江西省表观健康猪群携带猪链球菌状况分析

目的：了解江西省表观健康猪群携带猪链球菌的情况,为有效防控猪链球菌病提供科学依据。方法选取景德镇市、崇仁县为采样点,采集表观健康猪群鼻咽拭子和扁桃体进行猪链球菌病原分离鉴定。结果共采集表观健康猪群鼻咽拭子和扁桃体样本301份,检出2株猪链球菌2型,其毒力基因mrp、sly、ef均为阴性。结论本次调查中江西省表观健康猪携带猪链球菌的带菌率为0.66％。     

The Morphology and Electrophysiology of the Cochlea of the Miniature Pig

To report the cochlear morphology and electrophysiology of Chinese experimental miniature pigs. Twenty Chinese experimental miniature pigs were used in this study. Auditory brainstem responses (ABR), cochlear endolymphatic potentials (EP), and the potassium concentrations of cochlear endolymph were recorded. Hair cell morphology was examined using electron microscopy. The capsule of cochlea of the miniature pig has three and one‐half turns which contains a 39‐mm long membranous labyrinth. The organ of Corti in the labyrinth encompasses three rows of outer hair cells and one row of inner hair cells. The stereocilia of the hair cells in the apical turn of the cochlea were significantly longer than those in the basal turn. The vestibular apparatus consists of three semicircular canals and the otolith organs. The average threshold of the ABR was 35–45 dB SPL (n = 20) from 4 to 32 kHz. There was no significant difference in the threshold or latency of the ABR between 1‐day‐old and 30‐day‐old miniature pigs. The average EP value was 77.3 ± 14 mV (n = 9) and the average potassium concentration was 147.1 ± 13 mM (n = 5) recorded from the second turn of the cochlea. These studies on the cochlear morphology and electrophysiology of the miniature pigs help to establish the Chinese experimental miniature pig as an animal model for future studies in otology and audiology. Anat Rec, 298:494–500, 2015. © 2014 Wiley Periodicals, Inc.     

经球囊导管肝段热凝固CT与病理对照研究

目的观察经球囊导管阻断肝动脉高热灌注生理盐水行肝段热凝固CT表现。方法实验猪6头,选用球囊导管阻断肝段动脉血流进行热灌注,导管流出温度设置50~60℃之间。热灌注结束后即刻、14天,行肝动脉DSA检查和肝脏螺旋CT扫描,14天将实验猪全部处死,取出肝脏进行病理检查。结果热灌注后14天,6头实验猪均存活。DSA检查见靶段动脉闭塞,肝组织热灌注区显示充盈缺损。肝脏热灌注区CT影像学表现:热灌注结束后即刻,热灌注区显示大片状低密度改变,无明显强化,边缘显示模糊;热灌注后14天,CT平扫示肝脏热灌注区呈典型楔形低密度改变;CT增强示热灌注区显示"三环征",由内向外分为低密度无增强区、边缘强化带和移行区透明带。病理上取得6个热凝固灶,CT上低密度无增强区、边缘强化带和移行区透明带分别代表病理的凝固性坏死区、炎性充血带和纤维化带。结论增强CT能准确反映经动脉导管肝段介入性热毁损的病理改变,DSA价值有限。     

小型猪Barx1基因的克隆及其在牙胚中的定量表达

目的 观察小型猪牙齿发育相关基因Barx1片段在胚胎发育40 d(E40)不同牙胚中的定量表达,探讨Barx1基因空间表达量与牙齿形态的关系.方法 应用基因克隆技术克隆小型猪Barx1基因片段;应用激光捕获显微切割技术精确分离胚胎40 d小型猪第一乳切牙、乳尖牙、第三乳前磨牙、乳磨牙牙胚;提取微量RNA,以甘油醛-3-磷酸脱氢酶为对照,通过实时定量聚合酶链反应检测Barx1的相对表达量.结果 得到小型猪Barx1基因698个碱基的片段,Barx1在第一乳切牙、乳尖牙、第三乳前磨牙、乳磨牙中的相对表达量为0.000 249、0.000 715、0.026 096、0.1 12 656,在牙弓中由前向后呈递增趋势.结论 Barx1基因表达量与牙齿形态发育存在一定关系,推测小型猪Barx1基因可能与牙尖的分化或牙尖数目相关.     

Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs

The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium alginate microspheres (100-300 μm), a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the right hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models.     

