细胞间粘附分子—1反义DNA体外对内皮细胞细胞间粘附分子—1？…

目的：研究细胞间粘附分子－１（ＩＣＡＭ－１）的 ＤＮＡ对内皮细胞ＩＣＡＭ－１表达的抑制作用。方法：构建ＩＣＡＭ－１反义ＤＮＡ载体,用脂质体转染内皮细胞,ＴＮＦα刺激后,流式细胞术检测转染细胞表面ＩＣＡＭ－１蛋白的表达。结果：酶切鉴定证明,１．４ｋｂ的ＩＣＡＭ－１ＤＮＡ片段后向连接到ｐｃＤＮＡ３表达载体,;流式细胞术检测显示,正常细胞加ＴＮＦα刺激后,表面ＩＣＡＭ－１表达明显升高,转洒细胞加ＴＮＦα     

The modulation of attachment,growth and morphology of cancerous prostate cells by polyelectrolyte nanofilms

The behaviour of cancerous epithelial prostatic cells (PC3) growing on polyelectrolytes (PE) coatings was compared to the behaviour of immortalized normal prostatic cells (PNT-2). The cell behaviour was evaluated and quantified in terms of initial cell attachment, growth, metabolic activity, morphometry, adhesion, apoptosis and stress related gene expression. Both the anionic PSS (poly(sodium 4-styrenesulphonate))-terminated surface and cationic PAH (poly(allylamine hydrochloride))-terminated surfaces were not cytotoxic. The initial attachment of cells was better on the PAH-terminated surface compared to fibronectin. However, the proliferation rate of PC3 cells was reduced on the PAH-terminated surface and slightly increased on the PSS coatings. Only PAH prevented the clustering phenotype of PC3 and reduced the number of focal adhesion points as compared to fibronectin or PSS coatings. In contrast, none of the PE surfaces significantly affected the biological responses of PNT-2 cells. PAH-terminating films provide a tool to preferentially modulate the growth of some cancerous phenotypes, in this case as a micro-environment that reduces the growth of metastatic PC3 cells.     

ChIP-chip analysis of neurexins and other candidate genes for addiction and neuropsychiatric disorders

Abstract:

Several addiction susceptibility genes have been mapped by linkage and genomewide association. However, functional alleles associated with disease risk have not been identified, with a few possible exceptions. In addition, little is known about the cis- and trans-acting factors involved in regulating their expression. To address these issues, we used a ChIP-chip approach to identify regulatory elements in fetal-brain– targeting genes implicated in addiction and other neuropsychiatric conditions. Our data point to a number of putative regulatory elements, several of which, we show, are functionally significant. Many established or putative regulatory elements map near-disease–associated SNPs. These regions would be of interest to survey for patient-specific functional variants involved in disease susceptibility.     

Effect of Yiqitongyanghuatan Recipe on Experimental Atherosclerosis in Rabbits

Cardiac and cerebrovasculur disease is one of themost serious disease threatening human health and lifecurrently. Atherosclerosis is the main pathologicalbasis of ischemic heart disease and cerebrovasculardisease. So prevention of atherosclerosis is alsoprimary strategy for heart and cerebrovasculurdiseases. The etiology of atherosclerosis is blood stasisand phlegm retention in traditional Chinese medicine.However,blood stasis and stagnation of phlegm haveequivalent position in clinical treat…     

人工关节假体不同材料及材料表面粗糙度对表皮葡萄球菌粘附能力的影响

目的探讨人工关节假体材料及表面粗糙度对表皮葡萄球菌粘附能力的影响。方法制作超高分子聚乙烯、钛合金和钴铬钼合金试样，表皮葡萄球菌经FITC标记，人工关节材料试样消毒后接种FITC标记的表皮葡萄球菌，试样表面分为光滑表面和粗糙表面，每组各6个试样，将含有细菌和试样的24孔板在37℃下孵育30min后，用荧光显微镜观察，试样用扫描电镜观察。结果对于光滑的人工关节常用材料表面，表皮葡萄球菌对超高分子聚乙烯（UHMWPE）的粘附能力显著高于钛合金和钴铬钼合金（P〈0．001），对钴铬钼合金的粘附能力要高于钛合金（P〈0．05）；粗糙的超高分子聚乙烯和钴铬钼合金表面比光滑的表面更易引起表皮葡萄球菌的粘附（P〈0．01），而细菌对粗糙钛合金的粘附仅轻微高于光滑钛合金（P〉0．05）。荧光照相观察及扫描电镜观察显示细菌在粗糙表面的划痕内聚集粘附。结论本研究结果表明细菌对人工关节材料表面的粘附能力不但取决于细菌本身，也和材料性质和表面粗糙度有关。     

