B-type natriuretic peptide (
BNP) combats cardiac stress by reducing blood pressure and ventricular fibrosis. Human
BNP is inactivated by unknown cell surface proteases. N-terminal cleavage of mouse
BNP by the renal protease meprin A was reported to increase inactivating degradation by a second protease named neprilysin. Since the sequence surrounding the meprin A cleavage site in
BNP differs between species, we tested whether meprin A degrades human
BNP. Using a recently developed proteolytic bioassay, the ability of various protease inhibitors to block the inactivation of
BNP was measured. In rat kidney membranes, inhibitors of meprin A or neprilysin partially or completely blocked inactivation of rat
BNP1-32 when added individually or in combination, respectively. In contrast, neither inhibitor alone or in combination prevented the inactivation of human
BNP1-32 by human kidney membranes. Leupeptin, a serine protease inhibitor, totally blocked inactivation of human
BNP by human membranes, substantially blocked the inactivation of rat
BNP1-32 by human membranes, but had no effect on the inactivation of rat
BNP1-32 by rat kidney membranes. Purified neprilysin reduced the bioactivity of rat
BNP1-32 and human
BNP. Digestion with both meprin and neprilysis caused the greatest reduction in rat
BNP1-32 but had no effect on the bioactivity of human
BNP1-32. We conclude that meprin A does not degrade
BNP in humans and should not be considered a pharmacologic target of the natriuretic peptide system.
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