Efficacy of catalpol as protectant against oxidative stress and mitochondrial dysfunction on rotenone-induced toxicity in mice brain

Rotenone, a specific inhibitor of mitochondrial complex I, reproduces many features of Parkinson's disease. The aim of the study was carried out to investigate how rotenone affected the mitochondrial function and antioxidant/oxidant parameters of mouse striatum, and secondly, to evaluate the ameliorating effects of catalpol against rotenone-induced damage. Our results showed that rotenone induced significant changes in mitochondrial function such as complex I activity and mitochondrial membrane potential decreased, and enhanced antioxidant status as glutathione depletion, enzymatic (glutathione peroxidase and superoxide dismutase) disorders, and increased lipid peroxidation. Catalpol increased complex I, superoxide dismutase and glutathione peroxidase activities, reduced lipid peroxidation and loss of mitochondrial membrane potential in rotenone-treated mice. These in vivo data indicated that catalpol might have protection against deleterious mouse damage caused by rotenone.     

中药(复方)成分提取动力学数学模型建立及粒径对六味地黄汤中梓醇浓度的影响

目的:建立封闭体系中药(复方)成分溶出动力学数学模型,并对六味地黄汤中有效成分梓醇的提取动力学参数进行研究与分析。方法:将数学建模方法应用于中药提取过程的研究中,根据提取原理、Fick定律建立中药提取过程的数学模型,并对数学模型进行拟合和参数分析,可求得函数表达式。再运用该模型研究六味地黄汤中梓醇的动力学参数。结果:建立了包含三项e的指数形式的成分溶出浓度解析及各参数分析方法。提取六味地黄汤的适宜药材粒度为60目,此时梓醇的最大达峰时间(tmax)为195.1min,达峰浓度(Cmax)为0.01223mg·mL-1,最大提取效率(P)为72.95%,损失率(D)为7.62%。结论:封闭可溶中药(复方)扩散体系的成分溶出符合线性动力学数学模型,各参数可根据溶出浓度表达式关系计算得到。     

均匀设计考察壳聚精澄清法精制降糖通脉水提液

目的:利用均匀设计法探讨壳聚糖澄清剂用于精制降糖通脉水提液时对梓醇含量的影响。方法:用高效液相色谱法对壳聚糖澄清法及乙醇醇沉后的药液所含的梓醇含量进行测定。结果:壳聚糖澄清法可较好地保存降糖通脉君药地黄中有效成分梓醇的含量。结论:壳聚糖澄清法可替代醇沉用于精制降糖通脉水提液。     

高效液相色谱法测定六味地黄汤中梓醇含量

目的:利用高效液相色谱法分离、测定六味地黄汤中梓醇,完善质量控制方法。方法:用krom asil ODS-1柱(200 mm×4.6 mm)色谱柱,0.5%的乙腈溶液为流动相,检测波长为210 nm,柱温25℃。结果:梓醇在0.0673~1.346μg范围内呈良好的线性关系,平均回收率为97.95%,RSD=0.96%(n=5)。结论:该法方便简便、快速,准确可靠。     

增液汤不同配伍对梓醇含量的影响

目的比较增液汤的几种配伍中梓醇含量的差异。方法方法以反向高效液相色谱法(RP-HPLC)测定增液汤不同配伍情况下的各处方中梓醇的含量。结果地黄单味药梓醇煎出量为1.13%,其他配伍煎出量为1.21%~1.51%。结论地黄与其他药味合煎可促进梓醇的煎出,其中与麦冬合煎梓醇的煎出量最高。     

HPLC同时测定益精丸中梓醇和阿魏酸的含量

目的建立测定益精丸中梓醇和阿魏酸含量的HPLC方法。方法色谱条件:色谱柱:Atlantis T3 C18色谱柱(4.6 mm×250 mm,5μm);混合流动相:乙腈(A)-0.1%磷酸(B)梯度洗脱;流速:0.8 ml/min;检测波长:210 nm;柱温:32℃。结果梓醇和阿魏酸分别在6.24~124.80μg/ml(r=0.9997,n=6),4.96~99.20μg/ml(r=1.0000,n=6)范围内线性关系良好,平均回收率分别为95.2%、95.3%,RSD分别为1.3%和1.8%。结论该方法简单、快速、准确可靠,可用于测定益精丸中梓醇和阿魏酸含量。     