Modified implant surfaces show different biofilm compositions under in vivo conditions

Objective: Plaque accumulation on implant surfaces can result in peri-implantitis with potential implant loss. The aim of the present study was to examine the influence of zirconium nitride (ZrN) as a potential implant surface on the biofilm composition and diversity in vivo .
Material and methods: ZrN- or titanium (Ti)-coated glass specimens and ZrN or roughened Ti discs were used as substrates. Pure glass and polished titanium served as controls. The specimens were mounted on removable intraoral splints in five adults. After 24 h of intraoral exposure, the biofilms were analyzed applying single-strand conformation polymorphism (SSCP analysis) of 16S rRNA genes. Sequence analysis of the dominant bands excised from the SSCP fingerprints allowed to taxonomically describe bacteria derived from biofilm samples.
Results: The highest number of bands was counted on pure glass and Ti 800. ZrN-coated glass and ZrN-coated titanium discs showed the lowest values for species richness. However, no significant differences were observed regarding the diversity of the identified bacterial species among all the surfaces examined. A total of 46 different bacteria were identified. The dominant bands within the fingerprints indicated bacteria belonging to the Streptococcus group as identified by their 16S rDNA sequence.
Conclusion: A coating of glass surfaces with ZrN significantly reduced the species richness in early bacterial colonization but the diversity was not significantly changed. In consideration of the results obtained by this and former studies a ZrN coating appears to rather modify the quantity of early bacterial adherence than the quality of the microbial community structure.     

川芎嗪对妊娠高血压综合征患者脐静脉内皮细胞粘附分子表 …

目的 探讨妊娠高血压综合征（妊高征）患者血浆诱导培养的脐静脉内皮细胞（ＨＵＶＥＣ）表面细胞间粘附分子－１（ＩＣＡＭ－１）、血管细胞粘附分子－１（ＶＣＡＭ－１）的表达以及川芎嗪对其的影响。方法 采用胶原酶、胰蛋白酶混合灌注消化法，对正常妊娠妇女（正常妊娠组）、妊高征患者（妊高征组）的ＨＵＶＥＣ进行培养，待细胞融合成单层后，加或不加川芎嗪进行预处理，并以正常未妊娠妇女（对照组）作为对照。再加入上述３组     

细胞粘附分子与肝脏移植排斥反应

同种移植排斥至今仍是器官移植后一个尚未完全解决的难题，也是移植失败的主要原因，。近年来，许多学者研究证实：细胞粘附分子在肝脏同种移植排斥反应中起到至关重要的作用。综述了细胞粘附分子的生物学特点，在肝脏移植排斥反应中的诱导和表达及其在诊断和治疗中的研究进展。     

nm23、CD44H和CD44V6蛋白在胃癌的表达及意义

目的 研究胃癌组织中ｎｍ２３和ＣＤ４４的表达，以及与淋巴结转移和预后的关系。方法应用ＳＰ免疫组化技术对１０５例胃癌组织ｎｍ２３、ＣＤ４４Ｖ６的表达进行观察，并对其中５９例患者随访。结果 ｎｍ２３、ＣＤ４４Ｈ及ＣＤ４４Ｖ６蛋白表达阳性率分别为４４．８％、５４．３％和４８．６％；ｎｍ２３及ＣＤ４４Ｖ６在胃癌组织的表达与其分化、浸润深度、临床分期和预后有关，而ＣＤ４４Ｈ的表达与上述临床病理指标无关。３种