当归芍药散合槐花散加减治疗溃疡性结肠炎活动期大肠湿热证的临床观察

目的:观察当归芍药散合槐花散加减治疗溃疡性结肠炎(UC)活动期大肠湿热证及对脑肠肽神经递质和炎细胞因子的影响。方法:共纳入130例随机按数字表法分成对照组和观察组各65例。对照组口服美沙拉嗪肠溶片1. 0 g/次,3次/d;重度患者给予醋酸泼尼松片,0. 75 mg·kg~(-1)·d~(-1),分次口服。观察组在对照组治疗的基础上给予当归芍药散合槐花散加减内服,1剂/d。两组疗程均为连续治疗4周。进行治疗前后症状评分,Mayo评分、结肠黏膜评分和炎症性肠病问卷(IBDQ)评分;对于进入缓解期的患者进行6个月的随访,记录复发情况;检测治疗前后血管活性肠肽(VIP),P物质(SP),生长抑素(SS),白细胞介素-1(IL-1),IL-6,IL-4和IL-10。结果:经4周的治疗,观察组临床缓解率为93. 22%,优于对照组的80. 7%(χ~2=4. 035,P 0. 05);观察组患者各症状评分均低于对照组(P 0. 01);观察组患者Mayo评分和结肠黏膜评分均低于对照组(P 0. 01);观察组患者IBDQ量表各个维度评分和IBDQ总分均高于对照组(P 0. 01);对临床缓解患者进行6个月随访,观察组复发率为18. 18%,低于对照组的36. 96%(χ2=4. 509,P 0. 05),且复发时间长于对照组(P 0. 01);观察组患者血清IL-1,IL-6水平均低于对照组(P 0. 01),IL-4,IL-10水平均高于对照组(P 0. 01);观察组患者血浆VIP和SS均高于对照组(P 0. 01),SP水平低于对照组(P 0. 01)。结论:在西医常规治疗的基础上,当归芍药散合槐花散加减治疗UC(大肠湿热证)活动期患者,近期可控制疾病活动,促进结肠黏膜修复,远期可推迟复发,降低复发率,并可调节脑肠肽神经递质和促炎/抑炎细胞因子水平。     

三仁汤对湿热证型病毒性肺炎模型小鼠免疫功能的干预作用

目的:探讨三仁汤对湿热证型病毒性肺炎模型小鼠免疫功能的干预作用。方法:将55只小鼠随机分为空白组、模型组、三仁汤组、利巴韦林组,其中空白组10只,其余3组每组15只。除空白组外其他3组通过肥甘饮食、湿热环境、流感病毒感染三因素合并,构建湿热证型病毒性肺炎动物模型。滴鼻感染H1N1病毒2 h后灌胃给药,每天2次,连续给药6 d后处死动物,观察并记录小鼠肛温、体质量;苏木精-伊红染色法(HE)观察小鼠肺组织病理变化;酶联免疫吸附法检测血清中白细胞介素-1(IL-1)、白细胞介素-4(IL-4)、干扰素-γ(IFN-γ)浓度;流式细胞仪法检测全血中CD3+、CD3+/CD4+、CD3+/CD8+含量。结果:与模型组相比,三仁汤组小鼠血清中IFN-γ、IL-1水平升高,IL-4水平降低(P0.05);外周血中CD3+、CD3+/CD4+恢复良好,达空白组水平(P 0.05),CD3+/CD8+含量有升高(P0.05)。结论:三仁汤具有改变机体炎性因子含量、增强机体免疫功能的作用。     

基于肺与大肠相表里理论针刺治疗内痔湿热下注证的临床疗效观察

目的：基于肺与大肠相表里理论针刺治疗内痔湿热下注证的疗效观察。方法：将符合纳入标准的 264 例患者随机设计分为两组，治疗组和对照组各 132 例，治疗组为基本穴加肺与大肠相关取穴及辨证取穴联合口服地奥司明药物治疗，对照组则去除肺经相关取穴，两组均以 14 d 为一疗程。观察记录针刺治疗后病人的便血、痔核充血等情况。结果：治疗组总有效率为 91.5%，明显高于对照组 82.3%（P<0.05）。与治疗前相比，两组便血、痔核脱出、肛门不适、肛门疼痛等临床体征及症状均有明显改善（P<0.05）；治疗后，治疗组对临床体征及症状的改善明显优于对照组，差异有统计学意义（P<0.05）。治疗结束 2 周、1 月后，治疗组的复发率明显低于对照组（P<0.05）。结论：基于肺与大肠相表里理论针刺治疗内痔湿热下注证的疗效满意，值得临床推广应用。     

特异性皮炎的辨证论治

特异性皮炎多发于儿童,其特征为瘙痒剧烈,反复发作,并常有家族史。中医认为与先天禀赋有关,涉及脾、心脏腑,治疗以清热利湿止痒,清心培土,祛风润燥为主。     

Effect of adhesive properties of buffy coat on the quality of blood components produced with Top & Top and Top & Bottom bags

