止淋合剂治疗急性下尿路感染临床研究

目的:观察止淋合剂对急性下尿路感染的疗效。方法:随机分为两组,治疗组服用止淋合剂,对照组服用分清止淋丸,连续服药1周。对用药前后两组症状、尿常规检查、总疗效进行比较。结果:止淋合剂组治疗急性下尿路感染的疗效明显高于分清止淋丸组(P<0.05)。结论:止淋合剂治疗急性下尿路感染具有较好的疗效。     

Specific inhibition of viral neuraminidases by an inhibitor, neuraminin, produced by Streptomyces sp.

Neuraminin was characterized with respect to its inhibition of viral and bacterial neuraminidases. It inhibits the viral but not the bacterial enzymes. In addition, neuraminin distinguishes influenza from Newcastle disease virus. Neuraminin inhibits hydrolysis of fetuin, orosomucoid, and human red blood cell surface glycoproteins by influenza virus or its isolated neuraminidase. The inhibition does not occur when neuraminlactose or colominic acid (a homopolymer of N-acetylneuraminic acid) is used as the substrate. In contrast, neuraminin equally effectively inhibits the action of the Newcastle disease virus toward these substrates. The inhibition was noncompetitive and saturated at a very low concentration of the inhibitor irrespective of the kind and concentration of the substrates.     

著名中医学家林兰教授学术经验之十二 起病隐匿 易漏诊误诊 辨证施治 宜标本兼顾——治疗糖尿病周围神经病变经验

糖尿病周围神经病变属于中医学血痹、痿证等范围。为糖尿病日久 ,气滞血瘀 ,经脉闭阻 ,血管供血不足所致。本病起病隐匿 ,极易漏诊误诊 ,辨证宜分清标本缓急 ,灵活选方用药。     

脾胃肾虚生痰湿祛痰利湿重健脾——治疗糖尿病高脂血症的经验

糖尿病高脂血症属于中医学“痰证”、“湿证”范畴。病因与饮食、内伤、外感、体质等多因素有关 ,病机为脾胃、肾虚 ,湿浊内停 ,蕴久成痰 ,治疗以健脾益肾 ,利湿祛痰为法 ,灵活处方用药     

MicroRNA let-7与胃癌关系的研究进展

胃癌仍然是目前全球癌症的第三大死因,因此早期发现和成功治疗胃癌是目前临床关注的问题。let-7家族是秀丽隐杆线虫中发现的第二个Micro RNA。最近的研究发现let-7在胃癌中表达降低,循环let-7是胃癌的生物标志,因此进一步确定let-7的基因靶点及信号通路是目前研究的热点。现综述Micro RNA let-7在胃癌诊断治疗中的研究进展。     

Utility of multifrequency bioelectrical impedance compared with dual-energy x-ray absorptiometry for assessment of total and regional body composition varies between men and women

Multifrequency bioelectrical impedance analysis of body composition may be an appropriate alternative to dual-energy x-ray absorptiometry. We hypothesized that there would be no significant differences between dual-energy x-ray absorptiometry and either the Biospace (Los Angeles, CA, USA) InBody 520 or 720 multifrequency bioelectrical impedance analysis devices for total lean body mass (LBM), appendicular lean mass (ALM), trunk lean mass (TM), and total fat mass (FM) in 25 men and 25 women (including lean, healthy, and obese individuals according to body mass index), age 18 to 49 years, weight of 73.6 ± 15.4 kg. Both devices overestimated LBM in women (~ 2.5 kg, P < .001) and underestimated ALM in men (~ 3.0 kg, P < .05) and women (~ 1.0 kg, P < .05). The 720 overestimated FM in men (1.6 kg, P < .05) and underestimated TM in women (0.6 kg, P ≤ .05). Regression analyses in men revealed R² (0.87-0.91), standard error of the estimate (SEE; 2.3-2.8 kg), and limits of agreement (LOAs; 4.5-5.7 kg) for LBM; R² (0.62-0.87), SEE (1.5-2.6 kg), and LOA (3.2-6.0 kg) for ALM; R² (0.52-0.71), SEE (2.4-3.0 kg), and LOA (4.6-6.1 kg) for TM; and R² (0.87-0.93), SEE (1.9-2.6 kg), and LOA (5.9-6.2 kg) for FM. Regression analyses in women revealed R² (0.87-0.88), SEE (1.8-1.9 kg), and LOA (4.1-4.2 kg) for LBM; R² (0.78-0.79), SEE (1.4-1.5 kg), and LOA (2.7-2.9 kg) for ALM; R² (0.76-0.77), SEE (1.0 kg), and LOA (2.2-2.3 kg) for TM; and R² (0.95), SEE (2.2 kg), and LOA (4.3-4.4 kg) for FM. The InBody 520 and 720 are valid estimators of LBM and FM in men and of LBM, ALM, and FM in women; the 720 and 520 are valid estimators of TM in men and women, respectively.     

沉默Lin28b基因表达促进人结肠癌细胞奥沙利铂化疗敏感性

目的 探讨人结肠癌细胞中Lin28b基因表达及其与奥沙利铂化疗敏感性之间的关系.方法 构建干扰Lin28b的shRNA质粒(sh-Lin28b),并转染至结肠癌细胞(Cac02、SW480和HCT116细胞);逆转录-聚合酶链反应(RT-PCR)和Western blot法分别检测结肠癌细胞中Lin28b在mRNA和蛋白水平的表达;用细胞计数试剂盒(CCK-8)检测sh-Lin28b和奥沙利铂化疗协同作用,Transwell实验检测处理后的结肠癌细胞迁移能力.结果 RT-PCR和Western blot结果证实Cac02、HCT116及SW480结肠癌细胞中均有Lin28b基因表达,其中在SW480细胞中的蛋白表达量比在HCT116中高1.83倍.SW480和HCT116细胞中Lin28b的mRNA表达在50 nmol/L的sh-Lin28b作用下分别被下调36.4％和90.2％,在100 nmol/L的sh-Lin28b的条件下分别被下调61.8％和89.5％.CCK-8结果显示奥沙利铂处理48 h后,HCT116细胞的IC50值为(6.09±1.42) mg/L,SW480细胞升高34.3％,至(8.18 ±3.64) mg/L.sh-Lin28b联合奥沙利铂处理组比较于对照转染组(NC)抑制SW480和HCTll6细胞生存率分别为(55.10±0.78)％和(50.30±0.69)％.Transwell 实验结果显示SW480细胞的迁移能力在sh-Lin28b的作用下降低至(48.60±0.92)％.结论 沉默表达于结肠癌细胞的Lin28b,可显著抑制结肠癌细胞的迁移能力,并促进奥沙利铂的化疗敏感性.     

林洪生治未病思想在中医药防治肿瘤疾病上的应用

治未病思想最早见于《黄帝内经》,是中医学术思想的重要组成部分,其着重强调防患于未然。林洪生教授将治未病这一重要思想引入到中西医结合防治肿瘤疾病中,具体将其划分为未病先防、既病防变、瘥后防复三个层次,同时运用中医药分阶段规范化治疗的思想配合西医对疾病进行治疗,在临床上取得了显著疗效。文章概括介绍了林洪生教授运用治未病思想指导中西医结合防治肿瘤的理论方法,为其学术思想的传承与推广提供一定参考。