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51.
目的:比较苏云金芽孢杆菌Bti-951发泡块剂(由本所研制)和Bti-Ft发泡块剂(德国提供)灭白纹伊蚊效果。方法:模拟现场试验和现场试验。结果:室内生物测定,Bti-951发泡块剂LD50为0.2365μg/ml;Bti-Ft发泡块剂LD50为0.0903μg/ml。在3.4~3.7m2现场水池中,两种发泡块剂使用剂量为0.5、1.0、1.5g/m2,制剂24小时灭蚊效果均达100%;当剂量为1.5g/m2时,Bti-951发泡块剂持效15天,Bti-Ft可达20天。结论:该剂型运用于小型水体,便于贮存、携带和使用,比乳剂、粉剂持效更长。 相似文献
52.
健康志愿者10名,随机交叉口服硫酸吗啡控释片(CRMS)30mg(30mg×1)和硫酸吗啡普通片(IRMS)20mg(10mg×2),分别于服药前后各时点取静脉血,用GCMS测定血浆中吗啡含量。以药代软件程序处理,分别求得CRMS和IRMS的Cmax为19.38±3.80和21.27±6.21ng/ml;tmax为2.36±0.37h和0.55±0.16h;t1/2β为3.53±0.87h和3.03±0.74h,曲线下面积AUC为145.15±17.65和93.08±16.65ng·h/ml。癌症病人多次口服硫酸吗啡至稳态,CRMS和IRMS的峰浓度分别为27.43±0.33ng/ml,22.68±0.16ng/ml;谷浓度分别为19.45±1.44ng/ml;18.14±0.49ng/ml。 相似文献
53.
Carl Andrew Castro John B. Hogan Kimberly A. Benson Christina W. Shehata Michael R. Landauer 《Pharmacology, biochemistry, and behavior》1995,50(4):521-526
Experimental drugs and compounds that do not easily dissolve in water or saline are frequently combined with vehicles like solvents, detergents, or vegetable oils. Yet very little has been reported on the behavioral effects of vehicles. In this study, we assessed the effects of a vegetable oil (emulphor-620), two detergents (Tween-20 and Tween-80), and two solvents [dimethyl sulphoxide (DMSO) and ethanol] on the locomotor activity in CD2F1 male mice. Locomotor activity was monitored for 12 h after vehicle administration (IP). The concentrations for each vehicle were expressed as percent of vehicle in saline (v/v). Emulphor-620 did not affect locomotor activity at any concentration tested (2%, 4%, 8%, 16%, and 32%). Tween-20 significantly decreased locomotor activity at a concentration of 16% and Tween-80 at 32%. DMSO significantly decreased locomotor activity at concentrations of 32% and 64%. In contrast, ethanol produced a biphasic behavioral response: increased activity at a concentration of 16% and decreased activity at a concentration of 32%. These results will facilitate the selection and concentration of vehicles to be used in combination with experimental drugs or test agents. 相似文献
54.
Koichi Nishimura Takashi Hajiro Toru Oga Mitsuhiro Tsukino Susumu Sato Akihiko Ikeda 《The Journal of asthma》2005,41(2):141-146
Simple and concise measures for health status are desirable in clinical practice. The Asthma Bother Profile (ABP), which consists of 23 items, has been developed to assess how much asthma bothers patients. The Airways Questionnaire 20 (AQ20) is a simple instrument which consists of 20 items. The purpose of this study was to investigate how the ABP and AQ20 evaluate the health status of patients with asthma. A total of 166 patients with chronic asthma (age: 48 ± 16 yr, 77 males) completed pulmonary function testing, measurement of airway hyperresponsiveness, dyspnea rating, assessments of their anxiety and depression (HADS; Hospital Anxiety and Depression Scale), and assessments of their health status. The health status was assessed using the ABP, AQ20, the short-form 36 health survey questionnaire (SF-36), the Living With Asthma Questionnaire (LWAQ) and the Asthma Quality of Life Questionnaire (AQLQ). The Japanese version of the ABP included only 15 'bother' items out of the original 23 items due to cultural differences. The scores on the ABP were widely distributed, whereas the scores on the AQ20 were skewed towards the milder end of the scale. The ABP had a strong correlation with the Avoidance and Distress constructs on the LWAQ, and Anxiety and Depression on the HADS (Rs = 0.56 ∼ 0.79), and its strongest correlation with the General Health (Rs = - 0.64) scale among the 8 subscales on the SF-36. The AQ20 had a less significant correlation with the LWAQ, AQLQ, and SF-36 than the ABP. The ABP and AQ20 were short and simple to complete, and both measures could easily be used in clinical practice. The ABP can evaluate patients more specifically with respect to distress and bother than the AQ20. 相似文献
55.
