推按运经仪为主治疗结石300例

采用推按运经仪加中药、耳穴贴压等综合治疗结石３００例，６０例肝胆结石中，治愈２８例，显效２０例，无效１２例，有效率８０％；２４０例泌尿系结石中，治愈１３６例，显效８８例，无效１６例，有效率９３．３％。     

Smith-Fineman-Myers综合征的候选基因GPC4分析

目的:探讨GPC4基因与中国山东Smith-Fineman-Myers综合征(SFMS)的关系,并分析SFMS患者GPC4基因突变。方法:利用primer3设计扩增GPC4全部编码序列及内含子和外显子接头序列的引物,采用PCR扩增结合PCR产物直接测序方法检测GPC4基因开放性阅读框架区域基因突变。结果:在GPC4基因开放性阅读框架区域内并未检测到导致疾病的基因突变。结论:山东SFMS家系患者不是由于GPC4基因编码区域基因突变所致。     

THE INFLUENCE OF MULTIPLE FACTORS ON GALL BLADDER PRESSURE AFTER AURICULAR POINT STIMULATION

The study has explored 11 correlative variables which might affect the gallblad-der pressure GBP of patients with gall stone after auricular electrical stimulation by means of multiplestepwise regression. It was found that the size of gallbladder stone (X9), cholecystolithiasis (X4),sex (X1), and the baseline of GBP (X10) could affect the GBP change range (Y1) depressingly orreinforcingly. Also the baseline of GBP (Y1) could be affected by the variables X1 (sex), X2 (age),and X6 (long diameter of the gallbladder, LDGB), etc.. The result shows that the change in GBPafter auricular electrical stimulation is comprehensively influenced by multiple factors. And it providessome useful information for predicting the curative effect of auricular therapy on gallbladder stone inclinic.     

人胎盘组织液抗炎药效学研究

目的研究人胎盘组织液的主要药效作用。方法建立二甲苯所致小鼠耳肿胀及大鼠棉球肉芽肿的药理模型，观察人胎盘组织液对炎症模型动物的抗炎作用。结果与模型对照组相比，连续7d肌肉注射0．1mL/kg剂量的人胎盘组织液对二甲苯所致小鼠耳肿胀起到明显抑制作用，且差异显著（P〈0．01）；连续6d肌肉注射0．1mL／kg剂量的人胎盘组织液对大鼠棉球肉芽组织增生起到明显抑制作用，且差异显著（P〈0．01）。结论人胎盘组织液具有明显的抗炎作用。     

对耳轮过度前突畸形的整复

对耳轮过度前突为对耳轮的角度过锐，致使耳轮的位置相应后移，表现为与招风耳完全相反的畸形。畸形虽不十分明显，但影响美观。自１９９２年４月开始应用患侧耳廓软骨和耳后皮瓣对５例患者８只外耳进行治疗得到满意效果。认为文中所述方法是矫正对耳轮过度前突畸形的良好方法。     

毛细管电泳技术快速检测p53基因点突变

目的：探讨利用毛细管电泳技术快速检测p53基因点突变的临床应用价值。方法：采用毛细管电泳作SSCP分析，检测20例结肠癌肿瘤标本p53基因第7外显子PCR扩增产物，并与传统的聚丙烯酰胺凝胶电泳结果进行比较，最后用DNA序列测定判断其准确性。结果：20例结肠癌标本中，毛细管电泳技术检测出5例标本(Ca4，Ca6，Ca7，Ca8，Ca14)有突变，而凝胶电泳结合银染技术仅检出4例标本(Ca4，Ca6，Ca7，Cal4)有突变，测序证实经毛细管电泳所检出的5例标本均存在点突变。结论：对于PCR产物的单链构象多态性分析，毛细管电泳技术是一种更加快速、简便、敏感的方法，可应用于临床筛查基因点突变。     

Principles for reconstruction of traumatic ear defects

Summary Traumatic defects of the auricle are quite common. The eventual success of the repair is considerably influenced by the initial treatment. The different parts of the auricle require different approaches. This paper presents our methods for repairing damage to the cartilaginous frame using autologous ear cartilage grafts, and local flaps from the mastoid region to cover soft tissue defects.     

A modified approach to identification of the sickle cell anemia mutation by means of allele-specific polymerase chain reaction.

The allele-specific PCR approach has been modified by introducing a second mismatch at the 3'-penultimate link of the primer and used to identify the sickle cell anemia mutation (A-->T transversion in the sixth codon of the human beta-globin gene causing Glu-->Val substitution in the protein), thus obviating the problem of an interpretationally ambiguous 3'-terminal mismatch including T residue.     

Haplotype analysis in gelsoiin-related amyloidosis reveals independent origin of identical mutation (G654A) of gelsolin in Finland and Japan

Familial amyloidosis, Finnish type (FAF) (gelsolin-related amyloidosis) is an autosomal dominant form of systemic amyloidosis characterized by corneal lattice dystrophy and peripheral polyneuropathy. The accumulating protein in FAF consists of fragments of gelsolin, an actin-modulating protein. The gelsolin mutation G654A has been found in both Finnish and Japanese patients. To study the origin of the gelsolin mutation in these patients we performed haplotype analysis in 10 Finnish and 2 Japanese FAF families. Poymorphic DNA markers GSN, D9S103, AFMa061xd9, and AFMa139xb9 revealed a uniform disease haplotype in all the disease-associated chromosomes of the Finnish FAF families, which was different from the one observed in the Japanese families. The present results and the previously detected gelsolin mutation G654T in Czech and Danish FAF patients suggest that nucle otide 654 may represent a mutation hot spot in the gelsolin gene. The DNA markers studied here will be useful in future genealogical analyses of FAF. © 1995 Wiley-Liss, Inc.     

Description of Heart-Rate Variability Data in Accordance With a Physiological Model for the Genesis of Heartbeats

A survey is presented of techniques which transform heart-rate variability data into a signal that is both visually informative and accessible for analysis. The Instantaneous Heart-Rate (IHR) signal is introduced, i.e. the signal having the value of the heart rate (inverse interbeat interval) during the interval concerned. The IHR signal differs from the standard Delayed Heart-Rate (DHR) signal, which is always one beat late. The relationship is discussed between the different representation methods and the Integral Pulse Frequency Modulation (IPFM) model, the latter being a physiologically plausible model for the transformation of a continuous input signal (e.g., nervous influence on the cardiac pacemaker) into a series of events (heartbeats). It is shown that when the IHR signal is used as input of the IPFM model, the event series from which the signal was derived appears at the output. Hence, if the IPFM model is accepted as a model of the pacemaker, the IHR signal may be considered as an approximation of the neural (sympathetic and parasympathetic) influence on the pacemaker. In addition we show that the appearance of the IHR signal is less affected by trigger errors or extrasystoles than the standard DHR signal. It is concluded that the most attractive time-domain representation of physiological event series consists of the IHR signal, because this signal, being conceptually and computationally simple, is consistent with the IPFM model.