胎脑组织培养移植治疗大脑发育不全实验与临床研究

２０只同种成年纯白兔，分别用新鲜和培养孕龄１０～１３ｄ的胎兔脑组织、组织培养物，钻孔脑皮质内移植，每处移植脑组织０．３～０．５ｇ，移植物可以在体内存活、生长、增殖。在动物实验的基础上，对６例不同原因引起的大脑发育不全病人采用４个月水囊引产的胎儿脑组织行多点钻孔，多面脑皮质内移植，每例移植胎脑组织８～１２ｇ。结果：５例症状当天即有改善；随访１．５～２ａ，３例癫痫停止发作；４例肌张力明显降低，肢体功能显著改善；智商提高３８～５１分，脑电图慢波减少，癫痫波消失，ＢＡＥＰ、ＶＥＰ、ＳＥＰ、ＭＥＰ不同程度改善；１例临床症状稍有改善，有效率８３％。结果提示：胎脑组织移植治疗大脑发育不全是有效的。     

一氧化氮抑制牵张刺激心肌细胞肥大反应的实验研究

目的　探讨一氧化氮 (NO)对牵张刺激心肌细胞肥大的抑制作用。方法　用分光光度法、原位杂交及图像分析法测定蛋白、DNA含量及 β MHCmRNA表达。 结果　 2 0 %牵张刺激可诱导培养心肌细胞内游离Ca2 + 含量迅速升高 ,12～ 2 4h即见蛋白质合成及 β MHCmRNA显著增加。大于 3× 10 - 4mol L的硝普钠 (NO供体 )可显著抑制牵张刺激诱导的心肌细胞蛋白质合成及 β MHCmRNA表达。NO可同时使牵张刺激引起的细胞内游离Ca2 + 浓度升高受到抑制。结论　一氧化氮可显著抑制牵张刺激诱导的心肌细胞肥大反应 ,这种抑制可能是通过降低细胞内游离Ca2 + 浓度而发挥作用的。     

1-(2,6-二甲基苯氧基)-2-(3，4-二甲氧基苯乙氨基)丙烷盐酸盐对心肌肥厚大鼠左心室N- ras, P53 mRNA表达的影响

为研究1-(2，6-二甲基苯氧基)-2-(3，4-二甲氧基苯乙氨基)丙烷盐酸盐(DDPH)对心肌肥厚的逆转作用，用部分狭窄腹主动脉方法造成大鼠心肌肥厚模型，从术后wk 4开始，ig DDPH 25和50 mg·kg^-1·d^-1，持续8 wk. 术后12 wk，各组大鼠体重无显著性差异，但模型组心重/体重，左心室重/全心重明显高于对照组. 模型组心肌组织N-ras mRNA表达比对照组高，而抑癌基因P53 mRNA表达明显低于对照组，DDPH可逆转上述变化. 表明DDPH可逆转腹主动脉狭窄所致心肌肥厚.     

原发性高血压患者心律失常与左室肥厚关系的研究

为探讨原发性高血压患者心律失常与左室肥厚 (LVH)的关系 ,用 2 4h动态心电图和超声心动图 ,分析 90例高血压病人心律失常与左室肥厚及心肌缺血的关系。结果 :90例高血压病人检出室性心律失常 4 7例 ,占 5 2 .2 % ;房性心律失常 6 1例 ,发生率 6 7.8% ,伴LVH、ST T改变者室性心律失常的发生率明显高于无LVH者 (P <0 .0 1 )和无ST T改变者 (P <0 .0 1 )。提示 :原发性高血压患者心律失常的发生与左室肥厚及心肌缺血有密切关系     

卡维地洛改善SHR左室舒张功能及可能机制

目的:探讨卡维地洛对自发性高血压大鼠心肌细胞肌浆网(SR)膜上钙泵活性的影响及可能机制.方法:将12周龄雄性SHR40只随机分成4组.第1组在未作治疗时即测定各指标,并与同周龄wistar(n=10)比较,作为治疗前对照.另3组经生理盐水、倍他洛克(Bet)、卡维地洛(Car)治疗20周后,观察各项指标之变化.结果:与wistar比,SHR的平均动脉压、左室肥厚指数、左室舒张末压及心肌细胞SR膜上钙泵活性明显降低.经治疗后,Bet与Car有相同的降血压及减慢心率作用,但Car降低左室舒张末压、左室肥厚指数及提高心肌细胞SR膜上钙泵活性作用比Bet强.结论:Car能显著提高心肌细胞SR膜上钙泵活性,降低左室舒张末压.这与其全面阻滞β1β2α1后逆转左室肥厚、减低外周血管阻力后减少了心肌耗氧量有关.     