K. Hatayama J. Kambayashi T. Kawasaki K. Morimoto T. Ohshiro T. Mori 《Thrombosis research》1986,41(6):761-770
The interrelationship between ATP-secretion, protein phosphorylation and intracellular Ca2+ concentration ([Ca2+]i) was studied in both 32P and quin 2 loaded human platelets stimulated by thrombin or thromboxane A2 analogue (STA2). In platelets stimulated by thrombin, the degree of 47,000 dalton polypeptides (P47) phosphorylation was observed in completely dose-related manner, regardless of the amount of [Ca2+]i. In the same condition, the degree of myosin light chain (P20) phosphorylation, however, was well correlated with ATP secretion and [Ca2+]i, when platelets were stimulated by lower dose of thrombin. The similar results were obtained in platelets stimulated by STA2. These findings suggested that P20, but not P47, phosphorylation in activated platelets is mediated by a rise of [Ca2+]i and is well correlated with the secretory reaction. It was unlikely that P47 phosphorylation plays any role in promoting platelet activation. 相似文献
56.
57.
嵌合抗CD20抗体片段F(ab'')2的表达及活性 总被引:1,自引:0,他引:1
目的 :为了简化生产步骤 ,提高抗体蛋白的生物活性 ,探索在工程菌体内直接进行抗CD2 0F(ab’) 2 的高效率分泌性表达。方法 :采用单因素考察法优化培养条件 ,使蛋白G柱和S2 0 0 HR分子筛柱分离纯化目的蛋白 ,用MTT法检测抗CD2 0F(ab’) 2 抑制Daudi细胞体外生长的活性。结果 :发现用论文所选定的最适培养条件 ,抗CD2 0F(ab’) 2 的产量有了明显提高 ,从1 9~ 2 .2mg L达到了 3 7~ 4 3mg L ,F(ab’) 2 在表达产物中所占的比例也从 9 7%~ 13 2 %提高到了 38 1%~ 4 6 8% ;使用S2 0 0 HR分子筛柱对蛋白G柱亲和纯化后的产物进一步分离纯化 ,可以使F(ab’) 2 的纯度达到 85 %以上 ;MTT法检测结果证明F(ab’) 2 抑制Daudi细胞生长的IC50 值为 14 6 μg ml,而Fab’为 39 5 μg ml。 结论 :实现了抗CD2 0F(ab’) 2 在工程菌体内的高效率分泌性表达 ,而且所表达的抗CD2 0F(ab’) 2 比抗CD2 0Fab’具有更强的抑制Daudi细胞体外生长的能力 相似文献
58.
目的 了解初中新生慢性鼻-鼻窦炎发病与鼻中隔偏曲的相关性。 方法 分层随机抽取郑州市1910名12~15岁初中新生,采取问卷调查及专科体检的形式,根据量表、体检结果,了解鼻中隔偏曲和慢性鼻-鼻窦炎患病情况,分析两者相关性。 结果 抽样初中新生慢性鼻-鼻窦炎的患病率为6.2%(119/1910);慢性鼻-鼻窦炎患病群体与非患病群体中,轻、中度鼻中隔偏曲的发生率分别为27.7%(33/119)和31.2%(559/1791),两者差异无统计学意义(χ2=0.632,P>0.05);重度鼻中隔偏曲的发生率分别为13.4%(16/119)和8.0%(144/1791),两者差异有统计学意义(χ2=4.248,P<0.05)。 结论 初中新生慢性鼻-鼻窦炎发病与重度鼻中隔偏曲具有相关性。 相似文献
59.
Werner M Mattis A Aubele M Cummings M Zitzelsberger H Hutzler P Höfler H 《Virchows Archiv : an international journal of pathology》1999,435(5):469-472
The 20q13 region harboring recently described putative oncogenes is frequently amplified in invasive ductal carcinoma (IDC).
The aim of this study was to examine the 20q13 copy number in intraduct hyperplasia (IH), atypical duct hyperplasia (ADH),
and ductal carcinoma in situ (DCIS) adjacent to IDC. In 5 patients, comparative genomic hybridization (CGH) after laser microdissection
revealed 20q13 amplification in four of five cases of IH, in all of three cases of IH with atypia, all five of DCIS, and all
five of IDC. Fluorescence in situ hybridization (FISH) confirmed the amplification at 20q13.2 in IH in the two specimens analyzed.
The amplification rate, however, was higher in DCIS and IDC. In phenotypically normal ductal epithelium normal values were
found for 20q13 copy number by FISH (n=2) and CGH (n=5). Although the number of cases presented here is small, our results suggest that mutations in the 20q13.2 region in IH
may be associated with accelerated proliferation and hyperplasia of the ductal epithelium. Progression to DCIS and ICD is
accompanied by a further increase in the 20q13.2 copy number.
Received: 17 March 1999 / Accepted: 22 June 1999 相似文献