Catalpol prevents the loss of CA1 hippocampal neurons and reduces working errors in gerbils after ischemia-reperfusion injury

Catalpol, an iridoid glycoside, contained richly in the roots of Rehmannia glutinosa, was found for the first time to be of neuroprotection in gerbils subjected to transient global cerebral ischemia. Catalpol (1 mg/kg ip) used immediately after reperfusion and repeatedly at 12, 24, 48 and 72 h significantly rescued neurons in hippocampal CA1 subfield and reduced working errors during behavioral testing. The neuroprotective efficacy of catalpol became more evident when the doses of catalpol were increased to 5 and 10 mg/kg. In addition, it was exciting that the significant neuroprotection by catalpol was also evident when catalpol was applied up to 3 h after ischemia. But the neuroprotective efficacy of catalpol became weak when catalpol was given at 6 h after ischemia. Of great encouragement was the finding that the neuroprotection of catalpol could be seen not only in a short post-ischemic period (12 days) but also in a long period (35 days). All these indicated that catalpol was truly neuroprotective rather than simply delayed the onset of neuronal damage and might be of therapeutic value for the treatment of global cerebral ischemia.     

地黄的化学成分研究

目的：研究生地黄的化学成分。方法通过柱层析、薄层制备等手段分离得到7个化合物并用多种波谱方法鉴定了结构。结果7个化合物分别是梓醇（Ⅰ）、甘露醇（Ⅱ）、黑曲霉糖（Ⅲ）、蔗糖（Ⅳ）、glutinosalactone A（Ⅴ）、齐墩果酸（Ⅵ）、香草酸（Ⅶ）。结论化合物Ⅵ为首次在地黄块根部位分离得到,化合物Ⅶ为首次在地黄中分离得到,化合物Ⅲ为首次在地黄属植物中分离得到。     

HPLC同时测定左归丸中的5种成分

目的 采用HPLC法同时测定左归丸中尿囊素、薯蓣皂苷元、梓醇、麦角甾苷和吉奥诺苷B1的含量.方法 采用依利特C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.2％磷酸溶液,梯度洗脱,流速1.3 mL· min-1,柱温为30℃,检测波长为224(尿囊素)、210(薯蓣皂苷元和梓醇)和330(麦角甾苷和吉奥诺苷B1)nm.结果 尿囊素、薯蓣皂苷元、梓醇、麦角甾苷和吉奥诺苷B1的线性范围分别为7.270～145.4μg·mL-1(r=0.9999)、5.150～103.0 μg·mL-1(r=0.9992)、5.730～114.6 μg·mL-1(r =0.9998)、6.740 ～134.8 μg·mL-1(r =0.9995)和5.100～102.0 μg·mL-1(r =0.9997);其平均加样回收率分别为98.37％、96.92％、99.11％、96.65％、98.31％,RSD分别为0.89％、1.28％、1.10％、0.57％、1.34％(n=6).结论 所用方法简便快捷、回收率好、重复性好,可用于左归丸中尿囊素、薯蓣皂苷元、梓醇、麦角甾苷和吉奥诺苷B1的含量测定.     

HPLC法测定百合固金丸中梓醇的含量

目的:建立百合固金丸中梓醇的含量测定方法.方法:色谱柱:kromasil C18柱(4.6 mm×250 mm,5μm),流动相:水-乙腈(99.5:0.5),检测波长:210 nm,流速:1.0 mL·min-1.结果:梓醇线性范围为3.14-31.4μg·mL-1,r=0.9996,回收率为98,68%,RSD%为1.27%.结论:本方法简便、可靠、重现性较好,能起到控制百合固金丸中梓醇含量的作用.