Effects of total glucosides of peony on immunological hepatic fibrosis in rats

AIM: To study the effects of total glucosides of peony (TGP) on immunological hepatic fibrosis induced by human albumin in rats. METHODS: Sixty adult male Sprague-Dawley rats were randomly divided into: Normal group, model group, TGP (60 and 120 mg/kg) treatment groups and colchicines (0.1 mg/kg) treatment group. On the day before the rats were killed, those in TGP or colchicine groups received TGP or colchicine as above from the first day of tail vein injection of human albumin. The rats in normal and model groups were only administered with the same volume of vehicle. At the end of the 16th wk, rats in each group were killed. Blood and tissue specimens were taken. Levels of alanine aminotransferase (ALT), aspartate aminotr-ansferase (AST), nitric oxide (NO), content of malondi-aldehyde (MDA), activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px), were measured by biochemical methods. Serum procollagen type Ⅲ (PC Ⅲ) and laminin (LN) were determined by radioimmunoassay. Liver collagen level was determined by measuring hydroxyproline content in fresh liver samples. Hepatic tissue sections were stained with hematoxylin-eosin and examined under a light microscope. RESULTS: Histological results showed that TGP improved the human albumin-induced alterations in the liver structure, alleviated lobular necrosis and significantly lowered collagen content. The antifibrotic effect of TGP was also confirmed by decreased serum content of LN and PCⅢ in TGP-treated group.Moreover, the treatment with TGP effectively reduced the hydroxyproline content in liver homogenates. However, the level of ALT and AST increased in fibrotic rat but had no significance compared with normal control, whereas the ratio of A/G decreased without significance. TGP had no effect on level of ALT, AST and the ratio of A/G. Furthermore, TGP treatment significantly blocked the increase in MDA and NO, asso- ciated with a partial elevation in liver total antioxidant capacity including SOD and GSH-px. CONCLUSION: TGP has beneficial effects on hepatic fibrosis in rats by inhibition of collagen synthesis and decreasing oxidative stress.     

加味丹参饮预处理对心肌细胞内钙超载的延迟保护作用

目的观察加味丹参饮预处理对心肌细胞内钙超载的延迟保护作用。方法将培养72h的大鼠心肌细胞随机分6组,空白组正常培养;正常血清对照组加50%大鼠血清培养;含药血清组加50%含加味丹参饮的药物血清培养;缺氧再给氧组予缺氧3h,再给氧1h。缺氧预处理组、加味丹参饮预处理组分别给予缺氧预处理、加味丹参饮预处理,24h后再予缺氧3h,给氧1h。结果缺氧再给氧组乳酸脱氢酶(LDH)、肌酸激酶(CK)及细胞内钙离子浓度显著升高(P<0.01),缺氧预处理和加味丹参饮预处理组LDH、CK及细胞内钙离子浓度显著低于缺氧再给氧组(P<0.01),与空白组、血清对照组和含药血清对照组相似(P>0.05)。结论加味丹参饮预处理的延迟保护作用可防止心肌细胞内钙离子超载,从而发挥细胞保护作用。     

润肠口服液的薄层色谱鉴别

目的:采用薄层色谱法(TLC)鉴别润肠口服液中黄芪、白芍、当归.方法:黄芪鉴别以氯仿-醋酸乙酯-甲醇-水(20:40:22:10)10℃以下放置后的下层液为展开剂,以硫酸乙醇溶液(1→10)显色荆;白芍鉴别以氯仿-醋酸乙酯-甲醇-甲酸(40:5:10:0.2)为展开剂,以5%香草醛硫酸溶液为显色剂;当归鉴别以正己烷-醋酸乙酯(9:1)为展开剂.结果:各色谱斑点清晰,分离度好,阴性对照无干扰.结论:该鉴别方法简便、可靠、灵敏、专属性强,可用于润肠口服液的质量控制.     

薄层色谱分析法对赤芍与伪品地榆的比较鉴别

探索有效鉴别出中药赤芍饮片中地榆混伪品的方法。对赤芍和地榆饮片的外观性状和显微特征,用薄层色谱分析法,鉴别赤芍中掺有的地榆混伪品。     

附子汤合芍药甘草汤镇痛抗炎作用研究

目的 :观察附子汤与芍药甘草汤合用的镇痛、抗炎作用。方法 :采用扭体法、热板法和二甲苯致炎法分别检测附子汤、芍药甘草汤及二者合用对小鼠的镇痛率、对痛域提高的百分率和肿胀抑制率。结果 :附子汤与芍约甘草汤合用有镇痛、抗炎作用 ,且强于二方单用。结论 :附子汤与芍药甘草汤合用可使其镇痛、抗炎作用增强。     

赤芍急性毒性实验观察

目的 对发生不良反应的批次赤芍开展调研,探讨其发生不良反应的原因.方法 模拟临床赤芍的用量用法,将赤芍予小白鼠灌胃给药,并设置相应的对照组,观察给药后动物的不良反应情况.结果 在观察的120只样品组小白鼠中,5只小白鼠出现死亡,另有95只出现毛松,肌软,活动减少,呼吸减慢等不良反应.结论 该批次赤芍确实有毒性反应.     

