Comparison of the human electro-oculographic response to green and near-ultraviolet stimuli

We recorded the electro-oculogram from 27 normal subjects by means of green and near-ultraviolet (UVA) stimulation. After a 40-minute dark-adaptation period, baseline responses were recorded. In response to the green stimulus, the electro-oculogram increased from this level by an average (± standard error of the mean) of 49.5% ±4.0%. Although the predicted scotopic effectiveness of the ultraviolet stimulus was more than 3 log units below that of the green stimulus, the near-ultraviolet-induced electro-oculogram increased to an average of 21.9% ±3.0% above baseline. This response cannot be due to lens fluorescence to the near-ultraviolet stimulus, since two aphakic subjects had electro-oculographic responses of 32% and 76% above baseline to near-ultraviolet stimuli. Neither the green nor the ultraviolet electro-oculogram changed significantly with age. These large responses to near-ultraviolet stimulation demonstrate the need for standardizing light sources for electro-oculographic testing because the degree of near-ultraviolet irradiance varies considerably according to their design characteristics.     

Radiofrequency Procedures

Abstract:   Radiofrequency is a minimally invasive, target-selective technique that has been in clinical use for more than 25 years and has demonstrated success at reducing pain in several chronic pain conditions, including trigeminal neuralgia, chronic low back pain, postherpetic neuralgia, and complex regional pain syndrome. However, the success of radiofrequency in chronic pain has not been adequately reproduced in good-quality, randomized controlled trials, and its use in the management of neuropathic pain is under some debate. In addition, conventional radiofrequency occasionally leads to worsening and even new onset of neuropathic pain. Nevertheless, clinical experience suggests that radiofrequency may be a useful tool in the overall management of refractory neuropathic pain. Pulsed radiofrequency in particular is a minimally destructive procedure that may offer new opportunities and a broader perspective for therapy with radiofrequency.     

选择性绿激光汽化术治疗良性前列腺增生症

目的探讨选择性绿激光汽化术(PVP)治疗良性前列腺增生(BPH)的方法并评价其疗效。方法采用连续硬膜外麻醉,应用PVP治疗180例BPH患者。使用Laserscope公司生产的绿激光系统,平均功率80W,治疗时间20~140min[(42.5±19.4)min)],能量值10.2~54.2万焦耳[(12.4±5.6)万焦耳],术后留置尿管1~5d。结果术后52例出现一过性的终末血尿,12例暂时性尿失禁,18例短暂性排尿困难,3例迟发性出血。随访3~6个月,术后最大尿流率(Qmax)、IPSS评分、膀胱残余尿量(BRU)均较术前明显改善(P<0.05)。结论PVP治疗BPH操作简单,安全可靠,并发症少,恢复快,是治疗BPH的理想方法。     

强脉冲光对大鼠皮肤胶原蛋白影响的定量分析

目的　定量分析强脉冲光对大鼠皮肤胶原蛋白含量的影响,进一步明确强脉冲光在治疗皮肤光老化中的作用机制。方法　选择15只SD大鼠,每只选3个部位用强脉冲光治疗,采用同一治疗参数,能量密度34J/cm2 ,分为3个脉冲,脉宽各为4ms、5ms、6ms,脉冲延时为2 0ms及2 5ms。分别切取治疗后第1、3、5、7、15、30天皮肤和未治疗部位皮肤,进行组织学观察。结果　治疗后,第1、3、5、7天皮肤胶原蛋白染色与未治疗部位比较无明显差别,第15天强于未治疗部位,第30天明显强于未治疗部位,免疫组化染色Ⅰ、Ⅲ型胶原明显增多(P <0 .0 0 1)。结论　强脉冲光治疗大鼠皮肤后可引起胶原含量的增加,在实验基础上为强脉冲光的临床应用提供了理论依据。     

Dynamics of ablation plume particles generated during excimer laser corneal ablation