BackgroundThe Transfusion Medicine Unit of Reggio Emilia currently collects whole blood using conventional quadruple Fresenius Top & Top bags. In this study, new Fresenius Top & Bottom bags were assessed and compared to the routine method with regards to product quality and operational requirements.ResultsCompared to the traditional system, the whole blood units processed with Top & Bottom bags yielded larger plasma volumes (+5.7%) and a similar amount of concentrated red blood cells, but with a much lower contamination of lymphocytes (−61.5%) and platelets (−86.6%). Consequently, the pooled platelets contained less plasma (−26.3%) and were significantly richer in platelets (+17.9%).DiscussionThis study investigated the effect of centrifugation on the adhesiveness of the buffy coat to the bag used for whole blood collection. We analysed the mechanism by which this undesirable phenomenon affects the quality of packed red blood cells in two types of bags. We also documented the incomparability of measurements on platelet concentrates performed with different principles of cell counting: this vexing problem has important implications for biomedical research and for the establishment of universal product standards. Our results support the conclusion that the Top & Bottom bags produce components of higher quality than our usual system, while having equal operational efficiency. Use of the new bags could result in an important quality improvement in blood components manufacturing.     

中医药调节免疫治疗慢性肾炎湿热证

中医认为,慢性肾炎为一组本虚标实的证候,湿热证是其最常见的邪实见证.慢性肾炎与免疫介导的炎症反应有关,细胞因子(CK)在肾小球炎症发病过程中发挥重要作用,与肾炎的发生、发展及预后紧密相关.中医药从调节免疫治疗肾炎湿热证,即从细胞因子水平研究中医药作用机制,确定湿热证细胞因子水平客观辨证指标,为肾炎湿热证辨证治疗提供了新依据.     

Online volume rendering of incrementally accumulated LSCEM images for superficial oral cancer detection

Laser scanning confocal endomicroscope (LSCEM) has emerged as an imaging modality which provides non-invasive, in vivo imaging of biological tissue on a microscopic scale. Scientific visualizations for LSCEM datasets captured by current imaging systems require these datasets to be fully acquired and brought to a separate rendering machine. To extend the features and capabilities of this modality, we propose a system which is capable of performing realtime visualization of LSCEM datasets. Using field-programmable gate arrays, our system performs three tasks in parallel: (1) automated control of dataset acquisition; (2) imaging-rendering system synchronization; and (3) realtime volume rendering of dynamic datasets. Through fusion of LSCEM imaging and volume rendering processes, acquired datasets can be visualized in realtime to provide an immediate perception of the image quality and biological conditions of the subject, further assisting in realtime cancer diagnosis. Subsequently, the imaging procedure can be improved for more accurate diagnosis and reduce the need for repeating the process due to unsatisfactory datasets.     

崔公让治疗早期带状疱疹经验

带状疱疹是一种呈带状分布、痛如火燎的急性疱疹性皮肤病。本病的重要特点是疼痛剧烈,病程迁延。崔公让教授临床上采用中药内服外用兼食疗进行治疗,在缓解早期带状疱疹性疼痛及预防后遗神经痛方面取得了明显的疗效。     

Effect of yishen huayu fang on kidney tissue E-cadherin expression in unilateral ureter ligation in rats

[OBJECTIVE]:To observe E-calcium sticky protein (E-cadherin) expression in kidney tissues in a rat model of unilateral ureter ligation and the effect of Yishen Huayu Fang (formula oftonifying the kidney and dissolving accumulated blood stasis) on the expression.[METHODS]:A total of 150 clean grade male rats were randomly divided into a control group,model group,low-dose Yishen Huayu Fang group (low-dose group),high-dose Yishen Huayu Fang group (high-dose group),and Lotensin group.A renal fibrosis model was established with unilateral ureteral obstruction (UUO).Pathological changes of rat renal tissue were observed with light microscopy on days 3,7,14,21,and 28 after UUO.Changes in kidney tissue E-cadherin expression were observed with immunohistochemistry.[RESULTS]:Three days after modeling,kidney edema appeared followed by gradual inflammatory cell infiltration,and part of the small tubules disappeared while the renal cortex thinned.Meanwhile,the E-cadherin expression level dropped,which was negatively correlated with the obstruction time.After intervention,E-cadherin expression was increased in all treatment groups (P＜0.01 or P＜0.05),while there were no significant differences between the high-dose and Lotensin groups.[CONCLUSION]:Yishen Huayu Fang delays the renal fibrosis process by promoting E-cadherin expression in renal tissues and reducing extracellular matrix deposition.     

中医体质与子宫肌瘤的关系探讨

目的：探讨中医体质与子宫肌瘤发病的关系。方法：以中医基础理论为指导,结合王琦教授的体质学说,分析体质与子宫肌瘤发病的内在关系。结果：30～50岁的女性中痰湿质、湿热质、血瘀质、气郁质是子宫肌瘤的易发人群,尤其是在春季及长夏易发病。结论：在子宫肌瘤的好发阶段,可以有针对性地指导患者通过运用药物、加强锻炼等途径来调整病理体质从而有效减少并预防子宫肌瘤的发生与发展。