Role of EDRF in the Regulation of Shear Rate in Large Coronary Arteries in Conscious Dogs

To determine whether dilation of large coronary arteries normalizes shear during increased flow following brief occlusion, six dogs were instrumented to measure aortic and left ventricular pressures, left circumflex coronary artery external diameter, and coronary blood flow. The coronary artery was occluded for 15 or 30 s. Data were obtained before and after blockade of EDRF synthesis with nitro-L-arginine. Internal coronary artery diameter and wall shear were calculated on a moment-to-moment basis and the area under the flow curve was measured. Peak flow and shear rate were unaffected by NLA or by the occlusion duration. Flow curve area increased with the duration of occlusion. Internal and external diameters increased significantly for 15 s occlusions before NLA (by 4 ± 1% in external diameter and by 11 ± 4% in internal diameter) and for 30 s occlusions before NLA (by 5 ± 1% in external diameter and by 14 ± 5% in internal diameter) but not after NLA. Adenosine infusions of 0.05, 0.10, 0.50, and 1.0 μmol/kg/min were also used to dilate the coronary arteries. With each infusion, flow, shear and diameter were allowed to reach steady state. Steady state shear was reduced only slightly and did not approach the baseline state. We conclude that increased shear rate causes an increase in coronary artery diameter which is EDRF dependent. Increased coronary artery diameter during reactive hyperemia and adenosine infusions did not normalize wall shear.     

神经生长因子几丁质管修复兔面神经缺损

目的：评价神经生长因子(nerve growth factor，NGF)几丁质管在修复兔面神经缺损中的作用。方法：在16只新西兰兔的两侧面神经上颊支上分别造成8mm缺损，左侧用管腔内注入NGF的几丁质管修复，右侧用自体神经移植修复作对照。术后8周和16周分别取8只动物进行电生理和组织学检查及计算机图像分析。结果：术后8周，实验组的再生神经已通过近远中吻合口，神经纤维密集成束；术后16周，再生神经纤维的数量增加，神经纤维束增粗，形态接近于正常神经。电生理检测和图像分析显示实验组和对照组的神经传导速度和有髓神经轴突总截面积均无显著差异(P＞0．05)。结论：NGF几丁质管可为免面神经缺损提供良好的修复环境。     

蚌埠市某高中学生亚健康状况及其影响因素分析

目的了解蚌埠市高中学生的亚健康状况,探讨其影响因素,为开展相关健康教育工作提供参考依据。方法整群选取蚌埠市某高中在校学生1409名,采用自编问卷进行调查,所得数据采用SPSS10.0进行统计分析。结果亚健康状况检出率为58.34%,不同性别学生亚健康状况检出率差异无统计学意义,不同年级学生亚健康状况检出率差异有统计学意义（P〈0.01）。亚健康的主要危险因素为经常熬夜、正在减肥、缺乏体育锻炼、学习压力大、学习成绩不理想、睡眠不足等12项。结论蚌埠市高中学生亚健康问题较为突出。应积极采取有效措施,提高学生的健康水平。     

高中生自我和谐与应对方式及其相互关系

目的了解高中生自我和谐、应对方式的特征及其相互关系,为开展心理健康教育提供依据。方法采用自我和谐量表及简易应对方式问卷,对755名高中生进行问卷调查。结果高中生总分和各因子分在性别、是否独生子女、城乡、文理科等方面差异均无统计学意义,自我和谐总分存在年级差异;积极应对和消极应对维度在性别、是否独生子女、城乡、文理科等方面差异均无统计学意义,消极应对维度存在年级差异。应对方式与自我和谐总分及分量表得分存在不同程度的相关。结论采用积极应对方式有助于促进高中生的自我和谐,而消极应对方式对自我和谐存在不利影响。     

肺癌患者血清肿瘤特异生长因子检测及临床意义

目的 :研究TSGF在肺癌诊断、疗效判定中的临床应用价值。方法 :利用生化比色法测定了 18例肺良性病变患者 ,2 4例肺癌患者及 9例肺癌患者治疗后血清中TSGF水平。结果 :肺癌患者未经治疗前其血清TS GF水平及阳性率明显高于肺良性病变患者 ,其敏感性为 75 .0 % ,特异性为 77.8%。经有效化学治疗后肺癌患者血清TSGF明显下降。结论 :血清TSGF检测对肺癌的诊断、疗效观察有较重要的意义。     