Background and Objective: Although the empirical characteristics of ArF excimer laser corneal ablation have been well documented, the exact ablation mechanisms are not well understood. The present paper reports a quantitative analysis of corneal ablation plumes using in situ time resolved laser light scattering and Raman spectroscopy. Study Design/Materials and Methods: Bovine corneas were used as the ArF excimer laser ablation targets. Light scattering data were recorded from the ablation plume as a function of height above the tissue surface and as function of delay time with respect to the ablative ArF laser pulse. Results: Raman spectra of the ablation plume allow identification of the particles as water. Mean plume particle diameters are found to decrease with height, while the particle volume fractions are relatively constant. The total volume of plume particles correlates well with the total volume of water in the ablated corneal tissue. Conclusion: The finding of a non-evolving plume composed of water spherules, combined with the excellent agreement between total volume of water in the plume and the content of water in the ablated corneal tissue, support the concept of photodecomposition or “cold ablation” for corneal tissue during ArF excimer laser ablation. © 1995 Wiley-Liss, Inc.     

轻稀土与联吡啶和菲罗啉三元配合物的合成与表征

合成了稀土（Ｌｎ）与２，２－联吡啶（Ｌ１）、１，１０－菲罗啉（Ｌ２）的三元固态配合物。通过元素分析、ＩＲ谱、ＴＧＡ谱和摩尔电导等对该系列配合物进行了表征，实测结果与通式ＬｎＬ１Ｌ２Ｃｌ３·３Ｈ２Ｏ符合较好。抗菌试验表明，该系列配合物有较强的广谱抗菌作用。     

猪心肌球蛋白及其轻,重链亚基的纯化和鉴定

采用一种改进的方法，从１００ｇ猪心室肌中提取心肌肌球蛋白３７０ｍｇ，并进一步分离和纯化其亚基成分，分别得到肌球蛋白轻链２４ｍｇ和重链２１０ｍｇ，核酸含量＜１％，不含有肌动蛋白、原肌球蛋白、肌钙蛋白及其它杂蛋白；ＳＤＳＰＡＧＥ显示，猪心肌球蛋白重链为２００～２２０ＫＤ一条带，肌球蛋白轻链则为２７ｋｄ、２１．４ｋｄ、１９ｋｄ三条带；纯化猪心肌球蛋白的Ｃａ２＋激活ＡＴＰ酶活性为０．２４μｍｏｌＰｉ／（ｍｇ·ｍｉｎ），但其分离后的亚基则无此活性。     

κ及λ轻链测定在多发性骨髓瘤诊断中的应用

目的：寻求准确，可靠的免疫球蛋白及κ和λ轻链测定方法，提高多发性骨髓瘤分型和鉴别诊断水平。     

Quantitative analysis of the suppressive effect on the light rise of the hypertonic solution

We previously reported the hyperosmolarity response (a decrease of the ocular standing potential by hyperosmolarity) as a new clinical test of the retinal pigment epithelium (RPE) activity. In the present study a hypertonic solution (Fructmanit, 1.4 × 10³ m0sm/1) was intravenously injected for 20 min in proportion to a subject's total blood volume (TBV). At the injection speed of 5, 10, and 15% of the subjects' TBV per hour the mean amplitude of the hyperosmolarity response in normal subjects was 19.7, 30.1 and 36.4% respectively. The amplitude of the hyperosmolarity response depends on the logarithm of the dose of the hypertonic solution within the range of the dose tested.We previously found that hyperosmolarity suppresses the light rise. The present study investigated this suppressive effect in a quantitative manner. The light rise (a full-field illumination of 1.2 × 10³ cdl/m²) was dose-dependently suppressed by Fructmanit. The mean of the light rise to dark trough ratio in normal subjects was 1.81 with no osmotic stress, and 1.64, 1.41 and 1.29 respectively at the injection speeds of 5, 10, and 15%. The suppression of the light rise by hyperosmolarity is compatible with the view that the hyperosmolarity response and the light rise share the basal membrane of the RPE as the main site of their generation.