度,并行相关性分析。

结果：PACG组患者术后1wk,1mo ACD较术前变深(均P<0.05)。PACG组患者手术前后眼压和AL均无差异(均P>0.05)。PACG组患者术前黄斑中心区,内外环上方、颞侧、下方、鼻侧,共9个区域视网膜厚度分别为243.50±13.24、324.50±13.46、308.83±15.94、310.00±14.24、314.50±16.29、300.67±19.95、290.17±12.58、302.40±16.37、307.33±14.84μm,正常对照组相应部位分别为266.14±16.16、342.67±15.86、327.95±16.41、337.85±13.03、341.24±15.58、313.76±17.59、290.24±16.29、303.81±13.91、323.01±14.80μm,两组间中心区、内环上方、颞侧、下方、鼻侧比较均有差异(均P<0.05)。PACG组术后1wk黄斑区9个区域视网膜厚度分别与术前相比均增加,术后1mo接近术前水平,但手术前后各分区比较均无差异(均P>0.05)。

结论：与正常受试者比较,PACG患者黄斑区视网膜厚度较薄。PACG患者LPI术后早期黄斑区视网膜可变厚。     

抗VEGF或抗炎治疗对视网膜静脉阻塞继发不同类型黄斑水肿的短期疗效比较

目的:探讨抗炎治疗及抗血管内皮生长因子(VEGF)治疗对不同种类视网膜静脉阻塞继发黄斑水肿(RVO-ME)患者的效果差异.方法:回顾性收集2019-06/2020-12就诊于郑州大学第一附属医院眼科的RVO-ME患者的临床资料,共计108例108眼,其中黄斑囊样水肿(CME)52例52眼、浆液性神经上皮脱离(SRD)3...