六例神经型布鲁菌病患者临床特点分析

目的探讨神经型布鲁菌病的临床特点,以提高对该病的诊治能力。方法对运城市中心医院感染科自2014年1月至2019年12月收治的6例确诊为神经型布鲁菌病的患者,收集并分析其流行病学史、临床表现、辅助检查结果、治疗经过、预后等临床资料。结果6例患者均为男性,2例患者有明确的流行病学史,1例患者合并睾丸炎,1例患者伴脑血管病变相关影像学异常;3例患者脑脊液培养出马耳他布鲁菌,1例患者血培养出马耳他布鲁菌;2例患者脑脊液虎红平板凝集试验阳性,脑脊液二代测序结果为布鲁菌属核酸序列;予抗生素治疗后6例患者均预后良好。结论神经型布鲁菌病的临床表现多样,脑脊液培养、脑脊液布鲁菌凝集试验、脑脊液二代测序等多种方法联合应用可以提高神经型布鲁菌病的诊断率。     

Ocular surface involvement in patients with rheumatoid arthritis: Relation with disease activity and duration

Aim of the workThe aim of the present work was to present the frequency of ocular surface manifestations in rheumatoid arthritis (RA) patients and to determine its correlation with disease activity and duration.Patients and methodsThis cross-sectional study included 70 RA patients. Disease activity score (DAS-28) was scored. All patients underwent complete ophthalmic evaluation including assessment of visual acuity. Ocular surface assessment by Rose Bengal (RB) stain, and Schirmer test (ST) was carried out.ResultsSeventy patients (137 eyes) were studied; 3 eyes were excluded due to previous eye surgery. Patients were 67 women and 3 men with a mean age of 47.96 ± 10.8 years, and disease duration 9.19 ± 6.26 years. The mean DAS28 was 4.1 ± 0.77. Rheumatoid factor was positive in 39 (55.7%). Mean best-corrected visual acuity was 0.47 ± 0.33, ST was 10.6 ± 6.1 mm/5 min and RB score was 6.8 ± 3.6. 70% had ocular manifestations in the form of dry eye (54.7%), pterygium (16.1%), posterior blepharitis (5.8%), scleritis (1.3%), corneal thinning (13.9%), filamentary keratitis (8.7%) and maculopathy (11.7%). There was a significant correlation between ST and disease duration (r = −0.17, p = 0.04). Morning stiffness had a significant correlation with both ST (r = −0.2, p = 0.02) and RB (r = 0.22, p = 0.01) and remained significant on regression analysis. DAS-28 was not significantly associated with any of the ocular surface abnormalities.ConclusionOcular manifestations are significant in patients with RA. The most common ocular manifestation is dry eye. Ocular manifestations are significantly associated with the disease duration and should be expected regardless of disease activity in RA patients.     

Combination of in ovo electroporation and time‐lapse imaging to study migrational events in chicken embryos

Background : During embryonic development cell migration plays a principal role in several processes. In past decades, many studies were performed to investigate migrational events, occurring during embryonic organogenesis, neurogenesis, gliogenesis or myogenesis, just to name a few. Although different common techniques are already used for this purpose, one of their major limitations is the static character. However, cell migration is a sophisticated and highly dynamic process, wherefore new appropriate technologies are required to investigate this event in all its complexity. Results and Conclusions: Here we report a novel approach for dynamic analysis of cell migration within embryonic tissue. We combine the modern transfection method of in ovo electroporation with the use of tissue slice culture and state‐of‐the‐art imaging techniques, such as confocal laser scanning microscopy or spinning disc confocal microscopy, and thus, develop a method to study live the migration of myogenic precursors in chicken embryos. The conditions and parameters used in this study allow long‐term imaging for up to 24 hr. Our protocol can be easily adapted for investigations of a variety of other migrational events and provides a novel conception for dynamic analysis of migration during embryonic development. Developmental Dynamics 243:690–698, 2014. © 2013 Wiley Periodicals, Inc.     

Recognizing the CT Manifestations of Gynecologic Conditions Encountered in the Emergency Department

Women commonly present to the emergency room with subacute or acute symptoms of gynecologic origin. Although a pelvic exam and ultrasound (US) are the preferred initial diagnostic tools for gynecologic entities, a CT is often the first line imaging modality in the emergency department. We will provide a review of normal uterine enhancement and normal pregnancy related findings, and then familiarize radiologists with the CT appearances of gynecologic entities classically described on ultrasound that may present to the emergency department.     

Recognition Theory in Nurse/Patient Relationships: The contribution of Gillian Rose

Recognition theory attempts to conceptualize interpersonal relationships and their normative political implications. British social philosopher Gillian Rose developed her own version of recognition rooted in the work of Georg Hegel. This article applies Rose's theory of recognition to care, arguing that its emphasis on lack of identity, the dynamic process of recognition and the existential risks involved accurately describes the relationship between nurse and patient. Rose's version is compared to both contemporary notions of the interpersonal in healthcare literature, other forms of recognition theory and Emmanuel Levinas's vision of intersubjective relationships, a traditional philosophical basis for nursing care. In each case, I will argue that Rosean recognition is a fuller, more complex and ultimately more satisfying view of the relationships involved.     

金樱子醇提颗粒处方工艺研究

目的：筛选金樱子醇提取物颗粒剂的制备工艺.方法：通过综合评分法确定颗粒剂的最佳辅料比,设计L9（34）正交试验,以粒度为评价指标确立处方工艺,并在此基础上考察了临界相对湿度.结果：该颗粒剂采用湿法制粒,最佳处方组成为稠膏-乳糖-可溶性淀粉-10％淀粉浆（5∶12∶3∶0.6）.结论：该颗粒成型性好,相对临界湿度符合生产要求.     

玫瑰花鞣质提取物体外抗氧化的活性研究

目的：研究玫瑰花鞣质提取物体外抗氧化活性的能力。方法：采用Fenton体系和邻苯三酚自氧化法评价了玫瑰花鞣质的体外抗氧化能力,采用多元线性回归对鞣质浓度与抗氧化活性相关性进行了分析。结果：玫瑰花鞣质提取物浓度在0.05~4.00μg/mL范围内与对—OH和O2-清除能力呈量效关系,其最大清除率分别为62.85%和72.67%;浓度对抗氧化能力的线性回归方程为Y=-0.517＋1.732X1-0.903X2。结论：玫瑰花鞣质具有较强的抗氧化能力,是1种天然有效的抗氧化剂。