白芍田金龟甲综合防治试验研究

白芍田害虫防治的关键是控制华北大黑鳃金龟的危害。本文就田间防治成虫、白芍栽植前及生长期防治幼虫等问题进行了试验研究。并通过时试验结果的分析,提出了该虫综合防治的技术方案。     

白芍总苷联合抗组胺药治疗慢性荨麻疹疗效和安全性的Meta分析

目的 评价中药白芍总苷联合抗组胺药治疗慢性荨麻疹的有效性和安全性。方法 计算机检索中国知网(CNKI)、维普数据库(VIP)、万方数字化期刊群和PubMed（各数据库检索时间均从创建至2015年9月）,收集相关文献。根据纳入和排除标准筛选并提取资料,采用RevMan 5.2软件进行Meta分析。结果 共纳入19项研究,1606例患者。Meta分析结果显示：白芍总苷可提高抗组胺药治疗慢性荨麻疹的有效率（P<0.00001）,亚组分析结果提示：白芍总苷显著提高咪唑斯汀（P<0.00001）、西替利嗪（P=0.0004）、地氯雷他定（P＜0.00001）、非索非那定（P<0.0001）、依巴斯汀（P<0.002）的疗效;不良反应分析提示,治疗组与对照组差异有统计学意义（P=0.009）。结论 基于现有临床证据,白芍总苷联合抗组胺药治疗慢性荨麻疹的疗效确切,而腹泻发生率明显增加。由于纳入的研究样本数量较少,研究质量不统一,本结论尚需更多大样本、高质量临床随机对照试验予以证实。     

牡丹和芍药种皮、种仁及种皮提取物中10种茋类成分含量及抗氧化测定

常用中药牡丹和芍药种子中含有茋类成分,具有多种药理活性,为了开发和利用牡丹种子资源,该文选择种子中含量相对较高的10种茋类成分suffruticosol A,suffruticosol B,suffruticosol C,trans-resveratrol,cis-ε-viniferin,trans-ε-viniferin,cissuffruticosol D,cis-gnetin H,trans-suffruticosol D和trans-gnetin H为指标进行含量测定。采用HPLC-DAD,YMC-pack ODS-A色谱柱(4.6 mm×250 mm,5μm);流动相以甲醇-水梯度洗脱,流速为1.0 m L·min-1;柱温25℃;检测波长为230 nm。同时采用清除DPPH自由基的方法初步评价其抗氧化作用。结果表明:所建立的方法能够同时测定10种茋类成分的含量;牡丹和芍药种子中的茋类成分主要集中于种皮部位(总质量分数达16.7%以上),种仁中含量很低(总质量分数不到0.3%),种皮提取物富含茋类成分,总含量达75%以上。种皮提取物及10种茋类成分多具有较好的抗氧化潜力。该文对牡丹和芍药资源的综合利用,特别是种皮资源的有效开发和利用奠定了基础。     

A Pharmacological Review of Bioactive Constituents of Paeonia lactiflora Pallas and Paeonia veitchii Lynch

The roots of two peony species, Paeonia lactiflora Pallas and Paeonia veitchii Lynch are routinely referred to as either chishao (赤芍) or baishao (白芍). This paper reviews the botanical origins and traditional medicinal usage of each species, as well as pharmacological like activity of their constituents. A search of herbal pharmacological encyclopaedia, PubChem and PubMed databases identified their known constituents. The biological data for these constituents were evaluated and classified according to pharmacological‐like activity, with emphasis on compounds of greatest concentration and bioavailability. It was found that P. lactiflora and P. veitchii have some common compounds; however, their phytochemical bioavailability varies. Furthermore, a larger number of compounds have been identified in P. lactiflora. These have greater potential for antiinflammatory, antiviral, antibacterial and antioxidant therapeutic activity compared with P. veitchii. However, evidence indicates both species are similarly indicated for antiviral and glycaemic activity. Major compounds of each are classified as flavonoids, hydrolysable tannins (polyphenols) and monoterpene glycosides. The evidence suggests both species, when administered in entire botanical form, have an excellent safety profile; however, constituent toxicity risk evidence is limited, requiring further investigation. Although experiments show many compounds have biological activity, further investigation of their therapeutic potential is needed. Copyright © 2016 John Wiley & Sons, Ltd.