长寿地区成年女性骨密度测量结果评估

目的 检测广西巴马地区20岁～60岁成年女性的骨密度,探讨其骨密度变化的规律.方法 随机选取当地268位20岁～60岁成年女性,按不同年龄分组,每组5岁,共8组.应用韩国生产跟骨超声骨密度测定仪,测定跟骨骨密度.采用SPSS16.0对数据进行分析.结果 268例全部进入分析.(1)女性的峰值骨密度处于36岁～40岁年龄组,峰值组的骨密度高于绝经后组(P＜0.05),且随年龄的增加呈现先升高后下降的趋势.(2)女性的骨密度随年龄的增加而减少,导致骨质疏松症的发病率升高.(3)Pearson相关分析显示,女性的SI和年龄呈现出负相关(r=-0.193,P＜0.01),和身高有显著关系(r=0.157,P＜0.05),并未发现与体重和BMI有关系.结论 广西巴马地区20岁～60岁女性SI值与年龄和身高均有显著相关性,本研究获得的SI值为进一步探讨长寿地区成年女性的骨密度状况提供参考依据.     

四个品种小型猪血液学指标测定及比较

目的 分析比较四个品种小型猪的血液学生物学特性.方法 分别选取上海地区饲养的巴马小型猪、贵州小型猪和版纳小型猪及陆川小型猪,每个品种雌雄各20头,3～6月龄,测量其血液生理、血清生化与血凝指标,然后进行单因素方差分析,并进行数据比较.结果 四个品种的小型猪血液生理14个指标差异有统计学意义(P＜0.05),血清生化12个指标组间差异有统计学意义(P＜0.05),血凝4个指标差异都有统计学意义(P＜0.05).分别对各品种群体数据进行比较分析,巴马小型猪群体雌雄间血液常规、血清生化和血凝指标差异显著(P＜0.05)的指标分别有6项、4项和3项,而贵州小型猪、版纳小型猪和陆川小型猪雌雄间差异指标较少或差异不显著.四品种小型猪相同性别间比较,也有较多指标差异显著.结论 测定和比较的巴马小型猪、贵州小型猪、版纳小型猪和陆川小型猪在血液学、血清生化和血凝等方面数据,为应用和选择小型猪开展相关生物医学和药物研究提供了基础数据.     

Detection of type 2 herpes simplex virus in cells of spermatogenic epithelium in infected testes of guinea pigs

We developed a model of herpetic orchitis in guinea pigs. Intratesticular inoculation of type 2 herpes simplex virus suspension results in infection of the testicular spermatocytes and spermatides. The possibility of viral infection dissemination from infected into intact testis is proven. __________ Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 144, No. 7, pp. 79–83, July, 2007     

注射用糜蛋白酶中组胺类物质检查方法学研究

【摘要】目的：建立注射用糜蛋白酶中组胺类物质检查方法。 方法：采用组胺检查法对注射用糜蛋白酶中组胺类物质进行限度检查方法学研究,并与现行猫法进行比较研究。结果：加标回收率86%～117%;豚鼠法方法特异性(真阴性率)100%,灵敏度(真阳性率)70%,猫法方法特异性100%,灵敏度70%;四个实验室室内重复性分别为82%、75%、83%、81%;实验室间重复性为92.9%～96.4%.结论：该方法准确与猫法相当、重复性好,可作为降压物质检查法的替代方法。     

注射用益气复脉(冻干)安全性评价

目的 通过豚鼠离体回肠模型及小鼠类致敏实验评价注射用益气复脉（冻干,YQFM）的安全性。方法 建立豚鼠离体回肠模型,依次考察0.2、0.4、0.6、0.8、1.2、1.4、1.6 mg/mL质量浓度的YQFM对静息离体肠、乙酰胆碱（Ach）及组胺（His）诱导的离体肠收缩的抑制率;雄性ICR小鼠随机分为阴性对照组,阳性对照组（C48/80,25 mg/kg）,YQFM低、高剂量（780、3 135 mg/kg,分别为临床等效剂量的0.85和3.43倍）组,分为尾iv含0.4%伊文思蓝（EB）的药液及不含0.4% EB的药液两部分进行体内类致敏反应实验,依次比较各组小鼠耳廓蓝染情况;ELISA法测定血清中His和5-羟色胺（5-HT）的含量;并对耳、肺组织进行HE染色及病理检查。结果 体外实验显示,YQFM既能够抑制离体肠的自主收缩,又能抑制Ach和His导致的离体肠痉挛;体内实验表明,YQFM低剂量组耳廓蓝染率,His、5-HT含量均在正常范围,组织病理检查未见明显异常;3.43倍临床等效剂量时耳廓蓝染率,His、5-HT含量略高,病理结果见轻微炎症反应。结论 YQFM的安全性基本良好,临床使用时需对使用剂量稍加注意,以防轻微不良反应发生。