Factor analysis of bowel symptoms in US and Italian populations

BACKGROUND AND AIMS: Functional gastrointestinal disorders are diagnosed by the presence of a characteristic set of symptoms. Aims of this study were to validate the Rome symptom criteria by factor analysis and to determine whether symptoms cluster in the same way in different cultures. METHODS: One thousand forty-one gastroenterology clinic patients in the US (response rate 53%) and 228 family members accompanying clinic patients in Italy (84%) completed a previously validated symptom questionnaire. Factor analysis identified clusters of symptoms which are highly correlated with each other, and these were compared to the Rome diagnostic criteria. RESULTS: In the US, 13 factors were identified. The irritable bowel factor was composed of three core symptoms corresponding to the Rome II classification system. Two dyspepsia factors were identified which correspond to the ulcer- and motility-like subtypes proposed in the Rome I classification system. All symptoms of constipation formed a single cluster as proposed in the Rome II classification system. Symptom clusters in the US agreed well with symptom clusters identified in Italian subjects. CONCLUSIONS: Empirically derived symptom clusters agree in most respects with the Rome II classification system and support their validity. These symptom clusters are independent of cultural differences in diet and behaviour.     

The surfaces of the parasitic nematodes Trichinella spiralis and Toxocara canis differ in the binding of post-C3 components of human complement by the alternative pathway

The binding of human complement components C3, C5 and C9 to the surface of the infective larvae of the nematode parasites Toxocara canis and Trichinella spiralis, by the alternative pathway, was examined by direct and indirect immunofluorescence on the intact parasites. This showed that although C3 bound to both nematodes, they differed markedly in the binding of C5 and C9; C5 bound only minimally to T. spiralis, and C9 binding to this parasite was barely detectable. In contrast, both early and late components bound to T. canis to a high density, comparable to, or in excess of, the binding of these components to the infective larvae of the trematode Schistosoma mansoni. The lack of binding of the post-C3 components to T. spiralis did not correlate with enhanced binding of the control protein, Factor H.     

凋亡诱导因子在实验性脑卒中后高温状态下的表达

目的探讨caspase非依赖途径在卒中后高温的神经元损伤环节中是否发挥重要作用。方法应用SD大鼠局灶性脑缺血模型,分别设立缺血后高温、缺血后正常温度和假手术组,每组8只。观察动物行为学评分、梗死体积和凋亡诱导因子(AIF)在海马的表达。利用PC12细胞培养缺氧及高温处理后,观察荧光双染AIF在细胞内的表达变化,及流式细胞仪技术检测细胞凋亡水平。结果缺血后高温组的行为学评分和梗死体积明显增加,AIF表达增高。PC12缺氧及高温培养后AIF从30min开始在胞核内表达,且随时间延长逐渐增多,细胞凋亡率随时间延长和温度增高而增加,且不被caspase广谱抑制剂所抑制。结论AIF在卒中后高温的过程中发生由胞浆到胞核的转移,caspase非依赖途径由此发挥重要作用。     

人胰岛素样生长因子-1在大肠杆菌中的高效表达

目的为了获得基因工程重组人胰岛素样生长因子1。方法采用Trizol试剂法，从人胎肝中提取，6-RNA，RT-PCR扩增得到人胰岛素样生长因子1基因，用EcoRⅠ和Hind Ⅲ双酶酶切后，将该基因片段插入表迭栽体pkk223—3，筛选得到了重组质粒pkk-hIGF1。结果加IPTG诱导该质粒转化的大肠杆菌JM109表达，hIGF1的表达水平约30mg／L。结论用肝素亲和层析的方法，获得了电泳纯基因工程重组人胰岛素样生长因子1样品。纯化样品的理化及生物活性分析与天然对照品完全一致。     

主成份分析与因子分析用于多指标综合评价

本文从应用角度阐明了主成份分析与因子分析用于多指标综合评价的异同点，并用实例加以说明。提出了主成份分析和因子分析用于多指标综合评价时，应在能明确主成份或因子的意义和方向情况下加权，否则会得到错误的结果。