三伏贴中芥子碱及细辛挥发油促进HaCaT细胞摄取延胡索乙素的作用及其机制研究

目的研究三伏贴中芥子碱硫氰酸盐(SPT)及细辛挥发油(AEO)促进角质形成细胞HaCaT摄取延胡索乙素(THP)的作用及其机制。方法采用MTT法检测SPT、AEO和THP对HaCaT细胞活力的影响;根据方中THP自发荧光的特点,采用激光共聚焦扫描显微镜可视化观察SPT及AEO促进HaCaT细胞摄取THP的作用,并用HPLC法测定HaCaT细胞中THP的量;以1,6-二苯基-1,3,5-己三烯(DPH)为荧光探针,利用荧光偏振技术研究SPT、AEO及THP对HaCaT细胞的细胞膜流动性的影响。结果 SPT及AEO均能显著促进THP被HaCaT细胞摄取,且AEO的促进作用优于SPT。SPT、THP、AEO分别作用于DPH标记的HaCaT细胞后,AEO组HaCaT细胞的荧光偏振度和细胞膜微黏度均显著降低,细胞流动性增加,证明AEO是通过提高细胞膜流动性从而促进HaCaT细胞摄取THP,而THP、SPT均不能增加细胞膜流动性。结论三伏贴中SPT及AEO均可促进HaCaT细胞摄取THP,AEO促进机制与其提高细胞膜流动性相关,而SPT的促进作用不是通过增加细胞膜流动性实现,其具体机制有待进一步探明。     

江浙沪名老中医临证运用膏方经验解构研究

目的：基于中医本体特色,引入文本挖掘方法,结合原始病案数据进行集内关联挖掘,形成医案解析,从中分析江浙沪名老中医使用膏方的经验。方法：采用Medcase 数据处理平台,对江浙沪名老中医运用膏方的医案采用计量性趋势数据解构研究。结果：本次研究涉及病例472诊次,涉及病机250条,治法400条,药物529种。结论：江浙沪名老中医运用膏方针对临床常见虚损性疾病,临床核心高频适应症以乏力、寐差、腰酸、纳差等为主;相关高频舌脉为苔薄、白,质红、淡,脉细、弦;核心高频病机为肝肾亏虚、脾肾两虚;高频用药为黄芪、白术、茯苓、当归、芍药、甘草、熟地等药物,组方以四君子汤、四物汤、八珍汤、当归补血汤、六味地黄丸、生脉饮为基础方剂进行复法配伍,辅料常用阿胶、冰糖、龟板胶、鹿角胶等材料,治法多采用气血并调,阴阳并补,虚实并治等复法组合干预亚健康状态。     

Electrode reactions and accumulation of hydrogen at carbon paste electrodes in the presence of tetrachloropalladate

The electrochemical behaviour of the unmodified carbon paste electrode (CPE) in the presence of [PdCl₄]^2? in chloride solutions of different pH (in the range from 3 to 7) is studied by cyclic voltammetry (CV). During cathodic treatment or cycling, Pd(0) is deposited in situ on the electrode surface. Results obtained at negative potentials as a function of the concentrations of Pd(II) and protons in the solution are presented. It is concluded that hydrogen ions are reduced in different ways: first, the reduction at deposited Pd(0) or the simultaneous reduction of H⁺ and [PdCl₄]^2?, respectively, which occurs during the whole cathodic cycle; second, the reduction in the potential range from about ?0.5 to ?1.0 V versus SCE, where surface groups are reduced at the unmodified CPE. The second pathway is the prevailing reduction at lower pH, which occurs at the carbon surface probably via protonated chloropalladate complexes adsorbed at the electrode surface. Potentiostatic pretreatment in the voltammetric range between 0 and +0.2 V leads in solutions of pH 5 to a pronounced increase of the H⁺ reduction current caused by an increase of the sorption capacity of the electrode or by the accumulation of protons at the electrode surface. Protons could be accumulated at the electrode surface by formation of protonated chloropalladate complexes at the CPE.     

中医膏方在分化型甲状腺癌术后心悸中的应用

[目的]总结笔者运用膏方辨治分化型甲状腺癌术后心悸的临床经验。[方法] 通过对分化型甲状腺癌术后出现心悸的病因病机进行分析,总结笔者运用膏方辨治此类心悸的观点、临床经验及独到之处,并通过案例具体说明。[结果] 笔者认为分化型甲状腺癌术后心悸的病机为术后脏腑失养、阴阳气血亏虚,复因药物扰心、情志内伤。病性虚实兼杂,虚处常见气血不足、阴阳失衡、心神失养,实者为药毒、血瘀、痰饮。临证时应根据心率,辨清虚实病机。制膏时注意选择道地药材,注重辅料。所举验案一属阳气亏虚、痰湿郁滞,拟温阳化浊膏治疗;验案二属气阴两虚、痰瘀互结,治以益气养阴、化痰行瘀,拟养阴通络膏治疗。同时,两案均配以原剂量左甲状腺素钠片,终获良效。[结论]治疗分化型甲状腺癌术后心悸患者,在三因制宜的基础上,注重中西理念融合,参考患者病机、临床用药特点,自拟膏方用于临床,增效防复。膏方药效稳定,患者依从性更高,具有较强的临床推广价值。     

Comparison of Metallization Schemes on Glass Dielectrics for X-Band Glass Antennas and Energy Harvesting

We prepare and test four types of glass antennas for X-band applications and energy harvesting. These antennas are made of three different glass metallization schemes, including conductive copper foil (CCF), conductive silver paste (CSP) and indium tin oxide (ITO) thin film. Compared with conventional microstrip patch antennas, the dielectric substrate materials of these designs are replaced with silicon-boron glass (ε_r = 6, tangent δ = 0.002). The antenna with CCF as a radiator and ground plane (case one) is compared with the antenna with ITO replacing the radiator (case two) and ground plane (case three), respectively, and the glass antenna made of CSP (case four) is also presented. In this paper, these four types of glass antennas are measured and analyzed, and a comparison of the fabrication process and performance of these antennas is demonstrated. This study could contribute to the development of human-machine interactivity (HMI) systems with glass dielectric substrates.     