Effects and mechanism of different adrenergic receptor antagonists on left ventricular hypertrophy subsequent to coarctation of abdominal aorta in rats

Objective: To study the changes of a collagen-binding protein (Colligin) and myosin heavy chain isoform (α/β-MHC) gene and protein in left ventricular hypertrophy subsequent to coarctation of abdominal aorta in rats and the effects of three kinds of adrenergic receptor blockers: Carvedilol (CAR) , Metoprolol (MET) and Terazosin (TER) on these changes, and to elucidate the effects and new mechanism of CAR on left ventricular hypertrophy regression. Methods: A model of hypertrophy induced by coarctation of abdominal aorta(CAA)was used in this study. Thirty two male wistar rats were divided randomly into four groups 4 weeks after CAA operation: CAA, CAR, MET and TER. Hemodynamics, ventricular remodeling parameters, expressions of Colligin and α/β-MHC mRNA, protein expressions of Collagen Ⅰ / Ⅲ and Colligin were investigated in the four groups and sham operation group. Results: Left ventricle hypertrophy was observed clearly 16 weeks after operation. The ratio of α/β-MHC mRNA decreased, while express     

Primary hypertrophic pyloric stenosis in the adult

A case is reported of a 55 year old male patient with primary hypertrophic pyloric stenosis who was subjected to distal gastrectomy. Adult hypertrophic pyloric stenosis is an uncommon condition which is usually misdiagnosed as carcinoma of the antrum. It is a benign disease resulting from hypertrophy of the circular fibres of the pyloric canal and is recognizable radiologically by narrowing and elongation of the pyloric canal and endoscopically by appearances resembling those of the cervix. This condition is probably congenital although aetiology has not been established. In the absence of symptoms, no clinical treatment is required. However, surgical intervention is advocated, when stenosis gives rise to symptoms, there is a suspicion of malignancy, or the ulceration due to the disease. Distal gastrectomy with gastroduodenostomy is the treatment of choice.     

老年高血压病左室肥厚QT离散度改变及缬沙坦的影响

目的探讨老年高血压病左室肥厚(LVH)QT离散度(QTd改变及缬沙坦对其影响.方法对86例老年高血压病患者和31例年龄相当的健康人对照组进行超声心动图、体表心电图检查,并对LVH组行6 mon缬沙坦降压治疗后复查超声心动图和心电图,计算QTd及LVH.结果①高血压伴LVH组(n=33)QTd明显大于正常对照组(n=31)和高血压无左室肥厚组(n=53).②经缬沙坦治疗后LVH逆转、QTd明显缩短.结论高血压左室肥厚的QT离散度增加,持续缬沙坦治疗能逆转LVH并改善QTd.     

Madigan式前列腺摘除术41例报告

采用Ｍａｄｉｇａｎ手术治疗良性前列腺增生４１例，结果手术均顺利，获得了满意的疗效．结果表明，由于该术式不切开膀胱，保留了尿道的完整，因而缩短了留置尿管时间，减少了住院天数，大大降低了术后并发症如尿失禁、尿道狭窄和逆行射精的发生率，疗效满意．     

The response to alpha blockade in benign prostatic hyperplasia is related to the percent area density of prostate smooth muscle.

The objective of the present study was to determine whether the smooth muscle content of the prostate adenoma is related to the clinical response to terazosin, a long-acting selective alpha 1 blocker. Multiple random biopsies of the prostate were obtained from 26 male subjects with symptomatic benign prostatic hyperplasia (BPH) prior to initiating therapy with terazosin. Double immunoenzymatic staining and computer-assisted quantitative color image analysis were utilized to quantify the area density of smooth muscle, connective tissue, glandular epithelium, and glandular lumen. The clinical response to alpha blockade was based upon changes in peak urinary flow rate and the Boyarsky symptom score. A significant direct relationship was observed between the percent area density of smooth muscle and the percent change in peak urinary flow rate. A statistically significant correlation between the percent area density of smooth muscle and the percent change in Boyarsky symptom score was not observed. The percent area density of prostate smooth muscle in the subjects exhibiting a favorable clinical response was 38% greater than the nonresponders (P = 0.068). The clinical response to alpha blockade in BPH is related to the area density of prostate smooth muscle.