罗美沙星碳糊电极的制作及应用

目的　建立新药盐酸罗美沙星主药含量的快速分析方法。方法　以盐酸罗美沙星与硝酸铋配合比为 1∶1的配位化合物作为电活性物质 ,研制了罗美沙星碳糊电极。结果　此电极得到了良好的电极响应曲线 ,响应斜率为 5 8.0 5mV/pC ,线性范围达 4个数量级 ,检出下限为 1.0× 10 -5mol·L-1。十多种常见无机离子与一些吡啶类离子对电极基本无干扰。结论　可采用直接电位法测定市售罗美沙星片的主药含量。     

Trace iron determination in aminoisophthalic acid using differential-pulse cathodic stripping voltammetry at carbon paste electrodes

Application of differential-pulse cathodic stripping voltammetry using a carbon paste electrode (consisting of carbon powder and liquid paraffin) have been investigated for trace determination of iron in 5-aminoisophthalic acid (AIPA). Samples were dissolved in 1 M HCl, pH was adjusted to 4–5 after addition of EDTA. Voltammetric measurements were performed after filtration. No sample decomposition (mineralization) was necessary. The method showed a good linearity between current and concentration from 3×10⁻⁷ to 5×10⁻⁵ mol dm⁻³ of iron, with a detection limit of 3×10⁻⁷ mol dm⁻³ (resp. 1 ppm in solid AIPA). The results agreed well to those obtained by atomic absorption spectrometry (AAS) using electrothermic atomisation. For AAS measurement, however, microwave digestion of samples was necessary.     

Cortisomol根管糊剂根尖封闭能力的实验研究

目的:探讨Cortisomol根管封闭剂的根尖封闭能力。方法:选取44颗离体单根管恒牙,随机分为2组,每组20颗牙,分别用Cortisomol糊剂和氧化锌丁香油糊剂加牙胶尖以冷侧压法充填根管,采用染料渗入法测量染料渗入距离。结果:Cortisomol糊剂组与氧化锌丁香油糊剂组根尖微渗漏均值分别为(1.21±0.47)mm和(2.17±1.08)mm,经t检验,P<0.01,两组渗漏值具有显著性差异。结论:Cortisomol根管糊剂对根尖的封闭能力优于传统的氧化锌丁香油糊剂。     

In Situ Remediation of Phosphogypsum with Water-Washing Pre-Treatment Using Cemented Paste Backfill: Rheology Behavior and Damage Evolution

The accumulation of original phosphogypsum (OPG) has occupied considerable land resources, which have induced significant environmental problems worldwide. The OPG-based cemented paste backfill (OCPB) has been introduced as a promising solution. In this study, a water-washing pre-treatment was used to purify OPG, aiming to optimize the transport performance and mechanical properties of backfills. The overall results proved that in treated phosphogypsum-based cemented paste backfill (TCPB), the altered particle size distribution can alleviate the shear-thinning characteristic. The mechanical properties were significantly optimized, of which a maximum increase of 183% of stress value was observed. With more pronounced AE signals, the TCPB samples demonstrated better residual structures after the ultimate strength values but with more unstable cracks with high amplitude generated during loading. Principal component analysis confirmed the adverse effects of fluorine and phosphorus on the damage fractal dimensions. The most voluminous hydration products observed were amorphous CSH and ettringite. The interlocked stellate clusters may be associated with the residual structure and the after-peak AE events evident in TCPB, indicate that more significant stress should be applied to break the closely interlocked stitches. Ultimately, the essential findings in this experimental work can provide a scientific reference for efficient OPG recycling.     

Systematic Compounding of Ceramic Pastes in Stereolithographic Additive Manufacturing

In this paper, stereolithographic additive manufacturing of ceramic dental crowns is discussed and reviewed. The accuracy of parts in ceramic processing were optimized through smart computer-aided design, manufacturing, and evaluation. Then, viscous acrylic resin, including alumina particles, were successfully compounded. The closed packing of alumina particles in acrylic pastes was virtually simulated using the distinct element method. Multimodal distributions of particle diameters were systematically optimized at an 80% volume fraction, and an ultraviolet laser beam was scanned sterically. Fine spots were continuously joined by photochemical polymerization. The optical intensity distributions from focal spots were spatially simulated using the ray tracing method. Consequently, the lithographic conditions of the curing depths and dimensional tolerances were experimentally measured and effectively improved, where solid objects were freely processed by layer stacking and interlayer bonding. The composite precursors were dewaxed and sintered along effective heat treatment patterns. The results show that linear shrinkages were reduced as the particle volume fractions were increased. Anisotropic deformations in the horizontal and vertical directions were recursively resolved along numerical feedback for graphical design. Accordingly, dense microstructures without microcracks or pores were obtained. The mechanical properties were measured as practical levels for dental applications.